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2921
人纤溶酶原K5突变体在大肠杆菌中的优化表达
Published 2008-01-01“…通过电泳分析,获得mK5在原核系统pET22b-BL21的最优表达条件;组氨酸结合柱亲和层析、纯化获得mK5蛋白,SDS-PAGE和Western-blot方法鉴定mK5蛋白;MTT法分析mK5对人视网膜毛细血管内皮细胞(HRCEC)增殖的影响。【结果】 优化后的表达条件为:最适细菌培养温度为37℃,最佳抗生素浓度为50μg/mL羧苄青霉素,最适pH值为7.0,在A600为0.9~1.0时加入终浓度为1.0 mmol/L的IPTG为最佳诱导条件,37℃诱导10 h为最佳诱导时间;优化条件后mK5纯化蛋白得率为21 mg/L,产量较优化前(15 mg/L)提高近30%;mK5特异性地抑制HRCEC的增殖,IC50=40 nmol/L。…”
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2922
Trimethylamine-N-Oxide Promotes High-Glucose-Induced Dysfunction and NLRP3 Inflammasome Activation in Retinal Microvascular Endothelial Cells
Published 2023-01-01“…Human retinal microvascular endothelial cells (HRMECs) were treated for 72 h as follows: NG (normal glucose, D-glucose 5.5 mM), NG + TMAO (5 μM), HG (high glucose, D-glucose 30 mM), and HG + TMAO (5 μM). …”
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2923
Obraz starých Řeků v Carionově kronice. Příspěvek k dějinám recepce antiky
Published 2007-01-01“…This is the most transparent in his interpretation of the subjugation of Greece by Phillip the Macedon. This act is not seen as the end of old and famous freedom of Greece, as seen by Nauclerus and his source Justinus, but as the establishment of order between the fighting Greek towns. …”
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2924
Orphans in Mediterranean antiquity and early Christianity
Published 2016-06-01“…Part 3 surveys the recognition of orphans’ vulnerability in ancient Babylon, ancient Israel and early Judaism, ancient Greece, and imperial Rome. Part 4 discusses the treatment of orphans in early Christianity, focusing on the pre-Constantinian period. …”
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2925
One-Day Prediction of Biometeorological Conditions in a Mediterranean Urban Environment Using Artificial Neural Networks Modeling
Published 2013-01-01“…The present study, deals with the 24-hour prognosis of the outdoor biometeorological conditions in an urban monitoring site within the Greater Athens area, Greece. For this purpose, artificial neural networks (ANNs) modelling techniques are applied in order to predict the maximum and the minimum value of the physiologically equivalent temperature (PET) one day ahead as well as the persistence of the hours with extreme human biometeorological conditions. …”
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2926
Bassæ
Published 2013-03-01“…The temple of Apollo Epikourios – the helper – at Bassae, built in the fifth century BC, is located at an altitude of about 1130 metres on Mount Kotilio, in Arcadia, Peloponnese, Greece. In the mid-1980s, the temple was reinforced for reasons of preservation-restoration by earthquake-resistant scaffolding and covered by a gigantic canopy. …”
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2927
The concept of ‘smart cities’. Towards community development?
Published 2012-10-01“…Finally, in the last part, some conclusions and future prospects of the concept of ‘smart’ cities for community development in Greece are drawn.…”
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2928
Metadata: A pedagogical tool for the teaching of map projections in Elementary School
Published 2021-11-01“…The research was carried out in 17 Elementary Schools of Thessaloniki, Greece, where 6th-grade students (? = 655) were engaged in a series of activities related to metadata and map projections. …”
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2929
Development Education in European Rural Areas: The Work Done by NGODs
Published 2019-11-01“…Rural DEAR Agenda-EYD 2015 (Development Education in Rural Areas 2015) is a European project analysing DE initiatives by NGODs and CSOs in rural areas in seven European countries: Italy, Spain, Greece, Poland, Bulgaria, Cyprus, and Malta. A questionnaire was designed and sent to these NGODs/CSOs. …”
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2930
Entre information et propagande : la Grèce dans la presse britannique et française pendant la Première Guerre mondiale
Published 2006-09-01“…Next, we describe the image of Greece given through the English and French press during the First World War, specifying the events which the newspapers particularly concentrated on. …”
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2931
Live Broadcasting of High Definition Audiovisual Content Using HDTV over Broadband IP Networks
Published 2008-01-01“…The implemented system was successfully applied in real-world applications, such as the transmission of cultural events from Thessaloniki Concert Hall throughout Greece as well as the reproduction of Philadelphia Orchestra performances (USA) via Internet2 and GEANT backbones.…”
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2932
The Spatial Extensions of the Right to Seek Asylum
Published 2021-02-01“…The site of exploration is the Eastern Mediterranean Route (EMR), a passage expanding from Greece to Germany through the Balkans, used for many years as an entry path into Europe. …”
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2933
Effect of Rosa Mosqueta Husk Extract (Rosa rubiginosa) on Thermooxidation of Grape Seed Oil
Published 2019-01-01“…The induction period (OSI), together with the formation of polar compounds (CP) by HPSEC and the retention of alpha-tocopherol (AT) by HPLC, was the main parameter studied during this deterioration process. …”
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2934
27th Congress of SCTM
Published 2024-12-01“…It is particularly important to mention the wide range of oral and poster presentations by researchers representing Austria, Azerbaijan, Bosnia and Herzegovina, Bulgaria, Croatia, Germany, Greece, Italy, Kosovo, Montenegro, Poland, Serbia, Slovenia, Spain, Romania and Russia. …”
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2935
Cinquante ans depuis la révolution des œillets
Published 2024-07-01“…The article seeks to give a synthetic account of the complex panorama that marked a process that, together with the end of the “dictatorship of the colonels” in Greece and Spanish democratization, conditioned two of the great milestones of the last quarter of the 20th century: the emergence of the European Union and the neoliberal wave.…”
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2936
Deucalion et les os de la mère
Published 2022-04-01“…Through a path mixing art, architecture and literature, going from China to the United States as well as Greece and Italy, this article weaves threads and links between periods and techniques that may seem quite far apart. …”
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2937
Investigation of the Geomagnetically Induced Current Index Levels in the Mediterranean Region During the Strongest Magnetic Storms of Solar Cycle 24
Published 2023-02-01“…Here, we exploit data from the HellENIc GeoMagnetic Array, which is deployed in Greece, complemented by magnetic observatories in the Mediterranean region (Italy, France, Spain, Algeria, and Turkey), to calculate values of the GIC index, that is, a proxy of the geoelectric field calculated entirely from geomagnetic field variations. …”
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2938
Effect of Wheat Replacement by Pulse Flours on the Texture, Color, and Sensorial Characteristics of Crackers: Flash Profile Analysis
Published 2022-01-01“…Flash profile analysis was conducted in Greece and Indonesia in order to allow culture comparison of the profiling of the samples and language by the subjects of the panel. …”
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2939
From Savage to Sublime (And Partway Back): Indians and Antiquity in Early Nineteenth-Century American Literature
Published 2016-06-01“…Bringing together these two quite different worlds, however, resulted in various ambiguities: it simultaneously reinforced the suggestion that Indians were already part of the past (providing a certain complicity with the continuing destruction of Indian culture) and questioned the then dominant image of Ancient Greece and Rome as examples of some of the highest levels attainable of government and human civilization.…”
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2940
Saliamonas Antanaitis (1894–1973) and his research into the old mathematics
Published 2002-12-01“…Another one was an original book about a scientist of Ancient Greece Archimedes (287 BC–212 BC). S. Antanaitis having made a thorough analysis of works of European science historians presented these science personalities and underlined importance of their works for the development of the higher mathematics. …”
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