Low-Intensity Pulsed Ultrasound Modulates RhoA/ROCK Signaling of Rat Mandibular Bone Marrow Mesenchymal Stem Cells to Rescue Their Damaged Cytoskeletal Organization and Cell Biolog... by Rong Zhang, Zhaoling Wang, Guoxiong Zhu, Gaoyi Wu, Qingyuan Guo, Hongchen Liu, Lei Chen

“…In this study, the effects of LIPUS on cytoskeletal reorganization, cell viability, and osteogenic differentiation capacity of rat mandible-derived bone marrow mesenchymal stem cells (M-BMMSCs) induced by radiation were determined by immunofluorescence staining, CCK-8 cell proliferation assay, quantification of alkaline phosphatase (ALP) activity, alizarin red staining, and real-time RT-PCR, respectively. …”
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Challenges of healthcare financing in the world’s largest refugee camp: a mixed-method study among healthcare stakeholders for Rohingya refugees in Bangladesh by Isabel Smith, Imdadul Haque Talukdar, Sayeeda Tarannum, M A Rifat, Priyanka Boga, Jubayer Mumin, Pablo André Veronés, Ziba Mahdi, Syeda Saima Alam, Arif Faysal Khan

“…Financial documents (available online) and reports from respective coordinating agencies were also reviewed.Setting Online key informant interviews (KIIs) were conducted from Stockholm with participants residing in Bangladesh between June 2022 and September 2022.Participants Eight KIIs were conducted with professional health programme managers, executives and personnel involved in policy coordination and implementation in refugee camps in Cox’s Bazar district of Bangladesh.Results We identified four themes and three subthemes outlining key challenges in healthcare funding, including decreasing funds (supported by a quantitative assessment) due to macroglobal issues, conflicting short-term and long-term priorities between implementing partners, insufficient efficacy due to challenges with collaborative priority-setting and implementing common processes, and a lack of consensus regarding equity between host and refugee communities.Conclusions The study identified unique challenges beyond the commonly discussed health financing issues in a resource-deficient setting: stakeholders’ conflicting priorities regarding funding and undecided equity issues among the host and refugee communities are worth scholarly and policy focus. …”
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The Molecular Diagnosis Protocols of New Coronavirus (COVID-19); Specificity and Sensitivity an Overview by Abdullah Ahmed Hama, Othman Abdulrahman Mohammed, Fatima Mahmud Ali, Osama Hamid Shareef, Sardar Muhammad Wli, Sabiha Sharif, Syamand Ahmed Qadir

“…The new outbreak of COVID-19 can be detected by different molecular protocols. Quantitative polymerase chain reaction (qPCR) is the recommended technique used with varied sensitivity due to primers variation and specimen type. …”
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Expression Profiling Using a cDNA Array and Immunohistochemistry for the Extracellular Matrix Genes FN-1, ITGA-3, ITGB-5, MMP-2, and MMP-9 in Colorectal Carcinoma Progression and D... by Suzana Angelica Silva Lustosa, Luciano de Souza Viana, Renato José Affonso, Sandra Regina Morini Silva, Marcos Vinicius Araujo Denadai, Silvia Regina Caminada de Toledo, Indhira Dias Oliveira, Delcio Matos

“…The expression associations between ECM molecules and selected epithelial markers EGFR, VEGF, Bcl2, P53, and KI-67 have also been examined in 114 patients with colorectal cancer who underwent primary tumor resection. Quantitative real-time PCR and immunohistochemistry tissue microarray methods were performed in samples from the primary tumors. …”
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The complex social, cultural and psychological drivers of the ‘chemsex’ experiences of men who have sex with men: a systematic review and conceptual thematic synthesis of qualitati... by Edward Mundy, Alexander Carter, Tom Nadarzynski, Christopher Whiteley, Richard O. de Visser, Carrie D. Llewellyn

“…Past systematic reviews have primarily focussed on quantitative research exploring the key characteristics and health consequences of chemsex. …”
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Molecular Characterization of Ferulate 5-Hydroxylase Gene from Kenaf (Hibiscus cannabinus L.) by Jonggeun Kim, Bosung Choi, Young-Hwan Park, Byoung-Kwan Cho, Hyoun-Sub Lim, Savithiry Natarajan, Sang-Un Park, Hanhong Bae

“…Transcriptional analysis of F5H ortholog was conducted using quantitative real-time PCR during the developmental stages of various tissues and in response to various abiotic stresses. …”
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Feasibility and potential effect of a pilot blended digital behavior change intervention promoting sustainable diets over a year by Ujué Fresán, Miguel López-Moreno, Sergi Fàbregues, Paquito Bernard, Anna Boronat, Vera Araújo-Soares, Laura M. König, Joren Buekers, Guillaume Chevance

“…The intervention included text messages, and individualized online feedback sessions. Quantitative data on diet composition was collected daily for 15 weeks distributed over the year. …”
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The Role of Nature Reserves in Ecosystem Services and Urban Ecological Sustainable Development by Haoyu Wu, Fei Song, Haifu Li, Junbo Bai, Lijuan Cui, Fangli Su, Zahra Kalantari, Carla Sofia Ferreira

“…With the acceleration of global urbanization, the ecosystem services (ES) and ecological balance of nature reserves have been significantly impacted. However, quantitative assessments of the multiple contributions of nature reserves to urban ecological sustainability are still lacking. …”
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The histone demethylase KDM5C enhances the sensitivity of acute myeloid leukemia cells to lenalidomide by stabilizing cereblon by Lu Zou, Dan Cao, Qing Sun, Wenjun Yu, Bingzong Li, Guoqiang Xu, Liang Zhou

“…In this study, we explored the interactome for CRBN using proximity labeling technique TurboID and quantitative proteomics, and then investigated the antileukemia effect of Len. …”
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Determination of Mining-Induced Changes in Hydrogeological Parameters of Overburden Aquifer in a Coalfield, Northwest China: Approaches Using the Water Level Response to Earth Tide... by Qingyu Xu, Guangcai Wang, Xiangyang Liang, Shen Qu, Zheming Shi, Xianbin Wang

“…This study revealed the changes in permeability and specific storage associated with the mining disturbance which could have great significance for quantitative assessment of the impact of mining on overburden aquifer.…”
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Efficacy of Rapid Salivary C-Reactive Protein Test to Assess Early Changes in Malignancy in the Oral Cavity and Its Utility in Screening for Oral Cancer by Vathsala Patil, Ravindranath Vineetha, Komal Smriti, Kalyana Chakravarthy Pentapati, Srikanth Gadicherla, Carnelio Sunitha

“…<b>Methods:</b> In this study, unstimulated saliva samples of cases (OPMDs and oral cancer) and controls (systemically healthy subjects) were used to detect CRP levels using a novel colorimetric, quantitative rapid assay kit. Kruskal–Wallis ANOVA with a post hoc Dunn’s test were applied to determine the difference in the mean and SD values between the case and control groups. …”
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Psychological, psychiatric, and behavioral sciences measurement scales: best practice guidelines for their development and validation by Alberto Stefana, Stefano Damiani, Umberto Granziol, Umberto Provenzani, Marco Solmi, Marco Solmi, Marco Solmi, Marco Solmi, Marco Solmi, Eric A. Youngstrom, Eric A. Youngstrom, Eric A. Youngstrom, Paolo Fusar-Poli, Paolo Fusar-Poli, Paolo Fusar-Poli

“…Psychiatric, psychological, and behavioral sciences scales provide quantitative representations of phenomena such as emotions, beliefs, functioning, and social role perceptions. …”
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Detection of Gastroesophageal Reflux Esophagitis Using 2-fluoro-2-deoxy-d-glucose Positron Emission Tomography by Min-Kuei Tsai, Hueisch-Jy Ding, Hsueh-Chou Lai, Kuo-Yang Yen, Chia-Ing Li, Yu-Yi Lin, Kai-Yuan Cheng, Keh-Bin Wang, Chia-Hung Kao

“…This is a retrospective study reviewing 408 healthy check-up subjects (169 females and 239 men), who underwent both FDG-PET and upper gastrointestinal endoscopy during September 2008 to December 2009. Quantitative analysis of FDG uptake in the distal part of the esophagus was performed by calculating the maximum standard uptake value (SUVmax). …”
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Probiotics supplementation for management of type II diabetes risk factors in adults with polycystic ovarian syndrome: a meta-analysis of randomized clinical trial by Chengcheng Zhang, Yingyue Sheng, Jinchi Jiang, Yuzheng Xue, Leilei Yu, Fengwei Tian, Jianxin Zhao, Hao Zhang, Jian Jin, Qixiao Zhai

“…An analysis of pooled extracted data revealed that supplementation with probiotics significantly decreased fasting blood glucose (FBG, n = 7; standardized mean difference (SMD) = −0.40; 95 % confidence interval (CI): −2.02, −0.02; P = 0.04) and insulin levels (n = 6; SMD = −0.57; 95 % CI: −0.89, −0.25; P = 0.0004) and the homeostatic model assessment of insulin resistance (n = 7; SMD = −0.64; 95 % CI: −0.96, −0.31; P = 0.0001) while increasing the quantitative insulin sensitivity check index (QUICKI, n = 5; SMD = 0.58; 95 % CI: 0.08, 1.09; P = 0.02) in patients with PCOS. …”
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A New Method for Designing Dense Skeleton Asphalt Mixture Based on Meso Parameter by Liwan Shi, Duanyi Wang, Junfeng Wang, Zhonghai Jiang, Hehao Liang, Xiao Qin

“…VCAmix(IMAGE) ≤ VCADRC and n¯c>1.6 while C < 20% can be used as qualitative and quantitative evaluation criterion for forming better main skeleton structure of coarse aggregate. …”
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Plastic harmful ingredients reduce the level of neurofilament light chain protein by Long Li, Xiaoxiu Ma, Junwei Liu, Yongli Wu

“…Additionally, Weighted Quantile Sum model allowed us to identify the mixed effects of all 24 plastic hazardous ingredients, with Mono(2-Ethyl-5-Oxohexyl) Phthalate, Mono-isobutyl phthalate, Mon butyl Phthalate, Propyl paraben and Triclosan occupying prominent positions. …”
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Induced Pluripotent Stem Cell Derivation and Ex Vivo Gene Correction Using a Mucopolysaccharidosis Type 1 Disease Mouse Model by Toshio Miki, Ludivina Vazquez, Lisa Yanuaria, Omar Lopez, Irving M. Garcia, Kazuo Ohashi, Natalie S. Rodriguez

“…Double guide RNAs were used to remove the Neor sequence, and various lengths of donor templates were used to reconstruct the exon VI sequence. A quantitative PCR-based screening method was used to identify Neor removal. …”
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Expression of Sclerostin in Osteoporotic Fracture Patients Is Associated with DNA Methylation in the CpG Island of the SOST Gene by Yanming Cao, Bin Wang, Ding Wang, Dongxiang Zhan, Caiyuan Mai, Peng Wang, Qiushi Wei, Yamei Liu, Haibin Wang, Wei He, Liangliang Xu

“…After extracting total RNA and protein, we checked the relative expression levels of SOST by quantitative real-time PCR and western blot. Also, immunohistochemical staining was performed to observe the expression of SOST protein in the bone samples. …”
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Dysregulation of c-Jun (JUN) and FBJ murine osteosarcoma viral oncogene homolog B (FOSB) in obese people and their predictive values for metabolic syndrome by Chenxi Yang, Yi Chen, Guangfeng Tang, Tongtong Shen, Li Li

“…Herein, the expression profiles of c-Jun (JUN) and FBJ murine osteosarcoma viral oncogene homolog B (FOSB) in individuals with obesity and patients with MetS from the Gene Expression Omnibus database. Quantitative reverse transcription polymerase chain reaction (RT-qPCR) was used to evaluate the messenger RNA levels of JUN and FOSB in the peripheral blood of healthy volunteers (lean and obese) and patients with MetS (lean and obese), along with that in the adipose tissue and peripheral blood of obese mouse model. …”
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Impact of Physical and Psychological Strain on Work-Related Musculoskeletal Disorders: A Cross-Sectional Study in the Construction Industry by Abdul Kadir MSc, Riza Yosia Sunindijo PhD, Baiduri Widanarko PhD, Dadan Erwandi PhD, Sjahrul M. Nasri PhD, Bangga Agung Satrya MKM, Stevan Deby Anbiya Muhamad Sunarno PhD, Hardy Atmajaya MT, Popy Yuniar PhD, Tubagus Dwika Yuantoko MKKK, Lailatul Qomariyah PhD, Cynthia Febrina Maharani PhD

“…This cross-sectional study used a purposive sampling technique to gather quantitative data from 409 respondents working in four construction companies through structured questionnaires. …”
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