Triptolide Inhibits Th17 Response by Upregulating microRNA-204-5p and Suppressing STAT3 Phosphorylation in Psoriasis by Qi He, Xingyue Wu, Quan Shi

“…Hematoxylin and eosin staining was used for the visualization of epidermal thickness. Flow cytometry analysis was employed for examining T helper (Th) 17 cells. …”
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Evaluation of the effect of 48 weeks of BIC/F/TAF and DRV/c/F/TAF on platelet function in the context of rapid ART start by Akif A. Khawaja, Gary Whitlock, Sarah Fidler, Alfredo Soler-Carracedo, Merle Henderson, Graham P. Taylor, Marta Boffito, Michael Emerson

“…Platelet activation was assessed by real-time flow cytometry to examine integrin activation and granule release and platelet aggregation was evaluated by light transmission aggregometry. …”
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Tumor CEMIP drives immune evasion of colorectal cancer via MHC-I internalization and degradation by Ying Zhu, Jiao Li, Tao Zhang, Lei Zhao, Min Jin, Jinghua Ren, Jing Tang, Haihong Wang, Zhenyu Lin, Qing Liang, Ruiqi Li, Biying Zhang, Qingling Hua, Guojie Xu, Jiayuan Chen, Lanqing Wang, Dejun Zhang, Dandan Yu

“…Here, in this research, we aim to address the role of CEMIP in mediating MHC-I expression in tumor cells and investigate the underlying regulatory mechanisms.Method Protein levels were analyzed by western blotting. Flow cytometry analysis was used to examine immune cells. …”
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Database and geospatial mapping study of those eligible for extracorporeal cardiopulmonary resuscitation in the Thames Valley Region, England by Oscar Millerchip, Jasper Eddison, Alex Rosenberg, Jon Bailey, James Raitt

“…Extracorporeal-cardiopulmonary resuscitation (ECPR) is a therapy for refractory out-of-hospital cardiac arrest that can improve survivability by decreasing the time a patient is without adequate perfusion, the low-flow time. Access to ECPR is limited by the number, location and delivery approach of centres offering this therapy. …”
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Functional MRI study of neurovascular coupling in patients with non-lesional epilepsy by Zhisen Li, Xiaoxia Hou, Yanli Lu, Huimin Zhao, Meixia Wang, Qian Gui, Guanhui Wu, Qinrong Xu, Xiaofeng Dong, Qingzhang Cheng, Xiaowen Xu, Hongxuan Feng

“…The neuronal activity and functional connectivity of NLE patients are significantly abnormal, and the neuronal activity of epilepsy patients is closely related to cerebral blood flow (CBF). Neurovascular coupling (NVC) offers insights into the relationship between neuronal activity and CBF. …”
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Human Umbilical Cord Perivascular Cells Prevent Tumor Growth in a Melanoma Tumor-Bearing Mouse Model and Modulate Breast Cancer and Melanoma Cells in a Cell Line-Dependent Manner I... by Lianet Lopez, Hannah Shuster-Hyman, Eden Marco, Hasna Khan, Avishai Gasner, Aleksandra Uzelac, Brandon Wyse, Poonam Mander, Mugundhine Sangaralingam, Joseph Fish, Ariel Gorodensky, Samar Mouazz, Amanda Kauffman, Denis Gallagher, Andrée Gauthier-Fisher, Clifford L. Librach

“…Cell fate and tumor cell proliferation, apoptosis, vascularization as well as tumor-associated immune cells were assessed using immunostaining and flow cytometry. Serum tumor necrosis factor alpha and C-reactive protein levels were measured using enzyme-linked immunosorbent assays. …”
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Plastome characterization and its phylogenetic implications on Lithocarpus (Fagaceae) by Lifang Yang, Shoujun Zhang, Chunya Wu, Xiaolong Jiang, Min Deng

“…The plastome-based phylogeny confirmed the monophyletic status of Lithocarpus and revealed a phylogeographic pattern indicating limited seed-mediated gene flow in the ancestral lineage. The prevalence of cytonuclear discordance in Lithocarpus and other Fagaceae genera suggests that ancient introgression, incomplete lineage sorting, and asymmetrical seed- and pollen-mediated geneflow might contribute to this discordance. …”
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Automated Decision Technique for the Crowd Estimation Method Using Thermal Videos by Nermin K. Negied, Ayman El-Sayed, Asmaa S. Hassaan

“…Counting and detecting the pedestrians is an important and critical aspect for several applications such as estimation of crowd density, organization of events, individual’s flow control, and surveillance systems to prevent the difficulties and overcrowding in a huge gathering of pedestrians such as the Hajj occasion, which is the annual event for Muslims with the growing number of pilgrims every year. …”
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Inhibition of the Ubiquitin-Activating Enzyme UBA1 Suppresses Diet-Induced Atherosclerosis in Apolipoprotein E-Knockout Mice by Jiawei Liao, Xiaolei Yang, Qiuyue Lin, Shuang Liu, Yunpeng Xie, Yunlong Xia, Hui-Hua Li

“…PYR-41 did not alter circulating immune cells determined by flow cytometry but significantly reduced aortic mRNA levels of cytokines related to monocyte recruitment (Mcp-1, Vcam-1, and Icam-1) and macrophage proinflammatory responses (Il-1β and Il-6). …”
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Immune-related gene SOX10 affects ferroptosis in pancreatic cancer and facilitates tumor progression by targeting CMTM7-mediated Wnt/β-catenin signaling pathway by Guixing Jiang, Bicheng Wu, Kaikai Wang, Xiaofan Pu, Senhao Zhou, Xin Zhong, Xiaolong Liu, Suihan Wang, Tianyu Lin

“…To assess cell functions, cell counting kit-8, flow cytometry, scratch test, and Transwell assays were performed. …”
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Biliary atresia: The profile, management and outcome of patients treated at a tertiary hospital in central South Africa by E Brits, S M le Grange

“…Late presentation, cholangitis and cessation of bile flow after an initial successful KPE, and socioeconomic challenges are issues of concern and had a detrimental influence on the outcome of BA in our study population. …”
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PABPN1 functions as a downstream gene of CREB to inhibit the proliferation of preadipocytes by Xiao-Han Zhang, Jia-Xin Li, Xiao-Xu Wu, Qian Zhang, Ming Tian, Si-Qi Yang, Di Liu, Xiu-Qin Yang

“…Preadipocyte proliferation was measured with cell counting kit-8, 5-ethynyl-2′-deoxyuridine, real-time quantitative polymerase chain reaction, Western blotting, and flow cytometry analyses. Additionally, the transcriptional regulation of CREB on PABPN1 were analyzed with dual-luciferase reporter gene and electrophoretic mobility shift assay. …”
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Converting Organic Municipal Solid Waste Into Volatile Fatty Acids and Biogas: Experimental Pilot and Batch Studies With Statistical Analysis by Hojjat Borhany

“…ObjectiveThis research compares a novel line, consisting of pretreatment, acidogenic fermentation, and anaerobic digestion, with single-step anaerobic digestion regarding financial profit and surplus energy. Therefore, a mass flow model was created and refined based on the outputs from the experimental and numerical studies. …”
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Strontium nitrate-dopped zinc oxide-loaded alginate gels with gentamicin for improved wound healing by Suhair Hikmat, Ola Tarawneh, Lama Hamadneh, Rania Hamed, Ala A. Alhusban, Mohammad Hailat, Hadeel Abu Mahfouz, Sawsan Shraim, Alghadeer Al-Shammari, Aya Aljariri, Rafa Abu Rayya, Lana Hamdan

“…Rheology was used to confirm the gel’s preparation, where the samples’ viscosity curves show decreased viscosity with increased shear rate, indicating pseudoplastic flow. The linear viscoelastic region shows constant G’ and G” within the sample structure. …”
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Thermal ablation enhances immunotherapeutic effect of IP-001 on orthotopic liver cancer in a rat model by Yan Li, Samuel S.K. Lam, Chun Fung Wong, Tomas Hode, David Anderson, Robert C.G. Martin

“…Significantly increased infiltration of inflammatory/immune cells were found in the tumor adjacent tissues of MWA+IP-001 mice, compared to the other 3 groups. Flow cytometry results indicated that there were significant increases of cytotoxic T cells, macrophages, dendritic cells and NK cell in the combination of MWA and IP001 treated mice, compared to other 3 groups (p < 0.01). …”
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Clofarabine induces tumor cell apoptosis, GSDME-related pyroptosis, and CD8+ T-cell antitumor activity via the non-canonical P53/STING pathway by Jing Chen, Jie Wu, Xiang Chen, Qian Tao, Nian Liu, Cong Peng, Can Lu, Qiuqiu Li

“…The molecular mechanisms of Clo-induced tumor cell death were analyzed using western blotting, ELISA, reverse transcription-PCR, immunofluorescence, co-immunoprecipitation (CO-IP), short hairpin RNA, co-culture, chromatin immunoprecipitation, and flow cytometry. Clinical data sets were used to analyze the correlation between stimulator of interferon genes (STING)-NFκB signaling and immune infiltration.Results In this study, Clo significantly reduced the growth of melanoma and lung cancer cells. …”
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Assessing Hydropower Potential in Nepal’s Sunkoshi River Basin: An Integrated GIS and SWAT Hydrological Modeling Approach by Rinki Bhattarai, Binaya Kumar Mishra, Deepa Bhattarai, Dipendra Khatiwada, Pankaj Kumar, Gowhar Meraj

“…The hydropower potential at each identified site was evaluated using estimated stream flow and topographical head at various probability of exceedance (PoE) levels (40%, 45%, 50%, and 60%). …”
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Diagnostic Accuracy of Physical Examination Tests for Suspected Acute Anterior Cruciate Ligament Injury: A Systematic Review and Meta-Analysis by Shigeharu Tanaka, Yu Inoue, Yuki Masuda, Hui Tian, Hungu Jung, Ryo Tanaka

“…The methodological quality of the included studies was mostly favorable. For the domain of flow and timing in the QUADAS-2, three studies did not assess the timing between the reference and index tests. …”
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Failure Modes and Fault Morphology by Adam J. Cawood, David A. Ferrill, Kevin J. Smart

“…Distinct failure behaviors are consistent with patchworks of volume neutral, volume gain, and volume loss zones along the fault surfaces, indicating that the characterized faults likely represent dual conduit-seal systems for fluid flow.…”
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Optimal sizing and performance assessment of stand-alone PV systems using optimum hybrid sizing strategy by Mohamad Abed, Amarendra Reddy B, T.R. Jyothsna, Nabil Mohammed

“…Results confirm that the designed SAPVS meets technical requirements, with steady-state and dynamic performances assessed through graphical analyses of electrical characteristics, power flow, and output curves.…”
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