Detection of cervical precancerous lesions and cancer by small-scale RT-qPCR analysis of oppositely deregulated mRNAs pairs in cytological smears by Anastasia A. Artyukh, Mikhail K. Ivanov, Mikhail K. Ivanov, Sergei E. Titov, Sergei E. Titov, Victoria V. Dzyubenko, Sergey E. Krasilnikov, Sergey E. Krasilnikov, Anastasia O. Shumeikina, Anastasia O. Shumeikina, Anastasia O. Shumeikina, Nikita A. Afanasev, Anastasia V. Malek, Sergei A. Glushkov, Eduard F. Agletdinov

“…Each classifier outputs a dimensionless decision function (DF) value that increases with lesion severity. Additionally, in the same specimens, the HPV genotyping, viral load assessment, diagnosis of cervicovaginal microbiome imbalance and profiling of some relevant mRNAs and miRNAs were performed by qPCR-based methods.ResultsThe best classifiers were obtained with pairs of mRNAs whose expression changes in opposite directions during lesion progression. …”
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Olanzapine for young PEople with aNorexia nervosa (OPEN): results of a feasibility study by Olena Said, Dominic Stringer, Ece Sengun Filiz, Hiba Mutwalli, Sevgi Bektas, Melahat Nur Akkese, Vanessa Kellermann, Katie Ireland, Elizabeth Tyrrell-Bunge, Demelza Beishon-Murley, Joel W. T. Khor, Lee Allman, Joanna Barker, Nicus Kotze, Ben Carter, Mima Simic, Dilveer Singh Sually, Jessica Bentley, Allan H. Young, Sloane Madden, Sarah Byford, Sabine Landau, Vanessa Lawrence, Janet Treasure, Ulrike Schmidt, Dasha Nicholls, Hubertus Himmerich

“…We estimated the following planning parameters: Recruitment rate (number of patients who agreed to take olanzapine/number eligible), adherence rate (number adhering to treatment/number recruited) and attrition rate (number completing study assessments/number recruited). In addition, two exploratory effect size parameters were estimated: Mean change in body mass index (BMI) and mean change in ED psychopathology. …”
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Endo 180 participates in collagen remodeling of the periodontal ligament during orthodontic tooth movement by Liyuan Chen, Danqing He, Zixin Li, Shengjie Cui, Min Yu, Zimo Zhao, Yuetong Chen, Jiayi Song, Nan Jiang, Huajie Yu, Yan Liu

“…Protein expressions of Endo180, collagen I (COL I) and collagen III (COL III) were analyzed via immunofluorescence staining. Additionally, the mRNA expressions of Endo180, COL I, and COL III in force-induced PDL cells were examined by RT-qPCR in vitro. …”
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The impact of war on people with type 2 diabetes in Ukraine: a survey studyResearch in context by Oksana Sulaieva, Viktoriia Yerokhovych, Sergii Zemskov, Iuliia Komisarenko, Vitalii Gurianov, Volodymyr Pankiv, Oleksandr Tovkai, Tetyana Yuzvenko, Violetta Yuzvenko, Andrii Tovkai, Zlatoslava Shaienko, Tetyana Falalyeyeva, Nadiya Skrypnyk, Taras Romaniv, Nadiya Pasyechko, Taras Krytskyy, Solomiia Danyliuk, Andrii Klantsa, Dmytro Krasnienkov, Oleksandr Gurbych, Nazarii Kobyliak

“…Experience with military actions (p = 0.002), occupation (p = 0.001), internal displacement (p = 0.018) and family member injury or death (p = 0.031) increased HbA1c. In addition, lack of regular glucose monitoring (p < 0.001), consultation by endocrinologists (p < 0.001), diet inconsistency (p = 0.017) and scarcity of physical activity (p = 0.047) affected the HbA1c parameters. …”
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Diagnostics and treatment of Helicobacter pylori infection in adults: Clinical guidelines of the Russian gastroenterological association by V. T. Ivashkin, I. V. Mayev, T. L. Lapina, A. A. Sheptulin, A. S. Trukhmanov, Ye. K. Baranskaya, R. A. Abdulkhakov, O. P. Alekseyeva, S. A. Alekseyenko, N. N. Dekhnich, R. S. Kozlov, I. L. Klyaritskaya, N. V. Korochanskaya, S. A. Kurilovich, M. F. Osipenko, V. I. Simanenkov, A. V. Tkachev, I. B. Khlynov, V. V. Tsukanov

“…Eradication efficacy may be increased by prolongation of treatment up to 14 days, applicaiton of modern PPIs or increase of PPI doze, addition of bismuth tripotassium dicitrate or probiotic. …”
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Beta-variant recombinant SARS CoV-2 vaccine induces durable cross-reactive antibodies against Omicron BA variants by Odile Launay, Rachna Gupta, Tifany Machabert, Eleine Konate, Alexandra Rousseau, Claire Vigne, Francois Beckers, Louis Devlin, Elisabeth Botelho-Nevers, Marine Cachanado, Christian Chidiac, Dominique Deplanque, Bertrand Dussol, Inès Ben Ghezala, Marie Lachatre, Karine Lacombe, Fabrice Laine, Liem Binh Luong Nguyen, Patricia Pavese, Catherine Schmidt-Mutter, Marie-Pierre Tavolacci, Roman M. Chicz, Bogdana Coudsy, Saranya Sridhar, Amel Touati, Eric Tartour, Tabassome Simon

“…A subset of these participants with available blood samples collected at Day 0 (D0), at 28 days (D28), and 3 months (M3) post-booster were contacted for additional testing (195/208 participants). The persistence of cross-neutralizing antibodies, including against Omicron BA.1 and BA.4/5, up to 3 months after boosting was evaluated using a validated pseudovirus neutralization assay. …”
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Construction and Pathogenicity Analysis of a GFPuv-labeled Peanut Ralstonia solanacearum Strain by Jinling HUANG, Yushuang WU, Huiquan TANG, Minyi HUANG, Ruixue YANG, Qiang WANG, Yong YANG, Xiaodan TAN

“…Pathogenicity tests indicated that, the plant wilting mortality rate reached 100% for both PeaFJ1-GFPuv and wild-type PeaFJ1 strains after 11 days of inoculation, with no significant difference. Additionally, measurements of leaf conductivity and bacterial content demonstrated that the proliferation rate of the GFPuv-labeled strain in peanut leaves was comparable to that of the wild-type strain. …”
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Study on the correlation between microstructural evolution and hydrogen storage properties in hyper-eutectic Mg-xNi (x = 15, 20, 25) alloys by So Jin Jung, Seok Su Sohn, Da Hye Lee, Won-Seok Ko, Tae Jun Ha, Ryun-Ho Kwak, Byeong-Chan Suh, Young Min Kim, Hyung-Ki Park

“…To understand why the plateau pressure remained constant despite variations in alloy composition, the equilibrium compositions of the Mg and Mg2Ni phases in the alloys were calculated through thermodynamic calculations. In addition, the compositions of the Mg and Mg2Ni phases within the lamellar structure were analyzed using transmission electron microscope. …”
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Evaluating quality of life improvements in endometriosis patients following laparoscopic surgery using EHP-30 scale by Yuyan Guo, Penghui Huang, Chaobin Liu, Zhenna Wang, Yi Wang, Wei Qi, Xi Xie, Zhenhong Wang, Jinna Zhang, Shunhe Lin

“…Spearman correlation analysis revealed positive correlations between preoperative serum CA125 levels (P = 0.005) and intraoperative rASRM scores with preoperative pain (P = 0.035) and sexual intercourse scores (P = 0.046). Additionally, multivariate linear regression analyses demonstrated that changes in pain scores (ΔPain), control and powerlessness (ΔControl and Powerlessness), and work life (ΔWork Life) were significantly interrelated (P < 0.01). …”
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Systematic bias between blinded independent central review and local assessment: literature review and analyses of 76 phase III randomised controlled trials in 45 688 patients with... by Jianrong Zhang, Yu Huang, Hengrui Liang, Wenhua Liang, Jianxing He, Yiyin Zhang, Jiaxi He, Jieyu Wu, Long Jiang, Shiyan Tang, Qihua He, Lindsey Tristine Hamblin, Zhiheng Xu, Yaoqi Chen, Difei Chen, Xinyu Wang, Kexin Deng, Shuhan Jiang, Jiaqing Zhou, Jiaxuan Xu, Xuanzuo Chen

“…The aim of this study is to investigate whether systematic bias existed in RCTs by comparing the treatment effects of efficacy endpoints between central and local assessments.Design Literature review, pooling analysis and correlation analysis.Data sources PubMed, from 1 January 2010 to 30 June 2017.Eligibility criteria for selecting studies Eligible articles are phase III RCTs comparing anticancer agents for advanced solid tumours. Additionally, the articles should report objective response rate (ORR), disease control rate (DCR), progression-free survival (PFS) or time to progression (TTP); the treatment effect of these endpoints, OR or HR, should be based on central and local assessments.Results Of 76 included trials involving 45 688 patients, 17 (22%) trials reported their endpoints with statistically inconsistent inferences (p value lower/higher than the probability of type I error) between central and local assessments; among them, 9 (53%) trials had statistically significant inference based on central assessment. …”
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Low light reduces saffron corm yield by inhibiting starch synthesis by Weijing Yang, Weijing Yang, Xin Li, Xin Li, Fei Chang, Fei Chang, Xue Qiu, Xulong Huang, Xulong Huang, Zhan Feng, Zhan Feng, Jie Yan, Jie Yan, Qinghua Wu, Qinghua Wu, Feiyan Wen, Feiyan Wen, Jin Pei, Jin Pei, Tao Zhou, Tao Zhou

“…Saffron plants under LL had larger, longer leaves with a higher proportion of dry weight (DW) compared to those under NL. Despite the marked inhibition of photosynthetic capacity, initial DW, sucrose and glucose concentrations in leaves were comparable to those under NL. …”
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Genotypic difference in response to copper stress in upland cotton as revealed by physiological and molecular expression analyses by Jianfei Wu, Tao Wang, Yin Huang, Shuiping Xiao, Xiaoxia Luo, Yanfeng Deng, Xiu Yang, Qingquan Kong, Feiyu Tang

“…However, the total Cu uptake amount per plant did not differ between both lines due to larger plant biomass with A2304. …”
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Chemotherapy-induced cellular senescence promotes stemness of aggressive B-cell non-Hodgkin’s lymphoma via CCR7/ARHGAP18/IKBα signaling activation by Yangyang Wang, Fan Wu, Yang Wan, Huiping Wang, Linhui Hu, Qianshan Tao, Zhimin Zhai, Jiyu Wang, Keke Huang, Anwen Ren, Jinlan Li, Liuying Yi, Yanjie Ruan, Zhixiang Wanyan, Chaohong Liu

“…Finally, inhibitors GS143 and Y27632 were used to examine the effect of IKBα and ARHGAP/RhoA inhibition on B-NHL-TIS.Results Here we identified a distinct group of TIS, composed of memory B-cell population characterized by strong positive expression of CCR7, which was significantly elevated in TIS population compared with normal proliferating and autonomously senescent lymphoma cell populations. Additionally, CCR7 expression was significantly upregulated in patients with r/r B-NHL, and was an independent prognostic factor in B-NHL, with high CCR7 expression being strongly associated with poor prognosis. …”
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Is the freezing index a valid outcome to assess freezing of gait during turning in Parkinson’s disease? by Maaike Goris, Pieter Ginis, Clint Hansen, Christian Schlenstedt, Christian Schlenstedt, Jeffrey M. Hausdorff, Jeffrey M. Hausdorff, Jeffrey M. Hausdorff, Nicholas D’Cruz, Wim Vandenberghe, Wim Vandenberghe, Walter Maetzler, Alice Nieuwboer, Moran Gilat

“…Therefore, this study aimed to evaluate the FOG-index’s classification performance and criterion validity to assess FOG severity during 360 turn. Additionally, we investigated the FOG-index’s optimal cutoff values to differentiate between PwPD with and without FOG.Methods164 PwPD self-reported the presence of FOG on the New Freezing of Gait Questionnaire (NFOGQ) and performed the DT 360 turn in the ON medication state while being videoed and wearing five wearable sensors. …”
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Intestinal butyric acid-mediated disruption of gut hormone secretion and lipid metabolism in vasopressin receptor-deficient mice by Kazuki Harada, Eiji Wada, Yuri Osuga, Kie Shimizu, Reiko Uenoyama, Masami Yokota Hirai, Fumihiko Maekawa, Masao Miyazaki, Yukiko K. Hayashi, Kazuaki Nakamura, Takashi Tsuboi

“…Exposure to butyric acid reduced GLP-1 secretion in L cell line. Additionally, human Paneth cell-related gene expressions negatively correlated with that of V1 receptor genes. …”
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The First Large Identification of 3ANX and NX Producing Isolates of <i>Fusarium graminearum</i> in Manitoba, Western Canada by Maria Antonia Henriquez, Srinivas Sura, Sean Walkowiak, David Kaminski, Anne Kirk, Mark W. Sumarah, Parthasarathy Santhanam, Nina Kepeshchuk, Jules Carlson, E. RoTimi Ojo, Pam de Rocquigny, Holly Derksen

“…This study highlights the first large identification of 3ANX- and NX-producing isolates of <i>F. graminearum</i> in Western Canada. In addition, it is the first identification of 15ADON chemotypes producing 3ANX in Western Canada and the first identification of 3ANX and NX-producing isolates in Manitoba, collected from wheat samples.…”
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PINK1 modulates Prdx2 to reduce lipotoxicity‐induced apoptosis and attenuate cardiac dysfunction in heart failure mice with a preserved ejection fraction by Hao Zhang, Tianyu Xu, Xiyuan Mei, Qiming Zhao, Qiling Yang, Xianghui Zeng, Zhuang Ma, Haobin Zhou, Qingchun Zeng, Dingli Xu, Hao Ren

“…Key points Our investigation discloses a pivotal relationship between PINK1 and Prdx2 in the context of HFpEF. Notably, PINK1, in addition to its role in mitochondrial autophagy, can increase Prdx2 expression, effectively remove ROS and attenuate cardiomyocyte apoptosis by modulating the JNK and p38 pathways, thereby alleviating myocardial lipotoxicity and improving HFpEF cardiac function. …”
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Metabolomic profiling and antibacterial efficacy of probiotic-derived cell-free supernatant encapsulated in nanostructured lipid carriers against canine multidrug-resistant bacteri... by Nay Zin Myo, Nay Zin Myo, Ratchnida Kamwa, Ratchnida Kamwa, Thitirat Jamnong, Busaba Swasdipisal, Papavarin Somrak, Phanchompoo Rattanamalakorn, Vipada Neatsawang, Prasert Apiwatsiri, Teerapong Yata, David J. Hampson, Nuvee Prapasarakul, Nuvee Prapasarakul

“…AimThis study aimed to investigate the antibacterial efficacy of probiotic-derived cell-free supernatants (CFS) encapsulated within nanostructured lipid carriers (NLCs) against multidrug-resistant Pseudomonas aeruginosa and Staphylococcus pseudintermedius. Additionally, it aimed to identify specific bioactive compounds that contribute to the reported antibacterial properties by characterizing the metabolite substances present in the CFS using a metabolomic analysis technique.MethodsEight strains of lactic acid bacteria including Lactiplantibacillus plantarum (L22F and L25F), Pediococcus acidilactici (P72N, BF9, BF 14, BYF 20 and BYF 26) and Ligilactobacillus salivarius (BF 12) were selected as probiotic candidates. …”
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The effect of renal function on the clinical outcomes and management of patients hospitalized with hyperglycemic crises by Sumaya N. Almohareb, Sumaya N. Almohareb, Sumaya N. Almohareb, Norah Aljammaz, Nada Yousif, Mayar Sunbul, Raghad Alsemary, Lama Alkhathran, Mohammed Aldhaeefi, Omar A. Almohammed, Abdulrahman I. Alshaya, Abdulrahman I. Alshaya, Abdulrahman I. Alshaya

“…Larger, multi-center studies are needed to validate these findings and explore additional factors that may impact the management of DKA in patients with CKD.…”
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Exploring the role of ESR1 mutations in metastatic hormone receptor-positive breast cancer T cell immune surveillance disruption by Morgane Lopez, Laurie Spehner, Fabrice André, Julien Viot, Evan Seffar, Amélie Marguier, Elsa Curtit, Guillaume Meynard, Erion Dobi, Sylvain Ladoire, Romain Boidot, Romain Loyon, Valentin Derangere, François-Clément Bidard, Christophe Borg, Laura Mansi, Marie Kroemer

“…Our results showed that 40% of patients had specific immune responses. In addition, we demonstrated the HLA-A2 ESR1 peptide immunogenicity in humanized HLA-A2/DR1 mice. …”
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