Pathological manifestations in lymphatic filariasis correlate with lack of inhibitory properties of IgG4 antibodies on IgE-activated granulocytes. by Ulrich F Prodjinotho, Charlotte von Horn, Alex Y Debrah, Linda Batsa Debrah, Anna Albers, Laura E Layland, Achim Hoerauf, Tomabu Adjobimey

“…The activation and degranulation states were investigated by monitoring the expression of CD63/HLADR and the release of granule contents (neutrophil elastase (NE), eosinophil cationic protein (ECP) and histamine) respectively by flow cytometry and ELISA. We could show that the activation of granulocytes was inhibited in the presence of plasma from EN and Mf+ individuals whereas those of Mf- and CP presented no effect. …”
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Cytotoxicity Potential of Endophytic Fungi Extracts from Terminalia catappa against Human Cervical Cancer Cells by Michèle Stella Majoumouo, Marius Belmondo Tincho, Rufin Marie Kouipou Toghueo, Thureyah Morris, Donavon Charles Hiss, Fabrice Fekam Boyom, Chitra Mandal

“…Furthermore, apoptosis was determined using the Annexin V/propidium iodide (PI) flow cytometry assay. Endophyte extracts N2, N7, N8, N97, N169, and N233 were obtained from Trichoderma sp, Phoma sp, Phomopsis phyllanticola, Fusarium oxyporum, Collectotrichum sp, and Cryptococcus flavescens, respectively. …”
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CircBAZ1B stimulates myocardial ischemia/reperfusion injury (MI/RI) by modulating miR-1252-5p/ATF3-mediated ferroptosis by Ruili Wei, Tianxiao Yang, Weihong Li, Xiqian Wang

“…Cell viability, apoptosis, lipid reactive oxygen species (ROS) levels and iron content were determined via Cell Counting Kit-8 (CCK-8) and flow cytometric assays. Gene and ferroptosis-related protein expression levels were verified by qRT‒PCR and Western blotting. …”
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Th9 Cells in Peripheral Blood Increased in Patients with Immune-Related Pancytopenia by Qing Shao, Yangyang Wang, Zhaoyun Liu, Hui Liu, Yihao Wang, Yang Zhao, Lijuan Li, Rong Fu

“…The percentage of Th9 cells was detected by flow cytometry (FCM) and ELISA. CD4+ lymphocytes were sorted by magnetic beads, and the mRNA expression levels of Th9 cells related transcription factors PU.1 and BATF were detected by RT-PCR. …”
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Alpha-synuclein pathology enhances peripheral and CNS immune responses to bacterial endotoxins by Anna-Sophia Hartke, Cara S. Schreiber, Kristina Lau, Ivo Wiesweg, Inken Waltl, Ulrich Kalinke, Franziska Richter, Christopher Käufer

“…To investigate this, we created a double-hit model based on the Thy1-aSyn mouse line (line 61), an established aSyn-overexpression model of PD, exposed to a single intraperitoneal injection of LPS at a dose of 0.8 mg/kg (equivalent to approximately 1,200,000 EU/kg). Clinical parameters, flow cytometry of blood and immune cells in the brain, brain immunohistology and motor behavior were evaluated over time. …”
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A cross-sectional study on the correlation between internal cerebral vein asymmetry and hemorrhagic transformation following endovascular thrombectomy by Kunxin Lin, Kunxin Lin, Wenlong Zhao, Wenlong Zhao, Quanhong Wu, Quanhong Wu, Yiru Zheng, Yiru Zheng, Bo Yang, Ying Fu, Ying Fu, Ning Wang, Ning Wang, Ling Fang, Ling Fang

“…In multivariate logistic regression, ICV asymmetry (OR 3.809, 95% CI 1.582–9.171), baseline Alberta Stroke Program Early CT Score (OR 0.771, 95% CI 0.608–0.978), intravenous recombinant tissue plasminogen activator (OR 2.847, 95% CI 1.098–2.7.385), and poor collateral circulation (OR 3.998, 95% CI 1.572–10.169) were identified as independent risk factors of HT.ConclusionICV asymmetry, likely resulting from impaired autoregulation or tissue micro-perfusion hampering cerebral blood flow (CBF), is a novel radiological sign that independently predicts HT. …”
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Age‐related longitudinal change in cardiac structure and function in adults at increased cardiovascular risk by Fei Fei Gong, Jennifer M. Coller, Michele McGrady, Umberto Boffa, Louise Shiel, Danny Liew, Simon Stewart, Alice J. Owen, Henry Krum, Christopher M. Reid, David L. Prior, Duncan J. Campbell

“…Age was associated with longitudinal increase in left ventricular (LV) mass index (LVMI); decrease in LV volumes; increase in LV ejection fraction; decrease in mitral annular systolic velocity; decrease in diastolic function (decreased mitral early diastolic annular velocity (e′); and increase in left atrial volume index, mitral peak early diastolic flow velocity (E)/e′ ratio, and tricuspid regurgitant velocity (TRVmax) in men and women, except for TRVmax in men). …”
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Exponential Growth and Properties of Solutions for a Forced System of Incompressible Navier–Stokes Equations in Sobolev–Gevrey Spaces by José Luis Díaz Palencia

“…This analysis in intended to contribute in the understanding of the behavior of fluid flows with forcing terms in high-regularity function spaces.…”
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Cell culture expansion media choice affects secretory, protective and immuno-modulatory features of adipose mesenchymal stromal cell-derived secretomes for orthopaedic applications by Enrico Ragni, Andrea Papait, Michela Maria Taiana, Paola De Luca, Giulio Grieco, Elsa Vertua, Pietro Romele, Cecilia Colombo, Antonietta Rosa Silini, Ornella Parolini, Laura de Girolamo

“…Methods: Immunophenotype and secretory ability at soluble protein and EV-related levels, including embedded miRNAs, were analysed in the secretomes by means of flow cytometry, nanoparticle tracking analysis, high throughput ELISA and qRT-PCR arrays. …”
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Mapping factors influencing initiation of antiretroviral treatment among adolescents living with HIV/AIDS in sub-Saharan Africa: A scoping review protocol. by Palesa Grace Likoti, Desmond Kuupiel, Nelisiwe Khuzwayo

“…The selection process will be documented by following and using the PRISMA flow diagram (Fig 1). A thematic content analysis will present a narrative account of the extracted data.…”
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The Use of Trichostatin A during Pluripotent Stem Cell Generation Does Not Affect MHC Expression Level by Sara Farahi, Sara Hosseini, Hossein Ghanbarian, Seyed Mahmoud Hashemi, Mohammad Salehi, Samaneh Hosseini

“…The presence of MHC molecules on the surface of pluripotent stem cells was also checked by flow cytometry technique. Significant increase of pluripotency markers, oct4, nanog, sox2, and klf4, was observed in 100 nM TSA-treated samples. 100 nM TSA induced significant upregulation of H2db in generated iPSCs. …”
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Research Participants’ Engagement and Retention in Digital Health Interventions Research: Protocol for Mixed Methods Systematic Review by Luciana Terceiro, Mudassir Imran Mustafa, Maria Hägglund, Anna Kharko

“…Data analysis and synthesis will follow the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) 2020 flow diagram. Quantitative data will be qualified and integrated into qualitative data, which will be analyzed using thematic analysis and synthesis. …”
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Empagliflozin-Metformin Combination Has Antioxidative and Anti-Inflammatory Properties that Correlate with Vascular Protection in Adults with Type 1 Diabetes by Miodrag Janić, Matej Cankar, Jan Šmid, Alenka France Štiglic, Aleš Jerin, Mišo Šabovič, Andrej Janež, Mojca Lunder

“…., it improved endothelial function (measured by flow-mediated dilation and reactive hyperaemia index) and reduced arterial stiffness (measured as pulse wave velocity and local carotid artery stiffness) in adults with type 1 diabetes. …”
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Circ-PDE1C/miR-766-3p/SGTB axis regulates the IL-1β-induced apoptosis, inflammation and oxidative stress in human chondrocytes by Lixia Gao, Tao He, Qingkui Hu, Yan Ma

“…Cell counting kit-8 (CCK-8) assay and flow cytometry were used to analyze proliferation and apoptosis, respectively. …”
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Development of a TCR-like antibody and chimeric antigen receptor against NY-ESO-1/HLA-A2 for cancer immunotherapy by Xin Liu, Yang Du, Ningyan Zhang, Zhiqiang An, Wei Xiong, Yixiang Xu, Bingnan Yin, Yuqian Huang, Junjun Chu, Changsheng Xing, Chen Qian, Tianhao Duan, Helen Y Wang, John S. Yu, Rongfu Wang

“…The specificity and affinity of those antibodies were characterized by flow cytometry, ELISA, biolayer interferometry, and confocal imaging. …”
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Associer les acteurs de terrain à l’observation de la fréquentation et des pratiques récréatives en montagne : enseignements à partir de deux projets collaboratifs en France et en... by Christophe Clivaz, Marc Langenbach, Olivier Obin, Alexandre Savioz

“…The results in terms of data collected or issues identified (changes in visitor flows, the role of hut keepers, types of clientele, etc.) are not part of the subject of this contribution, even if they are mentioned from time to time in order to illustrate the topic.Our methodology is based on the final report of the participatory research-action project we conducted (Obin et al., 2020), on a student thesis on the feasibility of the Sentinel Hut program and of the approach to be implemented (Berthet, 2014), as well as on the minutes of various sessions and events related to this program. …”
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One-Step Solid Extraction for Simultaneous Determination of Eleven Commonly Used Anticancer Drugs and One Active Metabolite in Human Plasma by HPLC-MS/MS by Shouhong Gao, Zhengbo Tao, Jingya Zhou, Zhipeng Wang, Yunlei Yun, Mingming Li, Feng Zhang, Wansheng Chen, Yejun Miao

“…Chromatographic separation was achieved on an Atlantis T3-C18 column (2.1 × 100 mm, 3.0 μm) with gradient elution using a mobile phase consisting of acetonitrile and 10 mM ammonium acetate plus 0.1% formic acid in water, and the flow rate was 0.25 mL/min. The Agilent G6410A triple quadrupole liquid chromatography-mass spectrometry system was operated under the multiple reaction monitoring mode with an electrospray ionization in the positive mode. …”
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Therapeutic Effect of Gypenosides on Antioxidant Stress Injury in Orbital Fibroblasts of Graves’ Orbitopathy by Chao Ma, Haoyu Li, Wei Liu, Shuwen Lu, Xian Li, Jinyuan Chen, Kaijun Li, Wenzhan Wang

“…ROS and SOD test suites were employed to detect the oxidative stress level in OFs. Flow cytometry apoptosis detection, TUNEL detection, and lactate dehydrogenase detection were used to analyze the level of apoptosis. …”
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Research on leakage and diffusion behavior of hydrogen doped natural gas in integrated pipeline corridors based on data drive by Xiaolong Gong, Hua Li, Cheng Li, Mingyue Kou, Lingxu Kong, Hongcheng Liu

“…In the case of emergency, the reasonable design of Distance between inlet and outlet and air flow configuration can help to quickly discharge harmful gases, ensure the safe evacuation and equipment maintenance in the pipe corridor, and have guiding significance for the ventilation design of the pipe corridor.…”
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Soil phosphorus mobilization as affected by long-term organic fertilization in a tropical Regosol by Kalline De Almeida Alves Carneiro, Tancredo Augusto Feitosa De Souza, Gislaine Dos Santos Nascimento, Vânia Da Silva Fraga, Bruno de Oliveira Dias

“…This study aimed to evaluate the PM in a tropical Regosol by using soil columns (10 cm depth) during steady saturated water flow and PM was correlated with Fe and Al oxides. …”
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