Protocols for the application of human embryonic stem cell-derived neurons for aging modeling and gene manipulation by Hui Zhang, Shuhui Sun, Juan Carlos Izpisua Belmonte, Guang-Hui Liu, Si Wang, Weiqi Zhang, Jing Qu

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Protocol for semisynthesis of histone H4 with site-specific modifications using irreversible sortase-mediated ligation by Yihang Xiao, Kun Zou, Mingxuan Wu

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Protocol for isolation of human bone marrow stromal cells and characterization of cellular metabolism by Martina Dzubanova, Michaela Ferencakova, Andrea Benova, Dalia Ali, Michaela Tencerova

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Comprehensive guide for epigenetics and transcriptomics data quality control by Arianna Comendul, Frederique Ruf-Zamojski, Colby T. Ford, Pankaj Agarwal, Elena Zaslavsky, German Nudelman, Manoj Hariharan, Aliza Rubenstein, Hanna Pincas, Venugopalan D. Nair, Adam M. Michaleas, Philip D. Fremont-Smith, Darrell O. Ricke, Stuart C. Sealfon, Christopher W. Woods, Kajal T. Claypool, Rafael Jaimes, III

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Protocol for profiling RNA m5C methylation at base resolution using m5C-TAC-seq by Xiaoting Zhang, Liang Lu, Chengqi Yi, Xiaoyu Li

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Protocol for the preparation of zebrafish whole heart cell suspension for single-cell analyses by Karim Abu Nahia, Cecilia Lanny Winata

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Protocol to study the role of medial entorhinal cortex-basolateral amygdala circuit in context-induced retrieval of morphine withdrawal memory in mice by Yali Fu, Ting Ye, Ming Chen, Bin Lai, Ping Zheng

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Protocol for fecal microbiota transplantation: A microaerophilic approach for mice housed in a specific pathogen-free facility by Shari Wouters, Hugo Moors, Mieke Verslegers, Natalie Leys, Surbhi Malhotra-Kumar, Samir Kumar-Singh, Mohamed Mysara

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Protocol for the isolation of brain microvessels and visualization of RNA fluorescence in mice and humans by Olivia M. Osborne, Oandy Naranjo, Silvia Torices, Sarah Schmidlin, Destiny Tiburcio, Minseon Park, Michal Toborek

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Protocol for the isolation of silk glands from silkworms for snRNA-seq and spatial transcriptomics by Yan Ma, Keshu Dong, Jie Hu, Yiyun Tang, Hanfu Xu

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Protocol for estimating the contrast transfer function and absolute tilt angle offset for cryo-electron tomography using CTFMeasure by Ranhao Zhang, Yuan Shen, Xueming Li

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Protocol for high-yield bacterial expression and purification of the voltage-dependent anion channel 1 for high-throughput biophysical assays by Stefano Conti Nibali, Andrea Magrì, Angela Messina, Armin Wagner, Ramona Duman, Vito De Pinto, Cristian Turato, Cristina Arrigoni, Marco Lolicato

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Protocol for extracellular vesicle secretion-related gene screening via ExoScreen technique by Tomofumi Yamamoto, Fumihiko Urabe, Yusuke Yoshioka, Yusuke Yamamoto, Takahiro Ochiya

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Protocol to assess changes in brain network resistance to perturbation during offline processing using TMS-EEG by Martina Bracco, Tuomas P. Mutanen, Domenica Veniero, Gregor Thut, Edwin M. Robertson

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Protocol for a patient-derived preclinical platform to model tumor-immune interactions and evaluate therapeutic efficacy by Wen-Hsin Chang, Andrew I. Chin, Ching-Hsien Chen

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Protocol for live imaging of axonal transport in iPSC-derived iNeurons by Dan Dou, Erika L.F. Holzbaur, C. Alexander Boecker

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A protocol for assessing the adhesion potential of lactic acid bacteria on various cell types by Lakshmi Jayaram, Ramith Ramu, Deepthi Puttegowda, Jayanthi M K, Chandana Kumari V B, Khang Wen Goh

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Protocol for generating human assembloids to investigate thalamocortical and corticothalamic synaptic transmission and plasticity by Anjana Nityanandam, Mary H. Patton, Ildar T. Bayazitov, Kyle D. Newman, Kristen T. Thomas, Stanislav S. Zakharenko

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Protocol for cell image-based spatiotemporal proteomics in budding yeast by Athanasios Litsios, Myra Paz David Masinas, Helena Friesen, Charles Boone, Brenda J. Andrews

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Protocol for perfusing human axillary lymph nodes ex vivo to study structure and function in real time by Amy Llewellyn, Rachel Barrow-McGee, Julia Stevenson, Jasmine Gore, Kalnisha Naidoo

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