Evaluation of the diagnostic accuracy of two point-of-care tests for COVID-19 when used in symptomatic patients in community settings in the UK primary care COVID diagnostic accura... by Brian D Nicholson, Philip J Turner, Thomas R Fanshawe, Alice J Williams, Gayatri Amirthalingam, Sharon Tonner, Maria Zambon, Richard Body, Kerrie Davies, Rafael Perera, Simon de Lusignan, Gail N Hayward, F D Richard Hobbs, RAPTOR-C19 Study Group and the CONDOR Steering Committee

“…<h4>Background and objective</h4>Point-of-care lateral flow device antigen testing has been used extensively to identify individuals with active SARS-CoV-2 infection in the community. …”
Get full text

Relationship Between a Heavy Rainfall and Gravity Wave in Sichuan Basin by Xu Yizhou, Li Guoping, Zhang Xiaoyu, Xie Xin, Dong Yuanchang

“…The upper gravity wave is initiated by shear instability and non-equilibrium flow mechanisms at the downslope of the southern Basin, propagating northeast. …”
Get full text

Multimodal magnetic resonance imaging studies on non-motor symptoms of Parkinson's disease by Weimin Qi, Xiaoyan Niu, Xiuping Zhan, Yazhou Ren, Jianhang He, Jianxia Li, Xiaolin Hou, Haining Li

“…We extracted and analyzed differences in regional cerebral blood flow (rCBF), magnetic susceptibility, and gray matter density parameters between the two groups. …”
Get full text

Differential impact of TIM-3 ligands on NK cell function by Xin Zhang, Lazar Vujanovic, Riyue Bao, Robert L Ferris, Tullia C Bruno, Aditi Kulkarni, Hridesh Banerjee, Housaiyin Li, Juncheng Wang, Lawrence P Kane, Pragati Upadhyay, Jennifer L Anderson, Onyedikachi Victor Onyekachi, Lidia M R B Arantes

“…The primary objectives of this study were to assess the prevalence and function of TIM-3+ natural killer (NK) cells in patients with head and neck squamous cell carcinoma (HNSCC), determine whether the four TIM-3 ligands differentially affect TIM-3+ NK cell functions, identify the most immunosuppressive ligand, and evaluate whether targeting ligand-mediated TIM-3 signaling enhances NK cell effector functions.Methods Single-cell RNA sequencing and flow cytometry were used to study the prevalence, phenotypes and function of TIM-3+ NK cells in HNSCC patient tumors and blood. …”
Get full text

Prophylactic ECMO Support during Elective Coronary Percutaneous Interventions in High-Risk Patients: A Single-Center Experience by Claudiu Ungureanu, Marc Blaimont, Hugues Trine, Pierre Henin, Romain Courcelle, Yves Laurent, Patrick Van Ruyssevelt, Caroline Lepièce, Vincent Huberlant

“…The two main triggers in favor of prophylactic VA-ECMO in our studies were the presence of a recent heart failure episode and the high probability of periprocedural prolonged impairment of the coronary flow through the major epicardial artery.…”
Get full text

Clinical Response Predictive Model for Omalizumab in Moderate-to-Severe Asthma Patients by Ruiqi Qian, Lingyi Yang, Xurui Shen, Cheng Chen, Jianan Huang, Xiuqin Zhang, Cuiping Fu

“…Using the improvement in maximal expiratory flow 25% of the measured value to the predicted value (MEF25%pre) as clinical outcome, the significant variables included weight, duration of asthma, use of oral corticosteroids (OCS), total serum IgE level at the 4th week, and history of rhinitis. …”
Get full text

Chemotherapy-induced cellular senescence promotes stemness of aggressive B-cell non-Hodgkin’s lymphoma via CCR7/ARHGAP18/IKBα signaling activation by Yangyang Wang, Fan Wu, Yang Wan, Huiping Wang, Linhui Hu, Qianshan Tao, Zhimin Zhai, Jiyu Wang, Keke Huang, Anwen Ren, Jinlan Li, Liuying Yi, Yanjie Ruan, Zhixiang Wanyan, Chaohong Liu

“…C-C chemokine receptor 7 (CCR7) expression in patients with aggressive B-NHL was assessed using immunohistochemistry and flow cytometry. Lentiviral transfection was used to target CCR7 expression in Raji and SU-DHL-2 cells. …”
Get full text

A scoping review on the obstacles faced by beta thalassemia major patients in Pakistan- Matter of policy investment by Ali Hussain Ansari, Saqib Hussain Ansari, Mubarak Jabeen Salman, Muhammad Usman Hussain Ansari, Rawshan Jabeen

“…For this review, 26 unique articles were identified by using the PRISMA flow guidelines. PubMed and Google Scholar were used with the MESH term Beta-Thalassemia Major AND Pakistan, and the duration was set between 2012–2022. …”
Get full text

Progression of the faecal microbiome in preweaning dairy calves that develop cryptosporidiosis by M. F. Hares, B. E. Griffiths, L. Barningham, E. E. Vamos, R. Gregory, J. S. Duncan, G. Oikonomou, C. J. Stewart, J. L. Coombes

“…Thereafter, sampled calves were monitored for diarrhoeal disease and those that suffered a diarrhoea event were tested for C. parvum by lateral flow testing (LFT). Calves that experienced diarrhoea and tested positive for C. parvum by LFT were assigned to the Cryptosporidium-positive (Cp+) group (n = 32). …”
Get full text

Trichinella spiralis excretory/secretory proteins mediated larval invasion via inducing gut epithelial apoptosis and barrier disruption. by Qi Qi Lu, Wen Wen Zheng, Zhao Yu Zhang, Pei Kun Cong, Xin Guo, Yao Zhang, Xin Zhuo Zhang, Shao Rong Long, Ruo Dan Liu, Zhong Quan Wang, Jing Cui

“…The IIL ESP-induced Caco-2 cell apoptosis was observed by DAPI, Hoechst 33358, TUNEL and Annexin V/PI staining. Besides, flow cytometry revealed an increasing apoptosis rate in Caco-2 cells after the IIL ESP treatment. qPCR and Western blot analysis indicated that IIL ESP activated caspases (Caspase 3, Caspase 9 and Caspase 8), up-regulated the pro-apoptotic factors (Bax and Cytochrome c) and down-regulated the anti-apoptosis molecule Bcl-2. …”
Get full text

A patient-derived HCC spheroid system to model the tumor microenvironment and treatment response by Emilie Crouchet, Nuno Almeida, Sarah C. Durand, Marie Parnot, Marine A. Oudot, Fabio Giannone, Cloé Gadenne, Natascha Roehlen, Antonio Saviano, Emanuele Felli, Patrick Pessaux, Hong Tuan Duong, Hideki Ohdan, Hiroshi Aikata, Kazuaki Chayama, Thomas F. Baumert, Catherine Schuster

“…Characterization of the tumor spheroid cell populations was performed by flow cytometry, immunohistochemistry (IHC), and functional assays. …”
Get full text

Titin gene mutations enhance radiotherapy efficacy via modulation of tumour immune microenvironment in rectum adenocarcinoma by Hengchang Liu, Jialiang Liu, Xu Guan, Zhixun Zhao, Pu Cheng, Haipeng Chen, Zheng Jiang, Xishan Wang

“…ANKRD1 was overexpressed, and clonogenic assays evaluated radiotherapy sensitivity. Flow cytometry, immunofluorescence, and comet assays examined mutations' impact on cell cycle, apoptosis, and DNA damage response (DDR). …”
Get full text

Assessment of vulnerability to fluvial floods: Case of municipalities of the Gidra River Basin, Slovakia by Matej Vojtek, Soheyl Moradi

“…We have calculated a fluvial flood vulnerability index (FFVI) related to the year 2021 for twelve municipalities, which fall into the Gidra River Basin and whose urban area is completely or partially located in the basin, and at the same time a watercourse, belonging to the Gidra River Basin, flows through the municipality. Material and methods: Three indicators (number of buildings within a 100 m radius from the watercourse; length of roads within a 100 m radius from the watercourse; and number of bridges over a watercourse) representing the economic vulnerability, and seven indicators of social vulnerability (population density of municipalities; share of residents aged 65+ in the total number of residents; share of unemployed residents in the total number of economically active residents; share of households with six and more persons in the total number of households; share of incomplete households in the total number of households; share of residents without education and with primary education in the the total number of residents; and share of residents with disabilities in the total number of residents) were used to determine the respective economic flood vulnerability sub-index (EFVsI) and social flood vulnerability sub-index (SFVsI). …”
Get full text

The Performance of Irrigation Schemes in Sudan Affected by Climate Variability and the Grand Ethiopian Renaissance Dam by Shamseddin M. Ahmed, Khalid G. Biro Turk, Hassan Ali Dinar

“…However, the construction of the Grand Ethiopian Renaissance Dam (GERD) in the Blue Nile at the Sudan border has changed water flow regulations along the Blue Nile. Therefore, the Sudanese irrigation schemes that depend on the Blue Nile are affected by the operation and management of the GERD. …”
Get full text

Telenursing on primary family caregivers and children with disabilities: a scoping review by Kaori Nishigaki, Noyuri Yamaji, Noyuri Yamaji, Naho Adachi, Tomoko Kamei, Kyoko Kobayashi, Shota Kakazu, Yuki Yonekura

“…Two or more reviewers individually screened eligible studies according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses flow diagram. Data on the characteristics of the included studies, telecommunications technology interventions, and children with disabilities were mapped in tables.ResultsEleven studies, published between 2003 and 2020, met the inclusion criteria. …”
Get full text

Exosomal mir-126-3p derived from endothelial cells induces ion channel dysfunction by targeting RGS3 signaling in cardiomyocytes: a novel mechanism in Takotsubo cardiomyopathy by Xuehui Fan, Guoqiang Yang, Yinuo Wang, Haojie Shi, Katja Nitschke, Katherine Sattler, Mohammad Abumayyaleh, Lukas Cyganek, Philipp Nuhn, Thomas Worst, Bin Liao, Gergana Dobreva, Daniel Duerschmied, Xiaobo Zhou, Ibrahim El-Battrawy, Ibrahim Akin

“…The immunofluorescence staining, flow cytometry, qPCR, single-cell contraction, intracellular calcium transients, patch-clamp, dual luciferase reporter assay and western blot were performed for the study. …”
Get full text

B Cell Activation, Differentiation, and Their Potential Molecular Mechanisms in Osteoarthritic Synovial Tissue by Lu P, Li Y, Yang S, Yao H, Tu B, Ning R

“…Peizhi Lu,1,2,&ast; Ya Li,1,2,&ast; Shuo Yang,2 Haoyu Yao,2 Bizhi Tu,2 Rende Ning1,2 1Graduate School, Bengbu Medical University, Bengbu, Anhui, People’s Republic of China; 2Department of Orthopedics, the Third Affiliated Hospital of Anhui Medical University, the First People’s Hospital of Hefei, Hefei, Anhui, People’s Republic of China&ast;These authors contributed equally to this workCorrespondence: Bizhi Tu, Department of Orthopedics, the Third Affiliated Hospital of Anhui Medical University, the First People’s Hospital of Hefei, Hefei, Anhui, People’s Republic of China, Tel +86 15357483840, Email 1301535636@qq.com Rende Ning, Graduate School, Bengbu Medical University, Bengbu, Anhui, People’s Republic of China, Tel +86 13956966305, Email nrd1972@outlook.comObjective: The objective of this study was to characterize the activation and differentiation of B cells in the synovium of osteoarthritis (OA) and to explore the underlying molecular mechanisms.Methods: Peripheral blood and synovial samples from OA patients at different stages were collected, and flow cytometry was employed to analyze the activation and differentiation of B cells. …”
Get full text

Novel banana lectin CAR-T cells to target pancreatic tumors and tumor-associated stroma by Malcolm K Brenner, Challice Bonifant, Norihiro Watanabe, Mary K McKenna, Daniel Brenner, Ada Ozcan, Maureen Legendre, Dafydd G Thomas, Christopher Ashwood, Richard D Cummings, David M Markovitz

“…Here, we tested the efficacy of our novel H84T CAR in models of pancreatic ductal adenocarcinoma (PDAC), intractable tumors with aberrant glycosylation and characterized by desmoplastic stroma largely contributed by pancreatic stellate cells (PSCs).Methods We transduced human T cells with a second-generation retroviral construct expressing the H84T BanLec chimeric receptor, measured T-cell expansion, characterized T-cell phenotype, and tested their efficacy against PDAC tumor cells lines by flow cytometry quantification. In three-dimensional (3D) spheroid models, we measured H84T CAR T-cell disruption of PSC architecture, and T-cell infiltration by live imaging. …”
Get full text

Depletion of myeloid-derived suppressor cells sensitizes murine multiple myeloma to PD-1 checkpoint inhibitors by Qiang Wang, Qing Yi, Wei Xiong, Rui Duan, Jianfei Qian, Liuling Xiao, Miao Xian, Pan Su, Chuanchao Zhang, Yabo Li, Ling Zhong, Chengyun Zheng

“…T cell proliferation and function were detected using flow cytometry. Peptide-Fc fusion proteins were used to deplete myeloid-derived suppressor cells (MDSCs). …”
Get full text

Diversity and distribution of nitrifying bacteria play an important role in the nitrogen cycle in mangrove sediments by A. Mubaraq, M. Sembiring, E. Widiastuti, E. Fachrial, B. Utomo, M. Turjaman, F. Sidik, Y.I. Ulumuddin, V.B. Arifanti, E.S. Siregar, T. Kajita, S. Proches, M. Basyuni

“…The Preferred Reporting Items for Systematic Reviews and Meta-Analyses method is used as a guide to help report reviews systematically and has a flow chart to show the process of selecting relevant studies. …”
Get full text