Matrine Inhibits Breast Cancer Cell Proliferation and Epithelial-Mesenchymal Transition Through Regulating the LINC01116/miR-9-5p/ITGB1 Axis by Lili Ren, Ziru Fang, Jiaojiao Xu, Xiaoxiao Wu, Yongjun Zhang, Hu Cai, Zhicun Han

“…Functional assays such as CCK-8, EdU, Transwell, Western blot, and flow cytometry assays were performed on the BC cells, and the impacts of LINC01116, miR-9-5p, and ITGB1 expression levels on MAT’s mechanism of action were assessed. …”
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lncRNA-KCNQ1OT1: A Potential Target in Exosomes Derived from Adipose-Derived Stem Cells for the Treatment of Osteoporosis by Shan-zheng Wang, Jun Jia, Chang-hong Chen

“…Cell viability and apoptosis were assessed by Cell Counting Kit-8 (CCK-8) and flow cytometry analysis, respectively. The binding sites between KCNQ1OT1 and miR-141-5p were validated by dual-luciferase reporter assay. …”
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Economic Feasibility of Ginger Emprit Extract Drink (Zingiber officinale Var. Amarum) using CMC Stabilizer by Ahmad Farhan, Anwar Kasim, Rini B

“…This parameter calculates projected production, revenue and cash flow. Determination of the economic feasibility of the Tempe juice drink with Emprit ginger extract with the Net Present Value (NPV), Incremental Rate of Return (IRR), Net B/C and Pay Back Period (PBP). …”
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Loop33 × 123 CAR-T targeting CD33 and CD123 against immune escape in acute myeloid leukemia by Haotian Ma, Zhifeng Yan, Runxia Gu, Yingxi Xu, Shaowei Qiu, Haiyan Xing, Kejing Tang, Zheng Tian, Qing Rao, Min Wang, Jianxiang Wang

“…Degranulation and cytokine secretion levels were detected by flow cytometry. Cell-derived xenograft models were established using wild-type Molm 13 cell lines, or a mixture of Molm 13-KO33 and Molm 13-KO123 cells as an ideal model of immune escape. …”
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Gasdermin D regulates the activation of EGFR in colorectal cancer by Ying Li, Jiayao Chen, Huijun Liang, Qindan Du, Jingjie Shen, Xiaoying Wang

“…GSDMD knockdown and overexpression stable cell lines were established by Lentiviral transduction. CCK-8 assay, flow cytometry analysis for cell cycle, Transwell assay, and cell scratch assay were performed in vitro to explore the impact of GSDMD on CRC progression. …”
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РЕЄСТРАЦІЯ ПРАВА ІНТЕЛЕКТУАЛЬНОЇ ВЛАСНОСТІ НА ОСНОВІ ТЕХНОЛОГІЇ БЛОКЧЕЙН by Максим Вінник, Максим Полторацький, Ольга Коннова, Наталія Кушнір, Андрій Богданович

“…Нами було реалізовано смарт-контракт, який керує процесом створення та передачі  NFT з використанням мови Cadence на блокчейні Flow. Крім того, було реалізовано ключові функціональні вимоги до системи, а саме: реєстрація авторів та експертів (рецензентів), додання ключових слів для відбору експертів (рецензентів), пошук відповідних експертів за ключовими словами, реалізація функцій оплати рецензентам за виконану роботу. …”
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Human development II: We Need an Integrated Theory for Matter, Life and Consciousness to Understand Life and Healing by Sören Ventegodt, Tyge Dahl Hermansen, Maj Lyck Nielsen, Birgitte Clausen, Joav Merrick

“…The problems we address are all connected to the information flow in the functioning, living organism: function of the brain and consciousness, the regulations of the immune system and cell growth, the dynamics of health and disease. …”
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Genetic differentiation and historical dynamics of the endemic species Rheum pumilum on the Qinghai-Tibetan Plateau inferred from phylogeography implications by Shuliang Liu, Jianxin Gao, Bo Xiao, Wenjie Guo, Qiushi Yu, Ailan Wang, Weiwei Li

“…The total genetic diversity of Rh. pumilum is remarkably high (Ht = 0.910), with the majority of genetic variation primarily occurred among populations (84.5%) with limited gene flow, indicating geographic isolation influenced by diverse habitats of plateau. …”
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Preparation of noninfectious scRNAseq samples from SARS-CoV-2-infected epithelial cells. by Raven M Osborn, Justin Leach, Michelle Zanche, John M Ashton, ChinYi Chu, Juilee Thakar, Stephen Dewhurst, Sonia Rosenberger, Martin Pavelka, Gloria S Pryhuber, Thomas J Mariani, Christopher S Anderson

“…Fixed cells were tested for the presence of infectious SARS-CoV-2 virions using the Tissue Culture Infectious Dose Assay (TCID50), and also tested for viability using flow cytometry. We then tested the dissociation and methanol-acetone inactivation method on primary human lung epithelial cells that had been differentiated on an air-liquid interface. …”
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Selective expression and significance of ACKR2 in lung aerocytes by Alberto Mantovani, Gianluigi Condorelli, Raffaella Bonecchi, Massimo Locati, Marina Sironi, Roberta Carriero, Francesca Albano, Valeria Mollica Poeta, Lucia Zotti, Alessandra Castagna, Arianna Felicetta, Alessandro Mesaglio, Elisa Zaghen, Arianna Capucetti, Rachele Di Donato, Mattia Laffranchi, Matteo Massara, Silvano Sozzani

“…This study aims to explore the specific cell types expressing ACKR2 and their relative contributions to the protection against lung metastasis.Methods ACKR2 expression was studied by the generation of an inducible and conditional knockout (KO) mouse expressing two reporter genes, luciferase and TdTomato visible by In Vivo Imaging System, flow cytometry and immunofluorescence. Gene expression in lung endothelial cells (ECs) was investigated by RNA sequencing analysis. …”
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The temporal variation of CH4 emissions embodied in Chinese supply chains, 2000–2020 by Jiaxi Wu, Mengxin Chen, Xialing Sun, Zheng Meng

“…Notably, the high-ranked path, “Agriculture → Food and tobacco → Rural consumption”, shows that the embodied CH4 emission flowing between agriculture and the food industry cannot be ignored. …”
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Interpreting machine learning models based on SHAP values in predicting suspended sediment concentration by Houda Lamane, Latifa Mouhir, Rachid Moussadek, Bouamar Baghdad, Ozgur Kisi, Ali El Bilali

“…The interpretability results showed that flow (Q) and seasonality (S) are the features most impacting SSC. …”
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Design and Evaluation of a Management Dashboard for a Children\'s Subspecialty Hospital: An Action Research Approach by Malihe Ghanaatjoo, Nader Jahanmehr, Hamed Dehnavi, Aida Samadi

“…Conclusion: Hospital management dashboard information can be a basis for informed decision-making to achieve benefits such as identifying the best performance, improving performance quality, making faster decisions, reducing errors, improving capacity management and work flow, allocating resources and planning for growth and development.…”
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Effects of bronchoscopic alveolar lavage–assisted mechanical ventilation on postoperative pulmonary infection and inflammatory factors in patients undergoing lung cancer surgery by Hui Zhu, Haiyang Lu

“…Maximum minute ventilation, total lung capacity, forced expiratory volume in 1 second, and maximum mid-expiratory flow were substantially enhanced following each treatment. …”
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Hypothermia regulates mitophagy and apoptosis via PINK1/Parkin-VDAC 3 signaling pathway during oxygen-glucose deprivation/recovery injury by Luying Zhang, Song Yang, Hao Cui, Chenchen Hang, Xingsheng Wang, Le An, Zhenyu Shan, Zhen Liang, Rui Shao, Ziren Tang

“…Mitochondrial membrane potential (MMP) and mitochondrial permeability transition pore (mPTP) opening were determined respectively by JC-1 staining and BBcellProbe M61 staining using a flow cytometer. Expression of mitophagy-related proteins (Cleaved PINK1, Parkin, SQSTM1/p62, Beclin-1, LC3B II/LC3B I), apoptosis-related proteins (Bcl-2, Cytochrome C, caspase-3, cleaved caspase3) and VDAC3 were assessed using western blot analysis and quantitative real-time PCR. …”
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The Influence of Coupled Thermomechanical Processes on the Pressure and Temperature due to Cold Water Injection into Multiple Fracture Zones in Deep Rock Formation by Bruno Figueiredo, Chin-Fu Tsang, Auli Niemi

“…Changes in temperature were found not to be affected significantly by the thermomechanical and hydromechanical effects, but by the flow rate in the fracture zones. A sensitivity analysis was conducted to study the influence of the number, the initial permeability, the elastic modulus and the residual porosity of the fracture zones, and the elastic modulus of the confining intact rock, on the simulation results. …”
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Introducing an index on prediction of post-revascularization cerebral infarction using preoperative CT perfusion parameters in moyamoya disease by Xiaojun Hao, Chao Zhang, Chen Yang, Xintong Zhao, Yunfeng Zhou, Juan Wang

“…Preoperative quantitative CTP parameters, including cerebral blood flow (CBF), cerebral blood volume (CBV), mean transit time (MTT), time to drain (TTD), and transit time to maximum of the residue function (Tmax), along with clinical data, were compared between the groups with and without post-CI. …”
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Amantadine modulates novel macrophage phenotypes to enhance neural repair following spinal cord injury by Shijie Yang, Beibei Yu, Qing Zhang, Yongfeng Zhang, Longhui Fu, Bisheng Zhou, Haining Wu, Jianzhong Li, Shouping Gong

“…Subsequently, immunofluorescence staining, flow cytometry, and western blotting were performed to validate the identified effects of amantadine on macrophage differentiation and inflammation. …”
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Impact of Renewable Energy Sources on the Electricity Generation Cost by V. A. Liubchyk

“…The study involves scenario modeling based on the construction of energy chains that determine the flow of energy from the extraction and import of energy resources through technologies for transforming energy forms, transmission and distribution to the final consumer. …”
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Displacement of hydrocarbon liquids by water using the models of zonally heterogeneous deformable formations by Marat Ya. Khabibullin, Rustem I. Suleymanov, Razifa R. Stepanova, Alina Az. Gizzatullina, Arsen M. Khabibullin

“…In this paper, the averaging method and the Khristianovich function will be used to linearize the equations. To predict the well flow rate, an algorithm it is necessary to determine the noted reservoir parameters at any time. …”
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