Murine DX5+NKT Cells Display Their Cytotoxic and Proapoptotic Potentials against Colitis-Inducing CD4+CD62Lhigh T Cells through Fas Ligand by Jens M. Werner, Michael Damian, Stefan A. Farkas, Hans J. Schlitt, Edward K. Geissler, Matthias Hornung

“…After polyclonal stimulation with anti-CD3, anti-CD28, and IL-2, proliferation of CD4+CD62Lhigh cells was assessed using a CFSE assay and activity of proapoptotic caspase-3 was determined by intracellular staining and flow cytometry. Extrinsic apoptotic pathway was blocked using an unconjugated antibody against FasL, and activation of caspase-3 was measured. …”
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Optimized Method to Generate Well-Characterized Macrophages from Induced Pluripotent Stem Cells by Qimin Hai, Peter Bazeley, Juying Han, Gregory Brubaker, Jennifer Powers, Claudia M. Diaz-Montero, Jonathan D. Smith

“…Using up to five independent iPSC donors, we performed several assays for macrophage functions and polarization, such as marker protein staining by flow cytometry, lipoprotein uptake, phagocytosis, cytokine release, inflammasome activation, and the effects of M1-like and M2-like polarization. …”
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Identification and validation of the important role of KIF11 in the development and progression of endometrial cancer by Biying Wang, Lunmin Bao, Xiaoduo Li, Guang Sun, Wu Yang, Nanzi Xie, Ling Lei, Wei Chen, Hailong Zhang, Man Chen, Xing Zhao, Xiufang Wan, Rui Yuan, Hongmei Jiang

“…KIF11 knockdown had inhibitory effects on cell proliferation, migration and invasion. Flow cytometry analysis revealed that KIF11 knockdown induced G2/M phase arrest and promoted apoptosis in EC cells. …”
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Combining theory and usability testing to inform optimization and implementation of an online primary care depression management tool by Nicola McCleary, Justin Presseau, Isabelle Perkins, Brittany Mutsaers, Claire E. Kendall, Janet Yamada, Katharine Gillis, Douglas Green

“…Usability issues were summarised within five themes: Optimizing content and flow to align with issues faced in practice, Enhancing the most useful algorithm components, Interactivity of the algorithm and embedded tools, Clarity of presence, purpose, or function of components, and Navigational challenges and functionality of links. …”
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CX3CR1+ age-associated CD4+ T cells contribute to synovial inflammation in late-onset rheumatoid arthritis by Mitsuhiro Akiyama, Sohma Wakasugi, Keiko Yoshimoto, Koichi Saito, Sho Ishigaki, Risa Inukai, Yoshiyuki Matsuno, Waleed Alshehri, Yasushi Kondo, Yuko Kaneko

“…Methods Fresh peripheral blood samples were collected from 78 treatment-naïve active RA patients, 12 with difficult-to-treat RA, and 16 healthy controls. Flow cytometry was employed to measure the proportions of CX3CR1+cytotoxic CD4+ and CD8+ T cells in these samples. …”
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Integration of artificial intelligence and advanced optimization techniques for continuous gas lift under restricted gas supply: A case study by Leila Zeinolabedini, Forough Ameli, Abdolhossein Hemmati-Sarapardeh

“…In the oil industry, gas lift is essential for facilitating fluid flow toward the production unit. However, the challenge lies in balancing gas availability constraints to achieve maximum efficiency in an oil field. …”
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On the lidar-turbulence paradox and possible countermeasures by A. Peña, G. G. Yankova, V. Mallini

“…<p>We describe the major difficulties in establishing a physics-based method that corrects lidar-based turbulence measures to enable adequate turbulence statistics of the atmospheric flow. The difficulties encompass the so-called lidar-turbulence paradox, which we circumvent in two ways. …”
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Ultrasonographic evaluation of joint involvement in rheumatoid arthritis: Comparison with conventional radiography and correlation with disease activity parameters by Renu Saigal, Laxmikant Goyal, Hariram Maharia, Meenakshi Sharma, Abhishek Agrawal

“…After X-ray imaging (posterior-anterior view) of both hand joints, PD and gray scale US examination of 14 joints of both hands was performed and mean cumulative flow signal score (CFS) was calculated. Disease activity score (DAS28) was also calculated for each patient. …”
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Characterization of Local Nano-Heat Transfer Fluids for Solar Thermal Collection by Kawira Millien

“…The heat capacity of the steam in the heat exchanger was 93.7% dry and had a thermal capacity of 5.71 × 103 kJ. The heat rate of flow of the oil-based copper nano-heat transfer fluids was an average of 72.7 Js−1·kg−1 to 89.1 Js−1·kg−1. …”
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The effects of ultra-selective beta1-antagonism on the metabolic and cytokine profile in septic shock patients receiving noradrenaline: a sub-investigation from the STRESS-L Random... by Jarrod L. Thomas, Kirsty C. McGee, Anower Hossain, Gavin D. Perkins, Anthony C. Gordon, Duncan Young, Danny McAuley, Mervyn Singer, Ranjit Lall, Tina Kramaric, Janet M. Lord, Tony Whitehouse, Luis A. J. Mur, for the STRESS-L. collaborators

“…Metabolite fingerprinting was carried out by flow infusion electrospray ionisation high-resolution mass spectrometry and metabolomic data were analysed using the R-based platform MetaboAnalyst. …”
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The Growing Role of Re-export in International Trade by V. N. Kirillov, Y. A. Savinov, A. E. Gudzenko

“…Specialized trading companies possess this knowledge; they create large transport and logistics centers in the countries crossing international trade routes, including extensive consignment warehouses.The article studies the dynamics of re-export flows and makes calculations, based on which it concludes that the volume of these commercial operations is growing faster in the world market. …”
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Proteomic Analysis of Hypoxia-Induced Senescence of Human Bone Marrow Mesenchymal Stem Cells by Liping Mai, Guodong He, Jing Chen, Jiening Zhu, Shaoxian Chen, Xinghua Hou, Hui Yang, Mengzhen Zhang, Yueheng Wu, Qiuxiong Lin, Min Yang, Xiaohong Li

“…Cell apoptosis was evaluated using Annexin V/7-AAD staining by flow cytometry. The production of reactive oxygen species (ROS) was detected by the fluorescent probe 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA). …”
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DNGR-1 limits Flt3L-mediated antitumor immunity by restraining tumor-infiltrating type I conventional dendritic cells by Ignacio Melero, Ruth Conde-Garrosa, David Sancho, Santos Mañes, Alfonso R Sanchez-Paulete, Francisco J Cueto, Carlos del Fresno, Paola Brandi, Alexis J. Combes, Elena Hernández-García, Michel Enamorado, Christian P Bromley, Manuel J Gomez, Santiago Zelenay, Salvador Iborra, Matthew F. Krummel

“…To characterize the immune response, we performed flow cytometry and RNA-Seq of tumor-infiltrating cDC1s.Results Here, we found that cross-presentation of tumor antigens in the steady state was DNGR-1-independent. …”
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Long Noncoding RNA HCG9 Promotes Osteosarcoma Progression through RAD51 by Acting as a ceRNA of miR-34b-3p by Lu Wang, ShuangQing Li, Lin Qi, Lin Ling

“…Colony formation assay was observed. Flow cytometry evaluated the cell cycle. Western blot was performed to detect the mitotic markers and apoptosis-related proteins. …”
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Pengenalan Jalan Berlubang Berbasis Vision Menggunakan Pyramid Histogram Of Oriented Gradients by Ahmad Habib Fitriansyah, Ema Rachmawati, Risnandar Risnandar

“…Preventative management of potholes is crucial for driver safety and efficient traffic flow. Traditional methods of pothole detection relied on visual inspection, but automatic methods have been developed using sensors and signal processing. …”
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The Prediction Model of High-Frequency Ultrasound Combined with Artificial Intelligence-Assisted Scoring System Improved the Diagnosis of Sclerosing Adenosis and Early Breast Cance... by Ma B, Wu G, Zhu H, Liu Y, Hu W, Zhao J, Liu Y, Liu Q

“…In the training group, the AUC of the sonographer and AI in differential diagnosis was 0.67(95% CI, 0.62– 0.71) and 0.89(95% CI, 0.84– 0.93), respectively, and the sonographer’s assessment showed better sensitivity (1.00 VS 0.73), but AI showed a higher accuracy rate (0.66 VS 0.80).Conclusion: Age, lesion size, burr, blood flow, and AI risk score are independent predictors of sclerosing adenosis and early breast cancer. …”
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CircHIPK2 recruits SRSF1 to increase TXNIP mRNA stability and promotes autophagy-dependent ferroptosis and apoptosis in myocardial ischemia-reperfusion injury by Zhu Zhang, Jinqi Hao, Qinghong Qiao, Junting Song, Yanqin Yu

“…Cell viability and apoptosis were assessed by CCK-8 and flow cytometry. Levels of oxidative stress markers (MDA, SOD) and inflammatory factors (IL-6, IL-1β, TNF-α) were tested by ELISA assay. …”
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Preparation of Iron Oxide and Titania-Based Composite, Core-Shell Populated, Nanoparticulates Material by Two-Step LASER Ablation in Aqueous Media as Antimicrobial and Anticancer A... by Hasan H. Bahjat, Raid A. Ismail, Ghassan M. Sulaiman, Hamdoon A. Mohammed, Mohsen Al-Omar, Salman A. A. Mohammed, Riaz A. Khan

“…The NPs prepared under the influence of the magnetic fields also comparatively exhibited higher levels of cytotoxicity against lung cancer cell lines (A549) than the NPs prepared under no magnetic field’s influence by the similar energy level effects of the LASER fluence. The flow cytometry analyses confirmed the NPs’ cytotoxic impacts against the human lung cancer A549 cell lines through the initiation of apoptosis and promotion of the cell cycle arrest at the G1 phase of cell division. …”
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Exploring caregiver experiences and support needs in end-of-life care for people living with HIV: A scoping review protocol. by Kristina M Kokorelias, Andrew D Eaton, Marina Wasilewski, Tyler Redublo, Luxey Sirisegaram

“…Final selections will be reported using the PRISMA flow diagram, and reasons for exclusion will be documented.…”
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Is There Any Point in a Corticosteroid Treatment of Intermittent Asthma? by Ivana Stankovic, Tatjana Pejcic, Branislava Milenkovic, Dragana Jovanovic, Milan Rancic

“…At the end of the study, in Group A, we found a statistically significant decrease of BHR (PD20 0.98 vs. 2.07) (p < 0.001), diurnal peak expiratory flow (PEF) variability (17.9 vs. 13.8) (p < 0.001), symptom scores (0.63 vs. 0.30) (p < 0.001), and used rescue medication (p < 0.001), while the parameters of lung function remained unchanged except for forced expiratory volume in 1 sec (FEV1), which had a statistically significant increase (3.58 vs. 3.66) (p < 0.001). …”
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