Single-cell analyses of intestinal epithelium reveal the dysregulation of gut immune microenvironment in systemic lupus erythematosus by Qiaolin Wang, Yutong Wu, Lianlian Ouyang, Xiaoli Min, Meiling Zheng, Lingyu Gao, Xiaoyun Chen, Zhi Hu, Shuang Yang, Wenjuan Jiang, Sujie Jia, Qianjin Lu, Ming Zhao

“…The primary findings from scRNA-seq were further validated by quantitative polymerase chain reaction (qPCR), flow cytometry, and in vivo experiments including selenium supplementation. …”
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Abundant geographical divergence of Clostridioides difficile infection in China: a prospective multicenter cross-sectional study by Lulu Bai, Telong Xu, Wenzhu Zhang, Yajun Jiang, Wenpeng Gu, Wei Zhao, Yang Luan, Yanfeng Xiong, Nianli Zou, Yalin Zhang, Ming Luo, Jinxing Lu, Bike Zhang, Yuan Wu

“…The positivity rate of toxigenic C. difficile using real-time fluorescence quantitative PCR test of feces was 10.2% (156/1528), and 125 (8.2%, 125/1528) strains were successfully isolated. …”
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Patient-centered outcomes for gastrointestinal cancer care: a scoping review protocol by Natalie G Coburn, Frances C Wright, Alyson L Mahar, Lesley Gotlib Conn, Julie Hallet, Amy T Hsu, Paul D James, Joanna Yang, Tori Barabash, Luckshi Rajendran, Claire Ludwig, Ekaterina Kosyachkova, Julie Deleemans

“…The data from full texts will be extracted, charted and summarised both quantitatively and qualitatively.Ethics and dissemination No ethics approval is required for this scoping review. …”
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Maternal Care Behavior and Care for Children Under Two during the First 1000 Days of Life in Relation to Stunting Incidence in Simoro Village, Sigi Regency by Nur Rosmala, Jusman Rau Muh, Dwi Larasati Rahma, Bengan Laba Stefiani, H Muh Rusydi, Rahmawati, Jamaluddin, Thursina R Erina

“…This study aims to investigate the maternal care behaviors of pregnant women and mothers of children under two years old during the first 1000 days of life in relation to the incidence of stunting in Simoro Village. This quantitative research uses a cross-sectional approach. …”
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Butyric Acid Modulates Gut Microbiota to Alleviate Inflammation and Secondary Bone Loss in Ankylosing Spondylitis by Ke You, Lianjun Yang, Zhihai Su, Jun Shen, Xinyang Fan, Yuanqing Guo, Zhen Yuan, Hai Lu

“…Various techniques, including an enzyme-linked immunosorbent assay (ELISA), magnetic resonance imaging (MRI), micro-CT, histology, quantitative PCR (qPCR) for intestinal tight junction protein expression, and 16S rDNA sequencing to analyze gut microbiota abundance, were employed to assess the inflammation and bone health in the target tissues. …”
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Study on the driving factors of watershed runoff change in Zuli River by Budyko hypothesis and soil and water assessment tool model by Yun Zhao, Rui Zhang, Heping Shu, Yaxian Li, Zhi Xu, Qiang Wang

“…The Mann-Kendall and Pettitt test methods were applied to analyze the mutation annual change and evolution of runoff from 1957 to 2022, the elasticity theory was applied to conduct sensitivity analysis on land use types, and the soil and water assessment tool (SWAT) model and Budyko hypothetical theory were utilized to quantitatively assess the driving factors behind runoff change. …”
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Consequences of changing regional integration on environmental development, agricultural markets, and food security by L.A. Omarbakiyev, S.M. Kantarbayeva, A.K. Nizamdinova, S.T. Zhumasheva, G.Zh. Seitkhamzina, A. Saulembekova

“…Therefore, this article aimed to examine the sustainable agriculture practices implemented in Kazakhstan’s agro-industrial complex to improve food security and reduce the environmental impact of agriculture.METHODS: To collect information, a mixed research strategy combining qualitative and quantitative methods was used. The collection of information was carried out in the summer of 2022 and consisted of three stages. …”
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The Effect of Motivation and Compensation as Well as Leadership Style on the Performance of Employees of the Population and Civil Registration Office of Samarinda City with Job Sa... by Otoh Sanusi, Eddy Soeginto K, Eka Yudhyani

“…Purpose: The Effect Of Motivation And Compensation As Well As Leadership Style On The Performance Of Employees Of The Population And Civil Registration Office Of Samarinda City With Job Satisfaction As An Intervening Variable Design/methodology/approach: using a quantitative approach. In this study, the data used are primary data and secondary data. …”
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Automatic detection of floating instream large wood in videos using deep learning by J. Aarnink, J. Aarnink, T. Beucler, T. Beucler, M. Vuaridel, V. Ruiz-Villanueva, V. Ruiz-Villanueva

“…With the growing availability of flood-related videos featuring wood uploaded to the internet, this methodology facilitates the quantification of wood transport across a wide variety of data sources.…”
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DES modified silica gel as dispersing material for miniaturized matrix solid phase dispersion applied to triazoles determination by Susanna Della Posta, Valeria Gallo, Emanuele Limiti, Marcella Trombetta, Monica Gherardi, Alessandra Gentili, Laura De Gara, Chiara Fanali

“…For each analyte the calculated limit of quantification was similar or lower than their Maximum Residue Limit. …”
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Evaluating the Accessibility of Urban Public Open Spaces Based on an Improved 2SFCA Model: A Case Study Within Chengdu’s Second Ring Road by Ling Jian, Xiaojiang Xia, Yinbing Zhao, Yang Zhang, Yuanqiao Wang, Yi Tang, Jie Chang, Changliu Wang

“…Traditional Two-Step Floating Catchment Area (2SFCA) models often lack sufficient quantitative analysis regarding the supply of urban public service facilities and population demand. …”
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Current Updates on Molecular Diagnostic Assays Used for Detection of <i>Candida auris</i>: A Systematic Review by River Chun-Wai Wong, Alfred Lok-Hang Lee, Ingrid Yu-Ying Cheung, Viola Chi-Ying Chow, Margaret Ip, Christopher Koon-Chi Lai

“…The findings were produced through narrative synthesis, with quantitative analysis conducted where applicable. <b>Results</b>: Starting from 1115 records, 28 studies that met the inclusion criteria were included in the analysis. …”
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Metallothionein 1B attenuates inflammation and hepatic steatosis in MASH by inhibiting the AKT/PI3K pathway by Canghai Guan, Xinlei Zou, Wujiang Shi, Jianjun Gao, Chengru Yang, Yifei Ge, Zhaoqiang Xu, Shaowu Bi, Xiangyu Zhong

“…This study investigates the role and potential mechanisms of metallothionein 1B (MT1B) in MASH through bioinformatics analysis and experimental validation. quantitative reverse transcription PCR and Western blot analyses confirm that MT1B expression is significantly downregulated in liver tissues of MASH patients, in high-fat diet–induced mouse models, and in hepatocytes induced by FFAs. …”
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Role of Gender in Shaping Consumer Responses to Doctor Influencer: Insights into Brand Perceptions and Repurchase Intentions in the Indonesian Mineral Water Industry by Endang Dwi Amperawati, Rahmawati Rahmawati, Endang Hariningsih, Wing Wahyu Winarno, Diana Airawaty, Agus Dwianto

“…Reisa's endorsement, the survey collects quantitative data to analyze how these attributes, along with gender as a moderating variable, influence brand perception in the digital marketing context. …”
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Serotonin-Exacerbated DSS-Induced Colitis Is Associated with Increase in MMP-3 and MMP-9 Expression in the Mouse Colon by Menglu Chen, Lei Gao, Pan Chen, Dandan Feng, Yalin Jiang, Yongchao Chang, Jianjun Jin, Fong-Fong Chu, Qiang Gao

“…MMPs and tissue inhibitors of MMPs (TIMPs) mRNA were measured by real-time quantitative RT-PCR in mouse colon and in human Caco-2 cells and neutrophils. …”
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Preservation of cfRNA in cytological supernatants for cfDNA & cfRNA double detection in non‐small cell lung cancer patients by Yidan Ma, Yifei Wang, Lei He, Jun Du, Lin Li, Zhixin Bie, Yuanming Li, Xiaomao Xu, Wei Zhou, Xiaonan Wu, Li Yang, Jing Di, Chenyang Li, Xiaoguang Li, Dongge Liu, Zheng Wang

“…Methods A protective solution (PS) was applied for preserving cfRNA in cytological supernatant (CS), and the quality of protected cfRNA was assessed by cycle threshold (CT) values from reverse transcription quantitative polymerase chain reaction (RT‐qPCR). Furthermore, we collected an additional set of malignant cytological and matched tumor samples from 84 NSCLC patients, cfDNA & cfRNA extraction and double detection for driver gene mutations was validated using the multi‐gene mutations detection by RT‐qPCR. …”
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A CRISPR/Cas12a electrochemical biosensing to detect pig mtDNA D-loop for ensuring food authenticity by Muhammad Ihda H.L. Zein, Clianta Yudin Kharismasari, Ari Hardianto, Salma Nur Zakiyyah, Riezki Amalia, Mehmet Ozsoz, Mara Mirasoli, Irkham, Yeni Wahyuni Hartati

“…These parameters enabled the development of an ultrasensitive biosensor with detection and quantification limits of 4 fM and 71 fM, respectively. …”
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Aberrant Expression of MicroRNA-15a and MicroRNA-16 Synergistically Associates with Tumor Progression and Prognosis in Patients with Colorectal Cancer by Guangfa Xiao, Huihuan Tang, Wei Wei, Jian Li, Liandong Ji, Jie Ge

“…As a result, we found that miR-15a and miR-16 expression, detected by quantitative real time-PCR, were both significantly downregulated in colorectal cancer tissues compared with adjacent colorectal mucosa (both P<0.001). …”
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Assessment of the Utility of Selected Inflammatory Markers in Correlation with Magnetic Resonance Enterography (MRE) Findings in the Diagnosis of Crohn’s Disease by Justyna Lorenc-Góra, Dariusz Waniczek, Zenon P. Czuba, Mariusz Kryj, Zbigniew Lorenc, Małgorzata Muc-Wierzgoń

“…We performed the analysis of cytokine concentrations in blood serum using the Bio-Plex Multiplex system (Bio-Rad), and their correlations with radiological parameters were assessed by magnetic resonance enterography (MRE), and fecal calprotectin levels were measured quantitatively by ELISA and clinical evaluation according to the Crohn’s Disease Activity Index (CDAI). …”
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The Influence of Gastric Microbiota and Probiotics in <i>Helicobacter pylori</i> Infection and Associated Diseases by Jagriti Verma, Md Tanveer Anwar, Bodo Linz, Steffen Backert, Suneesh Kumar Pachathundikandi

“…Several studies have documented qualitative and quantitative changes in the microbiota’s composition in the presence or absence of this pathogen. …”
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