Room temperature CRISPR diagnostics for low-resource settings by Eric A. Nalefski, Selma Sinan, Jason L. Cantera, Anastasia G. Kim, Remy M. Kooistra, Rafael E. Rivera, Jordyn P. Janshen, Sanchita Bhadra, Joshua D. Bishop, Andrew D. Ellington, Ilya J. Finklestein, Damian Madan

“…To mitigate these limitations, we identify the ortholog TsCas12a as a highly active nuclease at 25 °C and develop a single-protocol RPA-Cas12a detection reaction with this enzyme. A quantitative kinetic analysis reveals that fast nuclease activation is more critical than higher steady-state trans-cleavage activity for room temperature diagnostic applications. …”
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Determination of Levetiracetam in Human Plasma by Dispersive Liquid-Liquid Microextraction Followed by Gas Chromatography-Mass Spectrometry by Greyce Kelly Steinhorst Alcantara, Leandro Augusto Calixto, Luiz Alberto Beraldo de Moraes, Regina Helena Costa Queiroz, Anderson Rodrigo Moraes de Oliveira, Cristiane Masetto de Gaitani

“…So this work presents a dispersive liquid-liquid microextraction method followed by gas chromatography-mass spectrometry (DLLME-GC-MS) for LEV quantification in human plasma. However, due to the matrix complexity a previous purification step is required. …”
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The Chloroplastic Small Heat Shock Protein Gene KvHSP26 Is Induced by Various Abiotic Stresses in Kosteletzkya virginica by Xiaohua Liu, Lizi Zhao, Jianzhao Li, Lijun Duan, Kai Zhang, Xuqiang Qiao, Weihuan Li, Chengchao Zheng, Xiaoli Tang, Hongxia Zhang

“…Amino acid sequence alignment and phylogenetic analysis demonstrated that KvHSP26 shared 30%-77% homology with other sHSPs from Arabidopsis, cotton, durian, salvia, and soybean. Quantitative real-time PCR (qPCR) assays exhibited that KvHSP26 was constitutively expressed in different tissues such as leaves, stems, and roots, with a relatively higher expression in leaves. …”
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Generation of Femtosecond Laser-Cut Decellularized Corneal Lenticule Using Hypotonic Trypsin-EDTA Solution for Corneal Tissue Engineering by Man-Il Huh, Kyoung-Pil Lee, Jeongho Kim, Soojin Yi, Byeong-Ung Park, Hong Kyun Kim

“…Of all protocols, the decellularization methods, which efficiently removed nuclear materials examined as detected by immunofluorescent staining, were quantitatively tested for sample DNA and glycosaminoglycan (GAG) contents, recellularization efficacy, and biocompatibilities. …”
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A Robust Metatranscriptomic Technology for Population-Scale Studies of Diet, Gut Microbiome, and Human Health by Andrew Hatch, James Horne, Ryan Toma, Brittany L. Twibell, Kalie M. Somerville, Benjamin Pelle, Kinga P. Canfield, Matvey Genkin, Guruduth Banavar, Ally Perlina, Helen Messier, Niels Klitgord, Momchilo Vuyisich

“…Viomega includes sample collection, ambient temperature sample preservation, total RNA extraction, physical removal of ribosomal RNAs (rRNAs), preparation of directional Illumina libraries, Illumina sequencing, taxonomic classification based on a database of >110,000 microbial genomes, and quantitative microbial gene expression analysis using a database of ~100 million microbial genes. …”
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The Secretome of Bone Marrow and Wharton Jelly Derived Mesenchymal Stem Cells Induces Differentiation and Neurite Outgrowth in SH-SY5Y Cells by Ana O. Pires, Andreia Neves-Carvalho, Nuno Sousa, António J. Salgado

“…Finally, neurite lengths assessment and quantitative real-time reverse-transcription polymerase chain reaction (RT-PCR) analysis demonstrated that CM from BMSCs and HUCPVCs differently induced neurite outgrowth and mRNA levels of neuronal markers exhibited by SH-SY5Y cells. …”
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High-Dose Neural Stem/Progenitor Cell Transplantation Increases Engraftment and Neuronal Distribution and Promotes Functional Recovery in Rats after Acutely Severe Spinal Cord Inju... by Taoyang Yuan, Qian Liu, Jie Kang, Hua Gao, Songbai Gui

“…However, the dose-related effects of the neural stem/precursor cell (NSPC) transplantation on the injury microenvironment, NSPC survival, axonal growth, neuronal distribution, the composition of neurons, oligodendrocytes, and astrocytes in the lesion area and functional recovery have not yet been quantitatively evaluated in the context of severe SCI. …”
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Effect of composting on the microbiological and parasitic load in animal production wastes in Brazil by Paula Fernanda Alves Ferreira, Júlia Ferreira Xavier, Danielli Monsores Bertholoto, Dayanne Araújo de Melo, Thaís Ribeiro Correia, Shana de Mattos de Oliveira Coelho, Miliane Moreira Soares de Souza, Marco Antônio de Almeida Leal, Ednaldo da Silva Araújo, Irene da Silva Coelho

“…Method The presence of Salmonella sp. and/or helminth eggs and the quantification of thermotolerant coliforms were determined in horse bedding and organic and conventional poultry litter during the process of composting.  …”
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Correlation of Visuospatial Ability and EEG Slowing in Patients with Parkinson’s Disease by Dominique Eichelberger, Pasquale Calabrese, Antonia Meyer, Menorca Chaturvedi, Florian Hatz, Peter Fuhr, Ute Gschwandtner

“…Furthermore, cognitive status in PD-patients correlates with quantitative EEG. This cross-sectional study aimed to investigate the correlation between EEG slowing and visuospatial ability in nondemented PD-patients. …”
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Analysis of MLKL, RIP1 and RIP3 Immunostaining Markers in Human Liver Tissue from Fatal Yellow Fever Cases: Insights into Necroptosis by Vanessa do Socorro Cabral Miranda, Luiz Fabio Magno Falcão, Hellen Thais Fuzii, Marcos Luiz Gaia Carvalho, Jeferson da Costa Lopes, Arnaldo Jorge Martins Filho, Ana Cecilia Ribeiro Cruz, Raimunda do Socorro da Silva Azevedo, Jorge Rodrigues de Sousa, Mayumi Duarte Wakimoto, Pedro Fernando da Costa Vasconcelos, Juarez Antônio Simões Quaresma

“…Using the in situ method, we evaluated the centrilobular zone (Z3), midzonal zone (Z2), periportal zone and portal tract (PT) of human liver parenchyma with markers for necroptosis, RIPK1, RIPK3 and MLKL. A quantitative analysis revealed a significantly higher expression of MLKL, RIP1 and RIP3 in the liver parenchyma of YF cases compared to controls in different zones (Z3, Z2, Z1) and portal tracts (PTs) of the liver, especially in zone 2. …”
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Potential roles for vitamin D in preventing and treating impulse control disorders, behavioral addictions, and substance use disorders: A scoping review by Laya Jalilian-Khave, Razi Kitaneh, Binah Baht Ysrayl, Anna Borelli, Melissa C. Funaro, Marc N. Potenza, Gustavo A. Angarita

“…We extracted and summarized quantitative and qualitative data through a narrative synthesis and assessed the quality of studies using the Joanna Briggs Institute (JBI) and SYRCLE (Systematic Review Centre for Laboratory Animal Experimentation) criteria. …”
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Development of Hydrophilic Interaction Liquid Chromatography Method for the Analysis of Moxonidine and Its Impurities by Slavica Filipic, Milica Elek, Marija Popović, Katarina Nikolic, Danica Agbaba

“…The obtained values of the limit of detection and quantification revealed that the method can be used for determination of impurities levels below 0.1%. …”
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Fine-Grained Lung Cancer Classification from PET and CT Images Based on Multidimensional Attention Mechanism by RuoXi Qin, Zhenzhen Wang, LingYun Jiang, Kai Qiao, Jinjin Hai, Jian Chen, Junling Xu, Dapeng Shi, Bin Yan

“…We conduct a comparative analysis of the two aspects of feature fusion and attention mechanism through quantitative evaluation metrics and the visualization of deep learning process. …”
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Developing and evaluating a translanguaging-multiliteracies learning design (LDTMP) for student agency in a college English course in China by Jie Zhou, Jie Zhou, Sze Seau Lee, Sze Seau Lee, Si Na Kew

“…In the second phase, the prototype of LDTMP was implemented in a college English course and evaluated quantitatively and qualitatively through a pilot study. …”
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Association of Interleukin-18 Gene Polymorphism with Susceptibility to Visceral Leishmaniasis in Endemic Area of Bihar, an Indian Population by Dinesh Kumar, Puja Tiwary, Jaya Chakravarty, Shyam Sundar

“…IL-18 expression in peripheral blood mononuclear cells (PBMC) collected from VL patients and controls was measured by quantitative real-time RT-PCR. Distribution of G allele at position −656 (P<0.0001) and double haplotypes GGC/GGA (P=0.05) were found to be significantly associated with controls while genotypes TT (P<0.0001) and single haplotypes TGA (P=0.0002), with cases. …”
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Cortical arealization of interneurons defines shared and distinct molecular programs in developing human and macaque brains by Xiangling Feng, Yingjie Gao, Fan Chu, Yuwen Shan, Meicheng Liu, Yaoyi Wang, Ying Zhu, Qing Lu, Mingfeng Li

“…., the employment of CRH in primate interneurons during late-fetal stage distinguishes from their postnatal emergence in mice, and our entropy quantifications manifest the interneuron diversities gradually increase along the developmental ages in human and macaque cerebral cortices. …”
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Diverse single-stranded nucleic acid binding proteins enable both stable protection and rapid exchange required for biological function by Michael Morse, Ben A. Cashen, Ioulia Rouzina, Mark C. Williams

“…The effective length of an ssNA substrate held at fixed tension is altered upon protein binding, enabling quantification of both the structure and kinetics of protein–NA interactions. …”
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Mapping QTL for Root and Shoot Morphological Traits in a Durum Wheat × T. dicoccum Segregating Population at Seedling Stage by Anna Iannucci, Daniela Marone, Maria Anna Russo, Pasquale De Vita, Vito Miullo, Pina Ferragonio, Antonio Blanco, Agata Gadaleta, Anna Maria Mastrangelo

“…“Simeto” and the T. dicoccum accession “Molise Colli” was grown in soil and evaluated for a number of shoot and root morphological traits. A total of 17 quantitative trait loci (QTL) were identified for shoot dry weight, number of culms, and plant height and for root dry weight, volume, length, surface area, and number of forks and tips, on chromosomes 1B, 2A, 3A, 4B, 5B, 6A, 6B, and 7B. …”
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Prior Knowledge of Target Direction and Intended Movement Selection Improves Indirect Reaching Movement Decoding by Hongbao Li, Yaoyao Hao, Shaomin Zhang, Yiwen Wang, Weidong Chen, Xiaoxiang Zheng

“…It was further confirmed by quantitative analysis. Combined with prior knowledge, the trajectory decoder achieved the best performance among three decoders. …”
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Experimental Study on Comprehensive Real-Time Methods to Determine Geological Condition of Rock Mass along the Boreholes while Drilling in Underground Coal Mines by Ganggang Niu, Kai Zhang, Bosong Yu, Yanlong Chen, Yu Wu, Jiangfeng Liu

“…The results indicate that the rock mass condition can be quantitatively evaluated using the mean square value of the signal and qualitatively estimated using the energy of the spectrum. …”
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