Removal of Matrix Interferences by Nano-MgO and Co-Adsorbents for Accurate Multi-Pesticide Residue Analysis in the Chinese Medicinal Herb, Paeoniae Radix Alba by Chunyu Wang, Xinquan Wang, Jiao Wang, Shanshan Di, Zhiwei Wang, Hao Xu, Huiyu Zhao, Changshan Zhao, Peipei Qi

“…Various PRA samples were extracted using 8 mL 0.5% acetic acid-acetonitrile solution and purified by a dispersive solid-phase extraction method with 30 mg nano-MgO, 40 mg primary secondary amine (PSA), and 40 mg octadecylsilane (C18) as the cleanup adsorbents, followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). 70.7% of pesticides showed a weak matrix effect after the purification process, indicating that this method can give the precise quantitative analysis of trace pesticides residue. The method was systematically validated under optimal conditions in five different kinds of PRA samples; good linearity was observed in the concentration range of 0.5–250 μg/L or 1–250 μg/L. …”
Get full text

Retinoic Acid Signal Negatively Regulates Osteo/Odontogenic Differentiation of Dental Pulp Stem Cells by Jiangyi Wang, Guoqing Li, Lei Hu, Fei Yan, Bin Zhao, Xiaoshan Wu, Chunmei Zhang, Jinsong Wang, Juan Du, Songlin Wang

“…Alkaline phosphatase (ALP) activity assays, alizarin red staining, quantitative calcium analysis, CCK8 assay, osteogenesis-related gene expression, and in vivo transplantation were conducted to determine the osteo/odontogenic differentiation potential and proliferation potential of DPSCs. …”
Get full text

Cloning and expression characterisation of LiDXS2 gene in Oriental Lily (Lilium 'Sorbonne') by Yu Jiaxuan, Wu Nana, Fu Ruyu, Xue Lili, Zhang Jinzhu, Dong Jie, Yang Tao, Fan Jinping

“…To reveal its regulatory effect on floral scents of lily, LiDXS2 was cloned from Oriental Lily ‘Sorbonne’; the protein location was determined using subcellular localisation; and the expression pattern in different flowering stages and tissues was probed by fluorescence quantitative PCR. The plant expression vector was constructed to transform Arabidopsis thaliana, and the transgenic plants were characterised. …”
Get full text

Serum Neuropeptide Y Levels Are Associated with TNF-α Levels and Disease Activity in Rheumatoid Arthritis by Melissa Ramirez-Villafaña, Ana M. Saldaña-Cruz, Javier A. Aceves-Aceves, Edsaul E. Perez-Guerrero, Nicté S. Fajardo-Robledo, Edy D. Rubio-Arellano, Cesar A. Nava-Valdivia, Maria O. Carrillo-Escalante, Sylvia E. Totsuka-Sutto, David Cardona-Müller, Betsabe Contreras-Haro, Mario Salazar-Paramo, Ernesto G. Cardona-Muñoz, M. Huerta, Jorge I. Gamez-Nava, Norma A. Rodriguez-Jimenez, Laura Gonzalez-Lopez

“…This study encourages the quantification of NPY levels as a surrogate marker for RA-active. …”
Get full text

Therapeutic Effects of BCG Vaccination on Type 1 Diabetes Mellitus: A Systematic Review and Meta-Analysis of Randomized Controlled Trials by Yu-Chen Chang, Chien-Ju Lin, Yu-Hsuan Hsiao, Yu-Han Chang, Shu-Jung Liu, Hsin-Yin Hsu

“…The results of HbA1c and fasting C-peptide levels were extracted for further quantitative assessment. The pooled meta-analysis demonstrated no significant difference in HbA1c levels (mean difference [MD], −0.12; 95% confidence interval [CI], −0.53 to 0.30; I2=56%) or fasting C-peptide levels (MD, −0.15; 95% CI, −0.35 to 0.06; I2=0%) in the BCG intervention group as compared with that in the placebo group. …”
Get full text

Cis‐Regulation of an m6A Eraser by an Insertion Variant Associated with Survival of Patients With Non‐Small Cell Lung Carcinoma by Lei Cheng, Qiangsheng Hu, Yanan Wang, Wei Nie, Haijiao Lu, Bo Zhang, Genming Zhao, Shiyun Ding, Feng Pan, Yinchen Shen, Runbo Zhong, Ruoxin Zhang

“…Here, 7273 expression quantitative trait loci potentially regulating 30 m6A pathway genes are identified from the GTEx database, with 69 single nucleotide polymorphisms significantly associated with survival of non‐small cell lung carcinoma (NSCLC) patients (n = 1523) from the ongoing genome‐wide association study after false positive probability tests. …”
Get full text

Knockout of <i>dhx38</i> Causes Inner Ear Developmental Defects in Zebrafish by Mengmeng Ren, Xiang Chen, Liyan Dai, Jiayi Tu, Hualei Hu, Xiaohan Sun, Jiong Luo, Pei Li, Yiyang Fu, Yuejie Zhu, Weiqiang Sun, Zhaohui Tang, Mugen Liu, Xiang Ren, Qunwei Lu

“…Immunofluorescence and semi-quantitative RT-PCR were employed to test apoptotic cells and abnormal splicing. …”
Get full text

Diabetic Distal Symmetrical Polyneuropathy: Correlation of Clinical, Laboratory, and Electrophysiologic Studies in Patients with Type 2 Diabetes Mellitus by Yi-Ching Weng, Sung-Sheng Tsai, Rong-Kuo Lyu, Chun-Che Chu, Long-Sun Ro, Ming-Feng Liao, Hong-Shiu Chang, Chiung-Mei Chen, Jawl-Shan Hwang, Hung-Chou Kuo

“…This cross-sectional study is aimed at determining the prevalence of distal symmetrical polyneuropathy (DSPN) and diabetic peripheral neuropathic pain (DPNP) in participants with type 2 diabetes mellitus (T2DM); finding the risk factors for DSPN and DPNP via biochemical tests; and correlating DSPN and DPNP with the results of electrophysiologic studies, quantitative sensory tests, and neurologic examination. …”
Get full text

Safety and performance of oropharyngeal muscle strength training in the treatment of post-stroke dysphagia during oral feeding: protocol for a systematic review and meta-analysis by Xin Wang, Ying Wang, Fenghua Zhou, Minxing Gao, Lingyuan Xu, Heying Wang, Jinan Song, Xiaoqiu Yang

“…Any disagreements during this process will be resolved by discussion or by consultation with a third author. Next, quantitative or qualitative, subgroup and sensitivity analyses of the included literature data will be performed as appropriate.Ethics and dissemination Ethical approval is not required for this systematic review as no primary data collection will be required. …”
Get full text

DeepGlioSeg: advanced glioma MRI data segmentation with integrated local-global representation architecture by Ruipeng Li, Yuehui Liao, Yueqi Huang, Xiaofei Ma, Guohua Zhao, Yanbin Wang, Chen Song

“…Test-time augmentation (TTA) and volume-constrained (VC) post-processing are subsequently applied to refine the final segmentation outputs.ResultsExtensive experiments were conducted on three publicly available glioma MRI datasets and one privately owned clinical dataset. The quantitative and qualitative findings consistently show that DeepGlioSeg achieves superior segmentation performance over other state-of-the-art methods.DiscussionBy integrating CNN- and Transformer-based features in parallel and adaptively emphasizing underrepresented tumor regions, DeepGlioSeg effectively addresses the challenges associated with glioma heterogeneity and imbalanced region distributions. …”
Get full text

In Vivo Magic Angle Magnetic Resonance Imaging for Cell Tracking in Equine Low-Field MRI by Carolin Horstmeier, Annette B. Ahrberg, Dagmar Berner, Janina Burk, Claudia Gittel, Aline Hillmann, Julia Offhaus, Walter Brehm

“…Image analysis comprised blinded evaluation and quantitative assessment of signal-to-noise ratio. The magic angle technique enhanced the tendon signal-to-noise ratio (P<0.001). …”
Get full text

Adapting the World Health Organization’s Surgical Safety Checklist to High-Income Settings: A Hybrid Effectiveness-Implementation Trial Protocol by Nathan Turley, MA, MBA, Karolina Kogut, BA, Barbara Burian, PhD, Rachel Moyal-Smith, DrPH, PA-C, James Etheridge, MD, MPH, Yves Sonnay, MScPH, William Berry, MD, MPH, Alan Merry, MD, MBChB, Alexander Hannenberg, MD, Alex B. Haynes, MD, MPH, Roger D. Dias, MD, PhD, MBA, Kathryn Hagen, MD, George Molina, MD, MPH, Lisa Spruce, DNP, RN, Carla Williams, RN, MHSM, Mary E. Brindle, MD, MPH, FRCSC

“…This mixed-methods study includes quantitative and qualitative data derived from surveys, semi-structured interviews, patient focus groups, and SSC performance observations. …”
Get full text

Peripheral Regulatory Cells Immunophenotyping in Kidney Transplant Recipients with Different Clinical Profiles: A Cross-Sectional Study by Janette Furuzawa-Carballeda, Guadalupe Lima, Perla Simancas, Dolores Ramos-Bello, Margaret Simancas, Ian C. Bostock, Mario Vilatobá, Bernardo Gabilondo, Julio Granados, Luis Morales-Buenrostro, Josefina Alberú, Luis Llorente

“…IL-10-producing B cells, CD4+/CD25hi, and CD8+/CD28− Tregs, as well as CCR6+/CD123+/IDO+ DCs, were quantitated by flow cytometry. IL-10-producing Bregs (immature/transitional, but not CD19+/CD38hi/CD24hi/CD27+B10 cells), CCR6+/CD123+/IDO+ DCs, and Tregs from ELTGF patients had similar or higher percentages versus HD (P<0.05). …”
Get full text

Development of Collagen-Based 3D Matrix for Gastrointestinal Tract-Derived Organoid Culture by Joo Hyun Jee, Dong Hyeon Lee, Jisu Ko, Soojung Hahn, Sang Yun Jeong, Han Kyung Kim, Enoch Park, Seon Young Choi, Sukin Jeong, Joong Woon Lee, Han-Jun Cho, Min-Soo Kwon, Jongman Yoo

“…Then, characteristics of mouse colon organoids were analyzed by morphology and quantitative messenger RNA (mRNA) expression, revealing that the mouse colon organoids grown in the collagen-based matrix and in Matrigel had quite similar morphology, specific markers, and proliferative rates. …”
Get full text

A pilot mixed methods randomized control trial investigating the feasibility and acceptability of a culturally tailored intervention focused on beliefs, mistrust and race-congruent... by Meng-Jung Wen, Martha Maurer, Annika L. Pickard, Makenzie Hansen, Olayinka O. Shiyanbola

“…Mixed methods integration was implemented using a side-by-side joint display to compare, synthesize and interconnect the quantitative and qualitative results across all feasibility domains.ResultsThirteen participants (93%) completed the trial, demonstrating high adherence and retention. …”
Get full text

Investigating Diagnostic Accuracy of Strip Kit (Rapid Test) for Detecting Aflatoxin M1 in Milk Based on the Concentration Obtained by ELISA and HPLC by Hanieh Amiri, Naser Morgan Azghadi, Azadeh Saki, Zeinab Rezaei, Ali Asghar Najafpoor, Mahsa Khakpour, Mansooreh Soltani

“…The limit of detection (LOD) was set at 100 ppt (ng/kg) and 50 ppt for Strip Kit and ELISA, respectively, and the limit of quantification (LOQ) was 5 ppt for confirmatory HPLC. …”
Get full text

Comparative Oil Composition Study of the Endemic Moroccan Olive (Olea europaea subsp. maroccana) and Wild Olive (var. Sylvestris) in Central West Morocco by Sara Elgadi, Ahmed Ouhammou, Hamza Zine, Nadia Maata, Rachid Ait Babahmad, Abderraouf El Antari

“…Analysis of variance and principal component analysis (PCA) of the chemical composition highlighted a significant difference between the quantitative and qualitative properties of their oil. …”
Get full text

Divergent response of grassland aboveground net primary productivity and precipitation utilization efficiency to altered precipitation patterns by process-based model by Chen Cheng, Lu Wu, Lu Wu, Hongyan Liu, Boyi Liang, Xinrong Zhu, Feiyun Yang

“…This study aims to quantitatively assess the impact of different precipitation scenarios on grassland productivity using the APSIM model. …”
Get full text

Challenges of Research Performance Evaluation in Iran: A Qualitative Study by Nadia Saniee, Shahram Sedghi, Abdolreza Noroozi chakoli, Salime Goharinezhad, Leila Nemati anaraki

“…The most critical challenges included a lack of normalization in research evaluation, lack of suitable scientometric training, lack of importance of scientometrics in research management, the necessity to change the scientometricians role, absence of scientometrician in research policy-making, the focus of universities ranking systems on research indicators, the emphasis of universities ranking systems on quantity, the over-focus on quantitative indicators, the incompleteness of scientometric indicators, the lack of proper application of scientometric indicators, the role of scientometricians in improving the research quality, and the need for balanced attention to the quantity and quality of research. …”
Get full text

Exploration of Secondary Metabolites in <i>Platostoma menthoides</i> (L.) Using Ethyl Acetate Extract and Its Antibacterial, Antioxidant, and Larvicidal Activities by Pavithra Senthilkumar, Subbu Thavamurugan, Aravinth Annamalai, Prabhu Kolandhasamy, Vasanthy Muthunarayanan, Nandhini Selvaraj, Lakshmiprabha Azhagiyamanavalan, Ramachandran Vinayagam

“…For the chemical profiling, a GC–MS analysis of the extract was conducted, and it showed the presence of various phytoconstituents, and the FT-IR spectrum revealed the functional groups associated with them. The quantitative phytochemical estimations revealed values of 34.87 ± 0.53 mg of GAE equivalents/g and 22.19 ± 1.11 mg of QUE equivalents/g, respectively, for total phenolic and flavonoid content. …”
Get full text