Targeting sphingosine 1-phosphate receptor 3 inhibits T-cell exhaustion and regulates recruitment of proinflammatory macrophages to improve antitumor efficacy of CAR-T cells agains... by Weiting Liao, Ge Gao, Xia He, Pei Shu, Qizhi Ma, Benxia Zhang, Diyuan Qin, Yongsheng Wang

“…We have applied RNA sequencing, flow cytometry, ELISA, cellular/molecular immunological technology, and mouse models of solid cancers.Results Our study provided evidence that S1PR3 high expression is positively associated with resistance to programmed cell death protein-1 (PD-1)-based immunotherapy and increased T-cell exhaustion. …”
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Investigating Elevated E-Selectin and P-Selectin Levels in Non-Arteritic Anterior Ischemic Optic Neuropathy (NAION) Patients: The Stepping Stone to a Future Clinical Approach by Syntia Nusanti, Andhika Rachman, Brigitta Marcia Budihardja, Lourisa Ruth Eldinia, Nadia Delima Andini, Arief Kartasasmita, M Sidik, Seskoati Prayitnaningsih, Alida Roswita Harahap, Aria Kekalih, Tjahjono Darminto Gondhowiardjo

“…All patients were evaluated for E-selectin and P-selectin levels measured using flow cytometry.  Results: A total of 42 subjects comprising 14 hypercoagulation and 28 non-hypercoagulation subjects were included. …”
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Dual‐fluorescence imaging and automated trophallaxis detection for studying multi‐nutrient regulation in superorganisms by Lior Baltiansky, Einav Sarafian‐Tamam, Efrat Greenwald, Ofer Feinerman

“…More broadly, the ability to simultaneously and efficiently track two material flows in a behaving colony provides new opportunities for tackling many open and emerging questions on topics other than nutrition, such as disease spread and social regulation of colony development.…”
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Efficacy of the Bushen Jianpi Huoxue Formula on Beclin-1/Bcl-2-mediated autophagy and apoptosis in osteoblasts by Yanping Lin, Yanping Lin, Rui Zhao, Rui Zhao, Jiachun Huang, Tongying Chen, Tongying Chen, Haolin Yang, Haolin Yang, Haiwei Guo, Lei Wan, Zhihai Zhang, Ying Li, Genfu Zhu, Hongxing Huang, Hongxing Huang

“…Cell viability, apoptosis, and autophagy were assayed with CCK-8, flow cytometer, MDC staining, and Lyso-Tracker staining, respectively. …”
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Speciation Analysis of Arsenic Compounds by High-Performance Liquid Chromatography in Combination with Inductively Coupled Plasma Dynamic Reaction Cell Quadrupole Mass Spectrometry... by Hai Anh Vu, Manh Ha Nguyen, Hong-An Vu-Thi, Quan Do-Hong, Xuan Hoang Dang, Thi Ngoc Bich Nguyen, Hong Quan Trinh, Thuy Ly Bich, Tien-Thanh Nguyen, Dung Le-Van, Minh Binh Tu, Dinh Binh Chu

“…Arsenic compounds were detected online by inductively coupled plasma dynamic reaction cell quadrupole mass spectrometry utilizing oxygen as the reaction gas at a flow rate of 0.7 mL·min−1. Five selected arsenic species were baseline separated at the optimum experimental conditions. …”
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Integrating biomarkers for hemostatic disorders into computational models of blood clot formation: A systematic review by Mohamad Al Bannoud, Tiago Dias Martins, Silmara Aparecida de Lima Montalvão, Joyce Maria Annichino-Bizzacchi, Rubens Maciel Filho, Maria Regina Wolf Maciel

“…Eligibility criteria included mathematical models of thrombin generation, blood clotting, or fibrin formation under flow, incorporating at least one of these biomarkers. …”
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Role of Toll-Like Receptor 2 in Regulation of T-Helper Immune Response in Chronic Obstructive Pulmonary Disease by Karolina A. Sidletskaya, Tatyana I. Vitkina, Yulia K. Denisenko, Elena E. Mineeva

“…Serum levels of Th1 (TNF-α and IFN-γ), Th2 (IL-4), Th17 (IL-6 and IL-17A), Treg (IL-10) cytokines, and the percentage of peripheral blood Th cells expressing TLR2 (CD4+CD282+) were assessed by flow cytometry. Serum concentrations of IL-21 (Th17) and TGF-β1 (Treg) were measured using the ELISA method. …”
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Th2-Immune Polarizing and Anti-Inflammatory Properties of Insulin Are Not Effective in Type 2 Diabetic Pregnancy by Adnette Fagninou, Magloire Pandoua Nekoua, Darius Sossou, Kabirou Moutairou, Nadine Fievet, Akadiri Yessoufou

“…Immune cell subpopulation frequencies were determined in blood using flow cytometry. The proliferative capacity of T cells was performed, and serum and cell culture supernatant cytokine levels were also quantified. …”
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Effect of noninvasive respiratory support on interstitial lung disease with acute respiratory failure: A systematic review and meta-analysis by Natthawan Sanguanwong, Nattawat Jantarangsi, Jinjuta Ngeyvijit, Natthida Owattanapanich, Vorakamol Phoophiboon

“…# Background Primary studies have demonstrated the effectiveness of noninvasive respiratory supports, including noninvasive positive pressure ventilation (NIPPV) and high flow nasal cannula (HFNC), for improving oxygenation and ventilation in patients with interstitial lung diseases (ILDs) and acute respiratory failure (ARF). …”
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Multi-scale hydraulic graph neural networks for flood modelling by R. Bentivoglio, E. Isufi, S. N. Jonkman, R. Taormina

“…First, they cannot model rapid differences in flow propagation speeds; secondly, they can face instabilities during training when using a large number of layers, needed for effective modelling; third, they cannot accommodate time-varying boundary conditions; and fourth, they require initial conditions from a numerical solver. …”
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Evaluation of B cell related markers and autoantibodies in rheumatoid arthritis patients treated with abatacept by Ting Wang, Natalia V. Giltiay, Christian Lood, Ning Wang, Bobby Kwanghoon Han

“…Peripheral blood mononuclear cells were isolated and analysed for T cell and B cell subsets by flow cytometry. Patients were genotyped for human leukocyte antigen (HLA)-DRB1 shared epitope (SE) alleles. …”
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Differentiation between glioblastoma and solitary brain metastases using perfusion and amide proton transfer weighted MRI by Malte Knutsson, Tim Salomonsson, Faris Durmo, Emelie Ryd Johansson, Anina Seidemo, Jimmy Lätt, Anna Rydelius, Sara Kinhult, Elisabet Englund, Johan Bengzon, Peter C. M. van Zijl, Peter C. M. van Zijl, Linda Knutsson, Linda Knutsson, Linda Knutsson, Pia C. Sundgren, Pia C. Sundgren, Pia C. Sundgren

“…The objective of this study was to assess whether amide proton transfer weighted (APTw) MRI alone or combined with dynamic susceptibility contrast (DSC) MRI parameters, including cerebral blood volume (CBV), cerebral blood flow (CBF), and leakage parameter (K2) measurements, can differentiate GBM from MET.MethodsAPTw MRI and DSC-MRI were performed on 18 patients diagnosed with GBM (N = 10) or MET (N = 8). …”
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ESAT-6 protein suppresses allograft rejection by inducing CD4+Foxp3+ regulatory T cells through IκBα/cRel pathway by Xiaofei Huang, Xiaofei Huang, Yuqun Zeng, Jingru Lin, Huazhen Liu, Huazhen Liu, Chun-Ling Liang, Chun-Ling Liang, Yuchao Chen, Yuchao Chen, Feifei Qiu, Feifei Qiu, Jonathan S. Bromberg, Zhenhua Dai, Zhenhua Dai

“…ESAT-6 may be a potential alternative immunosuppressant that could be utilized to suppress allograft rejection although it remains unknown whether ESAT-6 actually regulates alloimmunity.MethodsIn this study, murine skin or heart allotransplantation was performed to determine the effects of ESAT-6 protein on allograft survival. Flow cytometric analyses were conducted to quantify CD4+Foxp3+ Tregs, while immunohistochemistry was carried out to observe allograft immunopathology. …”
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Spirituality-Based Intervention in Hypertension: EFfects on Blood PrEssure and EndotheliaL Function—FEEL Trial Results by Maria Emília Figueiredo Teixeira, Weimar Kunz Sebba Barroso, Andréa Araújo Brandão, Ana Luiza Lima Sousa, Roberto Esporcatte, Mário Henrique Elesbão de Borba, Ana Clara Neri Ávila Baleeiro, Beatriz Caldas Gonçalves, Enzo Inumaru, Enzo Mata de Sousa, Giovana Barros Leal, Henrique Soares de Araújo Pereira Farias, Juliana Alves de Souza, Lure Emilly Barreto da Silva, Matheus Canguçu de Paiva Queiroz, Frederico Rafael Moreira, Priscila Valverde de Oliveira Vitorino, John Eikelboom, Álvaro Avezum

“…Following baseline evaluation, including lifestyle questionnaires, and measurements of office and central blood pressure (BP), home blood pressure monitoring (HBPM) and flow mediated dilation (FMD), patients were randomized to a spirituality-based intervention, which included training for forgiveness, gratitude, optimism, and life purpose delivered by daily WhatsApp communications, or to the control group (CG). …”
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Study on the Key Influential Factors on Water Huff-n-Puff in Ultralow-Permeability Reservoir by Xiaoyan Wang, Kun Xie, Jie Zhang, Yong Zhang, Yang Zhang, Wei Wang, Xi Yan, Fengxiang Zhao

“…Higher reservoir energy is good for oil flow, and better physical property can improve the displacement effect and imbibition function of injection solution. …”
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Research on Territorial Spatial Use Regulation, Land Element Allocation, and Regional Fiscal Transfer Payments: An Empirical Study of the Yangtze River Economic Belt by Wanmin Zhao, Yijia Gao, Aihui Ma

“…The regulation of land use within territorial spaces has, to some extent, inhibited the free flow of land resources, giving rise to the dilemma of substantial losses and profits within and outside regulated areas. …”
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Curdione combined with borneol treats bacterial mixed HPV infection by regulating the crosstalk among immune cells by Jingwei Liu, Jingwei Liu, Tong Shu, Yiheng Mu, Wanlin Zheng, Xiaohuan Lu, Xiaohuan Lu, Hong Tao, Hong Tao

“…Bioinformatics and network pharmacology approaches were employed to identify therapeutic targets of Baofukang suppository for BV/HPV co-infections. qRT-PCR, Western blot, immunofluorescence staining, and flow cytometry were utilized to validate the therapeutic targets of curdione and borneol, along with the associated immune molecular changes. …”
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Citizen science platforms can effectively support early detection of invasive alien species according to species traits by Pablo González‐Moreno, Ana A. Anđelković, Tim Adriaens, Christophe Botella, Jakovos Demetriou, Rita Bastos, Sandro Bertolino, Celia López‐Cañizares, Franz Essl, Živa Fišer, Milka Glavendekić, Marc Herremans, Philip E. Hulme, Viola Jani, Dimitra Katsada, Periklis Kleitou, Nicola La Porta, Katharina Lapin, Marta López‐Darias, Vanessa Lozano, Angeliki F. Martinou, Damiano Oldoni, Esra Per, Stephanie Rorke, Helen E. Roy, Karl‐Manfred Schweinzer, Kristijn Swinnen, Elena Tricarico, Joana R. Vicente, Maarten de Groot, Michael J. O. Pocock

“…We recommend that IAS surveillance programmes should be better connected with citizen science platforms, including greater acknowledgement of the role of citizen scientists and better data flow from smaller citizen science initiatives into global databases, to support efficient early detection. …”
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S100A13-driven interaction between pancreatic adenocarcinoma cells and cancer-associated fibroblasts promotes tumor progression through calcium signaling by Liuyuan Xia, Xin Guo, Dong Lu, Yixin Jiang, Xiaohui Liang, Yiwen Shen, Jiayi Lin, Lijun Zhang, Hongzhuan Chen, Jinmei Jin, Xin Luan, Weidong Zhang

“…Cellular viability and apoptosis rates were evaluated through CCK-8 and flow cytometry tests, respectively. Additionally, the wound healing and migration assays were conducted to assess the invasive and metastatic capabilities. …”
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Monocyte and Macrophage in Follicular Liquid: Predictive Markers of Embryo Quality in Women with Obesity and Infertility by Evgeny D. Merkulov, Anastasia V. Prozorova, Iuliia G. Samoilova, Dmitry A. Svarovsky, Kira A. Sidorenkova, Liudmila V. Spirina, Marina N. Stakheeva, Oxana S. Timofeeva, Ilya A. Petrov

“…Monocytes and macrophages were identified using flow cytometry. …”
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