Development and Validation of an UHPLC-MS/MS Method for Quantification of DMAG in Rat Plasma and Its Application in a Preliminary Pharmacokinetic Study in Thrombocytopenia Rats by Yan Li, Yuqing Wang, Zhiqiang Zhao, Yunxia Li, Cheng Peng, Jianming Wu

“…DMAG and hispidulin (internal standard, IS) were separated on an Acquity Shim-pack GIST column using 0.1% formic acid in water and acetonitrile as the mobile phase with a total run time of 5 min and gradient elution at a flow rate of 0.3 mL/min. The recovery and matrix effects of DMAG were within 94.85%–100.40% and 94.55%–102.46%, respectively. …”
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Mechanism of Action of Decitabine in the Treatment of Acute Myeloid Leukemia by Regulating LINC00599 by Fan Du, Ting Jin, Li Wang

“…For each group, apoptosis and reactive oxygen species (ROS) levels were detected using flow cytometry. Reverse transcription polymerase chain reaction (RT-PCR) was performed to examine the expression of lncRNA LINC00599. …”
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Cancer cells avoid ferroptosis induced by immune cells via fatty acid binding proteins by Maria Angelica Freitas-Cortez, Fatemeh Masrorpour, Hong Jiang, Iqbal Mahmud, Yue Lu, Ailing Huang, Lisa K. Duong, Qi Wang, Tiffany A. Voss, Claudia S. Kettlun Leyton, Bo Wei, Wai-Kin Chan, Kevin Lin, Jie Zhang, Efrosini Tsouko, Shonik Ganjoo, Hampartsoum B. Barsoumian, Thomas S. Riad, Yun Hu, Carola Leuschner, Nahum Puebla-Osorio, Jing Wang, Jian Hu, Michael A. Davies, Vinay K. Puduvalli, Cyrielle Billon, Thomas P. Burris, Philip L. Lorenzi, Boyi Gan, James W. Welsh

“…Methods included mass spectrometry-based lipidomics, targeted lipidomics, Oil Red O staining, Seahorse analysis, quantitative PCR, immunohistochemistry, PPARγ transcription factor assays, ChIP-seq, untargeted lipidomic analysis, ROS assay, ex vivo co-culture of CD8+ T cells with cancer cells, ATAC-seq, RNA-seq, Western blotting, co-immunoprecipitation assay, flow cytometry and Imaging Mass Cytometry. Results PD1-resistant tumors upregulate Fabp7, driving protective metabolic changes that shield cells from ferroptosis and evade anti-tumor immunity. …”
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Experimental study and model prediction of the influence of different factors on the mechanical properties of saline clay by Hui Cheng, Lingkai Zhang, Chong Shi, Pei Pei Fan

“…The boundary point of the 2% salt content divides the effect of salt ions from promoting free water flow to blocking seepage channels, with the proportion of micropores being the primary influencing factor. (4) Employing statistical theory and machine learning algorithms, dry density, water content, and salinity are used to predict mechanical index values. …”
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Environmental Life Cycle Assessment of Healthcare Waste Valorisation Alternatives by Zlaugotne Beate, Zandberga Anda, Gusca Julija, Kalnins Silvija Nora

“…The results of the LCA demonstrate that from the environmental impact perspective, the best scenarios are syngas production (VS4) with cumulative result −11Pt and −237 kg CO2 eq for global warming impact category and RDF (VS3) with result −5Pt and −195 kg CO2 eq for global warming impact category, as the entire iHCW flow is recycled into energy. However, when recycling waste, the most valuable product must be created, and then the best results from plastic recycling are plastic components for hydroponics (VS6) with result 50Pt and 1181 kg CO2 eq for global warming impact category and reinforced asphalt (VS1) with result 53 Pt and 1246 kg CO2 eq for global warming impact category. …”
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Impact of Timing the Combination of Radiotherapy and PD-1 Inhibitors on Outcomes in Patients with Hepatocellular Carcinoma by Zhong L, Peng W, Sun J, Luo Y, Sheng H, Wu Y, Zhou T, Zhou C, Cao C

“…In the mouse model, synchronous treatment significantly inhibited tumor growth compared to sequential treatment (293.4± 45.18 mm3 versus 602.7± 41.68 mm3; P=0.001). Flow cytometry revealed an increased Tregs/CD3+ T cell ratio and a decreased CD8+/Treg cell ratio post-radiotherapy, suggesting an immunosuppressive tumor microenvironment.Conclusion: Synchronous treatment demonstrated superior efficacy in treating HCC compared to sequential treatment, with manageable adverse events. …”
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The Utility of Neutrophil CD64 and Presepsin as Diagnostic, Prognostic, and Monitoring Biomarkers in Neonatal Sepsis by Heba E. Hashem, Rania M. Abdel Halim, Sherin A. El Masry, Amira M. Mokhtar, Noureldin M. Abdelaal

“…Laboratory sepsis evaluation included highly sensitive CRP (hs-CRP), CBC, in addition to nCD64 (flow cytometry technique), and presepsin measurement (CLEIA technique combined with Magtration® technology); the diagnosis was confirmed thereafter by positive blood culture results (BacT/Alert system). …”
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Solid Self-Microemulsifying Drug Delivery System for Improved Oral Bioavailability of Relugolix: Preparation and Evaluation by Li ZL, Deng GX, Fang CZ, Zhao YQ, Yuan J, Chen L, Zhong HJ, Guo F

“…The optimized RLGL-S-SMEDDS were evaluated in terms of particle size, zeta potential, morphology analysis, thermodynamic stability, drug release, flow properties, transporter pathways in Caco-2 cells, the influence of excipients on the intestinal transporters, transport within Caco-2 cell monolayers and transport in lymphocyte. …”
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Harbor water pollution by heavy metal concentrations in sediments by L. Sulistyowati, Y. Yolanda, N. Andareswari

“…Pollution problems increase with the anthropogenic activities around coastal areas, as well as activities in the Belawan and Deli River watersheds, because the pollutant will flow from the upstream to the estuary area.…”
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Remote Continuous Microinjury-Triggered Cytokines Facilitate Severe Diabetic Foot Ulcer Healing via the Ras/Raf/MEK/ERK Pathway by Huang X, Liu J, Wu X, Mo Y, Luo X, Yang Y, Yang C, Liang X, Liang R, Chen Y, Fan Z, Lu W, Chen Y, Hua Q

“…Enzyme-linked immunosorbent assay, flow cytometry, immunohistochemistry, and Western Blot were used to assess angiogenesis and the activity of endothelial progenitor cells (EPCs) and the Ras/Raf/MEK/ERK signaling pathway.Results: We found accelerated wound healing, improved epidermal continuity, and increased dermal thickness in the TTT group than the control and the sham groups. …”
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Pengaruh Pemijatan Perineum pada Primigravida terhadap Kejadian Ruptur Perineum saat Persalinan di Bidan Praktek Mandiri di Kota Bengkulu Tahun 2014 by Wewet Savitri, Ermawati ., Elda Yusefni

“…A method of reduce perineal rupture is a perineal massage to promote health, blood flow and elasticity of the perineum. The objekctive of this study was to determine the effect of perineal massage on the incidence of perineal rupture in primigravid during childbirth in Independent Practice of Midwifery in the city of Bengkulu year 2014. …”
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Progress of research on aviation kerosene preparation using bioethanol by Pan LI, Yu ZHAO, Xingguo WU, Lulu ZHANG, Junhao HU, Wei CHEN, Jing BAI, Chun CHANG, Shuqi FANG

“…Currently, the ATJ process still suffers several disadvantages, such as long process flow and low conversion efficiency. The conversion route from ethanol to jet kerosene is complex and requires three different catalysts. …”
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Danggui-Shaoyao-San Can Ameliorate Alzheimer’s Disease by Inhibiting Hippocampal Neuron Apoptosis: Findings from Serum Pharmacology by Zhang KX, Zhang JW, Jiang YH, Wang YR, Liu ZL, Ding PL, Wang XY, Cui WQ, Xu XQ, Wang YH

“…Enzyme linked immunosorbent assay (Elisa), quantitative polymerase chain reaction (q-PCR), Morris water maze,Western blot (WB), Immunohistochemical and Immunofluorescence were used to study the effect of DSS on AD. Flow cytometry and Cell Counting Kit-8 (CCK-8) reveal the effect of DSS serum on HT-22 proliferation and apoptosis.Results: A total of 57 metabolic components were screened in DSS serum. …”
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Study on the aquaculture capacity of whiteleg shrimp (Litopenaeus vannamei)-razor clam (Sinonovacula constricta) tandem mariculture pond by Chunpu Zhao, Gege Bao, Bocheng Mei, Yunhui Luo, Jilin Xu, Shanliang Xu, Danli Wang

“…Through monthly sampling, we estimated the carrying capacity of Sinonovacula constricta by Parsons T R & Takahashi M Trophic Efficiency Method and Tait coastal waters energy flow analysis model. The carrying capacity of Litopenaeus vannamei was estimated by using models based on non-ionic ammonia emission standard and nitrogen emission standard. …”
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Addition of Vitamin C on Diet to Improve Immunity on Tiger Grouper, Epinephelus fuscoguttatus to Infection Viral Nervous Necrosis by F. Johnny, K. Mahardika, I.N.A. Giri, D. Roza

“…Each tank is equipped with flow-through water system and aeration.  Experimental diets were formulated to have the same nutrients content except of vitamin C stable form (L-ascorbyl-2-Phosphate-Magnesium) with different dose, that is 0; 300; 600; 1.200 mg/kg diet was added to each formula.  …”
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Human GM-CSF/IL-3 enhance tumor immune infiltration in humanized HCC patient-derived xenografts by Kelley Weinfurtner, David Tischfield, George McClung, Jennifer Crainic, John Gordan, Jing Jiao, Emma E. Furth, Wuyan Li, Erena Tuzneen Supan, Gregory J. Nadolski, Stephen J. Hunt, David E. Kaplan, Terence P.F. Gade

“…Tumor, blood, liver, and spleen were analyzed by flow cytometry and immunohistochemistry. Results: Humanized NOG-EXL mice demonstrated earlier and higher levels of human chimerism compared to humanized NOG mice (82.1% vs. 43.8%, p <0.0001) with a greater proportion of human monocytes (3.2% vs. 1.1%, p = 0.001) and neutrophils (0.8% vs. 0.3%, p = 0.02) in circulation. …”
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Treatment of pancreatic cancer with irreversible electroporation and intratumoral CD40 antibody stimulates systemic immune responses that inhibit liver metastasis in an orthotopic... by Partha Ray, Tomoko Hayashi, Karen Messer, Stephen Schoenberger, Aaron Miller, Dennis Carson, Sara McArdle, Zbigniew Mikulski, Jayanth S Shankara Narayanan, Suna Erdem, Herve Tiriac, Minya Pu, Rebekah R White

“…Metastatic disease and immune infiltration in the liver were analyzed 14 days postprocedure using flow cytometry and multiplex immunofluorescence imaging with spatial analysis. …”
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Mitochondrial reactive oxygen species trigger metformin-dependent antitumor immunity via activation of Nrf2/mTORC1/p62 axis in tumor-infiltrating CD8T lymphocytes by Kazuhiro Kakimi, Hirokazu Matsushita, Masaaki Komatsu, Eiryo Kawakami, Mikako Nishida, Nahoko Yamashita, Taisaku Ogawa, Keita Koseki, Eiji Warabi, Tomoyuki Ohue, Katsuyuki Shiroguchi, Heiichiro Udono

“…Expressions of glucose transporter (Glut)-1, mitochondrial reactive oxygen species (mtROS), interferon (IFN)-γ, Ki67, autophagy markers, activation markers for NF-E2-related factor 2 (Nrf2), and mammalian target of rapamaycin complex 1 (mTORC1) in CD8+ tumor-infiltrating T lymphocytes (CD8TILs) were examined by flow cytometry analysis. In addition, conditional knockout mice for Nrf2 and p62 were used to detect these markers, together with the monitoring of in vivo tumor growth. …”
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Reflexive Methods of Knowledge in Educational Activities: Phenomenological Hermeneutics by T. N. Korneenko

“…Today, lifelong learning and self-education individual skills are of paramount importance for each representative of the society in relation to the world trends of a post-industrial era: economic domination in the innovative sector, progressive increase of intensity of information flows and rates of distribution of high technologies, rapid development of science and industry of knowledge, quickly changing and becoming tougher requirements to professionalism, qualification and creativity of experts irrespective of the sphere of employment. …”
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Production of Biogas from Bio-Degradable Municipal Solid Waste – A Way of Resource Recovery. by Atatiru, Faiza

“…During the design, the following results were arrived at considering an average of 5 persons per family each having a solid waste generation rate of 0.2kg/person/day in Uganda: flow rate of 0.003m3, reactor volume of 0.09m3, TS of 0.2kg of dry matter, VS of 1.78kgVs/m3 and OLR of 0.059 kg Vs.…”
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