Use of murine model to study virulence of epidemically significant Mycobacterium tuberculosis genotypes and to evaluate effectiveness of chemotherapy by Dr Igor Mokrousov, Prof. Tatiana Vinogradova, Dr. Marine Dogonadze, Dr. Natalia Zabolotnykh, Dr. Marina Dyakova, Dr. Dilyara Esmedlyaeva, Dr. Maria Vitovskaya, Dr Olga Rubtsova, Dr Sergei Chekrygin, Dr Anna Vyazovaya, Prof. Boris Ariel

“…Using the murine model, we compared virulence of M. tuberculosis strains of the medically significant genotypes and evaluated the effectiveness of anti-TB therapy and inflammatory response in infected mice. …”
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Characterization and Expression Profiling of Orphan Genes in Rapeseed (<i>Brassica napus</i>) Provide Insights into Tissue Development and Cold Stress Adaptation by Hong Lang, Yuting Zhang, Baofeng Wang, Kexin Li, Mingliang Jiang

“…This study aims to identify and characterize OGs in rapeseed to evaluate their association with stress adaptation and lineage-specific traits. …”
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A protocol for organoids from the gynecomastia patients by Fangjian Shang, Zihao Li, Ji Feng, Qi Wang, Mengyang An, Zengren Zhao, Bo Liu

“…Organoid fidelity was evaluated using hematoxylin and eosin (H&amp;E) staining, immunohistochemistry (IHC), immunofluorescence (IF), and quantitative PCR (qPCR), targeting a panel of lineage-specific and proliferative markers.ResultsThe organoids recapitulated key histological and molecular features of their corresponding source tissues, including epithelial architecture and expression of CK14, CK18, Ki67, and ERα. …”
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Patient-specific HLA-I subtypes predict response to immune checkpoint blockade by Kyrillus S. Shohdy, Jack Atherton, Jessica Longland, Jennifer Alison, Manon Pillai, Fiona Thistlethwaite

“…A02:01 showed a tumor lineage-specific distribution. One hundred and forty patients received ICB and had evaluable response status. …”
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Análisis inmunofenotípico de muestras normales de médula ósea: aplicaciones en el control de calidad en los laboratorios de citometría by Dian Carolina Roa-Higuera, Susana Fiorentino, Viviana Marcela Rodriguez-Pardo, Alba Myriam Campos-Arenas, Elvira Antonia Infante-Acosta, Claudia Cecilia Cardozo-Romero, Sandra Milena Quijano-Gómez

“…To describe a standardized flow cytometry protocol for the relative and absolute quantification of hematopoietic cell subpopulationsfrom normal bone marrow, and to evaluate the expression of different lineage-specific cell markers with a reactivity associated to celldifferentiation to be used as part of the routine quality control in cytometry laboratories. …”
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Epigenetic regulation during fetal femur development: DNA methylation matters. by María C de Andrés, Emmajayne Kingham, Kei Imagawa, Antonio Gonzalez, Helmtrud I Roach, David I Wilson, Richard O C Oreffo

“…Methylated cytosine residues at CpG dinucleotides are commonly associated with gene repression; conversely, strategic loss of methylation during development could lead to activation of lineage-specific genes. Evidence is emerging that bone development and growth are programmed; although, interestingly, bone is constantly remodelled throughout life. …”
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Effects of Serial Passage on the Characteristics and Cardiac and Neural Differentiation of Human Umbilical Cord Wharton’s Jelly-Derived Mesenchymal Stem Cells by Jianchun Lian, Shijie Lv, Chang Liu, Yang Liu, Shujun Wang, Xin Guo, Feng Nan, Hua Yu, Xin He, Guangwei Sun, Xiaojun Ma

“…Serial passage is the main approach to expand stem cells. This study evaluated effects of serial passage on the biological characteristics of human umbilical cord Wharton’s jelly-derived MSCs (WJ MSCs). …”
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Three-Dimensional Trilineage Differentiation Conditions for Human Induced Pluripotent Stem Cells by Md Sharifur Rahman, Guangyan Qi, Quan Li, Xuming Liu, Jianfa Bai, Mingshun Chen, Anthony Atala, Xiuzhi Susan Sun

“…Differentiated spheroids exhibited distinct morphological characteristics and significantly increased expression of key lineage-specific markers—<i>FOXA2</i> (endoderm), <i>Brachyury</i> (mesoderm), and <i>PAX6</i> (ectoderm)—compared to undifferentiated controls. …”
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Hematopoietic stem cell discovery: unveiling the historical and future perspective of colony-forming units assay by Nur Afizah Yusoff, Zariyantey Abd Hamid, Siti Balkis Budin, Izatus Shima Taib

“…They are also able to differentiate into lineage-specific cell types that are specialized and matured. …”
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Knockdown of IER3 Promotes Osteogenic Differentiation of Human Mesenchymal Stem Cells by Yuqing Han, Hongyang Ma, Zhihui Tang, Chanyuan Jin

“…Then, stable IER3–knockdown hMSCs were generated using IER3–targeted shRNA lentiviral vectors, and the impact of IER3 on osteogenic differentiation was evaluated through both in vitro cell induction and hMSCs subcutaneous implantation model of nude mice. …”
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Regulating mitochondrial oxidative phosphorylation and MAPK signaling: wedelolactone as a novel therapeutic for radiation-induced thrombocytopenia by Zhichao Li, Qinyao Li, Shuang Wu, Xinyue Mei, Xiao Qi, Sheng Liu, Gan Qiao, Hongping Shen, Jiesi Luo, Jing Zeng, Feihong Huang, Rong Li, Rong Li, Long Wang

“…This study investigates wedelolactone (WED), a metabolite of a botanical drug, as a potential treatment for RIT.MethodsIn vitro experiments were conducted using Meg‐01 and K562 cell lines to evaluate the effects of WED on megakaryocyte differentiation and maturation. …”
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Development of a Quadruplex RT-qPCR Assay for Rapid Detection and Differentiation of PRRSV-2 and Its Predominant Genetic Sublineages in China by Guishan Ye, Siyu Xiong, Zhipeng Su, Guosheng Chen, Siyuan Liu, Zixuan Wang, Huanchun Chen, Anding Zhang

“…The method’s performance was evaluated in terms of specificity, sensitivity, repeatability, stability, limit of detection (LOD), and consistency with sequencing results. …”
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Small molecules direct the generation of ameloblast-like cells from human embryonic stem cells by Ximei Zhu, YiMeng Zhao, Xiaofan Bai, Qiannan Dong, Chunli Tian, Ruilin Sun, Congjuan Yan, Jianping Ruan, Zhongbo Liu, Jianghong Gao

“…Quantitative reverse transcription polymerase chain reaction (qRT-PCR) and immunofluorescence were conducted to assess the expression of lineage-specific markers. Alizarin Red S (ARS) staining was performed to evaluate the formation of mineralization nodules. …”
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Phenotypic and genotypic characterization of Mycobacterium tuberculosis pyrazinamide resistance—India, 2018–2020 by Sembulingam Tamilzhalagan, Evanslin Santus Justin, Ashok Selvaraj, Karthick Venkateswaran, Arun Kumar Sivakumar, Suganthi Chittibabu, Heather P. McLaughlin, Patrick K. Moonan, Jonathan P. Smith, Sakthi Suba, Mukesh Kumar Sathya Narayanan, Christine S. Ho, Nishant Kumar, Srikanth P. Tripathy, Srikanth P. Tripathy, Siva K. Shanmugam, Patricia J. Hall-Eidson, Uma Devi Ranganathan

“…This nationwide study was conducted to explore the prevalence of mutations conferring PZA resistance, catalogue mutation diversity, investigate the associations of PZA resistance with specific lineages, examine co-resistance to 13 first- and second-line drugs, and evaluate the diagnostic accuracy of sequencing pncA and panD genes for predicting PZA resistance. …”
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Enhanced rotator cuff tendon-bone interface regeneration with injectable manganese-based mesoporous silica nanoparticle-loaded dual crosslinked hydrogels by Zihang Chen, Youjie Liu, Tianxiang Liang, Zhaoyuan Du, Liming Deng, Zhiwen Wu, Ye Li, Haobo Zhong, JinJin Ma, Riwang Li, Huajun Wang, Qiu Dong, Tao Yu, Xiaofei Zheng

“…The ion release profiles of Si4+ and Mn4+ were evaluated to assess sustained delivery. Rheological properties and self-healing capabilities were examined to determine injectability and in vivo stability. …”
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Improved ChIP Sequencing for H3K27ac Profiling and Super-Enhancer Analysis Assisted by Fluorescence-Activated Sorting of Formalin-Fixed Paraffin-Embedded Tissues by Nenggang Jiang, Zhihao Wen, Huan Tao, Hongyan Liao

“…In addition, we also evaluated the protocol using cultured pure cell lines, and the results indicated that the sequencing data obtained through this protocol had high fidelity and reproducibility. …”
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Genomic insights into biofilm-associated virulence in extensively drug-resistant Acinetobacter baumannii by Made Rai Dwitya Wiradiputra, Piyatip Khuntayaporn, Krit Thirapanmethee, Pagakrong Wanapaisan, Mullika Traidej Chomnawang

“…All ST2 strains carried bap type-2 with 4–11 BC repeats, while ST25 harboured bap type-3. ompA variants also showed lineage specificity (variant V1 in ST2 and V3 in ST25). …”
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