Chimeric cytokine receptor TGF-β RⅡ/IL-21R improves CAR-NK cell function by reversing the immunosuppressive tumor microenvironment of gastric cancer by Yaojun Ren, Min Xue, Xinhui Hui, Xiuyu Liu, Muhammad Asad Farooq, Yiran Chen, Yuzhou Ji, Yixin Duan, Iqra Ajmal, Jie Yao, Wenzheng Jiang

“…We successfully constructed NKG2D-CAR-NK cells expressing TRII/21 R and demonstrated strong anti-tumor activity against cancer cells both in vitro and in vivo. The co-expression of TRII/21 R in CAR-NK cells enhanced the cytotoxicity, promoted proliferation and survival capabilities, and reduced the expression of exhaustion markers. …”
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CYP3A5 promotes glioblastoma stemness and chemoresistance through fine-tuning NAD+/NADH ratio by Wentao Hu, Xiaoteng Cui, Hongyu Liu, Ze Li, Xu Chen, Qixue Wang, Guolu Zhang, Er Wen, Jinxin Lan, Junyi Chen, Jialin Liu, Chunsheng Kang, Ling Chen

“…Loss-of-function or gain-of-function genetic experiments were performed to assess the impact of CYP3A5 on the self-renewal and chemoresistance of GSCs both in vitro and in vivo. Mechanistic studies were conducted using nontargeted metabolomics, RNA-seq, seahorse, transmission electron microscopy, immunofluorescence, flow cytometry, ChIP‒qPCR, RT‒qPCR, western blotting, etc. …”
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Association between physical activity, smartphone usage, and obesity risk among Korean adolescents: A cross-sectional study based on 2021 Korean adolescent health behavior survey by Haoyuan Li, Jaeyoung Choi, Aram Kim, Guifang Liu

“…Similarly, females exercising “3–4 times/week” (OR = 1.208, 95 % CI: 1.048–1.393, p < 0.01) or “≥ 5 times/week” (OR = 1.333, 95 % CI: 1.087–1.636, p < 0.01) also showed increased risk. Both males and females participating in high-intensity PA (HIPA) “1–2 times/week” were at higher risk (males: OR = 1.110, 95 % CI: 1.011–1.219, p < 0.01; females: OR = 1.427, 95 % CI: 1.283–1.587, p < 0.001). …”
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Knowledge, attitudes, and practices among physicians and pharmacists toward antibiotic use in sepsis by Jingmin Zhang, Jingmin Zhang, Haipeng Shi, Haipeng Shi, Yanmei Xia, Yanmei Xia, Zhenghua Zhu, Yaojun Zhang

“…Nonetheless, the findings also reveal the persistence of certain misconceptions, alongside notable shortcomings in both attitudes and practices. Comprehensive training programs are imperative for enhancing the practices of physicians and pharmacists in this field.…”
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The role of FAM171A2-GRN-NF-κB pathway in TBBPA induced oxidative stress and inflammatory response in mouse-derived hippocampal neuronal HT22 cells by Yi Wang, Jing Shan, Ling Zhang, Rui Wang, Meng-Yu Wu, Hong-Mei Li, Hai-Ming Xu

“…The results showed that TBBPA exposure could lead to an increase in the production of inflammation-related genes IL-6, iNOS, TGF-β1, COX2, and TNF-α in both HT22 cells and HT22-AD-model, intensifying the inflammatory response; it inhibits the mRNA expression of antioxidative enzymes genes Sod1, Cat, Gpx1, and Gsta1, resulting in reduced antioxidant enzyme activities of SOD, CAT, and GSH-Px/GPX. …”
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Single-cell RNA sequencing elucidates cellular plasticity in esophageal small cell carcinoma following chemotherapy treatment by Qinkai Zhang, Qinkai Zhang, Qinkai Zhang, Ziyu Gao, Ru Qiu, Jizhao Cao, Chunxiao Zhang, Wei Qin, Wei Qin, Meiling Yang, Meiling Yang, Xinyue Wang, Ciqiu Yang, Jie Li, Jie Li, Dongyang Yang, Dongyang Yang

“…In this study, weemployed single-cell RNA sequencing (scRNA-seq) to analyze SCCE samplesfrom a single patient both before and after chemotherapy treatment. Our analysisrevealed significant cellular plasticity and alterations in the tumormicroenvironment’s cellular composition. …”
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Tangeretin alleviates sepsis-induced acute lung injury by inhibiting ferroptosis of macrophage via Nrf2 signaling pathway by Hui Zhang, Yan Wang, Shenghua Wang, Xiaomei Xue, Kai Huang, Dunfeng Xu, Lai Jiang, Siyuan Li, Yunqian Zhang

“…Glutathione (GSH), malondialdehyde (MDA) levels, reactive oxygen species (ROS) and inflammatory factors were also determined both in vivo and in vitro. Furthermore, mice were treated with an Nrf2 inhibitor (ML385) to verify the mechanism of tangeretin in inhibiting sepsis-induced lung injury and ferroptosis. …”
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Reticulocyte hemoglobin content: a new frontier in iron deficiency diagnostics for major surgical patients by Suma Choorapoikayil, Mischa J. Kotlyar, Lisa Kawohl, Paul P. Pratz, Denana Mehic, Peter Kranke, Florian Rumpf, Lea V. Blum, Jan A. Kloka, Kai Zacharowski, Vanessa Neef, Patrick Meybohm

“…Method In this retrospective study, 2,966 major surgical patients from two University Hospitals were screened for the presence of ID and the significance of Ret-He in diagnosis ID was evaluated in both non-anemic and anemic patients. According to hemoglobin, ferritin, and transferrin saturation concentrations patients were assigned to a Control group (no anemia, no ID), ID group (no anemia, ID), IDA group (anemia, ID) or Others group (anemia, no ID). …”
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Left atrial strain predicts paroxysmal atrial fibrillation recurrence after catheter ablation: a 1-year study using three-dimensional speckle-tracking echocardiography by Rui Zhang, He Li, Yan Wang, Tianle Yu, Jiacheng Li, Yumeng Wu, Zhiwen Yu, Cuixing Liang, Dan Yu, Li Xue

“…Notably, patients who sustained SR demonstrated significant improvements in various LA strain parameters, including reservoir, pump, and conduit functions, compared to both their preoperative levels and those of patients who experienced recurrence(p < 0.05). …”
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Advancements in noninvasive techniques for transplant rejection: from biomarker detection to molecular imaging by Yuan Song, Yihui Wang, Wenyuan Wang, Yuji Xie, Junmin Zhang, Jing Liu, Qiaofeng Jin, Wenqian Wu, He Li, Jing Wang, Li Zhang, Yali Yang, Tang Gao, Mingxing Xie

“…Biopsy, although considered the gold standard, is invasive, costly, and unsuitable for routine monitoring. …”
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The Role of OCT Angiography in the Assessment of Epiretinal Macular Membrane by Daniela Bacherini, Francesco Dragotto, Tomaso Caporossi, Chiara Lenzetti, Lucia Finocchio, Alfonso Savastano, Maria Cristina Savastano, Francesco Barca, Martina Dragotto, Lorenzo Vannozzi, Francesco Nasini, Francesco Faraldi, Stanislao Rizzo, Gianni Virgili, Fabrizio Giansanti

“…We observed a large increase in both the perfusion density (PD) and the VD of the deep capillary plexus between baseline and the 6-month follow-up (p < 0.001). …”
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A novel nomogram based on the patient’s clinical data and CT signs to predict poor outcomes in AIS patients by Jingyao Yang, Fangfang Deng, Qian Zhang, Zhuyin Zhang, Qinghua Luo, Yeyu Xiao

“…Results from the Hosmer-Lemeshow goodness-of-fit test and calibration plot indicated consistent performance of the prediction model across both training and validation cohorts. …”
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MAF1 inhibits hepatocarcinogenesis by fostering an immunostimulatory tumor microenvironment by Di Cao, Shi-Juan Mai, Hui-yun Wang, Ge Ren, Yue-Ning Wang, Chao-Yue Sun, Haojiang Li, Yu-Feng Zhou, Mei-Yin Zhang, Shuo-Cheng Wang

“…Cell lines were analyzed using RNA sequencing, immunoblotting, dual luciferase reporter, and chromatin precipitation assays.Results Both MAF1-WT and MAF1-4A proteins significantly inhibit hepatocarcinogenesis in mice, with the mutant form exhibiting a stronger suppressive effect. …”
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Protective effects and possible mechanisms of mesenchymal stem cells and mesenchymal stem cell-derived extracellular vesicles against kidney fibrosis in animal models: a systematic... by Yuanchen Lv, Zibo Hua, Xiaomei Lu

“…The term “CD68+ macrophages” refers to macrophages that express the CD68 protein on their cell surface. Both macrophages and myofibroblasts have been linked to the development of kidney fibrosis. …”
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PSTR: A Test Case Reuse Method Based on Path Similarity by Xinyue Xu, Sinong Chen, Zhonghao Guo, Xiangxian Chen

“…First, static analysis is performed on both the old and new code versions to generate their respective sets of static paths. …”
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Task-shifted approaches to postdiagnostic dementia support: a qualitative study exploring professional views and experiences by Louise Robinson, Greta Rait, Claire Bamford, Jill Manthorpe, Rachael Frost, Jane Wilcock, Karen Harrison Dening, Martin Knapp, Sube Banerjee, Joy Adamson, Alistair Burns, Raphael Wittenberg, Kate Walters, Alison Wheatley, Greta Brunskill, Louise Allan, Lynne Corner, Donna Buxton, Katherine Jacques, Doug Lewins, Sue Tucker

“…Potential benefits of a task-shifted approach were continuity and a more holistic approach to care; familiarity for both patients and staff; and reduction of stigma. …”
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Tracheostomy Practice in the Italian Intensive Care Units: A Point-Prevalence Survey by Raffaele Merola, Maria Vargas, Filippo Sanfilippo, Marco Vergano, Giovanni Mistraletti, Luigi Vetrugno, Gennaro De Pascale, Elena Giovanna Bignami, Giuseppe Servillo, Denise Battaglini

“…Tracheostomy procedures were typically performed after a mean duration of 12.9 days of mechanical ventilation, primarily due to difficulties related to prolonged weaning (64.5%). Both early and late complications were observed, and 19.35% of tracheostomized patients did not survive beyond one month following the procedure. …”
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Efficacy and safety of first-line or second-line selective laser trabeculoplasty for normal-tension glaucoma: a multicentre cohort study by Tomoko Naito, Kazuyuki Hirooka, Toru Nakazawa, Masaki Tanito, Shiro Mizoue, Katsuyoshi Suzuki, Keiji Yoshikawa, Itaru Kimura, Yasushi Ikuno, Takako Miki, Kazuhisa Sugiyama, Tairo Kimura, Akiko Narita, Tadashi Nakano, Tomoyuki Watanabe, Koji Nitta, Kae Sugihara, Maki Katai, Takuji Matsuda, Hiroaki Ozaki, Kaori Komatsu, Yoshiaki Saito, Takeshi Sagara, Aika Tsutsui, Akiko Ishida, Satoru Tsuda, Toyoaki Tsumura, Naoki Tojo, Naoto Tokuda, Kenji Nakamoto, Naka Shiratori, Mami Nanno, Naoya Nezu, Yoshitaka Tasaka, Shigeru Mori, Shigeki Yamabayashi, Kimihito Konno, Miyuki Domoto

“…The mean IOP of 16.3±2.1 mm Hg before SLT decreased by 17.1%±9.5% to 13.4±1.9 mm Hg at 12 months after SLT in the first-line group (p&lt;0.001), and the mean IOP of 15.4±1.5 mm Hg before SLT decreased by 12.7%±9.7% to 13.2±2.0 mm Hg at 12 months after SLT (p=0.005) in the second-line group. Both groups showed significant reductions in IOP. …”
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CHARACTERISTICS OF CYTOPROTECTIVE EFFECT ON THE GASTRIC MUCOSA OF CRYOPRESERVED PLACENTA EXTRACT UNDER WATER-IMMOBILIZATION STRESS by Illia Koshurba, Fedir Hladkykh, Mykola Chyzh

“…According to the degree of modulation of the activity of both total NOS and its individual isoforms, the study showed that preventive five-day administration of esomeprazole is inferior to the effectiveness of CEP. …”
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Preoperative mapping techniques for brain tumor surgery: a systematic review by Augusto Leone, Augusto Leone, Francesco Carbone, Francesco Carbone, Uwe Spetzger, Peter Vajkoczy, Giovanni Raffa, Flavio Angileri, Antonino Germanó, Melina Engelhardt, Thomas Picht, Thomas Picht, Antonio Colamaria, Tizian Rosenstock, Tizian Rosenstock

“…These limitations restrict fMRI’s role in preoperative planning for both motor and language functions. While MEG offers high accuracy in motor mapping, its high cost and technical complexity contribute to the limited number of available studies. …”
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