Ginger-Derived Exosome-Like Nanoparticles Loaded With Indocyanine Green Enhances Phototherapy Efficacy for Breast Cancer by Guo Z, Li G, Shen L, Pan J, Dou D, Gong Y, Shi W, Sun Y, Zhang Y, Ma K, Cui C, Li W, Liu Q, Zhu X

“…Moreover, by detecting the expression of CD31, N-cadherin, IL-6, IFN-γ, CD8, p16, p21, and p53 in tumor tissues, we found that GDNPs@ICG substantially reduced angiogenesis, inhibited metastasis, activated the anti-tumor immune response, and promoted cell senescence in breast tumor.Conclusion: Our study demonstrated that the novel bio-nanoplatform GDNPs@ICG enhanced the photo-mediated therapeutic effect in breast tumor. …”
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CAR-CIK vs. CAR-T: benchmarking novel cytokine-induced killer cells as solid tumor immunotherapy in ErbB2+ rhabdomyosarcoma by Laura M. Moser, Laura M. Moser, Laura M. Moser, Laura M. Moser, Laura M. Moser, Catrin Heim, Sebastian E. Koschade, Sebastian E. Koschade, Sebastian E. Koschade, Sebastian E. Koschade, Sebastian E. Koschade, Philipp Wendel, Philipp Wendel, Philipp Wendel, Philipp Wendel, Süleyman Bozkurt, Sabine Harenkamp, Hermann Kreyenberg, Hermann Kreyenberg, Michael Merker, Michael Merker, Christian Münch, Christian Münch, Christian Münch, Elise Gradhand, Meike Vogler, Meike Vogler, Meike Vogler, Evelyn Ullrich, Evelyn Ullrich, Evelyn Ullrich, Evelyn Ullrich, Evelyn Ullrich, Halvard Bönig, Halvard Bönig, Jan-Henning Klusmann, Jan-Henning Klusmann, Jan-Henning Klusmann, Jan-Henning Klusmann, Peter Bader, Peter Bader, Peter Bader, Peter Bader, Peter Bader, Winfried S. Wels, Winfried S. Wels, Winfried S. Wels, Eva Rettinger, Eva Rettinger, Eva Rettinger, Eva Rettinger, Eva Rettinger

“…CAR-CIK cell cytokine signature showed significantly increased secretion of effector molecules like interferon-γ, perforin and granulysin, and lower secretion of Th2 cytokines IL-2, IL-4 and IL-10. Whole cell proteomics showed corresponding upregulation of chemokine signaling and NK-cytotoxicity pathways in CAR-CIK cells. …”
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Cybersicurezza e protezione dati: un rapporto ambivalente by Federica Resta

“…Il saggio analizza il rapporto tra protezione dati e cybersicurezza, alla luce dell’evoluzione che lo ha caratterizzato. …”
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The Role of Dendritic Cells in Adaptive Immune Response Induced by OVA/PDDA Nanoparticles by Daniele R. Pereira, Yunys Pérez-Betancourt, Bianca C. L. F. Távora, Geraldo S. Magalhães, Ana Maria Carmona-Ribeiro, Eliana L. Faquim-Mauro

“…Here, we aimed to evaluate the involvement of DCs in the immune response induced by OVA/PDDA. <b>Methods</b>: In vivo experiments were used to assess the ability of OVA/PDDA-NPs to induce anti-OVA antibodies by ELISA, as well as plasma cells and memory B cells using flow cytometry. …”
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Downregulation of TNF-α/TNF-R1 Signals by AT-Lipoxin A4 May Be a Significant Mechanism of Attenuation in SAP-Associated Lung Injury by Suhui Yu, Jianming Xie, Yukai Xiang, Shengjie Dai, Dinglai Yu, Hongwei Sun, Bicheng Chen, Mengtao Zhou

“…Moreover, AT-Lipoxin A4 inhibited downstream signals activated by TNF-α, including NF-κB/p65, JNK/MAPK, and ERK/MAPK. In vivo, AT-Lipoxin A4 significantly decreased pathological scores of the pancreas and lungs and the serum levels of IL-6 and TNF-α. …”
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Inhibitory Effect of Natural Anti-Inflammatory Compounds on Cytokines Released by Chronic Venous Disease Patient-Derived Endothelial Cells by Veronica Tisato, Giorgio Zauli, Erika Rimondi, Sergio Gianesini, Laura Brunelli, Erica Menegatti, Paolo Zamboni, Paola Secchiero

“…Among the cytokines/chemokines released by VEC, which recapitulated the systemic profile (IL-8, TNF-α, GM-CSF, INF-α2, G-CSF, MIP-1β, VEGF, EGF, Eotaxin, MCP-1, CXCL10, PDGF, and RANTES), we identified those targeted by ex vivo treatment with α-Lipoic acid and/or Ginkgoselect phytosome (GM-CSF, G-CSF, CXCL10, PDGF, and RANTES). …”
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MMP9-Associated Tumor Stem Cells, CCL1-Silenced Dendritic Cells, and Cytokine-Induced Killer Cells Have a Remarkable Therapeutic Efficacy for Acute Myeloid Leukemia by Activating T... by Min Dong, Guozhen Zhang, Jie Meng, Biou Liu, Duanfeng Jiang, Feng Liu

“…Meanwhile, blockage of MMP9 and CCL1 in DC-CIK cells dramatically increased IL-2 and IFN-γ, increased CD107aþ (LAMP-1) and granzyme B (GZMB), and downregulated PD-1, CTLA4, TIM3, and LAG3 T cells from AML patients and AML model mice. …”
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Phytochemicals for mitigating the COVID-19 crisis: evidence from pre-clinical and clinical studies by Atri Das, Swarnali Khan, Syamal Roy, Shantanabha Das

“…This review article has documented in vitro, in vivo, and clinical studies encompassing 28 different phytochemicals belonging to various chemical groups (e.g., polyphenols, alkaloids, terpenes) that show anti-COVID-19 activity. …”
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Baicalin Liposome Alleviates Lipopolysaccharide-Induced Acute Lung Injury in Mice via Inhibiting TLR4/JNK/ERK/NF-κB Pathway by Yu Long, Yan Xiang, Songyu Liu, Yulu Zhang, Jinyan Wan, Qiyue Yang, Mingquan Cui, Zhimin Ci, Nan Li, Wei Peng

“…In pharmacokinetics study, the values of t1/2 and AUC0-t in the BA-LP group were significantly higher than that of the BA group in normal mice, indicating that BA-LP could prolong the duration time in vivo of BA. The BA-LP group also showed a higher concentration in lung tissues than the BA group. …”
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Large-scale manufacturing and characterization of CMV-CD19CAR T cells by Stephen J Forman, Xiuli Wang, Ryan Urak, Miriam Walter, Min Guan, Tianxu Han, Vibhuti Vyas, Sheng-Hsuan Chien, Brenna Gittins, Mary C Clark, Sally Mokhtari, Angelo Cardoso, Don J Diamond, John Zaia, Ryotaro Nakamura

“…Resulting CMV-specific T cells were transduced with a lentiviral vector encoding a second generation CD19R:CD28:ζ/EGFRt CAR and expanded with interleukin 2 (IL-2) and IL-15 for 15 days before characterization.Results CMV-specific T cells were enriched from 0.8%±0.5 of input PBMC to 76.3%±11.6 in nine full-scale qualification runs (absolute yield of 4.2±3.3×106 IFNγ+T cells from an input of 1×109 PBMCs). …”
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A self-activated and protective module enhances the preclinical performance of allogeneic anti-CD70 CAR-T cells by Zhao Zhang, Lianfeng Zhao, Tinghui Huang, Zhengliang Chen, Zhengliang Chen, Yaoyao Zhao, Yaoyao Zhao, Junqing Liang, Xudong Ao, Xiaoqiong Jia, Lei Kang, Linghui Kong, Qi Jing, Jianhua Hu, Lili Gu, Feiyan Pan, Zhigang Hu, Lingfeng He, Muya Zhou, Jiannan Chen, Zhigang Guo

“…The SAP module consists of the CD47 extracellular domain, a mutant interleukin 7 receptor alpha (IL7Rα) transmembrane domain, and the IL7Rα intracellular domain, designed to protect UCAR-T cells from host immune attacks and enhance their survival. …”
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Una proposta di studio dei concetti di cybersicurezza e cyberresilienza in senso giuridico tra ordinamento europeo e italiano by Milena Antonella Rizzi, Federico Serini

“…Si tratta infatti di formulazioni composte da concetti che, se da una parte, come nel caso della sicurezza e della resilienza, sono di difficile concettualizzazione in senso legale, dall’altra, per quanto riguarda il cyberspazio, sono perfino inediti per l’ordinamento. …”
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L’identità e l’identificazione digitale del minore tra normativa nazionale e internazionale e i provvedimenti delle autorità competenti by Francesca Ricciulli

“…Il contributo, prendendo le mosse dallo schema di regolamento licenziato dall’AGCOM all’esito della consultazione sugli strumenti di age assurance avviata in attuazione del c.d. …”
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Autonomia e controllo dei minori on line. I nuovi confini della responsabilità genitoriale by Benedetta Agostinelli

“…La responsabilità genitoriale, che ha superato la potestà, è situazione giuridica complessa idonea a riassumere i doveri, gli obblighi e i diritti derivanti per il genitore dalla filiazione, tra i quali emerge quello di educare. …”
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Preclinical development of a standardized extract of Ilex paraguariensis A.St.-Hil for the treatment of obesity and metabolic syndrome by Sara E.L. Tolouei, Rodrigo Marcon, Fabiana Cardoso Vilela, Cristina Setim Freitas, Melina Heller, Edineia Lemos de Andrade, Sergio José Macedo Júnior, Adara Áurea dos Santos, Ruth Fernandes Rocha, Guilherme Pasetto Fadanni, Naiani Ferreira Marques, Jarbas Mota Siqueira Júnior, João B. Calixto

“…Finally, TI-076 did not interact with CYP3A4 in vivo and proved to be safe. …”
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Neoantigen-specific stimulation of tumor-infiltrating lymphocytes enables effective TCR isolation and expansion while preserving stem-like memory phenotypes by Yong Li, Steven A Rosenberg, Paul F Robbins, Zhiya Yu, Maria Parkhurst, Billel Gasmi, Nikolaos Zacharakis, Todd D Prickett, Jared J Gartner, Sri Krishna, Stephanie L Goff, Satyajit Ray, Frank J Lowery, Sivasish Sindiri, Alakesh Bera, Noam Levin, Sanghyun P Kim, Charles A Marquardt, Nolan R Vale, Lior Levy, Tiffany Benzine, Robert V Masi, Rafiqul Islam

“…Single-cell transcriptome analysis revealed that neoantigenic stimulation increased neoantigen-reactive TILs with stem-like memory phenotypes expressing IL-7R, CD62L, and KLF2. Furthermore, neoantigenic stimulation improved the in vivo antitumor efficacy of TILs relative to the conventional OKT3-induced rapid TIL expansion in p53-mutated or KRAS-mutated xenograft mouse models.Conclusions Taken together, neoantigenic stimulation of TILs selectively expands neoantigen-reactive TILs by frequencies and by their clonal repertoire. …”
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A strategy for selective screening of dual-target bioactive compounds against hypertrophic scar through inhibiting angiotensin II type 1 receptor while stimulating type 2 receptor... by Lejing Qu, Meizhi Jiao, Zilong Zhang, Yuanyuan Ou, Xue Zhao, Yajun Zhang, Xinfeng Zhao

“…Their functions on AT1R and AT2R expressions were investigated by in vitro and in vivo experiments. Results Aloe-emodin and emodin were identified as the potential bioactive compounds binding to both of the two receptors, thereby improving the appearance and pathomorphology of hypertrophic scar. …”
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Murine DX5+NKT Cells Display Their Cytotoxic and Proapoptotic Potentials against Colitis-Inducing CD4+CD62Lhigh T Cells through Fas Ligand by Jens M. Werner, Michael Damian, Stefan A. Farkas, Hans J. Schlitt, Edward K. Geissler, Matthias Hornung

“…As previously shown in vivo, DX5+NKT cells inhibit proliferation of CD4+CD62Lhigh cells in vitro after 96 h coculture compared to a CD4+CD62Lhigh monoculture (proliferation index: 1.39 ± 0.07 vs. 1.76 ± 0.12; P=0.0079). …”
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STING agonist-based treatment promotes vascular normalization and tertiary lymphoid structure formation in the therapeutic melanoma microenvironment by Walter J Storkus, Ronald J Fecek, Manoj Chelvanambi, Jennifer L Taylor

“…Consistent with a central role for DC in TLS formation, ADU S-100-activated mCD11c+ DCs also exhibited upregulated expression of TLS promoting factors including lymphotoxin-α (LTA), interleukin (IL)-36, inflammatory chemokines and type I interferons in vitro and in vivo. …”
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Periplanta americana extract regulates the Th17/Treg cell balance via Notch1 in ulcerative colitis by Yanqiu Zheng, Yanqiu Zheng, Huibiao Li, Shiyu Qi, Fan Xiao, Jinbin Song, Shiyin Liu, Xinlin Chen, Yanwu Li, Muyuan Chen

“…Network pharmacological analysis showed that the targets of UC intervention by PAE may be closely related to Th17 cell differentiation, the IL-17 signaling pathway, and cytokine-cytokine receptor interaction. …”
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