Mechanism of Inhibition of Inflammation in Osteoarthritis by Tougu Xiaotong Capsule via Regulating Ras-Raf-MEK1/2-ERK1/2 Signaling Pathway by Munan LIN, Xiang SHAO, Limei XU, Xin ZHANG, Zaishi ZHU, Yidan HAN, Xinwei DUAN, Jianmei CHEN, song GAO, Xihai LI

“…Isolated chondrocytes of passage two were divided into blank group, model group(treatment with 10 ng/mL of LPS for 8 h), inhibitor group(treatment with 10 ng/mL of U0126 for 30 min followed by 10 ng/mL of LPS for additional 8 h)and TXC group(treatment with 10 ng/mL of LPS plus 300 μg/mL of TXC for 8 h).Western blot was carried out to detect the expression of Ras, Raf, p-ERK1/2, MEK1/2, MMP-3 and MMP-13.qPCR was used to detect the mRNAs expression of MEK1/2, ERK1/2, MMP-3 and MMP-13, as well as the expression of miR-27b, miR-34a and miR-146a.Results:1)The cytoplasm of isolated cells(P2)from the cartilage of knee joint of 4-week-old SD male rats was stained brown by typeⅡcollagen immunohistochemical staining as compared with the negative control cells, which indicated that the extracted cells were chondrocytes.2)The expression of Ras, Raf, MEK1/2, MMP-3, MMP-13 in the model group was significantly higher than that in the blank group, respectively(<italic>P</italic>&lt;0.01, <italic>P</italic>&lt;0.05), but the expression of p-ERK1/2 had no significant difference(<italic>P</italic>&gt;0.05);the expression of Ras, Raf, p-ERK1/2, MEK1/2, MMP-3, MMP-13 in both the inhibitor group and the TXC group was lower than that in the model group, respectively(<italic>P</italic>&lt;0.01, <italic>P</italic>&lt;0.05), but the expression of MEK1/2 in the TXC group had no significant difference compared to that in the model group(<italic>P</italic>&gt;0.05).3)The mRNA expression of MEK1/2, ERK1/2, MMP-3 and MMP-13 in the model group was significantly higher than that in the blank group, respectively(<italic>P</italic>&lt;0.01);their expression in both the inhibitor group and the TXC group was lower than that in the model group, respectively(<italic>P</italic>&lt;0.01, <italic>P</italic>&lt;0.05).4)The expression of miR-27b in the model group was significantly lower than that in the blank group(<italic>P</italic>&lt;0.01), but its expre-ssion in both the inhibitor group and the TXC group was higher than that in the model group(<italic>P</italic>&lt;0.01);the expression of both miR-34a and miR-146a in the model group was significantly higher than that in the blank group, respectively(<italic>P</italic>&lt;0.01, <italic>P</italic>&lt;0.05);however, their expression in both the inhibitor group and the TXC group was lower than that in the model group, respectively(<italic>P</italic>&lt;0.01, <italic>P</italic>&lt;0.05).Conclusion:Tougu Xiaotong capsule regulates the expression of key factors in Ras-Raf-MEK1/2-ERK1/2 signaling pathway and miR-27b, miR-34a and miR-146a to regulate the inflammatory of chondrocytes, thereby inhibiting osteoarthritis inflammation and protecting articular cartilage.…”
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Efficacy of tranilast in preventing exacerbating cardiac function and death from heart failure in muscular dystrophy patients with advanced-stage heart failure: a single-arm, open-... by Tsuyoshi Matsumura, Takayasu Fukudome, Yasufumi Motoyoshi, Akinori Nakamura, Satoshi Kuru, Kazuhiko Segawa, Ruriko Kitao, Chigusa Watanabe, Takuhisa Tamura, Toshiaki Takahashi, Hiroya Hashimoto, Masahiro Sekimizu, Akiko M. Saito, Masanori Asakura, Koichi Kimura, Yuko Iwata

“…All 15 patients who completed the short-term treatment consented to be enrolled in long-term therapy for an additional 116 weeks. After all participants completed the long-term treatment, we analyzed all data. …”
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Multiple ovulation was positively associated with milk yield independently of circulating progesterone concentrations in multiparous high-producing Holstein cows submitted to Doubl... by T.J. Valdés-Arciniega, E. Anta-Galván, I.M.R. Leão, T.O. Cunha, M.S. El Azzi, N.B. Cook, J.P.N. Martins

“…In summary, MOV in multiparous high-producing Holstein cows was highly associated with serum P4 concentrations, milk yield, and previous MOV. In addition, an increased proportion of MOV was associated with high milk yield tertile (≥58.5 kg/d) regardless of the serum P4 concentrations during the growth of the ovulatory follicle.…”
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The use of ambon banana (Musa paradisiaca var. sapientum) stems flour in grouper (Epinephelus lanceolatus ♂× Epinephelus fuscoguttatus ♀) floating net cage nursery by Dinamella Wahjuningrum, Diah Ayu Lestari, Aldy Mulyadin, Irzal Effendi

“…On the 14th day of the hematological test, it was known that the highest total erythrocyte, hemoglobin, hematocrit, and total leukocyte was found in treatment B, however, the differential leukocyte did not differ significantly (P>0.05). The addition of Ambon banana stems flour in feed in the first 14 days (B) can improve health status but has not been able to increase production performance and morphometric grouper.   …”
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SSL5-AnxA5 Fusion Protein Constructed Based on Human Atherosclerotic Plaque scRNA-Seq Data Preventing the Binding of Apoptotic Endothelial Cells, Platelets, and Inflammatory Cells by Yifei Zhao, Xingyu He, Teng Hu, Tianli Xia, Fangyang Huang, Changming Li, Yiming Li, Fei Chen, Mao Chen, Jun Ma, Yong Peng

“…SSL5-AnxA5 effectively inhibited the combination of endothelial cells with inflammatory cells and the interaction of activated platelets with inflammatory cells. Additionally, this fusion protein exhibited remarkable capability in binding to apoptotic cells. …”
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Hubungan Electronic Customer Relationship Management (E-Crm) Terhadap Loyalitas Pasien Di Rumah Sakit: Tinjauan Pustaka by Sabrina Aulia Rahma, Holipah Holipah, Harun Al Rasyid, Fida Rahmayanti

“…Selain itu faktor keberhasilan penerapan E-CRM sebagai alat untuk menjaga dan meningkatkan loyalitas pasien adalah faktor manusia, diharapkan petugas medis memiliki sikap melayani yang baik, sopan, adil dan humanis.   …”
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Current Enzooticity of <i>Dirofilaria immitis</i> and <i>Angiostrongylus vasorum</i> in Central and Southern Italy by Donato Traversa, Simone Morelli, Angela Di Cesare, Chiara Astuti, Alessandra Barlaam, Mariasole Colombo, Fabrizia Veronesi, Barbara Paoletti, Raffaella Iorio, Raffaella Maggi, Alessandra Passarelli, Alessia Pede, Linda Rossi, Manuela Diaferia

“…Indeed, although some dogs positive for <i>D. immitis</i> had a history of travel in enzootic areas, the majority of them were never moved, indicating that they acquired the parasite in the region where they live. Additionally, <i>A. vasorum</i> is stably enzootic in the study areas, as also are other extraintestinal nematodes (i.e., <i>D. repens</i> and <i>C. aerophila</i>) that are more frequently detected today than in the past. …”
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Antioxidant, Physiochemical, and Sensory Properties of Functional Marshmallow Produced from Honey, Strawberry Concentrates, and Hibiscus Extract by Maher M. Al-Dabbas, Etaf G. Abu Samaan, Sehar Iqbal, Hani J. Hamad, Rawan Al-Jaloudi, Mohammad Shahein, Bha’a Aldin Al-Nawasrah, Abdalrahman Al-Zabt, Doa`a Al-Refaie, Nisreen Shehadeh, Mahmoud Abughoush

“…Among the formulations, F5 exhibited the highest phenolic and flavonoids contents (89.8 mg GAE/100 g and 1.62 mg RE/100 g, respectively) and reducing power activity (197.8% equivalent to 30 µg vitamin C). Additionally, F3 and F4 showed the strongest DPPH scavenging activity, with IC₅₀ values of 8.0 and 5.1 mg/mL, respectively. …”
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Single-cell profiling reveals a reduced epithelial defense system, decreased immune responses and the immune regulatory roles of different fibroblast subpopulations in chronic atro... by Lin Lin, Tingxuan Huang, Lizhi Li, Yang Lin, Feng Chen, Ziyi Zheng, Jie Zhou, Yizhe Wang, Weihao You, Yujie Duan, Yawen An, Shiwei He, Weimin Ye

“…The mucous neck cells in CAG lesions showed upregulation of genes related to cell cycle transition, which may lead to aberrant DNA replication. Additionally, cells with the T exhaustion phenotype infiltrated under CAG condition. …”
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Occurrence of Alternaria Toxins, Ochratoxin A and Aflatoxins B1 in Green Arabic Coffee (Coffea arabica) in Saudi Arabia: Risk Assessment on HepG-2 Cell Line and Male Albino Rats by Ahmed Hashim Almusallam, Ahmed Mahmoud Ismail

“…OTA was frequently detected (10 samples, 55.5%) in concentrations varied from 0.75 to 9.87 μg/g. In addition, Aflatoxin B1 was also detected with low quantity ranged 0.89 to 3.22 μg/g. …”
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Clinical Characteristics and Treatment Options of Peripheral Spondyloarthritis by ZENG Lulu, JI Xiaojian, HU Lidong, HU Jiawen, ZHANG Yinan, ZHANG Jiaxin, LIU Xingkang, YANG Shiwei, HUANG Feng

“…The research compared the distribution of swollen and tender joints between pSpA and axSpA patients, as well as that between pSpA1(excluding patients with psoriatic arthritis) and axSpA patients. Additionally, we analyzed differences in clinical features and treatment options among these groups.ResultsA total of 1639 patients were included in the study, of which 184 had pSpA(including 97 with psoriatic arthritis), and 1455 had axSpA. …”
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An integrated microbiome- and metabolome-genome-wide association study reveals the role of heritable ruminal microbial carbohydrate metabolism in lactation performance in Holstein... by Chenguang Zhang, Huifeng Liu, Xingwei Jiang, Zhihong Zhang, Xinfeng Hou, Yue Wang, Dangdang Wang, Zongjun Li, Yangchun Cao, Shengru Wu, Sharon A. Huws, Junhu Yao

“…Conclusion Our comprehensive study revealed the distinct heritability patterns of rumen microbial composition, function, and metabolism. Additionally, we shed light on the influence of host SNP variants on the rumen microbes with carbohydrate metabolism and their subsequent effects on lactation performance. …”
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A compilation of surface inherent optical properties and phytoplankton pigment concentrations from the Atlantic Meridional Transect by T. M. Jordan, G. Dall'Olmo, G. Tilstone, R. J. W. Brewin, F. Nencioli, R. Airs, C. S. Thomas, L. Schlüter

“…</a>, <a href="#bib1.bibx37">2025</a><a href="#bib1.bibx37">a</a>)</span> and <a href="https://doi.org/10.5281/zenodo.12527954">https://doi.org/10.5281/zenodo.12527954</a> <span class="cit" id="xref_paren.2">(<a href="#bib1.bibx35">Jordan et al.…”
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Clinical Observation of Auricular Acupressure Combined with Transcranial Direct Current Stimulation for Nicotine Dependence by KUANG Hongjun, FU Shanshan, HUANG Qian, YANG Layuan, YUAN Yangyang, YUAN Nan, LIU Minquan, TANG Jian, FANG Chuang, ZHONG Feng

“…When the desire to smoke was strong, patients were adviced to press and pinch it for 3 minutes. In addition, the auricular acupressure pads were changed every 2 days, three times per week, for four weeks. …”
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The Effect of Wheat Residues and Vermicompost on the Quality Characteristics of Corn (Zea mays L.) Forage and Soil Fertility by Hasanali Pooresmaeil, Mehdi Dahmradeh, Ahmad Ghanbari

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Protein kinase inhibitors as targeted therapy for glioblastoma: a meta-analysis of randomized controlled clinical trials by José Pinto-Fraga, Celia García-Chico, Simone Lista, Pedro Miguel Lacal, Giuseppe Carpenzano, Maurizio Salvati, Alejandro Santos-Lozano, Grazia Graziani, Claudia Ceci

“…In newly diagnosed GBM, no significant differences were observed in median [-1.02 months, 95 % confidence interval (CI), −2.37–0.32, p = 0.14] and pooled [hazard ratio (HR) = 1.13, 95 % CI, 0.95–1.35, p = 0.17) OS, or in median (0.34 months, 95 % CI, −0.9–1.58, p = 0.60) and pooled (HR = 0.98, 95 % CI, 0.76–1.27, p = 0.89) PFS, when comparing PKI addition to standard chemo-radiotherapy versus chemo-radiotherapy alone. …”
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Effects of Digital Storytelling Package on Students’ Motivation and Attitude to Christian Religious Studies (CRS) in Junior Secondary Schools by Adetunmbi L. Akinyemi, Jonathan Iyanuoluwa Makinde

“…The findings of this study revealed that students exhibit a low level of motivation and a negative attitude towards learning C.R.S. Additionally, there was a significant effect observed from the digital storytelling package on both the motivation and attitude of students (F (2; 61) = 29.67; p = 0.49 for motivation, and F (2; 61) = 32.49; p = 0.52 for attitude). …”
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KiKli Fit: Investigating the Implementation of a Physical Activity Program in Pediatric Cancer by Lars Rehbein, Ann Christin Schneider, Christina Hillebrech, Christina Schindera, Amika Singh, Katharina Eva, Valentin Benzing

“…To address this gap, the KiKli Fit program was developed and implemented at the Department of Pediatric Oncology and Hematology at the University Hospital Bern over the last 1.5 years. …”
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Studies on the High-Power Piezoelectric Property Measurement Methods and Decoupling the Power and Temperature Effects on PZT-5H by Wenchao Xue, Xiaobo Wang, Yuliang Zhu, Chengtao Luo

“…The results from both methods indicated that with increasing power, the electromechanical coupling coefficient <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msub><mrow><mi>k</mi></mrow><mrow><mn>31</mn></mrow></msub></mrow></semantics></math></inline-formula> , the piezoelectric constant <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msub><mrow><mi>d</mi></mrow><mrow><mn>31</mn></mrow></msub></mrow></semantics></math></inline-formula>, and the elastic compliance <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msubsup><mrow><mi>s</mi></mrow><mrow><mn>11</mn></mrow><mrow><mi mathvariant="normal">E</mi></mrow></msubsup></mrow></semantics></math></inline-formula> of the PZT-5H showed increasing trends, while the mechanical quality factor <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msub><mrow><mi>Q</mi></mrow><mrow><mi mathvariant="normal">m</mi></mrow></msub></mrow></semantics></math></inline-formula> first decayed rapidly and then stabilized at a fixed level. Additionally, under the combined impedance method, the temperature of the vibrators rose significantly due to self-heating, whereas the transient method generated almost no heat, and the vibrators remained at room temperature. …”
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Effect of nonsteroidal anti-inflammatory drugs on neonatal calf diarrhea when administered at a disease alert generated by automated milk feeders by A. Welk, M.C. Cantor, H.W. Neave, J.H.C. Costa, J.L. Morrison, C.B. Winder, D.L. Renaud

“…However, calves provided an NSAID had reduced odds of being treated with electrolytes (odds ratio = 0.32, 95% CI: 0.10–0.98). …”
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