High clinical utility of long-read sequencing for precise diagnosis of congenital adrenal hyperplasia in 322 probands by Yunpeng Wang, Gaohui Zhu, Danhua Li, Yu Pan, Rong Li, Ting Zhou, Aiping Mao, Libao Chen, Jing Zhu, Min Zhu

“…In the perspective study, 96 probands were identified using both the control method and T-LRS. Concordant results were detected in 85.42% (82/96) of probands. …”
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Replication kinetics, pathogenicity and virus-induced cellular responses of cattle-origin influenza A(H5N1) isolates from Texas, United States by Ahmed Mostafa, Ramya S. Barre, Anna Allué-Guardia, Ruby A. Escobedo, Vinay Shivanna, Hussin Rothan, Esteban M. Castro, Yao Ma, Anastasija Cupic, Nathaniel Jackson, Mahmoud Bayoumi, Jordi B. Torrelles, Chengjin Ye, Adolfo García-Sastre, Luis Martinez-Sobrido

“…Unlike rHPbTX and rLPbTX, both rHPhTX and rLPhTX exhibited higher pathogenicity and efficient replication in infected C57BL/6J mice. …”
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Circulating Th1 and Th2 Subset Accumulation Kinetics in Septic Patients with Distinct Infection Sites: Pulmonary versus Nonpulmonary by Ming Xue, Yuying Tang, Xu Liu, Mingyuan Gu, Jianfeng Xie, Ling Liu, Yingzi Huang, Fengmei Guo, Yi Yang, Haibo Qiu

“…Compared to 28-day survivors, higher Th2/Th1 driven by increased Th2 were observed among 28-day nonsurvivors on D3 and D7 in both groups. The association between circulatory Th2 populations or Th2/Th1 and 28-day death was detected in pulmonary sepsis (P<0.05, HR>1), rather than nonpulmonary sepsis. …”
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Alleviating Tumor Hypoxia and Immunosuppression via Sononeoperfusion: A New Ally for potentiating anti-PD-L1 blockade of solid Tumor by Guoliang Yang, Hui Li, Jiabei Yin, Lei Yao, Jun Yang, Jiawei Tang, You Wu, Meng Zhou, TingTing Luo, Yi Zhang, Jing Zhang, Xuezhi Yang, XiaoXiao Dong, Zheng Liu, Ningshan Li

“…Furthermore, the improved immune TME by sononeoperfusion effect sensitized anti-PD-L1 treatment both in MC38 colon cancer and Lewis lung carcinoma mice, resulting in tumor regression and prolonged survival. …”
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Deficiency of FABP7 Triggers Premature Neural Differentiation in Idiopathic Normocephalic Autism Organoids by Xiao Han, Yuanlin He, Yuanhao Wang, Wenzhu Hu, Chu Chu, Lei Huang, Yuan Hong, Lu Han, Xu Zhang, Yao Gao, Yuan Lin, Hongxia Ma, Hongbing Shen, Xiaoyan Ke, Yan Liu, Zhibin Hu

“…It is subsequently revealed alterations in the phosphorylation levels of Mitogen‐Activated Protein Kinase Kinase 1/2 (MEK1/2), which are downstream of FABP7, and the regulation of the FABP7/MEK pathway reversed improper neural differentiation in the ASD organoids. Moreover, both Fabp7‐knockdown and MEK2‐overexpressing mice exhibited repetitive stereotyped behaviors and social defects relevant to autism. …”
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HistoNeXt: dual-mechanism feature pyramid network for cell nuclear segmentation and classification by Junxiao Chen, Ruixue Wang, Wei Dong, Hua He, Shiyong Wang

“…Conclusion By integrating novel convolutional technology and employing a pyramid fusion of dual-mechanism characteristics, HistoNeXt enhances both the precision and efficiency of nuclear segmentation and classification. …”
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Anti-PL-7/PL-12 antisynthetase syndrome associated with interstitial lung disease following SARS-COV-2 infection and vaccination: A case study review by Laura García-Bravo, Ángela Villegas, Bárbara López Uceda, Anaís Mariscal, Cristina Vadillo, M Asunción Nieto Barbero, Juan Luis Rodríguez-Hermosa, Beatriz Mediero Valeros, José Carlos Plaza-Hernández, Miguel Fernández-Arquero, María Guzmán-Fulgencio, Gloria Candelas-Rodríguez, Silvia Sánchez-Ramón, Juliana Ochoa-Grullón

“…Anti-ARS autoantibodies were identified using three complementary methods: immunoblotting, western blotting (WB) and the method considered the gold standard, immunoprecipitation (IP), which ensures accurate interpretation of results. …”
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Genome-Wide Comprehensive Identification and In Silico Characterization of Lectin Receptor-Like Kinase Gene Family in Barley (Hordeum vulgare L.) by Fee Faysal Ahmed, Farah Sumaiya Dola, Md Shohel Ul Islam, Fatema Tuz Zohra, Nasrin Akter, Shaikh Mizanur Rahman, Md. Abdur Rauf Sarkar

“…Exploration of gene ontology (GO) enrichment indicated that the identified HvlecRLK genes are associated with various cellular processes, metabolic pathways, defense mechanisms, kinase activity, catalytic activity, ion binding, and other essential pathways. …”
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Case report: Clinical characteristics and Genetical analysis of HSD11B2 in three Chinese children with apparent mineralocorticoid excess: a case series by Yuan Ding, Yuan Ding, Ming Cheng, Ming Cheng, Bingyan Cao, Bingyan Cao, Min Liu, Min Liu, Xuyun Hu, Xuyun Hu, Di Wu, Di Wu, Di Wu

“…Treatment with oral spironolactone and potassium chloride resulted in the normalization of both blood pressure and serum potassium levels in all patients.ConclusionThis study presents the diagnostic and treatment experiences of three Chinese pediatric patients with AME type I. …”
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Autosomal Recessive Bestrophinopathy: Clinical and Genetic Characteristics of Twenty-Four Cases by Hassan Khojasteh, Mohsen Azarmina, Nazanin Ebrahimiadib, Narsis Daftarian, Hamid Riazi-Esfahani, Houra Naraghi, Hamideh Sabbaghi, Alireza Khodabande, Hooshang Faghihi, Afrooz Moghaddasi, Fatemeh Bazvand, Masoud Reza Manaviat, Hamid Ahmadieh, Narges Hassanpoor, Fatemeh Suri

“…Eighteen patients (75%) had a thick choroid on EDI-OCT, of which three had advanced angle-closure glaucoma, 15 patients were hyperopic, and eight of them had more than four diopters hyperopia in both eyes. Macular retinoschisis was observed in 46 eyes of 23 patients (95%) with cysts mostly located in the inner nuclear layer (INL) to the outer nuclear layer (ONL). …”
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Host factor PLAC8 is required for pancreas infection by SARS-CoV-2 by Lesly Ibargüen-González, Sandra Heller, Darío López-García, Hanna Dietenberger, Thomas FE Barth, Patricia Gallego, Israel Fernández-Cadenas, Sayoa Alzate-Piñol, Catalina Crespí, Julieth A. Mena-Guerrero, Eugenia Cisneros-Barroso, Alejandro P. Ugalde, Gabriel Bretones, Charlotte Steenblock, Alexander Kleger, Marta L. DeDiego, Carles Barceló

“…Interestingly, we find an association between PLAC8 expression and SARS-CoV-2 infection in postmortem analysis of COVID-19 patients both in the pancreas and in other bonafide SARS-CoV-2 target tissues. …”
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External negative pressure improves lung aeration in near-term rabbit kittens at risk of developing respiratory distress by C. Diedericks, C. Diedericks, K. J. Crossley, K. J. Crossley, D. Jurkschat, M. J. Wallace, M. J. Wallace, I. M. Davies, I. M. Davies, P. J. Riddington, P. J. Riddington, A. B. te Pas, M. J. Kitchen, M. J. Kitchen, S. B. Hooper, S. B. Hooper

“…Kittens were ventilated with an 8 ml/kg tidal volume and PEEP of 0 cmH2O and lungs imaged using phase contrast x-ray imaging.ResultsCompared to external atmospheric pressures, external negative pressures expanded the chest (by 2100 ± 43 vs. 1805 ± 59 mm2; Control kittens; P = 0.028), directed tidal ventilation into lower, larger lung regions and increased functional residual capacity (FRC) levels in both Control (26.7 ± 2.0 vs. 12.6 ± 2.2 ml/kg; P &lt; 0.001) and EL (19.6 ± 1.6 vs. 10.0 ± 2.9 ml/kg; P &lt; 0.01) kittens. …”
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The effects of TECAR therapy on pain, range of motion, strength and subscale of HAGOS questionnaire in athletes with chronic adductor related groin pain: a randomized controlled tr... by Sara Nazari, Soheil Mansour Sohani, Javad Sarrafzadeh, Hooman Angoorani, Abbas Tabatabaei

“…A total of 22 male professional athletes (mean age 21.36 years) were randomly assigned to either the real TECAR therapy (n = 11) or sham TECAR therapy (n = 11) group, using block-balanced randomization. Both groups received stretching exercises. Intervention group received 10 sessions of TECAR therapy while, the control group received sham TECAR therapy. …”
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Anemia during pregnancy and adverse pregnancy outcomes: a systematic review and meta-analysis of cohort studies by Rui Wang, Shan Xu, Xiaoyu Hao, Xingyi Jin, Da Pan, Hui Xia, Wang Liao, Ligang Yang, Shaokang Wang, Shaokang Wang

“…This study aims to explore the relationship between anemia and adverse pregnancy outcomes such as preterm birth, low birth weight, and maternal mortality.MethodsWe conducted both a systematic review and a meta-analysis on the associations between anemia during pregnancy and adverse pregnancy outcomes. …”
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Efficacy and safety of acupuncture monotherapy or combined with pelvic floor muscle training for female stress urinary incontinence: a systematic review and meta-analysis by Tong Jiang, Zhi-Yu Dong, Ying Shi, Yang-Qing Zhou, Hong-Bin Zhang, Yi Gong

“…Acupuncture’s role in improving SUI is also unclear based on previous studies.ObjectivesThis systematic review and meta-analysis evaluated the efficacy and safety of acupuncture individually or combined with PFMT in improving symptoms and women’s health-related quality of life of SUI.DesignA systematic review and meta-analysis were conducted.PatientsWomen with stress urinary incontinence were treated with electroacupuncture (EA) individually or acupuncture combined with PFMT.ResultsIn the analysis comparing urinary leakage 1 h and the ICIQ-SF score, electroacupuncture (EA) intervention was significantly associated with improvements in both urine leakage (MD = 4.69, 95% CI 2.83 to 6.55, I2 = 62%) and ICIQ-SF (MD = 2.61, 95% CI 1.64 to 3.58, I2 = 65%). …”
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Impact of hepatic steatosis on the efficacy of antiviral treatment for chronic hepatitis B and the establishment of predictive model: a cohort study by Guanghui Ren, Kaining Jia, Shi Yin, Yunpeng Guan, Qingwei Cong, Ying Zhu

“…The pgRNA response was also higher in the CHB + MASLD group at both time points (24 weeks: 30.9% vs. 19.7%, p = 0.163; 48 weeks: 48.8% vs. 28.3%, p = 0.049). …”
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Blood proteomics and multimodal risk profiling of human volunteers after incision injury: A translational study for advancing personalized pain management after surgery by Daniel Segelcke, Julia R. Sondermann, Christin Kappert, Bruno Pradier, Dennis Görlich, Manfred Fobker, Jan Vollert, Peter K. Zahn, Manuela Schmidt, Esther M. Pogatzki-Zahn

“…This pipeline opens new avenues for clinical application with the goal of stratifying patients and identifying potential new targets, as well as mechanistic correlates, for postsurgical pain.…”
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Spatial distribution and determinants of children ever born among reproductive age women in Ethiopia: spatial and multilevel analysis of 2019 mini Ethiopian demographic health surv... by Ahmed Fentaw Ahmed, Bezawit Adane, Tilahun Degu Tsega, Mekides Nigusu, Kalaab Esubalew Sharew, Abebaw Molla, Mulugeta Tesfa

“…From community level variables: a community with high proportion of contraceptive non user [AOR = 1.38, 95% CI (1.94–2.04)] were positively associated with high number of CEB.ConclusionBoth individual and community-level factors were significantly linked to a high number of children born. …”
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Evaluating the Cellophane Production Supply Chain Using Multi-Criteria Decision-Making Approaches by َAhmad Jafarnejad, Amir Mohammad Khani

“…These are very important processes as they could affect the effectiveness and efficiency of the entire chain. Both require careful management and continuous improvement to guarantee the smooth operation of the chain. …”
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Clinical and radiological evaluation of the Bristow–Latarjet procedure in patients with 30 or more years of follow-up by Arnaldo Amado Ferreira Neto, MD, PhD, Mauro Emilio Conforto Gracitelli, MD, PhD, Jorge Henrique Assunção, MD, PhD, Fernando Brandão de Andrade e Silva, MD, PhD, Verônica Yulin Prieto Chang, MD, Eduardo Angeli Malavolta, MD, PhD

“…Screw and graft positioning were assessed by computed tomography, the degree of arthropathy by radiography, and subscapularis fatty degeneration as rotator cuff tears by magnetic resonance imaging. Both clinical scores and imaging were obtained at 30 years of surgery. …”
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