Serum Cytokines Th1, Th2, and Th17 Expression Profiling in Active Lupus Nephritis-IV: From a Southern Chinese Han Population by Keshav Raj Sigdel, Lihua Duan, Yin Wang, Weiping Hu, Ning Wang, Qingyi Sun, Qingyan Liu, Xiaocong Liu, Xianghua Hou, Ao Cheng, Guixiu Shi, Yanlin Zhang

“…The data from our study suggests that the early detection and quantification of these cytokines may help in prediction of active form of LN-IV.…”
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Reduced Presence of SARS-CoV-2 microRNA-like Small RNA in the Serum of Patients with Post-Acute Sequelae SARS-CoV-2 Infection by Maria Alfreda Stincarelli, Isabella Abbate, Giulia Matusali, Michele Tanturli, Marta Camici, Rosaria Arvia, Elisabetta Lazzari, Eleonora Cimini, Alessandra Vergori, Fabrizio Maggi, Simone Giannecchini

“…This study examined the presence of SARS-CoV-2 microRNA (miRNA)-like small RNAs in extracellular fluids and their potential link to PASC by using a quantitative stem-loop RT-PCR MiRNA assay. Initially, it was demonstrated that three previously identified SARS-CoV-2 miRNA-like small RNAs, specifically svRNA 1 and 2 and miR-07a, were significantly expressed in infected cells in vitro and released into the supernatant following infection by different SARS-CoV-2 variants. …”
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Soil moisture controls over carbon sequestration and greenhouse gas emissions: a review by Yuefeng Hao, Jiafu Mao, Charles M. Bachmann, Forrest M. Hoffman, Gerbrand Koren, Haishan Chen, Hanqin Tian, Jiangong Liu, Jing Tao, Jinyun Tang, Lingcheng Li, Laibao Liu, Martha Apple, Mingjie Shi, Mingzhou Jin, Qing Zhu, Steve Kannenberg, Xiaoying Shi, Xi Zhang, Yaoping Wang, Yilin Fang, Yongjiu Dai

“…Soil moisture directly affects photosynthesis, respiration, microbial activity, and soil organic matter dynamics, with optimal levels enhancing carbon storage while extremes, such as drought and flooding, disrupt these processes. A quantitative analysis is provided on the effects of soil moisture on CS-GHG across various ecosystems and climatic conditions, highlighting a “Peak and Decline” pattern for CO₂ emissions at 40% water-filled pore space (WFPS), while CH₄ and N₂O emissions peak at higher levels (60–80% and around 80% WFPS, respectively). …”
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68Ga-Pentixafor PET/CT for the assessment of therapeutic outcomes following superselective adrenal arterial embolization in patients with primary aldosteronism by MengDan Li, Lili Guan, Lu Yang, WenBo Li, Zhu Xia, Min Mao, Hua Pang

“…For patients with aldosterone-producing adenoma, both visual and semi-quantitative analyses of PET/CT effectively assessed changes in radioactive uptake of the lesion. …”
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Effects of sub-monthly and sub-daily water level variations on water level fluctuation requirements of Phragmites australis and Phalaris arundinacea by Tingyu Zhang, Wenjuan Gao, Saibo Yuan, Xiaodie Jiang, Yongjing Zhao, Yongde Cui, Hongzhu Wang

“…These findings provide essential quantitative data to restore aquatic vegetation and carry out effective ecological water level management in large-scale riparian ecosystems.…”
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Technology to improve reliable access to oxygen in Western Uganda: study protocol for a phased implementation trial in neonatal and paediatric wards by Graham Moore, Hamish Graham, Rami Subhi, Sheillah Bagayana, Joseph Mugerwa, David Peake, Eleanor Nakintu, Daniel Murokora, Roger Rassool, Marc Sklar, Bryn Sobott

“…Useability will be quantitatively and qualitatively evaluated in terms of acceptability, feasibility and appropriateness, using standardised implementation outcome measure tools.Ethics and dissemination Ethics approval was obtained from Mbarara University of Science and Technology (MUREC 1/7) and the University of Melbourne (2021-14489-13654-2). …”
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Defining the sensitivity of cosmic ray muons to groundwater storage changes by Tramontini, Matías, Rosas-Carbajal, Marina, Zyserman, Fabio Iván,  Longuevergne, Laurent, Nussbaum, Christophe, Marteau, Jacques

“…Furthermore, the groundwater mass quantification is usually done through proxies as few methods characterize a physical property directly linked to the volumetric water content. …”
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METTL3-dependent DLG2 inhibits the malignant progression of cervical cancer by inactivating the Hippo/YAP signaling by Mei Pu, Xia Xiao, Shasha Lv, Daqing Ran, Qian Huang, Mingming Zhou, Qirong Lei, Lingshuang Kong, Qing Zhang

“…This study aims to explore the regulatory mechanism of DLG2 in cervical cancer and its clinical implications. Methods Quantitative reverse transcription polymerase chain reaction and western blotting assays were employed to detect RNA and protein expression, respectively. …”
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A Systematic Review and Meta-Analysis of Mapping Biopsy for Primary Extramammary Paget’s Disease in Reducing Recurrence Following Surgical Excision by Thirrisha Murugan, Louis Choon Kit Wong, MD, Xing-Yi Sarah Ong, MD, Sze Huey Tan, PhD, Joey Wee-Shan Tan, MSc, Ying Liu, PhD, Nicholas B. Shannon, PhD, MD, Jianbang Chiang, MBBS, MRCP, MMed, Eileen Poon, MBBS, MRCP, MMED, Jason Yongsheng Chan, MBBS, MRCP, MMed, FAMS, PhD, Valerie Shiwen Yang, MB BChir, PhD, MRCP, FAMS, Nagavalli Somasundaram, MBBS, MRCP, MMED, Mohamad Farid, MBBS, MRCP, MMed, Ru Xin Wong, MBBS, FRCR, Wen Long Nei, MBBS, FRCR, Jin Wei Kwek, MBBS, FRCR, FAMS, Choon Hua Thng, MBBS, FRCR, FAMS, Tiffany Hennedige, MBBS, FRCR, MMed, MCI, Po Yin Tang, BMEDSc, MBBS, FRCPath, FRCAPA, Sathiyamoorthy Selvarajan, MBBS, PGDCP, FRCPath, MCS, FAMS, Kae Jack Tay, MBBS, MRCS, MMed, MCI, FAMS, Mohamed Rezal Abdul, MB, BCh, BAO, LRCP&SI, MSurg, Jolene Si Min Wong, MBBS, MMed, FRCS, Chin Jin Seo, MB, BCh, BAO, MMed, FRCS, Khee Chee Soo, MBBS, MD, FRACS, FACS, Claramae Shulyn Chia, MBBS, MMed, FRCS, Chin-Ann Johnny Ong, MBBS, MMed, FRCS, PhD

“…Additional study results not included in the quantitative assessment were qualitatively assessed and reported. …”
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Genome-wide characterization of the MADS-box gene family in Paeonia ostii and expression analysis of genes related to floral organ development by Xueting Yang, Qi Guo, Shaodan Liu, Duoduo Wang, Dingding Zuo, Tongfei Niu, Dongfeng Wei, Lili Guo, Xiaogai Hou

“…Analysis of the cis-acting elements revealed that the 110 PoMADS genes contained different kinds of hormones and stress-related cis-acting elements in their promoter regions. Quantitative real-time PCR analysis was employed to validate the expression patterns of some PoMADS genes related to floral organ development. …”
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Efflux Pump Activity and Mutations Driving Multidrug Resistance in Acinetobacter baumannii at a Tertiary Hospital in Pretoria, South Africa by Noel-David Nogbou, Granny M. Nkawane, Khanyisa Ntshane, Charles K. Wairuri, Dikwata T. Phofa, Kagiso K. Mokgokong, Mbudzeni Ramashia, Maphoshane Nchabeleng, Lawrence C. Obi, Andrew M. Musyoki

“…Conventional PCR amplification of the AdeABC efflux pump (adeB, adeS, and adeR) and quinolone (parC and gyrA) resistance genes were performed, followed by quantitative real-time PCR of AdeABC efflux pump genes. …”
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The truth about co-benefits: a multidimensional feasibility assessment for thailand and beyond by Eric Zusman, Kaoru Akahoshi, Tatsuya Hanaoka, Christopher S Malley, Supat Wangwongwatana, Nutthajit Onmek, Ittipol Paw-armart, Kim Oanh Nguyen Thi, Lai Nguyen Huy, Johan C I Kuylenstierna, Tomoki Hirayama, Yurie Goto, Kawashima Kazumasa, Markus Amann, Zbigniew Klimont, Jessica Slater

“…This study extends insights from research focusing on quantitatively assessing the feasibility of a 1.5 °C future to build a multidimensional framework for measuring different barriers to achieving co-benefits. …”
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Challenges in Predicting the Filtration Performance of a Novel Sewn Mask: Scale-up from Filter Holder to Mannequin Measurements by Audrey J. Dang, Benjamin M. Kumfer, J. Tyler Bertroche, Jane Olson Glidden, Christopher R. Oxford, Udayabhanu Jammalamadaka, Mary Ruppert-Stroescu, Alexander R. Scott, Jason A. Morris, Connie Gan, Jesse Hu, Bradley King, David I. A. Dhanraj, Shruti Choudhary, Pratim Biswas, Richard L. Axelbaum, Kathleen W. Meacham, Brent J. Williams

“…Sewn from sterilization wrap, our mask (sealed to a mannequin head with silicone) filters 85 ± 1% of 136 nm particles (NaCl, 85 L min−1) and passes quantitative fit tests for 4 of 6 volunteers, representing intermediate protection between a surgical mask and N95 FFR. …”
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Gonadal aspects, testosterone and estradiol profiles of the Lebranche mullet Mugil liza subjected to different temperatures during the larviculture phase by Marco Shizuo Owatari, Vanessa Martins da Rocha, Cleize Sales da Silva, Marina Cesconeto dos Santos, Mariana Rangel Pilotto, Fabiola Santiago Pedrotti, Caio Magnotti, Maurício Laterça Martins, Vinicius Ronzani Cerqueira

“…The present study evaluated the influence of different temperatures during Mugil liza larviculture and their late effects on gonadal development and hormonal quantification after 24 months. The fertilized eggs (45 eggs L-1) were initially kept in a 60 L circular tank until hatching. …”
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Improving Media Literacy Among Higher Education Students Through Vitagenic Information by Darmonjon Maxmudova, Egambergan Khudoynazarov, Meruert Pazilova, Khairulla Alyaminov, Gulbahar Abilova, Zamira Sherimbetova, Sherzod Korabayev

“…The study employs a mixed-methods methodology, integrating both qualitative and quantitative data. Surveys were conducted with 200 university students to evaluate their existing media literacy levels and to determine how vitagenic experiences affect their critical thinking abilities. …”
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Interleukin 10 Plays an Important Role in Neonatal Rats with Hypoxic-Ischemia Associated with B-Cell Lymphoma 2 and Endoplasmic Reticulum Protein 29 by Xue Bai, Liu-Lin Xiong, Chang-Le Fang, Hao-Li Zhou, Lu-Lu Xue, Yue Hu, Qing-Jie Xia, Jia Liu, Jun-Yan Zhang, Ting-Hua Wang, Si-Jin Yang

“…The expression of IL-10 in the cortex, hippocampus, and lung tissues was measured with immunohistochemistry, real-time quantitative polymerase chain reaction (RT-qPCR), and western blot (WB). …”
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Geodetic Evidence of the Interannual Fluctuations and Long-Term Trends Over the Antarctic Ice Sheet Mass Change by Yuanjin Pan, Xiaohong Zhang, Jiashuang Jiao, Hao Ding, C. K. Shum

“…In this study, these two aspects were quantitatively analyzed through global positioning system (GPS) and gravity recovery and climate experiment/follow on (GRACE/GFO) over the past two decades. …”
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Grem1 inhibits osteogenic differentiation of MBMSCs in OVX rats through BMP/Smad1/5 signaling pathway by Shan-shan Yang, Min LinHu, Xiao-hua Hu, Si-si Jiang, Wen-yue Hu, Xiao-hong Yang

“…Techniques such as Western Blot (WB) and Real-Time Quantitative PCR (RT-qPCR) were employed to determine changes in Grem1 expression in MBMSCs of postmenopausal rats and its effect on osteogenic differentiation. …”
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Influence of age, sex, and presence of cleft lip and palate on the quantity and quality of saliva-derived DNA by Lina M. Escobar, Zita Bendahan, Deisy Abril, David Díaz-Baez, Adolfo Paternina, Farith González-Martinez, Ma Clara González-Carrera

“…This study aimed to compare saliva DNA concentrations from individuals of different ages with and without CL/P.Saliva samples were collected, DNA was extracted and the quantification was performed by spectrophotometry. Kruskal-Wallis test was used to compare frequencies between groups, and Spearman's rho for correlations.We obtained saliva DNA from 314 individuals: 107 with CL/P (mean age: 12.7 ± 6.5 years), 103 parents of children with CL/P (mean age: 41.1 ± 9.3 years), 52 individuals without CL/P under 18 years old (controls), and 52 individuals without CL/P over 18 years old (controls). …”
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Blood levels of cytokines highlight the role of inflammation in Alzheimer's disease by Lorenzo Campanelli, Pablo Galeano, Federico A. Prestia, Carolina Cuesta, Maria C. Dalmasso, María Flores-López, Cristian Gona, Nicolás Irureta, Claudia Kairiyama, Julieta Lisso, Antonio Jesús López-Gambero, Ines Mintz, Nancy Medel, Karen S. Campuzano, Carolina Muchnik, Gisela V. Novack, Natividad Olivar, Ivana Quiroga, Nerea Requena-Ocaña, Jose Antonio Reyes-Bueno, Pedro Serrano-Castro, Zulma Sevillano, Patricia Solis, Juan Suárez, Ivana Villella, Nancy Wukitsevits, Eduardo M. Castaño, Fernando Taragano, Silvia Kochen, Daniel G. Politis, Luis I. Brusco, Fernando Rodríguez de Fonseca, Laura Morelli

“…After applying strict filters for quantification limits, only 13 analytes were included in the analysis. …”
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