The necroptosis-related lncRNA ENSG00000253385.1 promotes the progression of esophageal squamous cell carcinoma by targeting the miR-16-2-3p/VDAC1 axis by Xiaoyang Duan, Jian Shi, Ran Hou, Yajie Huang, Chunyan Wang, Huazhen Du

“…Cell proliferation, migration and apoptosis were evaluated by CCK-8 assay, wound healing, transwell cell migration, invasion and flow cytometry assay. The levels of necroptosis-related protein were detected by western blot. …”
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Protective Effects of Baicalein on Lipopolysaccharide-Induced AR42J PACs through Attenuation of Both Inflammation and Pyroptosis via Downregulation of miR-224-5p/PARP1 by Ming-Wei Liu, Chun-Hai Zhang, Shou-Hong Ma, De-Qiong Zhang, Li-Qiong Jiang, Yang Tan

“…An in vitro cell model of acute pancreatitis (AP) was established using lipopolysaccharide (LPS) (1 mg/L)-induced PACs (AR42J), and the relative survival rate was determined using the 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide (MTT) technique. Flow cytometry was applied to evaluate the apoptotic rates of AR42J PACs. …”
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De Novo Acute Myeloid Leukemia with Combined CBFB-MYH11 and BCR-ABL1 Gene Rearrangements: A Case Report and Review of Literature by Venkata Rakesh Sethapati, Ra’ed Jabr, Leyla Shune, Wissam El Atrouni, Patrick R. Gonzales, Wei Cui, Shivani Golem

“…Bone marrow evaluation and flow cytometry analysis were compatible with AML with monocytic features. …”
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Identification and validation of BATF as a prognostic biomarker and regulator of immune cell infiltration in acute myeloid leukemia by Zhe Zhao, Dongmei Wang, Dongmei Wang, Xue Sheng, Shuying Li, Tingting Liu, Mengyuan Chang, Lei Feng, Di Zhang, Chunyan Ji, Chunyan Ji, Fei Lu, Jingjing Ye

“…Meanwhile, we detected the connection between BATF expression and the proportions of immune cells via flow cytometry in C1498 mouse model of AML. Finally, we investigated the association between BATF expression and cell-cell communication within the AML cell population using single-cell sequencing.ResultsIn this study, we thoroughly investigated the role of BATF in AML. …”
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Chemical Composition Analysis of the Patented Naoxueshuan Formula Based on UPLC-Q-TOF MS and GC-MS by Chang LIU, Hui-qin WU, Jiang-bo QI, Hong-ling LIANG, Zhi-hua LIU, Hua-qiang HUANG, Su-ming YANG, Xi ZHOU, Fang HUANG, Hui-tai LUO

“…The mobile phase was comprised of 0.1% formic acid solution and acetonitrile with the flow rate of 0.3 mL/min. MS data was collected under positive and negative ion modes. …”
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lncRNA NEAT1 Downregulation Ameliorates the Myocardial Infarction of Mice by Regulating the miR-582-5p/F2RL2 Axis by Zhenhua Wu, Yunpeng Bai, Yujuan Qi, Chao Chang, Yan Jiao, Yaobang Bai, Zhigang Guo

“…The cell viability, proliferation, and apoptosis were determined by CCK-8, EdU staining, and flow cytometry assays. The expressions of F2RL2, miR-582-5p, and nuclear paraspeckle assembly transcript 1 (NEAT1) in myocardial tissues and HCMs were quantified by qRT-PCR or Western blot. …”
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Systemic hypotension and low perfusion pressure as risk factors for primary open-angle glaucoma progression by O.S. Myakonkaya, A.S. Sarkisyan, A.V. Seleznev, A.V. Kuroyedov, A.Yu. Brezhnev

“…Multiple published data have shown a decrease in blood flow velocity in the retrobulbar vessels, choroid, retina, and optic nerve in patients with glaucoma. …”
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Therapeutic depletion of CCR8+ tumor-infiltrating regulatory T cells elicits antitumor immunity and synergizes with anti-PD-1 therapy by James Dooley, Bart Neyns, Elizabeth Allen, Frank Tacke, Louis Boon, Damya Laoui, Julia Katharina Schwarze, Helena Van Damme, Bruno Dombrecht, Máté Kiss, Heleen Roose, Eva Van Overmeire, Daliya Kancheva, Liesbet Martens, Aleksandar Murgaski, Pauline Madeleine Rachel Bardet, Gillian Blancke, Maude Jans, Evangelia Bolli, Maria Solange Martins, Yvon Elkrim, Kiavash Movahedi, Niels Vandamme, Sebahat Ocak, Isabelle Scheyltjens, Lars Vereecke, Frank Aboubakar Nana, Pascal Merchiers, Jo Agnes Van Ginderachter

“…These findings were validated by flow cytometric analysis of both mouse (LLC-OVA, MC38 and B16-OVA) and human (NSCLC and melanoma) tumor samples. …”
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Oxygenation targets for endovascular therapy in acute ischemic stroke patients (Oxy-TARGET): protocol for a single-centre, open-label randomised controlled trial by Anxin Wang, Zhengfang Hu, Ruquan Han, Fa Liang, Youxuan Wu, Minyu Jian, Xinyan Wang, Yunzhen Wang, Haiyang Liu, Xuan Hou, Kangda Zhang, Zihui Zhang

“…Eligible participants are randomly assigned at a 1:1 ratio to receive FiO2=80% or FiO2=30% through endotracheal intubation, with the gas flow rate set at 4 L/min. The inspiratory oxygen concentration at the tracheal intubation site (delivered FiO2) was recorded concurrently. …”
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Riesgo de contagio por COVID-19 en un entorno urbano (Málaga, noviembre del 2020) by Ángel Miramontes Carballada, Rubén Lois González, María Jesús Perles Roselló, Jesús Miranda Páez

“…Along with the analysis of the spatial distribution of cases and the presence of patterns and trends, aspects such as the analysis of population flows and movements, clinical, social and epidemiological vulnerability, or the socio-economic repercussions of the crisis have been addressed. …”
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Unraveling the immunological landscape and gut microbiome in sepsis: a comprehensive approach to diagnosis and prognosisResearch in context by Yali Luo, Jian Gao, Xinliang Su, Helian Li, Yingcen Li, Wenhao Qi, Xuling Han, Jingxuan Han, Yiran Zhao, Alin Zhang, Yan Zheng, Feng Qian, Hongyu He

“…Methods: Blood samples from 31 septic patients in the Intensive Care Unit (ICU), 25 non-septic ICU patients, and 18 healthy controls were analyzed using flow cytometry for deep immunophenotyping. Metagenomic sequencing was performed in 41 fecal samples, including 13 septic patients, 10 non-septic ICU patients, and 18 healthy controls. …”
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Interleukin-12 Inhibits Tumor Growth and Metastasis Promoted by Tumor-Associated Mesenchymal Stem Cells in Triple-Negative Breast Cancer by Babak Jahangiri, Zahra-Soheila Soheili, Elahe Asadollahi, Mehdi Shamsara, Vahid Shariati, Alireza Zomorodipour

“…Cell proliferation was measured using trypan blue exclusiontechnique, while cell cycle distribution and apoptosis were assessed by flow cytometry. The effects of TA-MSCs ontumor volume, survival rate, and lung metastasis were evaluated by subcutaneous co-injection of MSCs with 4T1 cellsin the right flanks of BALB/c mice (n=5 per group). …”
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Perfusion index as a predictor of successful PNS-guided supraclavicular block: A prospective study by Monika Gandhi, Khushboo Ranpise, Aseem Sharma, Manish Banjare

“…Background: The perfusion index (PI) is a simple, non-invasive measure obtained via pulse oximetry that reflects peripheral blood flow changes. Alterations in PI can signify shifts in perfusion dynamics during a brachial plexus block. …”
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Genomic insights into the alphaproteobacterium Georhizobium sp. MAB10 revealed a pathway of Mn(II) oxidation-coupled anoxygenic photoautotrophy: a novel understanding of the biotic... by Xiuli Xu, Litao Zhang, Fuhang Song, Guoliang Zhang, Linlin Ma, Na Yang

“…This suggested that Mn(II)-derived electrons entered the cyclic photophosphorylation, partially replacing the oxidative phosphorylation for ATP production and enhancing the electron flow to complex I for NADH generation. In vitro enzymatic studies confirmed that GE001273 was a catalyst for Mn(II) oxidation in the outer membrane. …”
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Immunomodulatory Effects of Combination Therapy with Bushen Formula plus Entecavir for Chronic Hepatitis B Patients by Long-Shan Ji, Qiu-Tian Gao, Ruo-Wen Guo, Xin Zhang, Zhen-Hua Zhou, Zhuo Yu, Xiao-Jun Zhu, Ya-Ting Gao, Xue-Hua Sun, Yue-Qiu Gao, Man Li

“…The frequencies of Th1, Treg, and dendritic cells (DCs) and expression levels of PD-1/PD-L1 were examined by flow cytometry. Results. In treatment-naïve CHB patients, complete viral suppression rates in HBeAg(−) patients were higher than those in HBeAg(+) patients in both T and C groups. …”
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Dietary supplementation of Astragalus polysaccharide or its nanoparticles enhances testicular hemodynamics, echotexture, scrotal circumference, concentration of testosterone, estra... by Eman Fayez, Ali Salama, Mohamed Ahmed Ismail, Fady Sayed Youssef, Zaher Mohamed Rawash, MR Oshba, Haney Samir

“…Abstract This study investigated, for the first time, the potential role of Astragalus polysaccharide (APS) and APS nanoparticles on testicular blood flow (TBF) and semen quality in Ossimi rams. Fifteen sexually mature Ossimi rams were allocated randomly into two treated groups that orally administered either nano APS (2 g/ram/day; n = 5) or APS (20 g/ram/day; n = 5) for four weeks and a control group (n = 5). …”
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Migrasomes derived from human umbilical cord mesenchymal stem cells: a new therapeutic agent for ovalbumin-induced asthma in mice by Weifeng Gu, Tingting Zheng, Wen Li, Xinkai Luo, Xiaowei Xu, Ying Wang, Chaoming Mao, Yongbin Ma, Liyang Dong

“…The therapeutic effects of migrasomes on airway inflammation in ovalbumin (OVA)-induced asthmatic mice were evaluated by hematoxylin-eosin (HE) and periodic-acid schiff (PAS) staining, and their mechanism were further testified by immunofluorescent staining, real-time PCR and flow cytometry. Results Here, we showed that inhibition of migrasomes’ production dramatically impaired the anti-inflammatory effects of hUCMSCs in OVA animals, as evidenced by a notable increase in both the infiltration of inflammatory cells and the number of epithelial goblet cells. …”
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High Dimensionality Reduction and Immune Phenotyping of Natural Killer and Invariant Natural Killer Cells in Latent Tuberculosis-Diabetes Comorbidity by Gokul Raj Kathamuthu, Nathella Pavan Kumar, Kadar Moideen, Pradeep A. Menon, Subash Babu

“…Thus, we have studied the unstimulated (UNS), Mycobacterium tuberculosis (Mtb [PPD, WCL]), and mitogen (P/I)-stimulated NK and iNKT cells expressing Type 1 (IFNγ, TNFα, and IL-2), Type 17 (IL-17A, IL-17F, and IL-22) cytokines, cytotoxic (perforin, granzyme B, and granulysin) and immune (GMCSF, PD-1, and CD69) markers in LTB comorbidities by dimensionality reduction and flow cytometry. Our results suggest that LTB DM and PDM individuals express diverse NK and iNKT cell immune clusters compared to LTB NDM individuals. …”
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Prognostic Value of Endothelial Progenitor Cells in Acute Myocardial Infarction Patients by Gongjie Ye, Xiaodan Chen, Yinchao Zhou, Jianqing Zhou, Yongfei Song, Xiaoyong Yang, Lei Yang

“…EPCs, defined as coexpression of CD34+/CD133+ cells or CD133+/CD34+/KDR+, were studied using flow cytometry and categorized by quartiles. Based on the 28-days mortality outcome, the patients were further divided into two groups: death and survival. …”
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Angesinenolide B, A Phthalide Dimeric Peroxide, Exerts Anti-Inflammatory Properties by Suppressing MAPK/STATs Signaling Pathways and ROS Production by Zhang L, Liu Y, Wang H, Guo S, Lv J

“…Fluorescence microscopy and flow cytometry were employed for the determination of ROS generation. …”
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