Preclinical pharmaco-toxicological screening of biomimetic melanin-like nanoparticles as a potential therapeutic strategy for cutaneous melanoma by Iasmina Marcovici, Iasmina Marcovici, Raul Chioibas, Raul Chioibas, Istvan Zupko, Iulia Pinzaru, Iulia Pinzaru, Alina Moaca, Alina Moaca, Adriana Ledeti, Adriana Ledeti, Lucian Barbu-Tudoran, Lucian Barbu-Tudoran, Andreea Geamantan, Andreea Geamantan, Iasmina Predescu, Iasmina Predescu, Cristina Adriana Dehelean, Cristina Adriana Dehelean

“…After a 24 h treatment, MEL-NPs exerted a selective cytotoxicity in SH-4 and B164A5 CM cells compared to HEMa, HaCaT, and JB6 Cl 41-5a healthy skin cells, except for the concentration of 100 µg/mL, at which their viability declined under 70%. Additionally, MEL-NPs accumulated within the intracellular space of CM cells, forming a perinuclear coating, inhibited their motility and clonogenic potential, increased intracellular oxidative stress, targeted the epithelial-to-mesenchymal transition, and induced apoptosis by altering cell morphology, nuclear aspect, F-actin and tubulin distribution, and by modulating the expression of pro- and anti-apoptotic markers. …”
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Climate change rivals fertilizer use in driving soil nitrous oxide emissions in the northern high latitudes: Insights from terrestrial biosphere models by Naiqing Pan, Hanqin Tian, Hao Shi, Shufen Pan, Josep G. Canadell, Jinfeng Chang, Philippe Ciais, Eric A. Davidson, Gustaf Hugelius, Akihiko Ito, Robert B. Jackson, Fortunat Joos, Sebastian Lienert, Dylan B. Millet, Stefan Olin, Prabir K. Patra, Rona L. Thompson, Nicolas Vuichard, Kelley C. Wells, Chris Wilson, Yongfa You, Sönke Zaehle

“…The trend of increasing emissions from nitrogen fertilizer reversed after the 1980 s because of reduced applications in non-permafrost regions. In addition, increased plant growth due to CO2 fertilization suppressed simulated emissions. …”
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Association of blood group types and clinico-pathological features of gynecological cancers (GCs) by Syed Sameer Aga, Muhammad Anwar Khan, Mubarak Al Mansour, Rana Mohammed Hasosah, Lulu Abdullah Alsubaie, Dala Ahmad Alfaify, Malak Badr Alansari, Shahad Obaidallah Almutairi, Saniya Nissar

“…Furthermore, there was a significant association between BG B and endometrial and serous histology in fallopian tube and between BG AB and ovarian malignancies, respectively. Additionally, multinomial analysis revealed higher risk of Cervical, Endometrial and Ovarian cancers for Type B BG (P < 0.05).…”
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Development of a Cationic Polymeric Micellar Structure with Endosomal Escape Capability Enables Enhanced Intramuscular Transfection of mRNA-LNPs by Siyuan Deng, Han Shao, Hongtao Shang, Lingjin Pang, Xiaomeng Chen, Jingyi Cao, Yi Wang, Zhao Zhao

“…<b>Methods:</b> We synthesized a cationic and ampholytic di-block copolymer, poly (poly (ethylene glycol)_4-5 methacrylate_a-<i>co</i>-hexyl methacrylate_b)_X-<i>b</i>-poly(butyl methacrylate_c-<i>co</i>-dimethylaminoethyl methacrylate_d-<i>co</i>-propyl acrylate_e)_Y (p(PEG_4-5MA_a-<i>co</i>-HMA_b)_X-<i>b</i>-p(BMA_c-<i>co</i>-DMAEMA_d-<i>co</i>-PAA_e)_Y), via reversible addition–fragmentation chain transfer polymerization. …”
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Effects of group communication norms on daily steps in a team-based financial incentive mobile phone intervention in Shanghai, China by Yingnan Jia, Yingcheng Xiao, Hao Chen, Klaus Gebel, Chengshu Li, Shuangyuan Sun, Qinping Yang, Siyuan Wang, Li Zhang, Jing Wang, Minna Cheng, Dantong Gu, Yan Shi, Ding Ding

“…The teams and individual members would receive points for meeting the daily goal, contributing to team-based rankings and financial rewards for the teams and their members. In addition, the intervention teams created dedicated WeChat groups to facilitate communication, which were also used to collect group chat messages. …”
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Deep learning helps discriminate between autoimmune hepatitis and primary biliary cholangitis by Alessio Gerussi, Oliver Lester Saldanha, Giorgio Cazzaniga, Damiano Verda, Zunamys I. Carrero, Bastian Engel, Richard Taubert, Francesca Bolis, Laura Cristoferi, Federica Malinverno, Francesca Colapietro, Reha Akpinar, Luca Di Tommaso, Luigi Terracciano, Ana Lleo, Mauro Viganó, Cristina Rigamonti, Daniela Cabibi, Vincenza Calvaruso, Fabio Gibilisco, Nicoló Caldonazzi, Alessandro Valentino, Stefano Ceola, Valentina Canini, Eugenia Nofit, Marco Muselli, Julien Calderaro, Dina Tiniakos, Vincenzo L’Imperio, Fabio Pagni, Nicola Zucchini, Pietro Invernizzi, Marco Carbone, Jakob Nikolas Kather

“…The scientific justification for this work lies in addressing the challenge of differentiating these conditions, which often present with overlapping features and can lead to therapeutic mistakes. In addition, there is need for quantitative assessment of information embedded in liver biopsies, which are currently evaluated on qualitative or semi-quantitative methods. …”
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Persistent organochlorine pesticides and cardiometabolic outcomes among middle-aged Latina women in a California agricultural community: The CHAMACOS Maternal Cognition Study by Marcella Warner, Stephen Rauch, Brenda Eskenazi, Lucia Calderon, Robert B. Gunier, Katherine Kogut, Nina Holland, Weihong Guo, Julianna Deardorff, Jacqueline M. Torres

“…Although credible intervals crossed the null, consistent positive associations were observed for DDT and β-HCH with blood pressure and for DDT with insulin resistance. …”
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AICpred: Machine Learning-Based Prediction of Potential Anti-Inflammatory Compounds Targeting TLR4-MyD88 Binding Mechanism by Lucindah N. Fry-Nartey, Cyril Akafia, Ursula S. Nkonu, Spencer B. Baiden, Ignatus Nunana Dorvi, Kwasi Agyenkwa-Mawuli, Odame Agyapong, Claude Fiifi Hayford, Michael D. Wilson, Whelton A. Miller, Samuel K. Kwofie

“…Toll-like receptor 4 (TLR4) has been implicated in the production of uncontrolled inflammation within the body, known as the cytokine storm. Studies that employ machine learning (ML) in the prediction of potential inhibitors of TLR4 are limited. …”
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HPLC-MS standardization and validation methods for determination of calactin content in dichloromethane fraction of Calotropis gigantea (L.) Dryand. (Niu jiao gua) stem bark and it... by Naphat Kaewpaeng, Sudaporn Wongwan, Khemmachat Pansooksan, Chanakan Chailom, Pattaraporn Chaisupasakul, Dumrongsak Pekthong, Nitra Nuengchamnong, Zhi-Hong Jiang, Li-Ping Bai, Piyarat Srisawang, Supawadee Parhira

“…Therefore, the method was suitable for the quality control of CGD. Additionally, we suggest applying the established protocol to quantify the calactin contents in CGDs collected over a period of one year (12 months) to investigate the correlation to their cytotoxicities against human cell lines derived from colon cancer (HCT116), human hepatoblastoma (HepG2) and human cell line derived from stomach cancer metastasized to liver (MKN74). …”
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Sex and outcomes of patients with microsatellite instability-high and BRAF V600E mutated metastatic colorectal cancer receiving immune checkpoint inhibitors by Michael Hoffmeister, Hermann Brenner, Chiara Cremolini, Sara Lonardi, Filippo Pietrantonio, Julien Taieb, David Tougeron, Robyn L Ward, Marco Vitellaro, Thierry André, Pierre Laurent-Puig, Jitendra Jonnagaddala, Francesca Bergamo, Raghav Sundar, Claire Gallois, Michael J Overman, Jakob Nikolas Kather, Lisa Salvatore, Romain Cohen, Rossana Intini, Priya Jayachandran, Miriam Koopman, Javier Ros, Marwan Fakih, Vincenzo Nasca, Giacomo Mazzoli, Jeanine M L Roodhart, Filippo Ghelardi, Elena Elez, Durgesh Wankhede, Marta Ligero, Joseph Zhao, Koen Zwart, Jeroen Derksen, Nicholas Hawkins, Javier Carmona

“…Males receiving anti-PD-(L)1 monotherapy had the worst outcomes, with a 3-year PFS and 3-year OS of 23.9% and 41.8%, respectively, while the addition of anti-CTLA-4 agents rescued such a worse outcome. …”
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Virtual reality-based cognitive–behavioural therapy for the treatment of anxiety in patients with acute myocardial infarction: a randomised clinical trial by Jiajun Weng, Junbo Ge, Jiajia Liu, Juying Qian, Xiao Huang, Yuanyuan Li, Juan Peng, Yan'e Lu, Dong Huang, Pingyuan Yang, Shikun Xu, Lihua Guan, Qibing Wang, Zexin Zhao, Yabin Wei

“…Patients who were Han Chinese aged 18–75 years were randomly assigned (1:1) via block randomisation to either the VR-CBT group to receive VR-CBT in addition to standard mental health support, or the control group to receive standard mental health support only. …”
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Expression of the NUP153 and YWHAB genes from their canonical promoters and alternative promoters of the LINE-1 retrotransposon in the placenta of the first trimester of pregnancy by V. V. Demeneva, E. N. Tolmacheva, T. V. Nikitina, E. A. Sazhenova, S. Yu. Yuriev, A. Sh. Makhmutkhodzhaev, A. S. Zuev, S. A. Filatova, A. E. Dmitriev, Ya. A. Darkova, L. P. Nazarenko, I. N. Lebedev, S. A. Vasilyev

“…Due to this feature of the placenta, there is a potential possibility of using regulatory elements derived from retroviruses and retrotransposons, which are suppressed by DNA methylation in the adult body. In addition, there is an abnormal increase in the level of methylation of the LINE-1 retrotransposon in the chorionic trophoblast in spontaneous abortions with both normal karyotype and aneuploidy on different chromosomes, which may be associated with impaired gene transcription using LINE-1 regulatory elements. …”
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The Effect of Vascular Endothelial Growth Factor on Bone Marrow Mesenchymal Stem Cell Engraftment in Rat Fibrotic Liver upon Transplantation by Ke Yuan, Chunyou Lai, Lingling Wei, Tianhang Feng, Qinyan Yang, Tianying Zhang, Tao Lan, Yutong Yao, Guangming Xiang, Xiaolun Huang

“…We confirmed the expression of very late antigen-4 (VLA4, integrin α4β1) on rat BMSCs and the elevated expression of vascular adhesion molecule-1 (VCAM-1) in the hepatic sinusoidal endothelial cells. In addition, the analysis of CM-DiI-labeled BMSCs showed that the BMSC+VEGF group exhibited better cell engraftment and that the engrafted cells were mainly distributed in the hepatic parenchyma. …”
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Case Report: Efficacy and safety of recombinant growth hormone therapy in a girl with Loeys–Dietz syndrome by Kamil Dyrka, Aleksander Jamsheer, Michal Bartecki, Waldemar Bobkowski, Malgorzata Pawelec-Wojtalik, Justyna Rajewska-Tabor, Andzelika Tomaszewska, Justyna Balcerzak, Zuzanna Aniol, Marek Niedziela, Monika Obara-Moszynska

“…However, whether IGF-1, the main biochemical marker of GH activity, can be independently associated with increased aortic diameter has not been determined. In addition to its growth-promoting effect, the wide influence of GH on the human body, metabolic status, and muscle strength is also significant. …”
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Impact of gender on self-assessment accuracy among fourth-year French medical students on faculty’s online Objective Structured Clinical Examinations by Sylvain Bodard, Donia Bouzid, Valentine Marie Ferré, Claire Carette, Joelle Kivits, Yann Nguyen, Michael Thy, Ugo Marchèse, Bénédicte Oulès, Lina Khider, Christian de Tymowski, Nelly Burnichon, Tristan Mirault, Albert Faye, Laura I. Levi

“…Students and evaluators assessed performances using 5-point Likert scales for medical knowledge, interpersonal skills, and overall performance. Additionally, students predicted their grade out of twenty. …”
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Obstructive sleep apnea and structural and functional brain alterations: a brain-wide investigation from clinical association to genetic causality by Kang Wu, Qiming Gan, Yuhong Pi, Yanjuan Wu, Wenjin Zou, Xiaofen Su, Sun Zhang, Xinni Wang, Xinchun Li, Nuofu Zhang

“…Methods We studied 20 pairs of age-, sex-, BMI-, and education- matched OSA patients and healthy controls using multimodal magnetic resonance imaging (MRI) from August 2019 to February 2020. Additionally, large-scale Mendelian randomization analyses were performed using genome-wide association study (GWAS) data on OSA and 3935 brain imaging-derived phenotypes (IDPs), assessed in up to 33,224 individuals between December 2023 and March 2024, to explore potential genetic causality between OSA and alterations in whole brain structure and function. …”
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Molecular-level characterization of supraglacial dissolved and water-extractable organic matter along a hydrological flow path in a Greenland Ice Sheet micro-catchment by E. L. Doting, E. L. Doting, I. T. Stevens, A. M. Kellerman, P. E. Rossel, R. Antony, R. Antony, A. M. McKenna, A. M. McKenna, M. Tranter, L. G. Benning, L. G. Benning, R. G. M. Spencer, J. R. Hawkings, J. R. Hawkings, A. M. Anesio

“…Hence, we conclude that the weathering crust plays a thus far unexplored role in supraglacial biogeochemical cycling. In addition, we characterize water-extractable organic matter isolated from surface ice particulate matter, which was predominantly (61.6 <span class="inline-formula">±</span> 8.1 % relative abundance) comprised of aliphatic and peptide-like formulae, providing the first direct evidence of surface ice particulate matter as a potential source of biolabile DOM. …”
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Factors associated with non-response and nutritional status of non-responders at 6-month post-discharge: a cohort study nested in a MUAC-based nutrition programme for acutely malno... by Maguy Daures, Jérémie Hien, Cécile Cazes, Rodrigue Alitanou, Laure Saillet, Benjamin Séri, Ahmad Ag Mohamed Aly, Oumarou Maidadji, Atté Sanoussi, Aboubacar Mahamadou, Mathias Altmann, Kevin Phelan, Renaud Becquet, Susan Shepherd

“…As efforts to expand MUAC-based programming progress, adapting exit criterion and/or providing additional food supplementation with smaller daily ration for children with risk factors discussed here may help improve programme efficiency without adding to the cost of treatment.…”
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Community-based HPV self-collection versus visual inspection with acetic acid in Uganda: a cost-effectiveness analysis of the ASPIRE trial by Joel Singer, Angeli Rawat, Heather Armstrong, Josaphat Byamugisha, Gina S Ogilvie, Alex K Mezei, Heather N Pedersen, Stephen Sy, Catherine Regan, Sheona M Mitchell-Foster, Musa Sekikubo, Jane J Kim, Nicole G Campos

“…In both arms, VIA was performed at the local health unit by midwives with VIA-positive women receiving immediate treatment with cryotherapy.Design We informed a Monte Carlo simulation model of HPV infection and cervical cancer with screening uptake, compliance and retrospective cost data from the ASPIRE trial; additional cost, test performance and treatment effectiveness data were drawn from observational studies. …”
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Shaoyao Gancao decoction alleviates paclitaxel-induced cognitive impairment by activating PTEN/PI3K/AKT pathway to inhibit NETs formation by Xu Ying, Su Yue, Hu Yuwen, Li Xiang, Zhou Ziyan, Yuan Ningning, Ji Xiaowei, Jiang Ruoyu, Wang Wenzhu, Zhang Yafeng, Zhai Guojie, Cheng Xiaolan

“…Furthermore, SGD remarkably reduced IL-1β, TNF-α and microglia activation, while enhanced SOD activity in hippocampus. In addition, SGD could inhibit NETs formation, evidenced by decreasing the level of plasma MPO-DNA complex, as well as MPO and PAD4 expression. …”
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