Periodontitis promotes tumor growth and immune evasion via PD-1/PD-L1 by Suli Wang, Fujiao Nie, Qiuyue Yin, Haoyang Tian, Pizhang Gong, Jinhong Ju, Jiayi Liu, Pishan Yang, Chengzhe Yang

“…Tumor volume and weight were recorded and the levels of relevant immune-suppressive cells and T cells were detected by flow cytometry or immunofluorescence. THP-1 cells were stimulated using conditioned media of LPS-stimulated Cal-27 cells and PD-L1 expression was measured by quantitative real-time PCR, western blotting and immunofluorescence. …”
Get full text

A systematic review of the transformation of Lake Beseka from brine to nearly fresh lake water by Yosef Abebe, Taye Alemayehu, Bizuneh Asfaw Abebe, Tena Alamirew, Beteley Teckola, Esayas Alemayehu

“…It is crucial to take into account options to lessen the effects of the adjacent Awash River by upgrading infrastructure and changing the lake's natural flow in order to address the issues with average water quantity. …”
Get full text

Application of the OMERACT Grey-scale Ultrasound Scoring System for salivary glands in a single-centre cohort of patients with suspected Sjögren’s syndrome by Lene Terslev, Simon Krabbe, Viktoria Fana, Uffe M Dohn

“…All underwent grey-scale ultrasound of the parotid and submandibular glands prior to clinical examination, Schirmer’s test, unstimulated salivary flow, blood samples including autoantibody analysis. …”
Get full text

Grouting Design of Rich Water Tunnels and the Calculation of Distance between Annular Blind Pipes by Helin Fu, Pengtao An, Kai Li, Guowen Cheng, Jie Li, Xiaohui Yu

“…On the other hand, the stratum grouting is used to control the discharge flow of groundwater. In the drainage system, it is important to determine the distance between the annular blind pipes, but there is no clear calculation formula, which leads to the designer often relying on experience. …”
Get full text

Long non-coding RNA NEAT1 promotes ovarian granulosa cell proliferation and cell cycle progression via the miR-29a-3p/IGF1 axis by Lina He, Jie Lin, Zhengwen Qin, Qing Xu, Li Hao, Yanhong Fu, Xu Ran, Wei Chen

“…The effects of NEAT1 overexpression or silencing on cell proliferation and cell cycle were evaluated using CCK-8 assays and flow cytometry. The interaction between NEAT1, miR-29a-3p, and IGF1 was examined using dual-luciferase reporter assays, qRT-PCR, and Western blot analysis. …”
Get full text

Understanding the Activation of Platelets in Diabetes and Its Modulation by Allyl Methyl Sulfide, an Active Metabolite of Garlic by Navya Malladi, Ebin Johny, Shravan K. Uppulapu, Vikas Tiwari, Md Jahangir Alam, Ramu Adela, Sanjay K. Banerjee

“…We have evaluated the effect of allyl methyl sulfide on platelet activation by administrating AMS to diabetic rats for 10 weeks. Flow cytometry-based analysis was used to evaluate the platelet activation, platelet aggregation, platelet macrophage interaction, and endogenous ROS generation in the platelets obtained from control, diabetes, and AMS- and aspirin-treated diabetic rats. …”
Get full text

Sex Differences in the Renal Vascular Responses of AT1 and Mas Receptors in Two-Kidney-One-Clip Hypertension by Zahra Pezeshki, Mehdi Nematbakhsh

“…Responses of mean arterial pressure (MAP), renal blood flow (RBF), and renal vascular resistance (RVR) to Ang II were determined. …”
Get full text

Sporoderm-Removed Spore Powder May Suppress the Proliferation, Migration, and Invasion of Esophageal Squamous Cell Carcinoma Cells Through PI3K/AKT/mTOR and Erk Pathway by Jingyan Liu Master degree, Tao Zeng Doctor degree

“…Apoptosis and the cell cycle were analyzed by flow cytometry. Wound healing and Transwell assays were used to analyze the migration of KYSE140 and KYSE510 cells. …”
Get full text

Teaching System Design of Art Design Major under the Background of Economic Management Mode Change by Jiaxin Zheng

“…These methods include the use case diagram of the subsystem, the flow chart of the main module, the system architecture, and the design and application of UML. …”
Get full text

Needle tracking and segmentation in breast ultrasound imaging based on spatio-temporal memory network by Qiyun Zhang, Jiawei Chen, Jinhong Wang, Haolin Wang, Yi He, Yi He, Bin Li, Zhemin Zhuang, Huancheng Zeng

“…The proposed network integrates a hybrid encoder that employs CNN-Transformer architectures, along with an optical flow estimation method. From the Ultrasound Imaging Department at the First Affiliated Hospital of Shantou University, we developed a real-time segmentation dataset specifically designed for ultrasound-guided needle puncture procedures in breast tumors, which includes ultrasound biopsy video data collected from 11 patients.ResultsExperimental results demonstrate that this model significantly outperforms existing methods in improving the positioning accuracy of needle and enhancing the tracking stability. …”
Get full text

Integrating whole-genome re-sequencing and transcriptome data to reveal the molecular mechanism of TBX5 gene regulating feathered feet in Guangxi native chickens by Zhuliang Yang, Yongcui Liu, Jintang Luo, Cong Xiao, Hong Yuan, Wenya Du, Xiurong Yang

“…EdU fluorescence staining, flow cytometry, and migration assays demonstrated that moderate expression of TBX5 can promote the proliferation and migration of dermal fibroblasts, while TBX5 interference produced opposite results. …”
Get full text

A Novel Traffic Analysis Zone Division Methodology Based on Individual Travel Data by Kai Du, Jingni Song, Dan Chen, Ming Li, Yadi Zhu

“…Urban rail transit passenger flow forecasting often relies on the traditional “four-step” method, where the division of traffic analysis zones (TAZs) is critical to ensuring prediction accuracy. …”
Get full text

Complications of Estimating Hatchery Introgression in the Face of Rapid Divergence: A Case Study in Brook Trout (Salvelinus fontinalis) by Bradley Erdman, Wesley Larson, Matthew G. Mitro, Joanna D. T. Griffin, David Rowe, Justin Haglund, Kirk Olson, Michael T. Kinnison

“…The results from these analyses suggest that wild populations in this region are genetically distinct even at small spatial scales, lack strong hydrologically associated population structure, rarely exchange gene flow, and have small effective population sizes. …”
Get full text

The Influence of Direct Reduced Iron Size on EAF Smelting by Kang　Xu, Cao　Zhenwei, Wang　Qiang, Liu　Chong, Li　Shuangjiang, Zhang　Junfen

“…The small-sized DRI retained in some slag and the small steel droplets formed after melting of small-sized DRI flow out with the overflow slag at the furnace door. …”
Get full text

Dual-functional passivation on highly-efficient air-processed FAPbI₃ perovskite solar cells fabricated under high humidity without auxiliary equipment by Bo-Tau Liu, His-Sheng Su, I-Ru Chen, Rong-Ho Lee, Yi-Fang Su, Kai-Ting Sun, Shoaib Siddique

“…While numerous methods have been proposed to address this issue, most efforts have relied on glove-box conditions, substrate heating, or air-knife flow. To date, few studies have reported a strategy for fabricating highly efficient FAPbI3 perovskite solar cells (PSCs) under humid conditions. …”
Get full text

The Construction of Identities in Space Inversion (the Case of Blagoveshensk and Chei-Che) by I. Y. Okunev, D. V. Basova, M. I. Tislenko

“…It was an attempt to define the mechanisms of creating space identity at the flexible border marked by fluctuations of economic, political and cultural flows. There is a case of the Chinese town Chei-Che which is more open towards a Russian influence as well as to a deeper integration with the Russian side. …”
Get full text

Tasquinimod suppresses tumor cell growth and bone resorption by targeting immunosuppressive myeloid cells and inhibiting c-MYC expression in multiple myeloma by Marie Törngren, Rong Fan, Eline Menu, Helena Eriksson, Karin Vanderkerken, Andrew Chantry, Hatice Satilmis, Niels Vandewalle, Emma Verheye, Philip Vlummens, Anke Maes, Catharina Muylaert, Elke De Bruyne, Holly Evans, Nathan De Beule, Dirk Hose, Ken Maes, Karine Breckpot, Kim De Veirman

“…We aimed to gain more insights into its mechanisms of action both on the myeloma cells and the immune microenvironment.Methods We analyzed the effects of tasquinimod on MM cell viability, cell proliferation and downstream signaling pathways in vitro using RNA sequencing, real-time PCR, western blot analysis and multiparameter flow cytometry. Myeloid cells and T cells were cocultured at different ratios to assess tasquinimod-mediated immunomodulatory effects. …”
Get full text

Long-Term and Short-Term Effects of Hemodialysis on Liver Function Evaluated Using the Galactose Single-Point Test by Yi-Chou Hou, Wen-Chih Liu, Min-Tser Liao, Kuo-Cheng Lu, Lan Lo, Heng-Chih Pan, Chia-Chao Wu, Oliver Yoa-Pu Hu, Hung-Shang Tang

“…Since the metabolism of galactose is dependent on liver blood flow and hepatic functional mass, further studies are needed.…”
Get full text

Improvement of Microstructure and Properties of Q235 Steel by Iron-Based Laser Cladding Coating by Yuanwei He, Yu Gu, Li Tang, Hu Wang

“…Three types of specimens were obtained from Q235 base material using a 5 kW cross-flow CO2 laser beam. Sample 1 and sample 2 were obtained by the addition of rosin to the iron-based alloy powder. …”
Get full text

Defining indicators for disease burden, health outcomes, policies and barriers and facilitators to health services for migrant populations in the Middle East and North African regi... by Charles Agyemang, Ali Ardalan, Farah Seedat, Bouchra Assarag, Nuria Casamitjana, Kolitha Wickramage, Sally Hargreaves, Ana Requena-Mendez, Fouad M Fouad, Dominik Zenner, Anna Deal, Stella Evangelidou, Wafa Saidi, Chiaki Ito, Oumnia Bouaddi, Moudrick Abdellatifi, Alba Cuxart-Graell, Hassan Edries, Eman Elafef, Taha Maatoug, Anissa Ouahchi, Liv Mathilde Pampiri, Sara Arias, Adel Abdelkhalek, Ahmed Hamed Arisha, Ibrahim Ahmed Bani, Aasmaa Chaoui, Wafa Chemao-Elfihri, Kenza Hassouni, Mahmoud Hilali, Mohamed Khalis, Wejdene Mansour, Ali Mtiraoui, MENA Migrant Health Working Group, Asad Adam, Adnene Ben Haj Aissa, Salma Altyib, Hanen Ben Belgacem, Imane Belkhammar, Thomas Calvot, Luciana Ceretti, Nelly Chavassieux, Hassan Chrifi, Mohamed Douagi, Algdail Elnil, Gonzalo Fanjul, Ahmed Hamed, Abdedayem Khelifi, Lora Makhlouf, Maissa Mokni, Davide Olchini, Tarik Oufkir, Nasong Park, Giuseppe Raffa, Sandra Santafé, Alice Sironi, Fatma Temimi, Zeineb Turki

“…Introduction The Middle East and North African (MENA) region is characterised by high and complex migration flows, yet little is known about the health of migrant populations, their levels of underimmunisation and access to healthcare provision. …”
Get full text