Tear film lipid layer thickness and blink dynamics in patients with blepharospasm by Ji-Sun Paik, Min Jeong Kwon, Ga Hee Nam, Kyungdo Han, Woong-Joo Whang, Ho Sik Hwang, Suk-Woo Yang, Hyun-Seung Kim, Kyung Sun Na, Won-Kyung Cho

“…Abstract Background This study investigates the effect of botulinum toxin A on lipid layer thickness (LLT) and blink dynamics in patients with benign essential blepharospasm (BEB) compared to dry eye disease (DED) patients. Methods We reviewed the medical records of patients with dry eye disease (DED) and BEB treated with botulinum toxin A (BoT A) injections. …”
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Long-term maintenance of patient-specific characteristics in tumoroids from six cancer indications by Colin D. Paul, Chris Yankaskas, Pradip Shahi Thakuri, Brittany Balhouse, Shyanne Salen, Amber Bullock, Sylvia Beam, Anthony Chatman, Sybelle Djikeng, Xiaoyu Jenny Yang, Garrett Wong, Isha Dey, Spencer Holmes, Abigail Dockey, Lindsay Bailey-Steinitz, Lina Zheng, Weizhong Li, Vivek Chandra, Jakhan Nguyen, Jason Sharp, Erik Willems, Mark Kennedy, Matthew R. Dallas, David Kuninger

“…Previously established tumoroid lines were also transitioned to OncoPro medium and exhibited, on average, similar growth rates and conserved donor-specific characteristics when compared to original media systems. Additionally, OncoPro medium was compatible with both embedded culture in extracellular matrix and growth in a suspension format for facile culture and scale up. …”
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H-NS controls the susceptibility of Escherichia coli to aminoglycosides by interfering its uptake and efflux by Qiuru Chen, Yulei Liang, Yanbin Dong, Junling Cui, Kun He, Xiaoyuan Ma, Jinfeng Zhao, Jinfeng Zhao, Yajun Zhai, Yajun Zhai, Li Yuan, Li Yuan, Li Yuan, Li Yuan

“…Meanwhile, hns deletion also led to remarkable inhibition of the efflux pumps activity and decreased expressions of efflux-related genes clbM, acrA, acrB, acrD, and emrE, which reduced the efflux of aminoglycosides. In addition, the activation of glycolysis and electron transport chain, as well as the reduction of Δψ dissipation, could lead to a remarkable increase in proton motive force (PMF), thus further inducing more aminoglycosides uptake by strain EΔhns. …”
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DOCK4 Is a Platinum-Chemosensitive and Prognostic-Related Biomarker in Ovarian Cancer by Qianqian Zhao, Jie Zhong, Ping Lu, Xiao Feng, Ying Han, Chenqi Ling, Wenke Guo, Weijin Zhou, Fudong Yu

“…We further found that there were significant correlations between DOCK4 expression and the levels of CD4+ T cell infiltration, dendritic cell infiltration, and neutrophil infiltration in OV. In addition, we verified the expression level of DOCK4 in OV cell lines treated with platinum drugs and found that DOCK4 is potentially responsive to platinum drugs. …”
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Archival Research, Underwater Optical Surveys, and 3D Modelling: Three Stages for Shaping the Wreck of the Steamship <i>Bengala</i> (Isola di Capo Rizzuto, Crotone, Italy) by Salvatore Medaglia, Fabio Bruno, Ana Castelli, Matteo Collina, Barbara Davidde Petriaggi, Luca De Rosa, Julieta Frere, Fabrizio Fuoco, Guillermo Gutiérrez, Antonio Lagudi, Francesco Megna, Raffaele Peluso

“…The model can be used in augmented reality (AR) applications to enhance underwater exploration, allowing divers to visualise the reconstructed ship in its original environment. Additionally, it supports museum exhibits, interactive visualisations, and educational games, making it a valuable resource for engaging the public with maritime history and archaeology.…”
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A meta-core outcome set for stillbirth prevention and bereavement care following stillbirth in LMIC by Jamie J Kirkham, Declan Devane, Carol Bedwell, Tina Lavender, Tracey A Mills, Sabina Wakasiaka, Rose Laisser, Bellington Vwalika, Nasim Chaudhry, Angela Chimwaza, Elizabeth Ombeva Ayebare, Sudhindrashayana Fattepur, Tayyeba Kiran, Kushupika Dube, Farai Marenga, Idesi Chilinda, Unice Goshomi, Allen Nabisere, Zaib Un Nisa

“…For bereavement care following a stillbirth, five core outcomes reached a consensus, which included outcomes related to labour and birth, a postpartum complication, care experience, mental health and emotional and social well-being.Discussion These COS will improve the consistency of outcomes for future research in an LMIC setting. Additionally, they will complement existing COS for stillbirth prevention and bereavement care developed in high-income settings. …”
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Lyme disease associated neurological and musculoskeletal symptoms: A systematic review and meta-analysis by Ganesh Bushi, Ashok Kumar Balaraman, Shilpa Gaidhane, Suhas Ballal, Sanjay Kumar, Mahakshit Bhat, Shilpa Sharma, M Ravi Kumar, Aashna Sinha, Mahalaqua Nazli Khatib, Nishant Rai, Sanjit Sah, Ambanna Yappalparvi, Shailesh Kumar Samal, Doddolla Lingamaiah, Muhammed Shabil

“…Although heterogeneity across the studies was high, sensitivity analysis confirmed the consistency of our findings. Additionally, there was evidence of publication bias. …”
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Assisted Egg White Biogenic Synthesis for Elaboration of ZnO Nanoparticles by Elena Andreea Vijan, Ecaterina Magdalena Modan, Sorin Georgian Moga, Denis Aurelian Negrea, Adriana-Gabriela Schiopu, Mihai Oproescu, Daniela Istrate

“…These biomolecules interact with metal ions, leading to the reduction and nucleation of nanoparticles. Additionally, the proteins in egg white act as capping agents, stabilizing the nanoparticles and preventing their aggregation. …”
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FMT reduces systemic inflammatory response in severe acute pancreatitis by increasing the abundance of intestinal Bifidobacteria and fecal bacteria by Yanning Mao, Yandong Huang, Weiwei Zhang, Huiping Liang, Fengming Liu, Qi Luo, Chunqin Xu, Yi Qin, Jiawen Liu, Shaobo Tang, Huaying Liu, Xiaolong Ge

“…Receiver operating characteristic (ROC) curve analysis indicated that Bifidobacterium longum might play a critical role in the efficacy of FMT, with an area under the curve (AUC) value of 0.84. Additionally, there was a negative correlation between Bifidobacterium longum abundance and procalcitonin (PCT) levels, as well as a negative correlation between Escherichia coli abundance and both CT and Ca values (P < 0.05). …”
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Chronic NaAsO2 exposure promotes migration and invasion of prostate cancer cells by Akt/GSK-3β/β-catenin/TCF4 axis-mediated epithelial-mesenchymal transition by Zhi-Hui Zhang, Hai-Xin Yan, Ming-Dong Liu, Feng-Wen Niu, Kai Yao, Shi-Yao Feng, Xi Li, Yuan-Hua Chen, Dong-Dong Xie

“…Wound healing and Transwell assays showed that chronic NaAsO2 exposure promoted migration and invasion of DU145 and PC-3 cells. In addition, chronic NaAsO2 exposure induced epithelial-mesenchymal transition (EMT) of DU145 cells by promoting β-catenin/TCF4 transcriptional activity. …”
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Clinical findings of acute necrotizing esophagitis complicated by diabetic ketoacidosis by Yuichiro Iwamoto, Tomohiko Kimura, Takashi Itoh, Shigehito Mori, Taku Sasaki, Mana Ohnishi, Haruka Takenouchi, Hideyuki Iwamoto, Junpei Sanada, Yoshiro Fushimi, Yukino Katakura, Fuminori Tatsumi, Masashi Shimoda, Shuhei Nakanishi, Tomoatsu Mune, Kohei Kaku, Hideaki Kaneto

“…Notably, ethanol intake was significantly higher in the ANE group (44.4 ± 25.4 g/day) compared to the non-ANE group (6.8 ± 14.0 g/day; p = 0.013). Additionally, sodium-glucose transport protein 2 inhibitor use was significantly more prevalent in the ANE group (p = 0.013). …”
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Description of a new Ornithodoros (Pavlovskyella) (Ixodida: Argasidae) tick species from Pakistan by Abid Ali, Mehran Khan, Muhammad Numan, Abdulaziz Alouffi, Mashal M. Almutairi, Ronel Pienaar, Minique H. de Castro, Lidia Chitimia-Dobler, Sebastián Muñoz-Leal, Ben J. Mans, Ala Tabor

“…., which were collected from animal shelters in Khyber Pakhtunkhwa, Pakistan. Providing additional collections from that same locality and a comprehensive analysis involving detailed morphological and mitogenome-based comparisons with closely related species, this study formally designates a novel species for these specimens. …”
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Incidence and Factors Associated with Postoperative Delayed Hyponatremia after Transsphenoidal Pituitary Surgery: A Meta-Analysis and Systematic Review by Cheng-Chi Lee, Yu-Chi Wang, Yu-Tse Liu, Yin-Cheng Huang, Peng-Wei Hsu, Kuo-Chen Wei, Ko-Ting Chen, Ya-Jui Lin, Chi-Cheng Chuang

“…Among the predictors evaluated, older age was the only significant factor associated with increased delayed hyponatremia (odds ratio = 1.16, 95% CI = 1.04–1.29, P = 0.006). …”
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Conception of a mobile health application targeting early postoperative physiotherapeutic care after total knee replacement, a qualitative study by Hassan Tarek Hakam, Hassan Tarek Hakam, Hassan Tarek Hakam, Nikolai Ramadanov, Nikolai Ramadanov, Awda Garzuzi, Mikhail Salzmann, Mikhail Salzmann, Hannes Hofmann, Hannes Hofmann, Felix Muehlensiepen, Felix Muehlensiepen, Roland Becker, Roland Becker, Robert Prill, Robert Prill, Robert Prill

“…Primarily, mobile applications targeting rehabilitative care after TKR need to be tailored to the needs of the elderly population. Additionally, no unified rehabilitative physiotherapeutic (PT) program was found reflecting a discrepancy regarding what exercises are most useful. …”
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Effects of non-contact electric fields on the kidneys and livers of tumour-bearing rats [version 6; peer review: 1 approved, 2 approved with reservations, 1 not approved] by Luthfi Nurhidayat, Ahmad Ghitha Fadhlurrahman, Firman Alamsyah, Warsito Purwo Taruno, Nisrina Firdausi, Subekti Evi Dwi Nugraheni, Rarastoeti Pratiwi

“…Animals were treated with 7,12-dimethylbenz[a]anthracene at a dose of 20 mg/kg body weight for mammary tumour induction. Animals were then exposed to electric fields (100 kHz, 50-60 V/m) for 10 hours a day for three weeks. …”
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Ampelopsin induces MDA-MB-231 cell cycle arrest through cyclin B1-mediated PI3K/AKT/mTOR pathway in vitro and in vivo by Meng Minjun, Yang Qiaolu, Ouyang Zhong, Yang Qingmo, Wu Xinyi, Huang Yufan, Su Yonghui, Chen Shuanglong, Chen Wenlin

“…In the present work, the results showed that AMP could significantly inhibit the growth of breast cancer MDA-MB-231 cells in vitro and in vivo. In addition, treatment with AMP decreased the levels of PI3K, AKT and mTOR, as well as cyclin B1 expression, followed by p53/p21 pathway activation to arrest the cell cycle at G2/M. …”
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IFN-γ/TNF-α Synergism Induces Pro-Inflammatory Cytokine and Chemokine Production by In Vitro Canine Keratinocytes by Kyungsook Jung, Ji-Yeong Ku, Je-Seong Kwon, Gayeon Won, Hakyoung Yoon, Sang-Ik Oh, Mi Hye Kim, Chongchan Kim, Ji-Seon Yoon

“…CPEKs incubated with the IFN-γ/TNF-α mixture showed significantly increased IL-6 concentration. In addition, significantly increased concentrations of CXCL8 were detected in CPEKs incubated with TNF-α and with the IFN-γ/TNF-α mixture. …”
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Screening of Lactic Acid Bacteria Isolated from Fermented Cowpea and Optimization of Biomass Production Conditions by Hong Xu, Danyang Li, Xue Jiang, Qi Pei, Zhengqin Li, Philippe Madjirebaye, Mingyong Xie, Tao Xiong, Zhanggen Liu

“…Using these response surface plots and point predictions, the optimal factors were soy peptone (38.75 g/L), FeSO₄ (0.10 g/L), and VB₇ (20 g/L). In addition, the optimized incubation conditions were a temperature of 39 °C, an initial pH value of 7, and an inoculation volume of 3%. …”
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A survey of team culture and learning organization in the resuscitation of neonates with congenital anomalies: A single center experience by Anna Bostwick, Anne Ades, Carolina Rodriguez-Paras, Madeline Dombroski, Charis Lim, Maria Ordoñez Paredes, Lauren Heimall, Leane Soorikian, Sara C. Handley, Heidi M. Herrick

“…Methods: We administered the Short-Form Learning Organization Survey with the addition of an open-ended question to all delivery room resuscitation team members from 5/2023 to 7/2023. …”
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Toward enhancement of antibody thermostability and affinity by computational design in the absence of antigen by Mark Hutchinson, Jeffrey A. Ruffolo, Nantaporn Haskins, Michael Iannotti, Giuliana Vozza, Tony Pham, Nurjahan Mehzabeen, Harini Shandilya, Keith Rickert, Rebecca Croasdale-Wood, Melissa Damschroder, Ying Fu, Andrew Dippel, Jeffrey J. Gray, Gilad Kaplan

“…The 200 variants were produced through a robust high-throughput method and tested for thermal and colloidal stability (Tonset, Tm, Tagg), affinity (KD) relative to the parental antibody, and for developability parameters (nonspecific binding, aggregation propensity, self-association). …”
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