Development of SacB-based counterselection for efficient allelic exchange in Fusobacterium nucleatum by Peng Zhou, Bibek G C, Bo Hu, Chenggang Wu

“…Of these, counterselection-based unmarked in-frame mutation methods have proved notably effective. Under suitable growth conditions, cells carrying a counterselectable gene die, enabling efficient selection of rare, defined allelic exchange mutants. …”
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IL-2-armored peptide-major histocompatibility class I bispecific antibodies redirect antiviral effector memory CD8+ T cells to induce potent anti-cancer cytotoxic activity with lim... by John S. Schardt, Even Walseng, Kim Le, Chunning Yang, Pooja Shah, Ying Fu, Kausar Alam, Cathryn R. Kelton, Yu Gu, Fengying Huang, Jia Lin, Wenhai Liu, Andrew Dippel, Hanzhi Zhang, Kathy Mulgrew, Stacy Pryts, Vijaykumar Chennupati, Hung-Chang Chen, Jessica Denham, Xiaoru Chen, Pallab Pradhan, Yuling Wu, Colin Hardman, Chihao Zhao, Michael Kierny, Yang Song, Simon J. Dovedi, Saso Cemerski, Yariv Mazor

“…Using an anti-epidermal growth factor receptor (EGFR) arm as a proof-of-concept, tumor-associated antigen paired with a single-chain HLA-A *02:01/CMVpp65 pMHC fusion moiety, we demonstrate in vitro that the IL-2-armored GPS modality robustly expands CMVpp65-specific CD8+ effector memory T cells and induces potent cytotoxic activity against target cancer cells. …”
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Response of Soil Respiration to Snowfall in a Kubuqi Salix Plantation Forest of During Freeze-thaw Period by WANG Jixuan, LAN Xiaozhen, PEI Zhiyong, ZHANG Junyao, WANG Xinping, LI Ying, WANG Haichao, SUN Xiaotian, SUN Kai

“…[Conclusion] Snowfall in winter can provide water for vegetation growth in early spring, and it regulates the carbon budget in arid and semi-arid regions; stubble levelling and land preparation can impede the release of carbon during the freeze-thaw period in arid and semi-arid areas and indirectly slow down the loss of carbon from the soil, thus contributing to the regulation of the carbon budget of the ecosystem.…”
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Identification and functional validation of a new gene conferring resistance to Soybean Mosaic Virus strains SC4 and SC20 in soybean by Muhammad Muzzafar Raza, Huiying Jia, Muhammad Khuram Razzaq, Bowen Li, Kai Li, Junyi Gai

“…Soybean Mosaic Virus (SMV) poses a serious threat to soybean production, often resulting in considerable yield losses or complete crop failure, particularly if infection occurs during early growth stages. While several SMV resistance genes have been identified, the genetic basis of resistance to certain strains remains poorly understood. …”
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Carbon Stock Estimation in the South Coastal Rehabilitation Area of Java Island by Budiadi Budiadi

“…High variation of tree growth and density were found, especially in mangrove stands, with a low level of natural regeneration. …”
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Evaluation of angular and positional changes of maxillary third molars following premolar extraction, molar distalization, and non-extraction treatments by Zeynep Çoban Büyükbayraktar, Hasan Camcı, Sultan İçmen, Artun Yangın

“…Descriptive data, including growth pattern, crowding, overjet, overbite, and maxillary incisor inclination, were recorded. …”
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Group a Streptococcus remains viable inside fibrin clots and gains access to human plasminogen for subsequent fibrinolysis and dissemination by Henry M. Vu, Thomas E. Moran, Zhong Liang, Yun-Juan Bao, Paulina G. Carles, Jessica C. Keane, Madelyn G. Cerney, Caitlyn N. Dahnke, Ana L. Flores-Mireles, Victoria A. Ploplis, Francis J. Castellino, Shaun W. Lee

“…Our findings reveal for the first time that GAS can engage a latent, growth-suspended phase whereby physical structures such as fibrin clots that immobilize an invading pathogen allow bacteria to remain viable until sufficient access to plasminogen allows it to initiate fibrinolysis and escape into surrounding blood system and tissues.IMPORTANCEGroup A Streptococcus (GAS) is a human-specific bacterial pathogen that causes infections ranging in severity from mild to severe infections that can often be fatal. …”
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Effect of radiotherapy exposure on fruquintinib plus sintilimab treatment in refractory microsatellite stable metastatic colorectal cancer: a prospective observation study by Jing Wang, Tao Zhang, Lei Zhao, Min Jin, Pindong Li, Shengli Yang, Hong Ma, Zhenyu Lin, Junli Liu, Hongli Liu, Kunyu Yang, Dandan Yu, Jianli Hu, Jun Xue, Mingxia Cheng, Chuying Huang

“…This study aimed to investigate the association between RT exposure and clinical responses to fruquintinib (a highly selective tyrosine kinase inhibitor of vascular endothelial growth factor receptor) plus sintilimab (an anti-programmed death 1 antibody; F&S) in previously treated patients with MSS-mCRC and to explore predictive biomarkers.Methods In this prospective observational study, patients with mCRC receiving F&S as third-line or subsequent treatment were enrolled. …”
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Evaluasi penggunaan tepung cangkang rajungan sebagai bahan baku pakan juwana udang windu Penaeus monodon by Agus Kurnia, Wellem H Muskita, Oce Astuti, , Asnani, Wulandari Harahap

“…ABSTRACT   Feeding trial was conducted to determine the optimum dietary level of the crab shell meal (CSM) for replacement of fish meal (FM) for growth and survival rate of black tiger shrimp larvae. …”
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Multilocation Test of Prospective Composite Sticky Corn Varieties With High Production in Indonesia by null Edy, Andi Takdir Makkulawu, null Aminah, St Subaedah

“…Sticky corn is required to meet the demand for a national staple food, driven by population growth. In this context, different efforts are carried out to increase productivity by using new superior varieties with easy adaptation to specific and wider environments as well as high resistance to pests and diseases. …”
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The prevalence of anemia and the factors associated with its severity among children aged 6-59 months in Ghana: A multi-level ordinal logistic regression. by Yordanos Sisay Asgedom, Aklilu Habte, Beshada Zerfu Woldegeorgis, Mengistu Meske Koyira, Beimnet Desalegn Kedida, Bezawit Melak Fente, Amanuel Yosef Gebrekidan, Gizachew Ambaw Kassie

“…It can result in severe consequences, such as stunted growth, impaired cognitive and motor development, increased illness, and even death. …”
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Atezolizumab, bevacizumab, pemetrexed and platinum for EGFR‐mutant NSCLC patients after EGFR TKI failure: A phase II study with immune cell profile analysis by Shang‐Gin Wu, Chao‐Chi Ho, James Chih‐Hsin Yang, Shu‐Han Yu, Yen‐Feng Lin, Shu‐Chin Lin, Bin‐Chi Liao, Ching‐Yao Yang, Yen‐Ting Lin, Chong‐Jen Yu, Ya‐Ting Chuang, Wei‐Yu Liao, Kah Yi Yap, Weng Si Kou, Jin‐Yuan Shih

“…Abstract Background Acquired resistance to epidermal growth factor receptor tyrosine kinase inhibitors (EGFR TKIs) remains a significant hurdle for patients with EGFR‐mutated non‐small cell lung cancer (NSCLC), particularly those lacking the EGFRT790M. …”
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Hybrid azole-based conjugates as upcoming anticancer and antimicrobial agents by Luís M. T. Frija, Bruno E. C. Guerreiro, Inês C. C. Costa, Vera M. S. Isca, Lucília Saraiva, Beatriz G. Neves, Mariana Magalhães, Célia Cabral, Maria L. S. Cristiano, Patrícia Rijo

“…Conclusions: This investigation reveals the potential of distinct azole-based conjugates, in particular the tetrazole-thiadiazolyl (11) derivative, as scaffolds worth further investigations, in the frame of antimicrobial and antineoplastic chemotherapy.…”
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miR-9-5p/HMMR regulates the tumorigenesis and progression of clear cell renal cell carcinoma through EMT and JAK1/STAT1 signaling pathway by Xinyang Niu, Dingheng Lu, Weitao Zhan, Jiazhu Sun, Yuxiao Li, Yuchen Shi, Kai Yu, Suyuelin Huang, Xueyou Ma, Xiaoyan Liu, Ben Liu

“…Specifically, HMMR could be targeted and inhibited by miR-9-5p and might modulate the tumorigenesis and progression of ccRCC through both EMT and JAK1/STAT1 signaling pathway.…”
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Deep Learning and Radiomics in Triple-Negative Breast Cancer: Predicting Long-Term Prognosis and Clinical Outcomes by Cheng C, Wang Y, Zhao J, Wu D, Li H, Zhao H

“…This article reviews recent progress in the application of deep learning, ultrasound, MRI, and radiomics for TNBC diagnosis and treatment, based on research from both domestic and international scholars.Keywords: deep learning, MRI, radiomics, triple negative breast cancer, ultrasound…”
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Clinicopathological Analysis of 14 Cases of Primary Pulmonary Lymphoepithelial Carcinoma by Yixuan FANG, Anzhe WANG, Lumin SHEN, Xiao YUAN, Yu KONG

“…Pathological examination revealed tumor cells exhibiting syncytial-like growth, accompanied by lymphocytic infiltration and stromal fibrosis, with the Regaud type showing well-defined borders combined with granulomatous inflammation, while the Schmincke type exhibited indistinct tumor margins. …”
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Your period and your pregnancy, a cohort study of pregnant patients investigating the associations between menstruation and birth outcomes in Australia: study protocol by Miranda Davies-Tuck, Billie Bradford, Adrienne Gordon, Beverley Vollenhoven, Kirsten Rebecca Palmer, Kirstin Tindal, Caroline E Gargett, Fiona Cousins, Stacey Ellery

“…The primary outcome is a composite of stillbirth, spontaneous preterm birth, pre-eclampsia or fetal growth restriction. Participants will also be invited to complete an optional fetal movements survey at 28–32 and 36+ weeks’ gestation, and consent for placental collection at the time of birth will be sought.Ethics and dissemination Ethics approval was obtained from Monash Health Human Research Ethics Committee (83559) on 24 April 2024. …”
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Disulfidptosis-related gene signatures as prognostic biomarkers and predictors of immunotherapy response in HNSCC by Haotian Qin, Haotian Qin, Juan Xu, Yaohang Yue, Yaohang Yue, Meiling Chen, Zheng Zhang, Panpan Xu, Yan Zheng, Hui Zeng, Jian Weng, Jian Weng, Jun Yang, Fei Yu

“…DSTN upregulation promotes tumor growth, and its knockout inhibits proliferation, migration, and invasion.…”
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Uji Efek Antibakteri Ekstrak Etanol Kulit Batang Salam {Syzigium polyanthum (Wight) Walp} terhadap Staphylococcus aureus dan Escherichia coli secara Invitro by Ikhsan Amanda Putra, Erly ., Machdawaty Masri

“…The results showed that the ethanol extract of Salam bark has antibacterial effects to Staphylococcus aureus with the concentration of 25%, 50%, 75%, and 100% gives the inhibition of the growth of bacteria on 12 mm, 13.67 mm, 12.33 mm, and the 9 mm, while at the same concentration for Escherichia coli was no bacterial inhibition area. …”
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The characters of fairy tales in the children's book illustration: a dragon and a witch by Ingrida Korsakaitė

“…As a rule, she is bareheaded. The clothes of the witch include some ethnographical elements. …”
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