Iguratimod Inhibits the Aggressiveness of Rheumatoid Fibroblast-Like Synoviocytes by Jin Lin, Ye Yu, Xuanwei Wang, Yini Ke, Chuanyin Sun, Lihuan Yue, Guanhua Xu, Bei Xu, Liqin Xu, Heng Cao, Danyi Xu, Nancy Olsen, Weiqian Chen

“…The mRNA expression of matrix metalloproteinases (MMPs) and proinflammatory cytokines in RA-FLSs were measured by quantitative PCR and ELISA. To gain insight into the molecular signaling mechanisms, we determined the effect of iguratimod on the activation of mitogen-activated protein kinases (MAPK) signaling pathways by the cellular thermal shift assay (CETSA) and western blot. …”
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The responsiveness of surgical research to Māori in Aotearoa, New Zealand—a scoping reviewResearch in context by Jamie-Lee Rahiri, Noah Appleby, Makayla Kahi, Annaliese Wheeler, Jason Tuhoe, Shanthi Ameratunga, Rachelle Love, Wiremu MacFater, Matire Harwood

“…Findings: A total of 254 studies were included, most being quantitative (N = 230, 91%) and most categorised under General Surgery (N = 139, 55%). …”
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Notch-Hes1 Signaling Regulates IL-17A+γδ+T Cell Expression and IL-17A Secretion of Mouse Psoriasis-Like Skin Inflammation by Yanqin Wang, Xinxin Li, Xiaoyun Xing, Haibo Xue, Ruiqun Qi, Hong Ji, Lei Ma

“…Flow cytometry detected IL-17A+γδ+T cell percentage. Quantitative real-time RT-PCR detected Hes1 mRNA expression. …”
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Validation of Immersion Precooling and Drip Sanitation Standard Operating Procedures on Weight, Temperature, Microbiological Quality, and Safety of Chicken Carcasses by Angela B.P.S. Fernandes, Clair F.S. Pereira, Hiago M. Pereira, Marco A.C. Batista, Luiz F.S. Dionisio, Amauri A. Alfieri, Bruna Alexandrino, José C. Ribeiro-Júnior

“…Despite the notable SSOP effect on the quantification of microbiological indicators during immersion cooling, the high occurrence of different pathogens underlies the need to review procedures and techniques not only in slaughterhouses but also throughout the production chain, acting in an integrated manner to provide biosecurity and reduce risks to the consumption of chicken meat.…”
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Diversity of frontier processes in Amazonian subnational jurisdictions: Frontier metrics reveal major patterns of human–nature interactions by Guido Briceño, Julie Betbeder, Agnès Bégué, Guillaume Cornu, David Katz-Asprilla, Marie-Gabrielle Piketty, Solen Le Clech, Vinicius Silgueiro, Hélène Dessard, Lilian Blanc

“…Identifying the differences and similarities between units and associated changes in forest cover due to deforestation, degradation and regrowth is crucial for context-specific management and planning. Methods for quantitatively characterizing this complexity across large agricultural frontiers are still underdeveloped. …”
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Zero-Shot Remote Sensing Scene Classification Based on Automatic Knowledge Graph and Dual-Branch Semantic Correlation Supervision by Chao Wang, Jiajun Yang, Tanvir Ahmed, Yang Zhao, Tong Zhang, Bing Sun, Tao Xie, Jie Wang, Tianyu Chen

“…However, current graphs rely heavily on expert manual interpretation, making them susceptible to human biases and difficult to expand. They also lack quantitative description of the degree of spatial relationship associations and fail to adequately represent semantic information. …”
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Therapeutic Effects of GDF6-Overexpressing Mesenchymal Stem Cells through Upregulation of the GDF15/SIRT1 Axis in Age-Related Hearing Loss by Jiali Liu, Haisen Peng, Yuehui Liu, Chunhua Li, Wen Xie

“…Methods: Reverse transcription-quantitative PCR and western blotting were used to evaluate gene expression. …”
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Basic research for ultrasound-guided injection into skeletal muscle lesions in an experimental animal model by KiyomitsuFujimoto, TakashiKanamoto, ShunyaOtani, RyoMiyazaki, KosukeEbina, KenNakata

“…Conclusion: This experimental animal study demonstrated the potential of ultrasound to quantitatively visualize abnormalities of skeletal muscle. …”
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Transient response and analytical solution of burst phenomenon in long distance water pipeline by Zhi-Guo Tao, Ling Zhou, Yun-Jie Li, Yuan Huang, Yan-Qing Lu, Yin-Ying Hu, Rui-Ling Feng

“…The research findings can provide theoretical and practical guidance for the rapid diagnosis and quantitative analysis of burst pipes.…”
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Research trends and prospects in global riverine carbon fluxes: A bibliometric analysis by Wei Fang, Mohd Remy Rozainy Mohd Arif Zainol, Xianbao Zha, Pingping Luo

“…Future research should further investigate the index quantification, scaling algorithms, and modeling framework of RCFs. …”
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Characterizing wind, wave, and Stokes drift interactions in the upper ocean during Typhoon Doksuri using the COAWST model by Yaoyao Han, Changsheng Zuo, Zhizu Wang, Yucui Wang, Chengchen Tao, Xu Zhang, Juncheng Zuo

“…The COAWST model output, combined with empirical orthogonal function (EOF) analysis and the Ekman-Stokes number, is used to quantitatively evaluate the contributions of wind and wave effects to ocean surface flow. …”
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Spatiotemporal heterogeneity of ecosystem service interactions and their determining thresholds across distinct climate zones in the temperate desert steppe of northern China by Xin Li, Xiangdong Li, Xiaoqian Li, Zhi Dong, Xinchuang Chen, Jianying Guo, Hongxiao Liu

“…Then the food and pasture supply (FP), carbon sequestration, biodiversity maintenance, soil retention, wind and sand stabilization, water conservation, water yield, air purification, and entertainment and leisure (EL) were quantitatively assessed. The results indicated that ESs’ distribution in the desert steppe exhibited significant climate differences, with the total ESs in the semi-arid zone superior to those in the arid zone. …”
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QTL mapping and candidate gene analysis of element accumulation in rice grains via genome-wide association study and population genetic analysis by Min Li, Mengxue Gao, Jinmei Huang, Shaojuan Jin, Yamei Lv, Lin Wang, Tao Huang, Fengcai Wu, Zhijian Xu, Yinghua Pan, Zhixia Liu, Chaopu Zhang, Yuntao Liang, Lijun Meng, Jianlong Xu

“…Results We conducted genetic dissection of four toxic heavy metal elements (lead, cadmium, mercury, and chromium), one quasi metallic element (arsenic), and one essential element (selenium) in grains of 290 Xian and 308 Geng rice accessions through a genome-wide association study (GWAS) based on three statistical models and assays of element concentrations from three environments. A total of 99 quantitative trait loci (QTLs) were identified. Among these QTLs, 18.2% overlapped between/among two or more elements, indicating that some QTLs related to the accumulation of certain elements may depend on other heavy metal elements or be involved in the collaborative transport of other elements. …”
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Aerobiology matters: Why people in the community access pollen information and how they use it by Danielle E. Medek, Constance H. Katelaris, Andelija Milic, Paul J. Beggs, Edwin R. Lampugnani, Don Vicendese, Bircan Erbas, Janet M. Davies

“…Theme frequency and quantitative data were compared across pollen seasons, within and after the season, and between respondents with and without access to AusPollen information. …”
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GsMTx-4 venom toxin antagonizes biophysical modulation of metastatic traits in human osteosarcoma cells by Arianna Buglione, Giulia Alloisio, Chiara Ciaccio, David Becerril Rodriguez, Simone Dogali, Marco Luce, Stefano Marini, Antonio Cricenti, Magda Gioia

“…To elucidate the molecular mechanisms that translate biomechanical alterations into biochemical responses, we employed Western blotting, real-time quantitative RT-PCR, reactive oxygen species ROS assay, and the mechanosensitive channel blocker Grammostola MechanoToxin4 (GsMTx-4). …”
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The Protective Effects of Maresin 1 in the OVA-Induced Asthma Mouse Model by Guochun Ou, Qin Liu, Chengxiu Yu, Xiaoju Chen, Wenbo Zhang, Yong Chen, Tao Wang, Yongqiang Luo, Guolu Jiang, Mingmei Zhu, Hongmei Li, Mei Zeng

“…Western blotting was used to measure the protein level of IκBα, the activation of the NF-κB signaling pathway, and the expression of NF-κB downstream inflammatory cytokines. Quantitative real-time polymerase chain reactions (qRT-PCRs) were used to evaluate the expression levels of COX-2 and ICAM-1 in lung tissues. …”
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Association of tumour mutation burden with prognosis and its clinical significance in stage III gastric cancer by Ya-Lin Han, Li Chen, Xu-Ning Wang, Mao-Lin Xu, Rui Qin, Fang-Ming Gong, Peng Sun, Hong-Yi Liu, Zhi-Peng Teng, Zhao-Xia Li, Guang-Hai Dai

“…In the TCGA cohort, gene set enrichment analysis was performed, and the relationship between TMB, prognosis and clinicopathologic factors was analysed. Western blot and quantitative real-time polymerase chain reaction were used to detect the expression levels of both proteins and genes. …”
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The effects of psychosocial and economic factors on the quality of life of patients with end-stage renal disease and their caregivers in Klang Valley, Malaysia: protocol for a mixe... by Norhayati Ibrahim, Sin Yee Chu, Ching Sin Siau, Noh Amit, Rozmi Ismail, Abdul Halim Abdul Gafor

“…Despite the current circumstances, there are very few studies in Malaysia that have explored the psychosocial factors, specifically on the economic impact of the management of ESRD.Methods and analysis This two-phase sequential explanatory mixed-methods design, incorporating a quantitative design (phase I) and a qualitative study (phase II), is to be conducted in 4 government hospitals and 10 other non-governmental organisations or private dialysis centres within Klang Valley, Malaysia. …”
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Machine Learning Diagnostic Model for Hepatocellular Carcinoma Based on Liquid–Liquid Phase Separation and Ferroptosis-Related Genes by Wenchao Chen, Ting Zhu, Xiaofan Pu, Linlin Zhao, Senhao Zhou, Xin Zhong, Suihan Wang, Tianyu Lin

“…Based on central hub genes, a diagnostic model was developed through LASSO regression and validated using KM survival analysis and real-time quantitative polymerase chain reaction (RT-qPCR). Then the effects of NRAS on the development of HCC and ferroptosis were also detected. …”
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The Role of Motivation to Promote Personal and Social Responsibility Through PE and Sport: A Comparative Exploratory Investigation of Prospective PE Teachers and Sports Leaders in... by Istvan Soos, Ian Whyte, Diana Szekeres, Karoly Ozsvath, Attila Szabo, Jonathan Ling, Jose Francisco Jiménez-Parra, Alfonso Valero-Valenzuela

“…Method: A cross-sectional, cross-cultural comparative quantitative study was conducted with university students (n = 368) from Spain (n = 109 males, 67%) and Hungary (n = 108 males, 53%) using a demographic questionnaire and six validated questionnaires). …”
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