Natural Killer Cells from Malignant Pleural Effusion Are Endowed with a Decidual-Like Proangiogenic Polarization by Annalisa Bosi, Silvia Zanellato, Barbara Bassani, Adriana Albini, Alessandra Musco, Maria Cattoni, Matteo Desio, Elisa Nardecchia, Davide Giuseppe D’Urso, Andrea Imperatori, Lorenzo Dominioni, Douglas M. Noonan, Lorenzo Mortara, Antonino Bruno

“…Natural killer (NK) cells are crucial in tumor recognition and eradication, but their activity is impaired in cancer patients, becoming poorly cytotoxic. …”
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A New Antibody–Cytokine Construct Targeting Natural Killer Cells: An Immunotherapeutic Approach to Chronic Lymphocytic Leukemia by Michela Flego, Mauro Andreotti, Francesca Romana Mauro, Maria Beatrice Arasi, Silvia Zamboni, Zuleika Michelini, Sara Pepe, Clementina Maria Galluzzo, Roberta Amici, Diego Moricoli, Chiara Mazzei, Alessandro Ascione, Alessandra Mallano

“…We designed and developed a cis-acting immunocytokine composed of an anti-CD56 single-chain Fragment variable (scFv) and IL15, labeled scFvB1IL15. scFvB1IL15 was tested in vitro on peripheral blood mononuclear cells (PBMCs) obtained from both different healthy donors (HDs) and CLL patients in order to evaluate its ability to target NK cells and enhance their activation and NK-mediated directed cytotoxicity. scFvB1IL15 specifically induced strong degranulation and cytokine and chemokine production in NK cells in both HD- and CLL patient-derived PBMC samples. …”
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Whole-cell vaccine of Streptococcus agalactiae in Oreochromis sp. with immersion method by , Sukenda, Trian Rizky Febriansyah, Sri Nuryati

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LYMPHOCYTES IN PATIENTS WITH CHRONIC ACTIVE EPSTEIN-BARR VIRUS INFECTION EXHIBITED ELEVATED PD-1/PD-L1 EXPRESSION AND A PREVAILING TH2 IMMUNE RESPONSE. by kang sun

“…Methods: Consequently, we examined the levels of programmed cell death 1 (PD-1)/programmed death ligand 1 (PD-L1) expression, the status of EBV infection, and the frequencies of peripheral lymphocyte subpopulations in 24 patients with CAEBV and 15 EBV-seronegative donors using flow cytometry. Results: Patients with CAEBV showed a notable rise in the expression levels of PD-1 and PD-L1 in peripheral T and NK cells compared to healthy donors (P < 0.05). …”
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Autologous Enhancement by Interferon of Natural Killer Activity of Human Peripheral Blood Lymphocytes by S. B. Cheknev, Y. G. Ashmanova, A. D. Pritsker, O. L. Latysheva, F. I. Yershov, A. J. Kulberg

“…Autologous IFN-α and IFN-γ did not augment NK activity of PBL from healthy donors in vitro, whereas in samples from MS patients the IFNs strongly stimulated NK cell cytotoxic function. …”
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Effect of HLA-G5 Immune Checkpoint Molecule on the Expression of ILT-2, CD27, and CD38 in Splenic B cells by Hana Rohn, Cordula Lang, Sabine Schramm, Falko M. Heinemann, Mirko Trilling, Anja Gäckler, Oliver Witzke, Peter A. Horn, Vera Rebmann

“…B cells are of particular interest in the context of the HLA-G-ILT-2 interaction because the ILT-2 receptor is constitutively expressed on most B cells, whereas it is only present on some subsets of T and NK cells. …”
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Different immunological characteristics of asymptomatic and symptomatic COVID-19 patients without vaccination in the acute and convalescence stages by Li Li, Xin Zhang, Huimin Yan, Muwei Dai, Huixia Gao, Yuling Wang, Ping Jiang, Erhei Dai

“…Further analysis of the subsets of major immune cell types in COVID-19 patients with different clinical presentations in different stages showed that in the acute stages of disease progression, the T helper cell 1 (Th1), IgD+ B and neutrophil subsets increased in COVID-19 patients, especially in symptomatic patients, while the central memory CD4+T cells (CD4 TCM), mucosa-associated invariant T (MAIT) and NK cell subsets decreased significantly, especially in symptomatic patients. …”
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The Clinical Significance of HLA Compatibility Scores in Lung Transplantation by Liesbeth Daniëls, Liesbeth Daniëls, Hanne Beeckmans, Andrea Zajacova, Pieterjan Kerckhof, Saskia Bos, Maarten Naesens, Bart Vanaudenaerde, Frans Claas, Frans Claas, Robin Vos, Robin Vos

“…Since HLA disparity between donor and recipient plays a major role in rejection, we performed a single center, retrospective observational cohort analysis in our lung transplant cohort (n = 128) in which we calculated HLA compatibility scores for B-cell epitopes (HLAMatchmaker, HLA-EMMA), T-cell epitopes (PIRCHE-II) and missing self-induced NK cell activation (KIR Ligand Calculator). …”
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High-dose ascorbic acid synergizes with anti-PD1 therapy in non-small cell lung cancer in vitro and in vivo models by Hak Su Kim, Seung-hyun Kwon, Ok Kyung Choi, Taekyu Lim, Taekyu Lim

“…To investigate the molecular mechanism, tumor tissues from mice were analyzed by comprehensive proteomic profiling using nano-LC-ESI-MS/MS.ResultsPretreatment with a high dose of AA led to enhanced the sensitivity to the cytotoxicity of CD8+ T cells derived from healthy donor for H460 cells. Additionally, the combination of anti-PD1 and high-dose AA significantly increased CD8+ T cell cytotoxicity in H460 cells. …”
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Ruxolitinib and oHSV combination therapy increases CD4 T cell activity and germinal center B cell populations in murine sarcoma by Ravi Dhital, Yeaseul Kim, Doyeon Kim, Ilse Hernandez-Aguirre, Jack Hedberg, Alexia Martin, Kevin A. Cassady

“…To address these limitations, we developed a 46-color spectral flow cytometry panel for the detailed analysis of immune cell dynamics following repeated oHSV dosing. …”
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Development and Characterization of Anti-Nitr9 Antibodies by Radhika N. Shah, Ivan Rodriguez-Nunez, Donna D. Eason, Robert N. Haire, Julien Y. Bertrand, Valērie Wittamer, David Traver, Shila K. Nordone, Gary W. Litman, Jeffrey A. Yoder

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Regenerative fibroblasts derived from autologous skin tissue for the treatment of Sjögren’s syndrome: a case report by Zhao-Xia Ma, Xing-Fei Wu, Li Cao, Cheng-Yan Jiao, Dai-Ping Ma, Yun-Hui Zhao, Zhi-Xing Yang, Min Hu, Min Hu

“…ESR, B lymphocytes, rheumatoid factor IgM, and IgA declined, while the proportion of NK cells increased, and most of the cytokines returned to normal levels. …”
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Single‐cell landscape of the intrahepatic ecosystem in alcohol‐related liver disease by Xiaofang Zhao, Senyan Wang, Qi Liu, Wenjuan Wei, Xiaoyan Sun, Hao Song, Jing Xu, Shuijun Zhang, Hongyang Wang, Jing Fu

“…In this study, we performed single‐cell RNA sequencing on liver tissues obtained from ALD patients and healthy liver donors. We identified an ALB+KRT7+ epithelial population that expressed both hepatocyte and biliary markers significantly expanded in ALD livers. …”
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Downregulation of MerTK in circulating T cells of patients with non-proliferative diabetic retinopathy by Shimiao Bu, Shimiao Bu, Jiang-Yue Ling, Jiang-Yue Ling, Xiaojun Wu, Xiaojun Wu, Liting Zhang, Liting Zhang, Xiangyu Shi, Lang Huang, Zheng Zhao, Ying Yang, Zongqin Xiang, Yong U. Liu, Yong U. Liu, Yufeng Liu, Yuehong Zhang, Yuehong Zhang

“…This group included 12 healthy donors (HDs), 22 individuals with DM, and 26 with NPDR. …”
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