Isolation of PCSK9-specific nanobodies from synthetic libraries using a combined protein selection strategy by Apisitt Thaiprayoon, Yodpong Chantarasorn, Worrapoj Oonanant, Anongnard Kasorn, Phoomintara Longsompurana, Satita Tapaneeyakorn, Pinpunya Riangrungroj, Fabien Loison, Andrew C. Kruse, Matthew P. DeLisa, Dujduan Waraho-Zhmayev

“…We anticipate that our strategy will be a valuable addition to the protein engineering toolkit, allowing development of Nbs or virtually any other synthetic binding protein for a wide range of applications.…”
Get full text

CENPN contributes to pancreatic carcinoma progression through the MDM2-mediated p53 signaling pathway by Ming Xu, Jie Tang, Qiong Sun, Jing Meng, Guoyu Chen, Yunli Chang, Yao Yao, Jieru Ji, Hao Luo, Lingling Chen, Minxue Lu, Weiwei Shi

“…Reduced CENPN expression impeded PAAD cell proliferation, migration, and invasion; however, these effects were counteracted upon upregulation of CENPN expression. Additionally, CENPN interacted with MDM2, promoting PAAD progression by targeting the p53 signaling pathway. …”
Get full text

Water content determination in different varieties of Cannabis flowers by Martina Miloshevska1, Irena Slaveska Spirevska, Natalija Nakov, Gjose Stefkov, Aneta Dimitrovska, Jelena Acevska

“…The small sample amount, in addition to the simple operation conditions and fast analysis allows the use of the method for ‘in process’ control for Cannabis flowers. …”
Get full text

Exploratory analysis of a Novel RACK1 mutation and its potential role in epileptic seizures via Microglia activation by Sai Zhang, Zhaofei Dong, Jing Guo, Ze Li, Hong Wu, Linming Zhang, Fuli Min, Tao Zeng

“…In vivo experiments demonstrated that RACK1-p.L206P mutant mice displayed classic seizure symptoms, with increased neuronal excitability and a tendency for action potential bursts during initial depolarization, along with more frequent spike discharges. Additionally, excitatory synapse density and size in the hippocampal CA1 region of mutant mice were significantly elevated, accompanied by increased expression of VGLUT1 and PSD95 within microglia. …”
Get full text

Comprehensive Bibliographic Survey and Forward-Looking Recommendations for Software Defect Prediction: Datasets, Validation Methodologies, Prediction Approaches, and Tools by Mohd Mustaqeem, Mahfooz Alam, Suhel Mustajab, Faisal Alshanketi, Shadab Alam, Mohammed Shuaib

“…The survey revealed that previous research often lacked appropriate datasets with the necessary characteristics and data validation methods. Additionally, many standard datasets suffer from a lack of labels, which hinders effective defect detection. …”
Get full text

Exploring corrosion protection of mild steel by ionic liquid functionalized graphene oxide: Gravimetric, electrochemical, and surface studies by M.Alharbi, Ruby Aslam, Ajahar Khan, Khalid A. Alamry, Mahmoud A. Hussein, Yas Al-Hadeethi, Elena Bekyarova, S. Alqahtani

“…The pronounced increases in polarization resistance (Rp) and reductions in corrosion current density (Icorr) indicated the strong adsorption of inhibitor molecules onto metal surface. In addition, the values of open circuit potential (OCP) and corrosion potential (Ecorr) were further evidence of a mixed inhibition mechanism. …”
Get full text

Experimental opisthorchiasis: a study of blood cells, hematopoiesis and startle reflex in laboratory animals by D. F. Avgustinovich, I. A. Orlovskaya, L. B. Toporkova, G. B. Vishnivetskaya, A. V. Katokhin, M. N. Lvova, E. V. Kashina, N. P. Bondar, N. A. Feofanova, V. A. Mordvinov

“…Infected hamsters had no reaction of habituation  to the sound stimulus. In addition, the maturation of O. felineus worms was faster in hamsters than in mice. …”
Get full text

Impact of Preoperative Anemia on Perioperative Outcomes in Patients Undergoing Elective Colorectal Surgery by Liu Liu, Lin Liu, Li-Chuang Liang, Zhi-qiang Zhu, Xiao Wan, Heng-bing Dai, Qiang Huang

“…Patients with colon cancer presented higher rate of POA than patients with rectal cancer (60% versus 40% for colon cancer versus rectal cancer). In addition, female patients and patients with large tumor mass (>4 cm) had a higher rate of POA than male patients and patients with small tumor (≤4 cm), respectively. …”
Get full text

Multiple gene-deletion vaccinia virus Tiantan strain against mpox by Yilong Zhu, Dapeng Li, Renshuang Zhao, Minghua Chen, Yue Li, Xia Yang, Hongyue Mao, Xiao Li, Yiquan Li, Chao Shang, Xianzhu Xia

“…The VTT-specific and neutralizing antibody titers, specific T cell levels, and degree of dendritic cell maturation of the mice, in addition to the MPXV neutralizing antibody titers and IFN-γ, IL-6, and TNF-α levels of the rabbits were markedly higher in the groups immunized with TTVAC7 and TTVC5 than the control groups (p < 0.05). …”
Get full text

A comparative study on spin-to-charge and charge-to-spin conversion using modulated Dirac surface states of Bi2Se3 by Youngmin Lee, Jonghoon Kim, Seungwon Rho, Seok-Bo Hong, Hyeongmun Kim, Jaehan Park, Dajung Kim, Chul Kang, Myung-Ho Bae, Mann-Ho Cho

“…Specifically, the interaction of d-orbital of the unbound hafnium in HfO2-x and Bi2Se3 enhances the spin-orbit interaction. In addition, we found that the complex interaction between the surface and bulk states affects the spin diffusion length and the spin current injection region. …”
Get full text

Optimizing network locations of weigh-in-motion stations: A multi-objective approach for enhancing infrastructure management by Chan Yang, Tu Lan, Patrick Lou, Hani Nassif, Kaan Ozbay, Chaekuk Na

“…This approach not only aids in long-term infrastructure monitoring aligned with NYCDOT’s goals but also supports future overweight enforcement efforts. Additionally, the study introduces an analytical framework to enhance the utilization of WIM data in analyzing truck load impacts.…”
Get full text

Mapping Variability of Mycotoxins in Individual Oat Kernels from Batch Samples: Implications for Sampling and Food Safety by Irene Teixido-Orries, Francisco Molino, Bianca Castro-Criado, Monika Jodkowska, Angel Medina, Sonia Marín, Carol Verheecke-Vaessen

“…Additionally, sampling statistics showed that as the percentage of selected kernels increased, the probability of batch sample rejection for DON contamination rose significantly. …”
Get full text

Updates on hosts and distribution of pepper yellow leaf curl Indonesia virus and squash leaf curl China virus in Central Java Province, Indonesia by Adyatma Irawan Santosa, Adenisa Hanifah Irbati, Geby Nandita Cahyaning Pratiwi, Antama Surwadinata, Putri Laeshita, Krisnanda Surya Dharma, Reko Saputra Jaya, Annisa Lisa Andriyani, Chilya Qurrota A'yun, Chusnu Naura Syifa Fatika

“…This study contributed additional knowledge on molecular variation while expanding hosts and distribution of PepYLCIV and SLCCNV in Central Java Province. …”
Get full text

DLBWE-Cys: a deep-learning-based tool for identifying cysteine S-carboxyethylation sites using binary-weight encoding by Zhengtao Luo, Zhengtao Luo, Zhengtao Luo, Qingyong Wang, Qingyong Wang, Qingyong Wang, Yingchun Xia, Yingchun Xia, Yingchun Xia, Xiaolei Zhu, Xiaolei Zhu, Xiaolei Zhu, Shuai Yang, Shuai Yang, Shuai Yang, Zhaochun Xu, Zhaochun Xu, Lichuan Gu, Lichuan Gu, Lichuan Gu

“…Feature comparison experiments confirmed the superiority of our proposed Binary-Weight encoding method over other encoding techniques. t-SNE visualization further validated the model’s effective classification capabilities. Additionally, we confirmed the similarity between the distribution of positional weights in our Binary-Weight encoding and the allocation of weights in attentional mechanisms. …”
Get full text

Optimization of In-Motion EV Charging Infrastructure for Power Systems Using Generative Adversarial Network-Based Distributionally Robust Techniques by Dong Hua, Peifeng Yan, Suisheng Liu, Qinglin Lin, Peiyi Cui, Qian Li

“…Dynamic charging enabled EV users to save USD 0.08 per kilometer through reduced stationary charging downtime, optimized travel efficiency, and lower energy costs. Additionally, the system minimizes maintenance costs by optimizing lane and grid reliability. …”
Get full text

2-O-β-D-Glucopyranosyl-L-ascorbic acid, an ascorbic acid derivative isolated from the fruits of Lycium barbarum L., ameliorates high fructose-induced neuroinflammation in mice: inv... by Wei Dong, Yujia Peng, Guijie Chen, Zhiyong Xie, Weiqi Xu, Wangting Zhou, Jia Mi, Lu Lu, Yi Sun, Xiaoxiong Zeng, Youlong Cao, Yamei Yan

“…These beneficial effects were transmissible by horizontal fecal microbiome transplantation, transferring from AA-2βG fed mice to HFrD fed mice. Additionally, AA-2βG exerted neuroprotective effects involving the enrichment of Lactobacillus and Akkermansia, potentially beneficial intestinal bacteria. …”
Get full text

Human TRMT1 and TRMT1L paralogs ensure the proper modification state, stability, and function of tRNAs by Kejia Zhang, Aidan C. Manning, Jenna M. Lentini, Jonathan Howard, Felix Dalwigk, Reza Maroofian, Stephanie Efthymiou, Patricia Chan, Sergei I. Eliseev, Zi Yang, Hayley Chang, Ehsan Ghayoor Karimiani, Behnoosh Bakhshoodeh, Henry Houlden, Stefanie M. Kaiser, Todd M. Lowe, Dragony Fu

“…Intriguingly, vertebrates encode an additional tRNA methyltransferase 1-like (TRMT1L) paralog. …”
Get full text

How Antiretroviral Drug Concentrations Could Be Affected by Oxidative Stress, Physical Capacities and Genetics: A Focus on Dolutegravir Treated Male PLWH by Jessica Cusato, Anna Mulasso, Micol Ferrara, Alessandra Manca, Guido Accardo, Alice Palermiti, Miriam Antonucci, Gianluca Bianco, Francesco Chiara, Jacopo Mula, Maria Cristina Tettoni, Simone Cuomo, Giulia Trevisan, Stefano Bonora, Giovanni Di Perri, Corrado Lupo, Alberto Rainoldi, Antonio D’Avolio

“…In addition, pharmacogenetics can influence transporter activity, and thus drug exposure. …”
Get full text

MicroRNA-129-3p Suppresses Tumor Progression and Chemoradioresistance in Head and Neck Squamous Cell Carcinoma by Tae Mi Yoon, Sun-Ae Kim, Eun Kyung Jung, Young-Kook Kim, Kyung-Hwa Lee, Sang Chul Lim

“…In this study, the role of miR-129-3p, the main mature form of miR-129, in tumor progression and chemoradiotherapy resistance in head and neck cancer was evaluated. (2) Methods: RT-PCR, western blotting, cell proliferation assays, cell apoptosis assays, and cell invasion and migration assays were used. (3) Results: In this study, the miR-129-3p overexpression suppressed the proliferation, invasion, and migration of SNU1041, SCC15, and SCC25 human HNSCC cell lines. Additionally, it induced apoptosis and enhanced radiation-/cisplatin-induced apoptosis of SNU1041, SCC15, and SCC25 cells. …”
Get full text

C. perfringens enterotoxin-claudin pore complex: Models for structure, mechanism of pore assembly and cation permeability by Santhosh Kumar Nagarajan, Joy Weber, Daniel Roderer, Jörg Piontek

“…The pore is lined with three hexa-glutamate rings, which differ from other β-PFTs and confer CPE-specific cation selectivity. Additionally, the pore center is indicated to be anchored by a dodecameric claudin ring formed by a cis-interaction variant of an interface found in claudin-based tight junction strands. …”
Get full text