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  1. 541
    Protocol for profiling RNA m5C methylation at base resolution using m5C-TAC-seq

    Protocol for profiling RNA m5C methylation at base resolution using m5C-TAC-seq by Xiaoting Zhang, Liang Lu, Chengqi Yi, Xiaoyu Li

    Published 2025-03-01
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  2. 542
    Protocol for the preparation of zebrafish whole heart cell suspension for single-cell analyses

    Protocol for the preparation of zebrafish whole heart cell suspension for single-cell analyses by Karim Abu Nahia, Cecilia Lanny Winata

    Published 2025-03-01
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  3. 543
    Protocol to study the role of medial entorhinal cortex-basolateral amygdala circuit in context-induced retrieval of morphine withdrawal memory in mice

    Protocol to study the role of medial entorhinal cortex-basolateral amygdala circuit in context-induced retrieval of morphine withdrawal memory in mice by Yali Fu, Ting Ye, Ming Chen, Bin Lai, Ping Zheng

    Published 2025-03-01
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  4. 544
    Protocol for estimating the contrast transfer function and absolute tilt angle offset for cryo-electron tomography using CTFMeasure

    Protocol for estimating the contrast transfer function and absolute tilt angle offset for cryo-electron tomography using CTFMeasure by Ranhao Zhang, Yuan Shen, Xueming Li

    Published 2025-03-01
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  5. 545
    Protocol to assess changes in brain network resistance to perturbation during offline processing using TMS-EEG

    Protocol to assess changes in brain network resistance to perturbation during offline processing using TMS-EEG by Martina Bracco, Tuomas P. Mutanen, Domenica Veniero, Gregor Thut, Edwin M. Robertson

    Published 2025-03-01
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  6. 546
    Protocol for a patient-derived preclinical platform to model tumor-immune interactions and evaluate therapeutic efficacy

    Protocol for a patient-derived preclinical platform to model tumor-immune interactions and evaluate therapeutic efficacy by Wen-Hsin Chang, Andrew I. Chin, Ching-Hsien Chen

    Published 2025-03-01
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  7. 547
    A protocol for assessing the adhesion potential of lactic acid bacteria on various cell types

    A protocol for assessing the adhesion potential of lactic acid bacteria on various cell types by Lakshmi Jayaram, Ramith Ramu, Deepthi Puttegowda, Jayanthi M K, Chandana Kumari V B, Khang Wen Goh

    Published 2025-03-01
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  8. 548
    Protocol for generating human assembloids to investigate thalamocortical and corticothalamic synaptic transmission and plasticity

    Protocol for generating human assembloids to investigate thalamocortical and corticothalamic synaptic transmission and plasticity by Anjana Nityanandam, Mary H. Patton, Ildar T. Bayazitov, Kyle D. Newman, Kristen T. Thomas, Stanislav S. Zakharenko

    Published 2025-03-01
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  9. 549
    Protocol for cell image-based spatiotemporal proteomics in budding yeast

    Protocol for cell image-based spatiotemporal proteomics in budding yeast by Athanasios Litsios, Myra Paz David Masinas, Helena Friesen, Charles Boone, Brenda J. Andrews

    Published 2025-03-01
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  10. 550
    Protocol for perfusing human axillary lymph nodes ex vivo to study structure and function in real time

    Protocol for perfusing human axillary lymph nodes ex vivo to study structure and function in real time by Amy Llewellyn, Rachel Barrow-McGee, Julia Stevenson, Jasmine Gore, Kalnisha Naidoo

    Published 2025-03-01
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  11. 551
    Protocol for the quantitative detection of mtDNA in the supernatants of activated human naive CD8+ T cells

    Protocol for the quantitative detection of mtDNA in the supernatants of activated human naive CD8+ T cells by Linlin Li, Jun Jin

    Published 2025-03-01
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  12. 552
    Protocol for in vivo chromatin immunoprecipitation on purified chromatin isolated from mouse liver nuclei

    Protocol for in vivo chromatin immunoprecipitation on purified chromatin isolated from mouse liver nuclei by Lei Li, May G. Akl, Scott B. Widenmaier

    Published 2025-03-01
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  13. 553
    Protocol to boost the robustness and accuracy of spatial transcriptomics algorithms using ensemble techniques

    Protocol to boost the robustness and accuracy of spatial transcriptomics algorithms using ensemble techniques by Jiazhang Cai, Shushan Wu, Huimin Cheng, Wenxuan Zhong, Guo-Cheng Yuan, Ping Ma

    Published 2025-03-01
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  14. 554
    Protocol for the visualization of pRps6-positive cells in larval zebrafish brains using whole-mount immunofluorescence and light-sheet microscopy

    Protocol for the visualization of pRps6-positive cells in larval zebrafish brains using whole-mount immunofluorescence and light-sheet microscopy by Olga Doszyn, Tomasz Dulski, Justyna Zmorzynska

    Published 2025-03-01
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  15. 555
    Protocol for the generation of HLF+ HOXA+ human hematopoietic progenitor cells from pluripotent stem cells

    Protocol for the generation of HLF+ HOXA+ human hematopoietic progenitor cells from pluripotent stem cells by Sherry Li Zheng, Jonas L. Fowler, Julie Y. Chen, Christopher Li, Elaine Lin, Alana T. Nguyen, Angela Chen, George Q. Daley, Lay Teng Ang, Kyle M. Loh

    Published 2025-03-01
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  16. 556
    Protocol for recombinant rabies virus titration by quantitative PCR

    Protocol for recombinant rabies virus titration by quantitative PCR by He Zhang, Xueping Gao, Xiangyu Ge, Xiao Wang, Minghui Song, Xia Zhang, Lei Jin

    Published 2025-03-01
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  17. 557
    A 2D cell segmentation protocol for monitoring multiple STAT signaling pathways by fluorescence microscopy

    A 2D cell segmentation protocol for monitoring multiple STAT signaling pathways by fluorescence microscopy by Gergő Szanda, Éva Wisniewski, László Barna, Gábor Turu, Ken Mackie

    Published 2025-03-01
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  18. 558
    Protocol for multiplexed RNAscope-based imaging of mRNAs in whole-mount adult Drosophila brains

    Protocol for multiplexed RNAscope-based imaging of mRNAs in whole-mount adult Drosophila brains by Meilin Wu, Vanessa Lambatan, Peng Guo, William J. Joiner

    Published 2025-03-01
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  19. 559
    Protocol for real-time assessment of energy metabolism in dissociated mouse retinal photoreceptors using a SeahorseXFe24 analyzer

    Protocol for real-time assessment of energy metabolism in dissociated mouse retinal photoreceptors using a SeahorseXFe24 analyzer by Maria Yera, Joshua J. Wang, Sarah X. Zhang

    Published 2025-03-01
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  20. 560
    Protocol to image, segment, and quantify cortical contractions in maturing mouse oocytes

    Protocol to image, segment, and quantify cortical contractions in maturing mouse oocytes by Gaëlle Letort, Elvira Nikalayevich

    Published 2025-03-01
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