PPARα suppresses growth of hepatocellular carcinoma in a high-fat diet context by reducing neutrophil extracellular trap release by Banglun Pan, Zhu Zhang, Dongjie Ye, Xiaoxia Zhang, Yuxin Yao, Yue Luo, Haijie Hong, Xinran Cai, Yanling Chen, Nanhong Tang

“…Methods: In this study, the molecular mechanism of NET release and the potential beneficial effects of PPARα agonists on the HCC microenvironment were explored through proteomics, metabolomics, tissue microarray, immunofluorescence, flow cytometry, western blot, and dual-luciferase reporter gene assays (n = 6 per group). …”
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Mechanistic insights into Y-Box binding protein-1 mediated regulation of lipid metabolism and oxidative stress in NAFLD via INHBE/TNF-β pathway by Zhi Ren, Rui Wang, Jun Wei, Zhenzeng Ma, Xiquan Ke

“…Quantitative real-time PCR (QRT-PCR), Western blotting, flow cytometry, and various biochemical assays were used to evaluate gene expression, lipid accumulation, and oxidative stress. …”
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Association of player position and functional connectivity alterations in collegiate American football players: an fMRI study by Owen Griffith, Xiaoxiao Bai, Alexa E. Walter, Michael Gay, Jon Kelly, Wayne Sebastianelli, Linda Papa, Semyon Slobounov

“…IntroductionResting state-fMRI, provides a sensitive method for detecting changes in brain functional integrity, both with respect to regional oxygenated blood flow and whole network connectivity. The primary goal of this report was to examine alterations in functional connectivity in collegiate American football players after a season of repetitive head impact exposure.MethodsCollegiate football players completed a rs-fMRI at pre-season and 1 week into post-season. …”
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Interface Acoustic Waves in 128° YX-LiNbO₃/SU-8/Overcoat Structures by Cinzia Caliendo, Massimiliano Benetti, Domenico Cannatà, Farouk Laidoudi, Gaetana Petrone

“…Indeed, the IAWs may represent a valid alternative to standing SAWs, which are strongly attenuated when travelling beneath the walls of polydimethylsiloxane (PDMS) microfluidic channels for continuous flow particle manipulation, provided that the channel is excavated into the overcoating.…”
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Discrete Element Modeling on Mechanical Behavior of Heterogeneous Rock Containing X-Shaped Fissure under Uniaxial Compression by Ming Chen, Jian Liu, Zhengyong Xie, Jianjun Liu, Xunjian Hu, Bingyang Li, Yue Cen

“…Based on the experimental results of an intact rock specimen under uniaxial compression, particle flow code (PFC2D) was adopted to carry out a discrete element modeling (DEM) for the mechanical behavior of heterogeneous rocks containing X-shaped fissures (two intersecting symmetric single fissures) under uniaxial compression. …”
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Genetic Variation and Bottleneck Tests in Iraqi Native Cows of Babylon Province by STR Markers by Hayder R. Alnajm, Talib A. Alrubaye, Arash Javanmard

“…The mean Fis, Fit and Fst, and gene flow (Nm) were 0.296 ± 0.065, 0.314 ± 0.090, 0.032 ± 0.002, and 7.259 ± 2.113 respectively.  …”
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Geology of the Tabakoto gold deposit, Kédougou-Kéniéba Inlier, West African Craton, Mali by Diallo Mahamadou, Salvi Stefano, Baratoux Lenka, Béziat Didier, Vanderhaeghe Olivier, Labou Ibrahima, Baratoux David, Ly Saïdou

“…The litho-structural and mineralogical features of the Tabakoto deposit portray a polyphase mineralization with (i) an intrusion-hosted orogenic gold system, where the metabasltic to metarhyolitic dikes acted as suitable emplacement for fluids flow and (ii) a hydrothermal fluid circulation under ductile to brittle conditions.…”
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Development of algorithm for calculation of operation and repair efficiency of a plate heat exchanger by S. M. Chekardovsky, K. N. Ilyukhin, A. A. Melekhin, M. N. Chekardovsky

“…The calculation results were used to determine the exergy losses of the designed heat exchanger using the calculated values of the average logarithmic temperatures and other parameters of the heating and heated heat carriers; exergy losses from heat exchange with the environment, from the final temperature difference of heat carriers, from hydraulic resistances in the paths of heat carriers; specific thermal exergy of heat carriers at the inlet and outlet of the heat exchanger; exergy flow at the inlet and outlet of the apparatus; exergy coefficient of the heat exchanger. …”
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Aspergillus fumigatus extract modulates human eosinophils via NOD2 and oxidative stress by Hisashi Sasaki, Jun Miyata, Yusuke Kawashima, Ryo Konno, Masaki Ishikawa, Yoshinori Hasegawa, Ryuta Onozato, Yo Otsu, Emiko Matsuyama, Keeya Sunata, Katsunori Masaki, Hiroki Kabata, Yoshifumi Kimizuka, Tomoe Abe, Shigeharu Ueki, Koichiro Asano, Akihiko Kawana, Koichi Fukunaga

“…The expression of specific factors was evaluated using quantitative real-time polymerase chain reaction and flow cytometry. Mechanistic analyses were performed using NOD2 inhibitor and N-acetyl-l-cysteine (NAC). …”
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Modeling vegetation density with remote sensing, normalized difference vegetation index and biodiversity plants in watershed area by R.Z. Ekaputri, T. Hidayat, H.K. Surtikanti, W. Surakusumah

“…The study findings indicate that 29 percent of the watershed area exhibits moderately steep topography with a dendritic flow pattern. The assessment of the normalized difference vegetation index demonstrates that the watershed is comprised of multiple sections abundant in high-density vegetation, primarily dedicated to plantations. …”
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Prevalence, risk factors, and prognostic implications of intraoperative bleeding during CF-LVAD implant by Ibrahim Mortada, MD, Christos Kourek, MD, PhD, Rupesh Kshetri, MD, Arun Singhal, MD, Anthony Panos, MD, Alexandros Briasoulis, MD, PhD, Mohammed Mhanna, MD, MPH, Shareef Mansour, MD, Kristine Yumul, MD, Paulino Alvarez, MD, Ernesto Ruiz Duque, MD

“…Background: The use of continuous flow left ventricular assist device (CF-LVAD) has revolutionized the management of advanced heart failure. …”
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Second-Generation Triple Reporter for Bioluminescence, Micro–Positron Emission Tomography, and Fluorescence Imaging by Aparna H. Kesarwala, Julie L. Prior, Jinwu Sun, Scott E. Harpstrite, Vijay Sharma, David Piwnica-Worms

“…Furthermore, a triple-reporter construct was developed in which monster green fluorescent protein (MGFP), a recently available enhanced fluorescent protein, was introduced into the fusion vector downstream of an internal ribosome entry site (IRES) to allow analysis by fluorescence microscopy or flow cytometry without compromising the specific activities of the upstream fusion components. …”
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The Identification and Reservoir Architecture Characterization of Wandering Braided River in Nanpu 1-29 Area, Bohai Bay Basin by Xueqian Pang, Cheng Xue, Yangping Liu, Li Yin, Yufeng Gao, Yanshu Yin

“…The length of the single braided flow belts was 365.16-1349.72 m, and the width was 270.57-1160.54 m. …”
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GPR55 in the tumor microenvironment of pancreatic cancer controls tumorigenesis by Dušica Ristić, Thomas Bärnthaler, Eva Gruden, Melanie Kienzl, Laura Danner, Karolina Herceg, Arailym Sarsembayeva, Julia Kargl, Rudolf Schicho

“…To this end, a KPCY tumor cell line or a GPR55-overexpressing KPCY cell line (KPCY55) from murine PDAC were subcutaneously injected into wildtype (WT) and GPR55 knockout (KO) mice, and immune cell populations were evaluated by flow cytometry. ResultsDeficiency of GPR55 in the TME led to reduced tumor weight and volume, and altered the immune cell composition of tumors, favoring an anti-tumorigenic environment by increasing the number of CD3+ T cells, particularly CD8+ T cells, and the expression of PDL1 on macrophages. …”
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Nutrient inputs shape ecosystem functioning gradients along the pristine, upper Neretva River, Bosnia and Herzegovina by Rubén Del Campo, Barbara Jechsmayr, Veronika Settles, Melanie Ströder, Gabriel Singer

“…Here, we investigated how ecosystem functions and their diversity (estimated as multifunctionality) change along the continuum of the karstic, free-flowing Neretva River in Bosnia and Herzegovina. For this purpose, we measured a subset of fundamental ecosystem functions (ecosystem gross primary production, biofilm net primary production and enzymatic activities, organic matter decomposition) in 11 river reaches from the Neretva headwaters to river sections upstream of the Jablanica reservoir. …”
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B Lymphocyte—A Prognostic Indicator in Post‐Acute Pancreatitis Diabetes Mellitus by Xiaoyan Lin, Xiaoling Li, Junsheng Wang, Huiheng Liu

“…The markers of granulocytes and monocytes and lymphocyte subsets were detected by flow cytometry, and the fluorescence intensity, absolute count and percentage were obtained. …”
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BRAF regulates circPSD3/miR-526b/RAP2A axis to hinder papillary thyroid carcinoma progression by Chuang Li, Xiaojuan Zhao, Jingge Zhao, Jing Zhao, Lemei An, Gang Wu

“…Cell apoptosis was quantified using flow cytometry. The migratory and invasive capabilities of the cells were evaluated via wound healing and transwell assays. …”
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The international trial of nasal oxygen therapy after cardiac surgery (NOTACS) in patients at high risk of postoperative pulmonary complications: Economic evaluation protocol and a... by Siddesh Shetty, Melissa Duckworth, Richard Norman, Jacquita Affandi, Sarah Dawson, Julia Fox-Rushby, NOTACS investigators, NOTACS Trial Steering Committee, NOTACS DMEC Committee

“…<h4>Introduction</h4>High-Flow Nasal Therapy (HFNT) is an innovative non-invasive form of respiratory support. …”
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L-Borneol 7-O-[β-D-Apiofuranosyl-(1→6)]-β-D-Glucopyranoside Alleviates Myocardial Ischemia-Reperfusion Injury in Rats and Hypoxic/Reoxygenated Injured Myocardial Cells via Regulati... by Ziyi Tong, Gaowen Li, Chengxiao Su, Liyan Zhou, Ling Zhang, Qun Chen, Qing Xia

“…Afterward, cell viability, LDH, CD-KM release, apoptosis, and autophagy were evaluated by CCK-8 and ELISA assays, flow cytometry, TUNEL, and immunofluorescence staining, respectively. …”
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Enhancement of systemic and lung-localized CD4⁺ T-cell immune responses by truncation of NS1 protein of a seasonal live influenza vaccine strain by Polina I. Prokopenko, Ekaterina A. Stepanova, Victoria A. Matyushenko, Alexandra Ya. Rak, Anna K. Chistyakova, Arina D. Kostromitina, Tatyana S. Kotomina, Igor V. Kudryavtsev, Artem A. Rubinstein, Alexey S. Komlev, Larisa G. Rudenko, Irina N. Isakova-Sivak

“…Levels of systemic and tissue-resident cytokine-producing CD4+ and CD8+ memory T cells were assessed by intracellular cytokine staining assay with flow cytometry. Replication of engineered vaccine strains in in vitro and in vivo systems was also evaluated. …”
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