Role of bovine respiratory syncytial virus in etiology of respiratory diseases on milk farms by S. V. Koteneva, A. V. Nefedchenko, T. I. Glotova, A. G. Glotov

“…The virus was seldom detected in trachea and bronchial mucosa (1.1%) and in pulmonary lymph nodes (0.8%). Quantification of BRSV RNA demonstrated that maximum virus accumulation was observed in lungs and nasal charges and it confirms data on its tropism to pulmonary interstitium.…”
Get full text

Robust proteome profiling of cysteine-reactive fragments using label-free chemoproteomics by George S. Biggs, Emma E. Cawood, Aini Vuorinen, William J. McCarthy, Harry Wilders, Ioannis G. Riziotis, Antonie J. van der Zouwen, Jonathan Pettinger, Luke Nightingale, Peiling Chen, Andrew J. Powell, David House, Simon J. Boulton, J. Mark Skehel, Katrin Rittinger, Jacob T. Bush

“…We report a versatile, label-free quantification proteomics platform for competitive profiling of cysteine-reactive fragments against the native proteome. …”
Get full text

NRBF2 plays a crucial role in the acquisition process of learning and memory, independent of the Vps34 complex by Songfen Wu, Songfen Wu, Songfen Wu, Haicai Zhuang, Haicai Zhuang, Haicai Zhuang, Xidan Zhou, Kuan Li, Kuan Li, Kuan Li

“…IntroductionNRBF2, a component of autophagy-associated PIK3C3/VPS34-containing phosphatidylinositol 3-kinase complex, plays a crucial role in learning and memory processes, yet its specific impact on memory and the underlying molecular mechanisms remains unclear.MethodsHere, we utilized NRBF2 knockout mice to examine its influence on the time course of fear memory. Employing quantitative PCR, Western blot analysis, behavioral tests, and electrophysiology, we investigated the mechanisms through which NRBF2 affects memory processing.ResultsWe observed an increase in Nrbf2 mRNA levels at 6 and 12 h, and protein levels at 6 h post fear conditioning. …”
Get full text

Autophagy-related long non-coding RNA MIR210HG plays a therapeutic role in hepatocellular carcinoma by Chaoqun Zhang, Dianxing Sun, Huifang Zhou, Chao Liu, Jie Ruan, Jiwen Kang, Ying Xie

“…Kaplan–Meier and Cox regression analyses were conducted to assess survival correlations. Quantitative reverse transcription PCR was used to measure lncRNA MIR210HG expression in liver cancer cells and normal liver cells. …”
Get full text

Enhancing Thermal Comfort in Historic Buildings by Wind-Driven Ventilation Systems—A Case Study of the Praterateliers in Vienna by Aida Shayegani, Viera Joklova, Katarina Kristianova, Juraj Illes

“…The selected analysis period (May to September) captures the peak thermal stress conditions in Vienna, with wind rose diagrams and temperature characteristics providing insights into the ventilation potential during these months. The quantitative results demonstrate that cross-ventilation, combined with windcatchers and subterranean air exchange systems, improved thermal comfort metrics—such as predicted mean vote indices—by up to 30%, particularly in windward and leeward zones. …”
Get full text

Potato resistance to quarantine diseases by A. V. Khiutti, O. Yu. Antonova, N. V. Mironenko, T. A. Gavrilenko, O. S. Afanasenko

“…Mechanisms of potato quantitative resistance to S. endobioticum and PGN and adaptation processes in pathogens populations resulting in overcoming resistance of host are discussed. …”
Get full text

Human health risk assessment of aluminium in water treated with aluminium-based coagulants in a rural area by Fernando García-Ávila, Tatiana Encalada-Alvear, Luis M. Alvarez-Altamirano, Alex Avilés-Añazco, Lorgio Valdiviezo-Gonzalez, Emigdio Alfaro-Paredes

“…The risk assessment was carried out using the Hazard Index (HI), which allows the quantification of the potential risk associated with exposure to aluminium through drinking water. …”
Get full text

A Convenient and Sensitive LC-MS/MS Method for Simultaneous Determination of Carbadox- and Olaquindox-Related Residues in Swine Muscle and Liver Tissues by Heying Zhang, Wei Qu, Yanfei Tao, Dongmei Chen, Shuyu Xie, Lingli Huang, Zhenli Liu, Yuanhu Pan, Zonghui Yuan

“…The limits of detection (LODs) of the six analytes were determined to be 0.01 μg·kg−1 to 0.25 μg·kg−1, and the limits of quantification (LOQs) were 0.02 μg·kg−1 to 0.5 μg·kg−1. …”
Get full text

A Review on Deep Learning for Quality of Life Assessment Through the Use of Wearable Data by Vasileios Skaramagkas, Ioannis Kyprakis, Georgia S. Karanasiou, Dimitris I. Fotiadis, Manolis Tsiknakis

“…Traditional QoL assessment methods, often reliant on subjective reports or informal questioning, face challenges in quantification and standardization. To address these challenges, DL, a branch of machine learning inspired by the human brain, has emerged as a promising tool. …”
Get full text

Ensemble Deep Learning Object Detection Fusion for Cell Tracking, Mitosis, and Lineage by Imad Eddine Toubal, Noor Al-Shakarji, D. D. W. Cornelison, Kannappan Palaniappan

“…Cell tracking and motility analysis are essential for understanding multicellular processes, automated quantification in biomedical experiments, and medical diagnosis and treatment. …”
Get full text

Catheter-directed therapy for pulmonary embolism in pediatrics: a systematic review and meta-analysis by Basel F. Alqeeq, Dina Essam Abo-elnour, Mohamed Rifai, Luis L. Gamboa, Ibrahim Qattea, Mohammed Hamzah, Khaled M. Al-Farawi, Alaa Ahmed Elshanbary, Ibrahim Kamal, Mohammed Alsabri

“…Results Sixteen case reports/series were included in the quantitative analysis with a total population of 40 children diagnosed with PE. …”
Get full text

Comparison of HER2-Targeted Antibodies for Fluorescence-Guided Surgery in Breast Cancer by Solmaz AghaAmiri, Jo Simien, Alastair M. Thompson, Julie Voss, Sukhen C. Ghosh, Servando Hernandez Vargas, Sarah Kim, Ali Azhdarinia, Hop S. Tran Cao

“…Methods. mAbs were conjugated to the near-infrared fluorescent (NIRF) dye, IRDye800, and for quantitative in vitro assessment, to the radiometal chelator, desferrioxamine, to enable dual labeling with 89Zr. …”
Get full text

Accumulation of advanced oxidative protein products exacerbate satellite glial cells activation and neuropathic pain by Chen Tu, Shi-Cheng Wang, Meng-Xuan Dai, Si-Qi Lai, Zhi-Wei Huang, Yong-Peng Yu, Yun-Biao Chen, Ji-Huan Zeng, Liang Wang, Zhao-Ming Zhong

“…Additionally, oxidative stress levels and inflammatory cytokine production in SGCs, triggered by AOPPs exposure, were measured through the DCFH-DA probe for ROS detection and ELISA kits for cytokine quantification. Results CCI significantly elevated the AOPPs levels in the plasma and sciatic nerve and caused AOPPs accumulation in the DRG. …”
Get full text

Toll-Like Receptors 2, 4, and 7, Interferon-Gamma, Interleukin 10, and Programmed Death Ligand 1 Transcripts in Leishmanin Skin Test-Positive Reactions of Ibizan Hound Dogs by Laura Ordeix, Sara Montserrat-Sangrà, Pamela Martínez-Orellana, Laia Solano-Gallego

“…Immune gene expressions were examined by quantitative PCR (qPCR) analysis. LST-positive reactions presented significant upregulation of TLR2, TLR4, IL-10, IFN-γ, and PD-L1 and downregulation of TLR7 when compared with healthy skin of seronegative control dogs from a nonendemic area. …”
Get full text

The safety and efficacy of cannabinoids for the treatment of mental health and substance use disorders: protocol for a systematic review and meta-analysis by Jack Wilson, Andrew Langcake, Zachary Bryant, Tom P. Freeman, Janni Leung, Gary C. K. Chan, Amir Englund, Myfanwy Graham, Emily Stockings

“…Therefore, the current systematic review will (a) identify all RCTs examining the efficacy of cannabinoids in treating MH and SUD, (b) provide a quantitative or narrative synthesis of the evidence examining efficacy, and (c) synthesise adverse event data to examine evidence of harm. …”
Get full text

Implementation of the STEPPS program for the treatment of self-harm behaviors in Catalonia’s prisons: barriers and facilitators by Rosa Lorente-Català, Irene Jaén, Xavier Buscà-Huertas, Berta Framis, Azucena García-Palacios

“…Eight Focus Group Discussions based on the Consolidated Framework for Implementation Research (CFIR) were conducted with 17 professionals who implemented the STEPPS program. Additionally, quantitative data were gathered using an open-closed questionnaire based on a CFIR questionnaire. …”
Get full text

Extreme high temperatures and heatwave events across Europe in 2023 by Yuqing Sun, Gensuo Jia, Xiyan Xu

“…However, there is a lack of quantitative analysis of spatial and temporal features of such record high heatwaves. …”
Get full text

Determination of arsenic and cadmium uptake by rice from topsoil by Desheng Yang, Zhechuan Liu, Wenqi Zhao, Ting Du, Shengyang Fan, Zhuang Xiong, Shaobing Peng, Xiaoxiao Li, Fei Wang

“…In a 2‐year field experiment conducted in central China from 2016 to 2017, the significant role of topsoil in pollutant absorption, in particular the arsenic (As) and cadmium (Cd) uptake in rice was examined quantitatively. Two soil treatments, removing half of the topsoil (TR) and a control (CK), were applied alongside two rice varieties ‐ Huanghuazhan (HHZ, indica inbred) and Yangliangyou6 (YLY6, indica hybrid). …”
Get full text

Deep imaging of LepR+ stromal cells in optically cleared murine bone hemisections by Yuehan Ni, Jiamiao Wu, Fengqi Liu, Yating Yi, Xiangjiao Meng, Xiang Gao, Luyi Xiao, Weiwei Zhou, Zexi Chen, Peng Chu, Dan Xing, Ye Yuan, Donghui Ding, Ge Shen, Min Yang, Ronjie Wu, Ling Wang, Luiza Martins Nascentes Melo, Sien Lin, Xiaoguang Cheng, Gang Li, Alpaslan Tasdogan, Jessalyn M. Ubellacker, Hu Zhao, Shentong Fang, Bo Shen

“…Confocal microscopy of these transparent samples allows for qualitative and quantitative image analysis using Aivia or Bitplane Imaris software, assessing a spectrum of parameters. …”
Get full text

Development of an analytical method for the simultaneous determination of 22 Polycyclic Aromatic Hydrocarbons (PAHs) in maternal and umbilical cord blood by Swiha, Stéphanie, Pichon, Valérie, Fournier, Thierry, Gil, Sophie, Delaunay, Nathalie

“…The final sample processing procedure led to extraction yields of between 27 and 57% and between 34 and 69% for 22 PAHs in serum and umbilical cord blood, respectively, with standard deviation values of less than 11%. Limits of quantification between 0.2 and 3.1 ${\mu }$g/L were achieved for PAHs fluorescing in spiked real samples, close to the concentrations expected in biological samples.…”
Get full text