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    Open-source implementation of polarisation-resolved single-shot differential phase contrast microscopy (pDPC) on a modular openFrame-based microscope by Huihui Liu, Sunil Kumar, Edwin Garcia, William Flanagan, Jonathan Lightley, Christopher Dunsby, Paul M.W. French

    Published 2025-03-01
    “…We recently demonstrated polarisation differential phase contrast microscopy (pDPC) as a robust, low-cost single-shot implementation of (semi)quantitative phase imaging based on differential phase microscopy. pDPC utilises a polarisation-sensitive camera to simultaneously acquire four obliquely transilluminated images from which phase images mapping spatial variation of optical path difference can be calculated. pDPC microscopy can be implemented on existing or bespoke microscopes and can utilise radiation at a wide range of visible to near infrared wavelengths and so is straightforward to integrate with fluorescence microscopy. …”
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