In vitro functional validation of anti-CD19 chimeric antigen receptor T cells expressing lysine-specific demethylase 1 short hairpin RNA for the treatment of diffuse large B cell l... by Zhi Guo, Zhi Guo, Mingxin He, Ning Liu, Yiqing Yang, Rui Sun, Jianxun Wang, Qiang Wang

“…Epigenetic modifications can regulate T cell activation, maturation and depletion; they can be applied to reduce T cell depletion, improve infiltration, and promote memory phenotype formation to reduce relapse after CAR-T cell therapy.PurposeWe propose to develop and validate in vitro the function of novel CAR-T cells for the treatment of DLBCL, which simultaneously express an anti-CD19 CAR with lysine-specific demethylase 1 (LSD1) short hairpin (sh)RNA to prevent depletion and prolong the survival of CAR-T cells.MethodsWe designed an shRNA sequence targeting LSD1 mRNA, and created a vector with the following elements: the U6 promoter driving expression of the LSD1 shRNA sequence, the EF1a promoter driving a second-generation anti-CD19 CAR sequence encoding an anti-CD19 single-chain variable fragment (FMC63), the CD8 hinge and transmembrane structural domains, the CD28 co-stimulatory structural domain, and the CD3ζ-activating structural domain. …”
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Penerapan Hidden Markov Model (HMM) dan Mel-Frequency Cesptral Coefficients (MFCC) pada E-Learning Bahasa Madura untuk Anak Usia Dini by Ubaidi Ubaidi, Nindian Puspa Dewi

“…Thus, it’s considered important to create Bahasa Madura e-learning by implementing the recognition of voice patterns in order to make it easier for the children to learn Bahasa Madura which has several variations of pronunciation only for one single object. …”
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La poésie lyrique, un outil d’unification linguistique et politique by Anne-Marie Le Baillif

“…He was also required to manage the reception of Polish ambassadors who came to Tuileries and Fontainebleau to fetch the duke of Alençon (the future king Henri III of France) as new elected polish king. The official artist Antoine Caron has left paintings and drawings of these entertainments. …”
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PENERAPAN PROJECT BASED LEARNING MODEL UNTUK MENINGKATKAN SIKAP ILMIAH MAHASISWA DALAM RANGKA MENGHADAPI ERA MERDEKA BELAJAR by Andik Purwanto, Desy Hanisa Putri, Dedy Hamdani

“…Kata  kunci: Project Based Learning Model, merdeka belajar, sikap ilmiah   ABSTRACT   Thermodynamics is a branch of physics that studies natural phenomena or phenomena that occur in everyday life, especially regarding heat and its transfer. Thermodynamics subject learning often creates problems because it is not precise in choosing a learning model, so it is not able to optimize the learning process which in turn will cause the development of students' scientific attitudes to be less than optimal. …”
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Physicians’ Perceptions of the Quality of Palliative Care and of the Importance of Collaboration in Interdisciplinary Teams in Norwegian Nursing Homes by Skomakerstuen Ødbehr L, Hov R, Sanaker H, Sandsdalen T

“…The quality also depended on the interdisciplinary team’s collaboration in assessing the patient, observing symptoms, and planning further care and treatment in accordance with patients’ and their relatives’ preferences and wishes. Finally, systemic and environmental factors affected the ability to ensure continuity of care. …”
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Evaluation of Prayer Windows with Inscriptions Located Around the Mausoleum of Eyup Sultan by Saliha Tanık, Aliye Öten

“…The photographs were taken in detail and shared as general views and only general views were used in order not to exceed the volume of the article. The gravestones related to the subject in the background of the windows were also identified one by one by scanning the whole of the treasuries. …”
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Optimasi Convolutional Neural Network Untuk Deteksi Covid-19 pada X-ray Thorax Berbasis Dropout by I Gede Totok Suryawan, I Putu Agus Eka Darma Udayana

“…Therefore, this study aimed to tackle that problem by creating a leaner network. Thus, it is easier for others to build without having enormous computing power. …”
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Designing a Model for Implementing Digital Banking Policy Based on Using Big Data in Iranian Banking Industry by Rahmatollah Gholipour Souteh, Hamed Esmaeili Rad

“…Proper utilization of big data could create a necessary competitive advantage, enabling banks to survive and thrive amidst competition from both new and old players in the banking sector. …”
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Reproduction performance of climbing perch Anabas testudineus F1 and F2 broodstock with different dietary supplementation by Helmizuryani, Helmizuryani, Boby Muslimin, Khusnul Khotimah

“…This species is potential to create the sustainability production in the future because they offer a promising price, so it needs a further study for optimizing the quality of broodstock and its offsprings. …”
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Fistula Vesiko Vaginalis by Luki Ertandri

“…On Per Speculum examination, clearly see there are fluids on fornix posterior. methylene blue tes has proceed and get the positive result. …”
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The Influence of Depression, Anxiety, and Stress on Ageism Among Undergraduates: Mediating Roles of Life Satisfaction, Gratitude, and Prosociality by Chen S, Wan Z

“…We examined how DAS is related to ageist attitudes and whether positive traits, such as life satisfaction, gratitude, and prosociality (the tendency to help others), could lessen the negative influence of DAS on ageism.Our findings show that students with higher levels of depression, anxiety, or stress are more likely to hold negative views about older adults. …”
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Perancangan User Experience Aplikasi Penghemat Listrik myECO dengan Otomatisasi dan Manajemen Listrik untuk Rumah Tangga by Maulana Derifato Achmad, Herman Tolle, Buce Trias Hanggara

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Teacher performance analysis using analytic network process method by Zuraidah, Nurul Hudha Purnomo

“…With five main criteria as priorities in assessing performance, namely carrying out learning coherently with a weight of 0.1730, conveying learning material clearly with a weight of 0.1510, showing mastery of learning material with a weight of 0.1240, showing an open attitude towards student responses with a weight of 0.1170 and mastering class with a weight of 0.1100…”
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Uncovering the Mechanisms of BaBaoWuDanYaoMo Against Influenza A Virus and Virus-Induced Inflammation Based on Network Pharmacology and Pharmacological Evaluation by Lei B, Su Y, Chen R, Chen Z, Liu B, Chen Y, Zhou M, Wang X, Ma Q

“…It could inhibit PR8-induced apoptosis and inhibit the expression of apoptosis markers (cleaved PARP). BB inhibited the mRNA expression of MCP-1/CCL-2, IL-6, CXCL-8/IL-8, TNF-α and CXCL-10/IP-10, and downregulated the protein expression of phosphorylated AKT/p38 and TLR3 in vitro. …”
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Kuiyangling Enema Alleviates Ulcerative Colitis Mice by Reducing Levels of Intestinal NETs and Promoting HuR/VDR Signaling by Zhang D, Zhu Z, He Z, Duan S, Yi Q, Qiu M, Dai X, Su G, Li K, Xu L, Liu D, Wu Y, Gao Y, Li R, Guo S

“…The aim of this study is to explore the mechanism of Kuiyangling in the treatment of ulcerative colitis.Methods: A mouse model of ulcerative colitis using 3% DSS water was considered, and model, normal, Kuiyangling medium- (5 g·kg− 1) and high-dose (10 g·kg− 1), and mesalazine (50 mg·kg− 1) groups were created. Measurements of colon length, spleen index, histopathological variances, subcellular structure observations, ROS content, and NET-related proteins (PAD4, MPO, citH3) were obtained through HE staining, electron microscopy, live imaging, and Western blotting assays. …”
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Evaluasi TKTI Puri Saron Hotel Seminyak dengan Framework Cobit 5 by Norsa Yudhi Arso, Made Wisnu Antara Kesiman, Dewa Gede Hendra Divayana

“…Evaluation refers to the framework created by mapping the COBIT 5 domain to the results of the current state assessment. …”
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L’africanisation de l’Église évangélique au Maroc : revitalisation d’une institution religieuse et dynamiques d’individualisation by Bernard Coyault

“…This cohabitation of heterogeneous religious identities within the same institutional structure often creates tensions and misunderstandings. Varying theological reference points, patterns of church organization, and religious practices bear witness to this pluralism-in-tension.The EEAM acts as a resonance chamber for a global, multiform African religious field, with each church member reflecting a particular dimension of this field. …”
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Analisis dan Perancangan Aplikasi Chatbot Menggunakan Framework Rasa dan Sistem Informasi Pemeliharaan Aplikasi (Studi Kasus: Chatbot Penerimaan Mahasiswa Baru Politeknik Astra) by Laksmi Anindyati

“…Sistem informasi penunjang chatbot ini dibangun untuk mengakomodir kebutuhan proses CRUD (Create, Read, Update dan Delete) dari pertanyaan – respon yang nantinya dipelajari oleh chatbot sebelum dapat berinteraksi seperti manusia. …”
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Problems And Challenges Of Attendance In Medical Students by Muhammad Khurram

“…Additional activities include getting clinical electives, and attachments in other national international institutes. …”
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Phenotypes of primary sclerosing cholangitis in children by M.B. Dyba, V.S. Berezenko

“…Primary sclerosing cholangitis (PSC) in children is a rare chronic immune-mediated disease of the biliary tract, which, unlike in adults, has a less aggressive course with da­mage to the intrahepatic bile ducts and is combined with autoimmune hepatitis, creating a special clinical phenotype of PSC, autoimmune sclerosing cholangitis (ASC). …”
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