Genes and light: many years later by A. O. Ruvinsky

“…Georgian white mutation likely causes a low expression of KIT gene. Presented here considerations of KIT protein and its ligand KITLG molecular and cellular interactions suggest a possibility for slow development of W G/W G blastocysts; hence many such blastocysts might be late and miss an opportunity for successful implantation. …”
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LIMD1-AS1 promotes the progression of prostate cancer and affects the function of prostate cancer cells by down-regulating miR-29c-3p by Yongsheng Yu, Nan He, Zhaolu Song, Chang Wang, Jinhuang Xu

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Large-scale Cosmic-ray Anisotropies with 19 yr of Data from the Pierre Auger Observatory by A. Abdul Halim, P. Abreu, M. Aglietta, I. Allekotte, K. Almeida Cheminant, A. Almela, R. Aloisio, J. Alvarez-Muñiz, A. Ambrosone, J. Ammerman Yebra, G. A. Anastasi, L. Anchordoqui, B. Andrada, L. Andrade Dourado, S. Andringa, L. Apollonio, C. Aramo, P. R. Araújo Ferreira, E. Arnone, J. C. Arteaga Velázquez, P. Assis, G. Avila, E. Avocone, A. Bakalova, F. Barbato, A. Bartz Mocellin, J. A. Bellido, C. Berat, M. E. Bertaina, G. Bhatta, M. Bianciotto, P. L. Biermann, V. Binet, K. Bismark, T. Bister, J. Biteau, J. Blazek, C. Bleve, J. Blümer, M. Boháčová, D. Boncioli, C. Bonifazi, L. Bonneau Arbeletche, N. Borodai, J. Brack, P. G. Brichetto Orchera, F. L. Briechle, A. Bueno, S. Buitink, M. Buscemi, M. Büsken, A. Bwembya, K. S. Caballero-Mora, S. Cabana-Freire, L. Caccianiga, F. Campuzano, R. Caruso, A. Castellina, F. Catalani, G. Cataldi, L. Cazon, M. Cerda, B. Čermáková, A. Cermenati, J. A. Chinellato, J. Chudoba, L. Chytka, R. W. Clay, A. C. Cobos Cerutti, R. Colalillo, R. Conceição, A. Condorelli, G. Consolati, M. Conte, F. Convenga, D. Correia dos Santos, P. J. Costa, C. E. Covault, M. Cristinziani, C. S. Cruz Sanchez, S. Dasso, K. Daumiller, B. R. Dawson, R. M. de Almeida, B. de Errico, J. de Jesús, S. J. de Jong, J. R. T. de Mello Neto, I. De Mitri, J. de Oliveira, D. de Oliveira Franco, F. de Palma, V. de Souza, E. De Vito, A. Del Popolo, O. Deligny, N. Denner, L. Deval, A. di Matteo, C. Dobrigkeit, J. C. D’Olivo, L. M. Domingues Mendes, Q. Dorosti, J. C. dos Anjos, R. C. dos Anjos, J. Ebr, F. Ellwanger, M. Emam, R. Engel, I. Epicoco, M. Erdmann, A. Etchegoyen, C. Evoli, H. Falcke, G. Farrar, A. C. Fauth, T. Fehler, F. Feldbusch, A. Fernandes, B. Fick, J. M. Figueira, P. Filip, A. Filipčič, T. Fitoussi, B. Flaggs, T. Fodran, M. Freitas, T. Fujii, A. Fuster, C. Galea, B. García, C. Gaudu, P. L. Ghia, U. Giaccari, F. Gobbi, F. Gollan, G. Golup, M. Gómez Berisso, P. F. Gómez Vitale, J. P. Gongora, J. M. González, N. González, D. Góra, A. Gorgi, M. Gottowik, F. Guarino, G. P. Guedes, E. Guido, L. Gülzow, S. Hahn, P. Hamal, M. R. Hampel, P. Hansen, V. M. Harvey, A. Haungs, T. Hebbeker, C. Hojvat, J. R. Hörandel, P. Horvath, M. Hrabovský, T. Huege, A. Insolia, P. G. Isar, P. Janecek, V. Jilek, J. Jurysek, K.-H. Kampert, B. Keilhauer, A. Khakurdikar, V. V. Kizakke Covilakam, H. O. Klages, M. Kleifges, F. Knapp, J. Köhler, F. Krieger, M. Kubatova, N. Kunka, B. L. Lago, N. Langner, M. A. Leigui de Oliveira, Y. Lema-Capeans, A. Letessier-Selvon, I. Lhenry-Yvon, L. Lopes, J. P. Lundquist, A. Machado Payeras, D. Mandat, B. C. Manning, P. Mantsch, F. M. Mariani, A. G. Mariazzi, I. C. Mariş, G. Marsella, D. Martello, S. Martinelli, O. Martínez Bravo, M. A. Martins, H.-J. Mathes, J. Matthews, G. Matthiae, E. Mayotte, S. Mayotte, P. O. Mazur, G. Medina-Tanco, J. Meinert, D. Melo, A. Menshikov, C. Merx, S. Michal, M. I. Micheletti, L. Miramonti, S. Mollerach, F. Montanet, L. Morejon, K. Mulrey, R. Mussa, W. M. Namasaka, S. Negi, L. Nellen, K. Nguyen, G. Nicora, M. Niechciol, D. Nitz, D. Nosek, V. Novotny, L. Nožka, A. Nucita, L. A. Núñez, C. Oliveira, M. Palatka, J. Pallotta, S. Panja, G. Parente, T. Paulsen, J. Pawlowsky, M. Pech, J. Pȩkala, R. Pelayo, V. Pelgrims, L. A. S. Pereira, E. E. Pereira Martins, C. Pérez Bertolli, L. Perrone, S. Petrera, C. Petrucci, T. Pierog, M. Pimenta, M. Platino, B. Pont, M. Pothast, M. Pourmohammad Shahvar, P. Privitera, M. Prouza, S. Querchfeld, J. Rautenberg, D. Ravignani, J. V. Reginatto Akim, A. Reuzki, J. Ridky, F. Riehn, M. Risse, V. Rizi, E. Rodriguez, J. Rodriguez Rojo, M. J. Roncoroni, S. Rossoni, M. Roth, E. Roulet, A. C. Rovero, A. Saftoiu, M. Saharan, F. Salamida, H. Salazar, G. Salina, P. Sampathkumar, J. D. Sanabria Gomez, F. Sánchez, E. M. Santos, E. Santos, F. Sarazin, R. Sarmento, R. Sato, C. M. Schäfer, V. Scherini, H. Schieler, M. Schimassek, M. Schimp, D. Schmidt, O. Scholten, H. Schoorlemmer, P. Schovánek, F. G. Schröder, J. Schulte, T. Schulz, S. J. Sciutto, M. Scornavacche, A. Sedoski, A. Segreto, S. Sehgal, S. U. Shivashankara, G. Sigl, K. Simkova, F. Simon, R. Šmída, P. Sommers, R. Squartini, M. Stadelmaier, S. Stanič, J. Stasielak, P. Stassi, S. Strähnz, M. Straub, T. Suomijärvi, A. D. Supanitsky, Z. Svozilikova, Z. Szadkowski, F. Tairli, A. Tapia, C. Taricco, C. Timmermans, O. Tkachenko, P. Tobiska, C. J. Todero Peixoto, B. Tomé, Z. Torrès, A. Travaini, P. Travnicek, M. Tueros, M. Unger, R. Uzeiroska, L. Vaclavek, M. Vacula, J. F. Valdés Galicia, L. Valore, E. Varela, V. Vašíčková, A. Vásquez-Ramírez, D. Veberič, I. D. Vergara Quispe, V. Verzi, J. Vicha, J. Vink, S. Vorobiov, C. Watanabe, A. A. Watson, A. Weindl, M. Weitz, L. Wiencke, H. Wilczyński, D. Wittkowski, B. Wundheiler, B. Yue, A. Yushkov, O. Zapparrata, E. Zas, D. Zavrtanik, M. Zavrtanik, The Pierre Auger Collaboration

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Knockdown MTDH Inhibits Glioma Proliferation and Migration and Promotes Apoptosis by Downregulating MYBL2 by Junqi Fu, Jun Peng, Guolong Tu

“…Cell counting kit 8 (CCK-8) was used to detect cell proliferation, and flow cytometry was applied to measure cell apoptosis. …”
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Downregulation of LncRNA SNHG7 Sensitizes Colorectal Cancer Cells to Resist Anlotinib by Regulating miR-181a-5p/GATA6 by Deng Pan, Kehe Chen, Ping Chen, Yu Liu, Yingying Wu, Jingning Huang

“…We performed the Cell Counting Kit-8 and Colony formation assay, flow cytometric analysis, RNA pull-down, RNA-binding protein immunoprecipitation assay, and Luciferase reporter assay to confirm the interaction among SNHG7, miR-181a-5p, and GATA6. …”
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lncRNA FR215775 Regulates Th2 Differentiation in Murine Allergic Rhinitis by Yue Ma, Le Shi, Keqing Zhao, Chunquan Zheng

“…To identify the effect of long noncoding RNA (lncRNA) FR215775 in regulating CD4+ T cells on murine models of allergic rhinitis (AR), the expression of lncRNA FR215775 in primary Th2 cells was detected through qRT-PCR. After knocking down the expression of lncRNA FR215775 via Sh-FR215775-Ads, Cell Counting Kit-8, cytometric bead array, and fluorescence-activated cell sorting were performed to determine its functions in vitro. …”
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LINC02389/miR-7-5p Regulated Cisplatin Resistance of Non-Small-Cell Lung Cancer via Promoting Oxidative Stress by Peng Ma, Wen Han, Cunying Meng, Xiaohong Tan, Pengfei Liu, Lei Dong

“…The interaction between LINC02389 and miR-7-5p was verified by dual luciferase reporter gene assay, RNA pull-down assay, and RNA immunoprecipitation (RIP) assay. …”
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CircTRRAP Knockdown Has Cardioprotective Function in Cardiomyocytes via the Signal Regulation of miR-370-3p/PAWR Axis by Yuan Zhang, Zhenggong Li, Jiao Wang, Hao Chen, Rui He, Hongkun Wu

“…Oxidative stress was assessed by the commercial kits. Dual-luciferase reporter assay, RNA immunoprecipitation, and RNA pull-down assays were performed for the validation of target interaction. …”
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甲基转移酶3对人脑血管平滑肌细胞基因表达及增殖和迁移功能的影响 Effects of Methyltransferase 3 on Gene Expression, Proliferation, and Migration Functions of Human Brain Vascular Smooth Muscle Cells... by 孟晨曦1，孙洪英2，张佳2，毛戬2，杨阳3，策乐木格3 (MENG Chenxi1, SUN Hongying2, ZHANG Jia2, MAO Jian2, YANG Yang3, Celemuge3 )

“…Conclusions Interfering with METTL3 expression may promote the proliferation and migration abilities of HBVSMC by down-regulating the m6A modification levels of ZNF395 and ZNF548 mRNA. …”
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HSP60 inhibits DF-1 apoptosis through its mitochondrial signal peptide by Shengliang Cao, Yanlan Li, Lele Chen, Xiaojing Lei, Xiujuan Feng, Yubao Li

“…First, the CRISPR-Cas9 system was employed to establish the HSP60 knockout DF-1 cell line (DF-1-HSP60-KO), and the apoptosis level of DF-1-HSP60-KO cell line was assessed by flow cytometry and ELISA apoptosis kit. Then, the effect of knockdout of HSP60 on relevant apoptotic factors was assessed by Western blotting and RT-PCR analysis. …”
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circ-SIRT1 Promotes Colorectal Cancer Proliferation and EMT by Recruiting and Binding to eIF4A3 by Xiangjie Wang, Shuang Liu, Bin Xu, Yabin Liu, Peng Kong, Changlin Li, Binghui Li

“…Colony formation and Cell Counting Kit-8 assays were used to evaluate the effect of circ-SIRT1 knockdown on the proliferative ability of CRC cells. …”
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Inhibition of miR-182-5p Targets FGF9 to Alleviate Osteoarthritis by Yang Sun, Sanmao Su, Mengjun Li, Ang Deng

“…The protein FGF9 was tested by western blotting. Cell counting kit-8 (CCK8), plate cloning assay, and flow cytometry were conducted to evaluate cell proliferation and apoptosis. …”
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CircBAZ1B stimulates myocardial ischemia/reperfusion injury (MI/RI) by modulating miR-1252-5p/ATF3-mediated ferroptosis by Ruili Wei, Tianxiao Yang, Weihong Li, Xiqian Wang

“…RNA pull-down, RNA immunoprecipitation (RIP), and a dual-luciferase reporter system were utilized for verification of the molecular interactions. …”
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Dysfunction in mitochondrial electron transport chain drives the pathogenesis of pulmonary arterial hypertension: insights from a multi-omics investigation by Xin Zhang, Jieling Li, Minyi Fu, Xijie Geng, Junjie Hu, Ke-Jing Tang, Pan Chen, Jianyong Zou, Xiaoman Liu, Bo Zeng

“…Mitochondria-related genes highly correlated with these metabolites included KIT, OTC, CAMK2A, and CHRNA1. Conclusions Down-regulation of electron transport from cytochrome c to oxygen in mitochondrial ETC and disruption of the citric acid cycle homeostasis may contribute to PAH pathogenesis. 3-phenyllactic acid emerges as a potential novel diagnostic biomarker for PAH. …”
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miRNA-541-5p regulates myocardial ischemia–reperfusion injury by targeting ferroptosis by ZhiYu Zhao, BoXia Li, DianWei Cheng, YuFang Leng

“…Methods SD rats and H9C2 cells were used as subjects. ELISA kits quantified MDA, SOD, GSH, LDH, and ferritin levels. …”
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Systematic Review and Meta-Analysis of the Y-Balance Test Lower Quarter: Reliability, Discriminant Validity, and Predictive Validity by Phillip Plisky, Katherine Schwartkopf-Phifer, Bethany Huebner, Mary Beth Garner, Garrett Bullock

“…Studies were excluded if the Y-Balance Test kit was not utilized during testing or if there was a major deviation from the Y-Balance test procedure. …”
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Effect of Whole Wheat Noodles on Biochemical Indexes and Histopathology of Type 2 Diabetic Rats by Li WU, Yuan ZHANG, Chunhong TANG, Huan YOU, Haijun CHANG, Shuai ZHANG

“…Fasting glucose value was measured by the tail blood sampling method, and the kit was used to measure the blood lipid biochemical indexes and serum oxidative stress levels. …”
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Bone Defect Repair Using a Bone Substitute Supported by Mesenchymal Stem Cells Derived from the Umbilical Cord by Michal Kosinski, Anna Figiel-Dabrowska, Wioletta Lech, Lukasz Wieprzowski, Ryszard Strzalkowski, Damian Strzemecki, Lukasz Cheda, Jacek Lenart, Krystyna Domanska-Janik, Anna Sarnowska

“…Sometimes the regeneration process of bone loss is impaired, significantly slowed down, or does not occur, e.g., in congenital defects. …”
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Upregulated SAE1 Drives Tumorigenesis and Is Associated with Poor Clinical Outcomes in Breast Cancer by Hong Liu, Jing Wang, Yunhai Li, Feng Luo, Lei Xing

“…Western blots confirmed that the expression of PI3K/Akt/mTOR pathway-related proteins (p-PI3K, p-AKT, and mTOR) in BC cells was decreased after knocking down SAE1. Conclusion. SAE1 was highly expressed in BC. …”
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gga-miR-6634-5p Affects the proliferation and steroid hormone secretion of chicken (Gallus Gallus) granulosa cells by targeting MMP16 by Yixiang Tian, Xing Wu, Zihao Zhang, Yijie Li, Meng Hou, Ruirui Jiang, Donghua Li, Yanhua Zhang, Yulong Guo, Guoxi Li, Xiaojun Liu, Xiangtao Kang, Yadong Tian, Wenting Li, Yuetao Li

“…We found that gga-miR-6634-5p significantly down-regulated the mRNA levels of proliferation-related genes (CCND1, CDK1, and CDK6), decreased cell viability, the number of EdU-labelled positive cells, and the percentage of S-phase cells, as analysed by quantitative real time PCR (qRT-PCR), cell counting kit-8 (CCK-8), 5-ethynyl-2′deoxyuridine (EdU) and flow cytometry analyses (P < 0.01 or P < 0.05). qRT-PCR and enzyme-linked immunosorbent assay (ELISA) results demonstrated that gga-miR-6634-5p up-regulated the expression of steroid synthesis-related genes (CYP19A1, 3β-HSD, StAR and FSHR) (P < 0.01 or P < 0.05), as well as the secretion of estradiol (E2) and progesterone (P4) (P < 0.01 or P < 0.05). …”
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