IL-1β Pretreatment Improves the Efficacy of Mesenchymal Stem Cells on Acute Liver Failure by Enhancing CXCR4 Expression by He Nie, Fangmei An, Jie Mei, Cheng Yang, Qiang Zhan, Qinglin Zhang

“…Then, Western blot was performed to detect the c-Met and CXCR4 expression of MSCs and Pre-MSCs and followed by flow cytometry to detect the meaningful indicators. …”
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Influence of tunnel construction on karst groundwater environment in central Huaying Mountain by Sheng CHENG, Mo XU, Qiang XIA

“…The results show that human activities, particularly underground engineering, have altered the hydrology of the central Huaying Mountain area, transforming multiple spring points into reduced or disconnected subsurface river flows, especially in the Tianchi synclinal karst valley and Green Water Cave. …”
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An artificial insurance framework for a hydrogen-based microgrid to detect the advanced cyberattack model by Mahan Fakhrooeian, Ali Basem, Mohammad Mahdi Gholami, Nahal Iliaee, Alireza Mohammadi Amidi, Amin Heydarian Hamzehkanloo, Akbar Karimipouya

“…With this in mind, this paper explores a novel advanced attack model named the false transferred data injection (FTDI) attack aiming to manipulatively alter the power flowing from the microgrid to the upstream grid to raise voltage usability probability. …”
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Unleashing the potential of a low CpG Passer transposon for superior CAR-T cell therapy by Jianyao Zeng, Yan Sun, Yan Sun, Yuan Fang, Xiaodie Wang, Qian Huang, Pingjing Zhang, Meiqi Shao, Pei Wang, Jingbo Cheng, Meng Di, Tao Liu, Qijun Qian, Qijun Qian, Qijun Qian

“…Additionally, de novo DNA methylation programs promote T cell exhaustion, which poses a significant limitation for CAR-T cell therapy applications.MethodsHigh-throughput liquid protein chip and CBA analyses were utilized to determine the expression levels of inflammatory factors. Flow cytometry and luciferase reporter assays were employed for mutation screening. …”
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Encrypted traffic classification method based on convolutional neural network by Rongna XIE, Zhuhong MA, Zongyu LI, Ye TIAN

“…Aiming at the problems of low accuracy, weak generality, and easy privacy violation of traditional encrypted network traffic classification methods, an encrypted traffic classification method based on convolutional neural network was proposed, which avoided relying on original traffic data and prevented overfitting of specific byte structure of the application.According to the data packet size and arrival time information of network traffic, a method to convert the original traffic into a two-dimensional picture was designed.Each cell in the histogram represented the number of packets with corresponding size that arrive at the corresponding time interval, avoiding reliance on packet payloads and privacy violations.The LeNet-5 convolutional neural network model was optimized to improve the classification accuracy.The inception module was embedded for multi-dimensional feature extraction and feature fusion.And the 1*1 convolution was used to control the feature dimension of the output.Besides, the average pooling layer and the convolutional layer were used to replace the fully connected layer to increase the calculation speed and avoid overfitting.The sliding window method was used in the object detection task, and each network unidirectional flow was divided into equal-sized blocks, ensuring that the blocks in the training set and the blocks in the test set in a single session do not overlap and expanding the dataset samples.The classification experiment results on the ISCX dataset show that for the application traffic classification task, the average accuracy rate reaches more than 95%.The comparative experimental results show that the traditional classification method has a significant decrease in accuracy or even fails when the types of training set and test set are different.However, the accuracy rate of the proposed method still reaches 89.2%, which proves that the method is universally suitable for encrypted traffic and non-encrypted traffic.All experiments are based on imbalanced datasets, and the experimental results may be further improved if balanced processing is performed.…”
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Reverse-Phase Chromatographic Determination and Intrinsic Stability Behavior of 5-[(4-Chlorophenoxy)Methyl]-1,3,4-Oxadiazole-2-Thiol by Shehzadi Naureen, Hussain Khalid, Islam Muhammad, Bukhari Nadeem Irfan, Khan Muhammad Tanveer, Salman Muhammad, Siddiqui Sabahat Zahra, Rehman Aziz-Ur, Abbasi Muhammad Athar

“…Isocratic elution was carried out at a flow rate of 1.0 mL min-1 through a Promosil C18 column maintained at 25 °C, using the mobile phase comprising acetonitrile and aqueous o-H3PO4 (pH 2.67) (1:1, V/V). …”
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Mucosal Immunization with an Influenza Vector Carrying SARS-CoV-2 N Protein Protects Naïve Mice and Prevents Disease Enhancement in Seropositive Th2-Prone Mice by Mariia V. Sergeeva, Kirill Vasilev, Ekaterina Romanovskaya-Romanko, Nikita Yolshin, Anastasia Pulkina, Daria Shamakova, Anna-Polina Shurygina, Arman Muzhikyan, Dmitry Lioznov, Marina Stukova

“…Immune response in immunized and challenged mice was analyzed through serological methods and flow cytometry. <b>Results</b>: Double intranasal immunization of naïve mice with FluVec-N reduced weight loss and viral load in the lungs following infection with the SARS-CoV-2 beta variant. …”
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Ex Vivo Evaluation of Secretion-Clearing Device in Reducing Airway Resistance within Endotracheal Tubes by Christopher Waters, R. Constance Wiener, Hamed M. Motlagh

“…The pressure drop through the ETTs was measured at a flow rate of 60 L/min before and after cleaning with the ETT-CD and compared with unused, similarly sized controls tubes. …”
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Insulin-Attenuated Inflammatory Response of Dendritic Cells in Diabetes by Regulating RAGE-PKCβ1-IRS1-NF-κB Signal Pathway: A Study on the Anti-Inflammatory Mechanism of Insulin in... by Liding Zhao, Ya Li, Qingbo Lv, Min Wang, Yi Luan, Jiale Song, Guosheng Fu, Junbo Ge, Yunzeng Zou, Wenbin Zhang

“…CD83, CD86, and type II major histocompatibility complex (MHC-II) of BMDCs were measured by flow cytometry. The fluctuations in the RNA levels of cytokines and chemokines were analyzed by quantitative RT-PCR. …”
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Study of Transition Region of p-Type SiOx:H as Window Layer in a-Si:H/a-Si1-yGey:H Multijunction Solar Cells by Pei-Ling Chen, Po-Wei Chen, Chuang-Chuang Tsai

“…By increasing the H2-to-SiH4 flow ratio (RH2) from 10 to 167, the SiOx:H(p) films remained amorphous and exhibited an increased hydrogen content from 10.2% to 12.2%. …”
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Operational Evaluation of Part-Time Shoulder Use for Interstate 476 in the State of Pennsylvania by Sean Coffey, Seri Park

“…I-476 is a major commuting route for the region, and the additional capacity during the commuting periods would greatly improve the flow of the surrounding network. This analysis was completed using a Vissim model simulating the 7 AM to 9 AM commuting period. …”
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The role of natural exosomes from SHED-MSC in immunoregulation of M0/M1 polarized macrophage cells by Ali Fallah, Abasalt Hosseinzadeh Colagar, Ayyoob Khosravi, Ayyoob Khosravi, Azadeh Mohammad-Hasani, Azadeh Mohammad-Hasani, Mohsen Saeidi, Mohsen Saeidi

“…This study focused on exosomes derived from stem cells of human exfoliated deciduous teeth (SHED-MSC-EXOs) and their role in managing the balance of immunoregulatory macromolecules that play a role in the underlying immunoregulatory mechanisms in THP-1-derived M0/M1 macrophage cells.MethodsFlow cytometry confirmed the expression of CD14, CD68, CD80, and CD86 markers in these macrophages. …”
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Design and fabrication of a device for cleaning greenhouse roofs by Ahmed Amin, Xiaochan Wang, Zhao Lianyuan, Yinyan Shi, Ren Xiaoyan, Mahmoud Okasha, Reda Hassanien Emam Hassanien

“…Meanwhile, it could control the water pressure and water flow rate. The analysis of the intensity disorder and the dissipation rate showed positive results for the system and valuable insights for efficient and safe system design. …”
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A Study on the Induction of Multi-Type Immune Responses in Mice via an mRNA Vaccine Based on Hemagglutinin and Neuraminidase Antigen by Mengyuan Liu, Yixuan Liu, Shaohui Song, Qiurong Qiao, Jing Liu, Yun Xie, Jian Zhou, Guoyang Liao

“…Additionally, the immune responses elicited by the two immunization strategies were characterized using flow cytometry and ELISA. Comparative analyses were performed with mice immunized individually with the IAV HA mRNA vaccine, IAV NA mRNA vaccine, and inactivated vaccine. …”
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Gardnerella vaginalis-binding IgA in the urethra of sexually experienced males by Rachel Liu, R. M. Galiwango, Daniel Park, Sanja Huibner, Maliha Aziz, Aggrey Anok, James Nnamutete, Yahaya Isbirye, John Bosco Wasswa, Deo Male, Godfrey Kigozi, Aaron A. R. Tobian, Jessica L. Prodger, Cindy Liu, Bryan Coburn, Rupert Kaul

“…While Streptococcus mitis is a consistent component of the urethral flora, the presence of Gardnerella vaginalis has been linked with prior penile-vaginal sex and urethral inflammation. Here, we use a flow cytometry-based bacterial assay to quantify urethral IgA and IgG that bind G. vaginalis and S. mitis in a cross-sectional cohort of 45 uncircumcised Ugandan men and to evaluate their association with the urethral microbiome and local soluble immune factors. …”
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Effect of Airflow Temperature on the Formation of Initial Flame Kernel and the Propagation Characteristics of Flame by Jianzhong Li, Jian Chen, Li Yuan, Ge Hu

“…Combining high-speed camera and dynamic temperature acquisitions at the outlet of combustor, the internal triggering mode was used under a constant fuel flow rate and airflow velocity. This combined system simultaneously recorded the formation of initial flame kernel, flame propagation, and outlet temperature variation of combustor under different airflow temperatures. …”
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Correlation of precisely fabricated geometric characteristics of DNA-origami nanostructures with their cellular entry in human lens epithelial cells by Yexuan Guo, Tianze Xiong, Hong Yan, Rui Xue Zhang

“…Atomic force microscopy and agarose gel electrophoresis showed that four DONs self-assembled within 3.5h with up to 59% yield and exhibited structural intactness in cell culture medium for 4 h. Flow cytometry analysis of four Cy5-labelled DONs in hLECs HLE-B3 found time-dependent cellular uptake over 2 h, among which the octahedron and triangle had higher cellular accumulation than the rod and ring. …”
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Enhancing Cervical Cancer Classification: Through a Hybrid Deep Learning Approach Integrating DenseNet201 and InceptionV3 by Abhiram Sharma, R. Parvathi

“…The experimental setup utilizes Python, TensorFlow, and Keras within a GPU-enabled environment to handle computational demands effectively. …”
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Enucleated bone marrow-derived mesenchymal stromal cells regulate immune microenvironment and promote testosterone production through efferocytosis by Lu Sun, Jiayu Huang, Xuezi Wang, Peng Huang, Baolin Dong, Zehang Liang, Jiahong Wu, Jiancheng Wang

“…The organelles and cellular functions of enucleated BMSCs were analyzed by immunofluorescence staining and flow cytometry. Extracellular vesicles (EVs) were isolated by ultracentrifugation and characterized. …”
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DKK3 promotes renal fibrosis by increasing MFF-mediated mitochondrial dysfunction in Wnt/β-catenin pathway-dependent manner by Jianling Song, Yanxia Chen, Yan Chen, Minzi Qiu, Wenliu Xiang, Ben Ke, Xiangdong Fang

“…Oxidative stress and mitochondria function were also evaluated. CCK-8 and flow cytometry were utilized to assess cell viability and apoptosis. …”
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