Theoretical Study of Sphingomyelinases from <i>Entamoeba histolytica</i> and <i>Trichomonas vaginalis</i> Sheds Light on the Evolution of Enzymes Needed for Survival and Colonizati... by Fátima Berenice Ramírez-Montiel, Sairy Yarely Andrade-Guillen, Ana Laura Medina-Nieto, Ángeles Rangel-Serrano, José A. Martínez-Álvarez, Javier de la Mora, Naurú Idalia Vargas-Maya, Claudia Leticia Mendoza-Macías, Felipe Padilla-Vaca, Bernardo Franco

“…Cavity blind docking showed that the substrate is pushed outward of the active site when cobalt is bound instead of magnesium ions. Additionally, the structural models of the aSMase6 of <i>E. histolytica</i> showed a loop that is absent from the rest of the aSMases, suggesting that it may be involved in hemolytic activity, as demonstrated experimentally using the recombinant proteins of aSMase4 and aSMase6. …”
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Targeting the NLRP3 inflammasome abrogates cardiotoxicity of immune checkpoint blockers by Ping Li, Zhen Qin, Yang Lu, Jiamin Gao, Zhenwei Wang, Ge Zhang, Han Yang, Yachen Hou, Dashuai Wang, Pengchong Du, Yunzhe Wang, Quanzhou Chen, Zhaowei Sun, Jinying Zhang, Junnan Tang

“…Single-cell RNA sequencing elucidated that MCC950 treatment reduced the cardiac infiltration of pathogenic macrophages, cytotoxic T cells, activated T cells, and their production of inflammatory cytokines, while enhancing the presence of reparative macrophages and naive T cells. In addition, MCC950 attenuated cardiotoxicity induced by dual programmed cell death protein 1 (PD-1) and cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) immunotherapy and promoted tumor regression, and showed efficacy in treating established cardiotoxicity.Conclusions Our findings provide a promising clinical approach for preventing and treating cardiotoxicity induced by ICIs, dissociating the antitumor efficacy of ICI-based therapies from their cardiotoxic side effects.…”
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Comparison of Aerodynamic Effects on the Commonwealth Advisory Aeronautical Research Council (CAARC) Tall Building Model Tested in Two Wind Tunnel Laboratories by Yuhao Zhu, Xu Zhou, Yong Chen, Chenyan Ma, Lingjun Wang, Chaorong Zheng, Bowen Yan

“…This is due to the static pressure which significantly decreases the streamwise direction within the test section, and the difference in static pressure is logarithmically proportional to the mean wind speed. Additionally, dynamic pressure is uniformly distributed along the test section axis. …”
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Effect of Carbonation on the Leachability of Solidified/Stabilized Lead-Contaminated Expansive Soil by Fusheng Zha, Congmin Liu, Bo Kang, Long Xu, Chengbin Yang, Chengfu Chu, Chuang Yu, Wei Zhang, Jiwen Zhang, Zhenghong Liu

“…The results indicated that the amount of Pb2+ and Ca2+ leached out under different pH conditions increased after carbonation, which confirmed that carbonate on solidified/stabilized lead (Pb) had a negative impact. Additionally, the De values of Pb2+ and Ca2+ varied in the range of 1.16E − 10 cm2/s to 1.71E − 07 cm2/s, which demonstrated that ion migration was low. …”
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RfxCas13d-mediated inhibition of Circ1647 alleviates renal fibrosis via PI3K/AKT signaling pathway by Yufang Ni, Hongmin Zhang, Qianwen Xian, Wenjie Qin, Hongwei Su, Li Wang, Jianchun Li

“…Meanwhile, Immunohistochemistry, qRT-PCR and Western blot were conducted to assess the degree of fibrosis. In addition, overexpressing of Circ1647 in renal tubular epithelial cells (TCMK1) was performed to investigate the underlying mechanisms of Circ1647.Results Our results displayed that electroporation-mediated knockdown of Circ1647 by RfxCas13d knockdown plasmid significantly inhibited renal fibrosis in UUO mice as evidenced by reduced expression of fibronectin and α-SMA (alpha-smooth muscle actin). …”
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Identification of blood transcriptome modules associated with suicidal ideation in patients with major depressive disorder by Min Wang, Hailin Xiang, Jinxue Wei, Yikai Dou, Yushun Yan, Yue Du, Huanhuan Fan, Liansheng Zhao, Rongjun Ni, Xiao Yang, Xiaohong Ma

“…Results indicated that the magenta module (associated with RNA splicing processes) differentiated MDD_SI from MDD_nSI (p = 0.021), while the green module (related to immune and inflammatory responses) distinguished MDD_SI from HC (p = 0.004). …”
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Soft computing approaches of direct torque control for DFIM Motor's by Zakariae Sakhri, El-Houssine Bekkour, Badre Bossoufi, Nicu Bizon, Mishari Metab Almalki, Thamer A.H. Alghamdi, Mohammed Alenezi

“…Results show that DTC-ANN and DTC-SVM stand out for their ripple reduction performance, making them particularly suitable for applications requiring high precision. Additionally, DTC-FL and DTC-SMC excel in robustness against system parameter variations, a valuable asset for evolving industrial environments. …”
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Long-term effect of island industrial construction on groundwater health risk by Yong Huang, Daqing Wang, Haoli Xu, Zhao Lu, Zijian Cheng, Zhibin Ding, Yi Wang, Yue Shi, Huiru Cui, Xiaoying Lian

“…The analysis revealed that the contributions of indicators D and L to the uncertainty were the highest. In addition, the diffusion of chemical oxygen demand in the vicinity of pollution sources has a significant impact on uncertainty. …”
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The Wnt/β-Catenin Pathway Regulated Cytokines for Pathological Neuropathic Pain in Chronic Compression of Dorsal Root Ganglion Model by Ye Zhang, Dan Zhao, Xutong Li, Beiyao Gao, Chengcheng Sun, Shaoting Zhou, Yanhong Ma, Xuemei Chen, Dongsheng Xu

“…Immunofluorescence results also confirmed nuclear translocalization of active β-catenin in DRG and SCDH. Additionally, rats had obvious mechanical induced pain after CCD surgery and the pain was significantly alleviated after the application of the Wnt/β-catenin pathway inhibitor XAV939. …”
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Lipid metabolism indicators provide tools for the diagnosis of non-alcoholic fatty liver disease: results of a nationwide survey by Yongxin Wang, Chang Fu, Hengwei Jin, Yibo Yang, Xiaocong Li, Xiaocong Li, Kai Liu

“…To further elucidate this connection, we utilized restricted cubic splines and conducted subgroup analysis. Additionally, the receiver operating characteristics (ROC) curve was employed to evaluate the predictive effectiveness of CMI, VAI, and LAP for NAFLD.ResultsThe study included 2,878 adults as the study population, of whom 1,263 participants were diagnosed with NAFLD. …”
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Realizing high power factor and thermoelectric performance in band engineered AgSbTe2 by Yu Zhang, Congcong Xing, Dongyang Wang, Aziz Genç, Seng Huat Lee, Cheng Chang, Zhi Li, Luyao Zheng, Khak Ho Lim, Hangtian Zhu, Rabeya Bosry Smriti, Yu Liu, Shaobo Cheng, Min Hong, Xiaolei Fan, Zhiqiang Mao, Li-Dong Zhao, Andreu Cabot, Tiejun Zhu, Bed Poudel

“…Besides, the doped materials exhibit a significantly improved Seebeck coefficient by inhibiting bipolar conductivity and preventing the formation of n-type Ag2Te. Additionally, the optimized AgSbTe2 is used to fabricate a unicouple thermoelectric device that achieves energy conversion efficiencies of up to 12.1% and a high power density of 1.13 Wcm−2. …”
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The Value of Voltage Histogram Analysis Derived Right Atrial Scar Burden in the Prediction of Left Atrial Scar Burden by Szilvia Herczeg, Joseph Galvin, John J. Keaney, Edward Keelan, Roger Byrne, Claire Howard, Laszlo Geller, Gabor Szeplaki

“…We have included 36 patients who underwent catheter ablation for AF in a prospective observational study. In addition to LA mapping and ablation, high-density RA bipolar voltage maps (HD-EAM) were also reconstructed. …”
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GC1126A, a novel ADAMTS13 mutein, evades autoantibodies in immune-mediated thrombotic thrombocytopenic purpura by Heechun Kwak, Gahee Choi, Suyong Kim, Ji-Min Park, Youngeun Kwon, Yongmin Lee, Chaemok Lee, Shangbin Yang, Spero Cataland, Sunghyun Kim, Soo-Mee Bang, Jae-Ho Yoon, Wooin Lee, Hyun-Ja Nam

“…In the iTTP-mimic mouse model, GC1126A treatment significantly improved platelet counts, lactate dehydrogenase levels, and ADAMTS13 residual activity. In addition, GC1126A outperformed recombinant human wild-type ADAMTS13 (rh WT-ADAMTS13) and caplacizumab in terms of platelet recovery and sustained effectiveness. …”
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Associations between surrogate insulin resistance indexes and osteoarthritis: NHANES 2003–2016 by Youmian Lan, Qiongbing Zheng, Meijing Li, Jiexin Chen, Dongyang Huang, Ling Lin

“…Receiver operating characteristic curves indicated that TyG-WHtR (AUC 0.633) demonstrated more robust diagnostic efficacy. Additionally, the sensitivity analysis produced results consistent with the primary findings. …”
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Quantifying human genome parameters in aging by V. P. Volobaev, S. S. Kunizheva, L. I. Uralsky, D. A. Kupriyanova, E. I. Rogaev

“…A separate section of this review is devoted to the correlation of telomere length parameters with age, as well as the association of telomere length with the amount of mitochondrial DNA. In addition, we consider such a quantitative feature as the rate of accumulation of somatic mutations with aging in relation to the lifespan of living organisms. …”
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Billing models for measuring nursing care in inpatient and outpatient settings: a scoping review by Marco Di Nitto, Francesca Napolitano, Michela Calzolari, Yari Longobucco, Vittorio Masotta, Francesco Zaghini, Rosaria Alvaro, Giancarlo Cicolini, Loreto Lancia, Duilio Fiorenzo Manara, Laura Rasero, Gennaro Rocco, Maurizio Zega, Beatrice Mazzoleni, Loredana Sasso, Annamaria Bagnasco

“…We consulted MEDLINE (Pubmed), CINAHL (EBSCOhost), Web of Science (Clarivate), SCOPUS (Elsevier), ProQuest and Google Scholar, government sites and major international bodies (e.g., National Health Service NHS, World Health Organization WHO). …”
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Efficacy comparison of optimal natural orifice specimen extraction for robotic middle rectal cancer resection in women: transanal or transvaginal orifice by Yao Yao, Jiarui Ding, Houqiong Ju, Lingling Yang, Yang Liu, Yahang Liang, Yuli Yuan, Taiyuan Li, Xiong Lei

“…Results Anal function, assessed by the Wexner score, was better in the RATV group than in the RATA group (P = 0.034). Additionally, pre-menopausal women in RATV group exhibited superior anal function over those in RATA group (P = 0.031). …”
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A novel histone acetylation-associated gene signature with prognostic value in Ewing sarcoma by Anshun Wu, Fayin Liu, Lei Zhou, Runyi Jiang, Shangjiang Yu, Zihuan Zhou, Qi Zhang, Qian Zhang, Dongjie Jiang, Shaohui He, Haifeng Wei

“…The 1-, 2-,3- and 5-year AUC (0.853, 0.886,0.909and 0.833, respectively) showed the good ability of this signature to predict the prognoses of EWS patients. In addition, distinct functional enrichment and immune-related pathways were also observed in two risk groups. …”
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Full-length transcriptome analysis of male and female gonads in Japanese Eel (Anguilla japonica) by Jiangling Li, Jingjie Liang, Mengyang Wang, Yuewen Jiang, Wen Li, Mingxi Huang, Yan Huang, Yangjie Xie, Jianchun Chen, Tiansheng Chen

“…A total of 24,661 unigenes were identified in ovaries and 15,023 in testes, along with genomic regulatory elements such as transcription factors, simple sequence repeats, and long non-coding RNAs. Additionally, 1,210 differentially expressed genes were detected. …”
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Visual and High-Efficiency Secretion of SARS-CoV-2 Nanobodies with <i>Escherichia coli</i> by Shuai Zhao, Wanting Zeng, Fang Yu, Pingping Xu, Chin-Yu Chen, Wanping Chen, Yanming Dong, Fei Wang, Lixin Ma

“…The novel nanobody production system enhances the efficiency of nanobody expression and streamlines the downstream purification process, enabling large-scale, cost-effective nanobody production. In addition, <i>E. coli</i> cells secreting the nanobodies on their surface facilitates screening and characterization of antigen-binding clones.…”
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