Dexmedetomidine pretreatment enhances protective effect efficacy of pericytes agaist acute lung injury in septic mice by LUO Xi, ZHANG Zisen, YANG Ao

“…The mice of the PCs and Dex-PCs groups received a tail venous injection of 5×105 PC cells， respectively， whereas those of the Sham and ALI groups received equal volume of normal saline. Flow cytometry， immunofluorescence assay， whole-body volume tracing system and ELISA were used to observe the changes in the colonization of PCs， pulmonary vascular permeability， lung function， serum TNF-α and IL-6 levels， as well as the survival rate and survival time of mice at 72 h after LPS stimulation. …”
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Riesgo de contagio por COVID-19 en un entorno urbano (Málaga, noviembre del 2020) by Ángel Miramontes Carballada, Rubén Lois González, María Jesús Perles Roselló, Jesús Miranda Páez

“…Along with the analysis of the spatial distribution of cases and the presence of patterns and trends, aspects such as the analysis of population flows and movements, clinical, social and epidemiological vulnerability, or the socio-economic repercussions of the crisis have been addressed. …”
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The Managers' Perceptions of Telework Implementation Requirements in the Algerian Companies: A Post Covid-19 Assessment by Riad BENGHEBRID

“…Findings: The results concluded that accelerating the telework implementation in Algerian company requires, firstly, the legislative framework by reviewing the labor law that regulates and documents the existing relationship between the teleworker and the employer, and also consistent with the developments of the current situation, and secondly to, the presence of digital infrastructure that goes beyond the need for designated places to include the presence of a wide-coverage, protected, and high-flow communication network, meaning preparing the primary environment with basic equipment and software, thirdly, the existence of training program in order to help to make the best use of information technology (ICT) and reduces the need for supervision and follow-up. …”
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Knockout of the inhibitory receptor TIGIT enhances the antitumor response of ex vivo expanded NK cells and prevents fratricide with therapeutic Fc-active TIGIT antibodies by Dean A Lee, Jeremiah L Oyer, Md Faqrul Hasan, Tayler J Croom-Perez, Liza D Robles-Carrillo, Sanjana Kumar, Brendan W Andersen, Brian P Tullius, Alicja J Copik, Amanda R Campbell, Thomas A Dieffenthaller, Catherine A Cash, Jonathan E Eloriaga, Meisam Naeimi Kararoudi

“…Phenotypic and transcriptomic properties of TIGIT KO and wild-type (WT) NK cells were compared with flow cytometry, CyTOF, and RNA sequencing. The effect of TIGIT KO on NK-cell cytotoxicity was determined by calcein-AM release and live cell imaging-based cytotoxicity assays. …”
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Inhibition of miR-182-5p Targets FGF9 to Alleviate Osteoarthritis by Yang Sun, Sanmao Su, Mengjun Li, Ang Deng

“…Cell counting kit-8 (CCK8), plate cloning assay, and flow cytometry were conducted to evaluate cell proliferation and apoptosis. …”
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Vinblastine resets tumor-associated macrophages toward M1 phenotype and promotes antitumor immune response by Jing Wang, Jin Wang, Yi-Na Wang, Yuan-Yuan Wang, Wen-Juan Bai, Nai-Jun Miao

“…However, no study to date has explored the effect of VBL on TAM shape changes and its role in tumor immune response.Method We used fluorescent staining of the cytoskeleton and quantitative analysis to reveal the morphological differences between M0, M1, M2, TAM and VBL-treated TAM. Flow cytometry was used to confirm the polarization states of these macrophages using a cell surface marker-based classification. …”
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Intratumoral BO-112 in combination with radiotherapy synergizes to achieve CD8 T-cell-mediated local tumor control by Helena Escuin-Ordinas, Ignacio Melero, Marisol Quintero, Jose Luis Perez-Gracia, Maite Alvarez, Maria E Rodriguez-Ruiz, Miguel F Sanmamed, Paloma Sanchez-Mateos, Inmaculada Rodríguez López, Irantzu Serrano-Mendioroz, Celia Barrio-Alonso, Eneko Garate-Soraluze, Victor Diaz Pascual, Leire Arbea Moreno

“…BO-112 and focal irradiation were used to make the proof-of-concept for local immunotherapy plus radiation therapy combinations.Methods Murine transplantable tumor cell lines (TS/A, MC38 and B16-OVA) were used to show increased immunogenic features under irradiation, as well as in bilateral tumor models in which only one of the lesions was irradiated or/and injected with BO-112. Flow cytometry and multiplex tissue immunofluorescence were used to determine the effects on antitumor immunity. …”
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Feminization of Maroon Clownfish (<I>Amphiprion biaculeatus<I/>, Bloch 1790) with 17β-Estradiol Hormonal Induction by Helena Sahusilawane, Agus Oman Sudrajat, Muhmmad Agus Suprayudi, Dinar Tri Soelistyowati, Ligaya I. T. A. Tumbelaka, Irzal Effendi

“…Five fish composed of α-fish, β-fish, and three γ-fish were reared in each aquarium for 90 days with a flowing water system. The α- and β-fish were then removed, while the γ-fish was injected with hormone. …”
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WKB ameliorates DSS-induced colitis through inhibiting enteric glial cells activation and altering the intestinal microbiota by Qi Sun, Bai-Rong Li, Dong-Hao Li, Xiao-Ying Wang, Qian-Yi Wang, Zhi-Meng Jiang, Shou-Bin Ning, Tao Sun

“…Immune responses were measured using flow cytometry and cytokine profiling, while gut microbiota changes were examined through metagenomic sequencing. …”
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CXCR4 promotes tumor stemness maintenance and CDK4/6 inhibitors resistance in ER-positive breast cancer by Qiangfeng Shi, Wang Yang, Yiye Ouyang, Yujie Liu, Zijie Cai

“…The effects of CXCR4 knockdown and overexpression via siRNAs and plasmids transfection were examined by mammosphere formation, RT-qPCR, flow cytometry, MTT and colony formation assays. …”
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The Effect of Vascular Endothelial Growth Factor on Bone Marrow Mesenchymal Stem Cell Engraftment in Rat Fibrotic Liver upon Transplantation by Ke Yuan, Chunyou Lai, Lingling Wei, Tianhang Feng, Qinyan Yang, Tianying Zhang, Tao Lan, Yutong Yao, Guangming Xiang, Xiaolun Huang

“…The cell surface receptor profile of BMSC was examined by flow cytometry and immunofluorescence staining. Hepatic sinusoidal vascular leakage was measured with Evan’s blue dye assay. …”
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Investigating of density effect on the growth and survival of Caspian Kutum, Rutilus frisii (Kamensky, 1901) pre-fattening in freshwater concrete ponds by Alireza Valipour, Danial Gorouhi, Mohammad Sayad Bourani, Ali Hasanpour

“…The ponds received a constant water flow from an agricultural irrigation channel at the Restoration and Protection of Fish Genetic Resources of Shahid Ansari Center, Rasht, Iran. …”
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Stimulation of DC-CIK with PADI4 Protein Can Significantly Elevate the Therapeutic Efficiency in Esophageal Cancer by Chunyan Liu, Yingying Zheng, Junyi Tang, Dawei Wang, Zhenshen Ma, Shutong Li, Xiaotian Chang

“…DC and CIK cell phenotypes were determined using flow cytometry. The proliferation and viability of CIK cells were analyzed using trypan blue staining. …”
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Impact of Amarogentin on Gastric Carcinoma Cell Multiplication, Apoptosis and Migration via circKIF4A/miR-152-3p by Zhi Tan, Weining Wang, Jin Peng, Zhen Zhou, Jia Pan, Aiming Peng, Hui Cao, Wenling Fan

“…Cell multiplication, clone formation, as well as apoptosis and migration measurements were made by MTT, plate clone formation, flow cytometry and Transwell assays, respectively; Double luciferase reporter assay was performed for targeting relationship identification between circKIF4A and miR-152-3p; Western blots were carried out to measure Bax and Bcl-2 protein levels. …”
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Long-Term Homogeneity and Trends of Hydroclimatic Variables in Upper Awash River Basin, Ethiopia by Mekonnen H. Daba, Gebiaw T. Ayele, Songcai You

“…The data consist of 18 rainfall stations, 8 temperature stations, and 8 flow gauging stations across the UASB. Homogeneity and trends in streamflow, rainfall, and temperature variables were analyzed for the time period 1980 to 2017. …”
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Blockades of effector T cell senescence and exhaustion synergistically enhance antitumor immunity and immunotherapy by Xia Liu, Emily Shaw, Fusheng Si, Guangyong Peng, Yan Tao, Feiya Ma, David Bagley, Yuanqin Zhang

“…Furthermore, T cell senescence induced by mouse tumor and regulatory T (Treg) cells in vitro was determined with multiple markers and assays, including real-time PCR, flow cytometry, and histochemistry staining. Loss-of-function strategies with pharmacological inhibitors and the knockout mouse model were used to identify the potential molecules and pathways involved in T cell senescence. …”
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STING agonist-based treatment promotes vascular normalization and tertiary lymphoid structure formation in the therapeutic melanoma microenvironment by Walter J Storkus, Ronald J Fecek, Manoj Chelvanambi, Jennifer L Taylor

“…Treated and control tumors were isolated at various time points to assess transcriptional changes associated with VN and TLS formation via quantitative PCR (qPCR), with corollary immune cell composition changes in isolated tissues determined using flow cytometry and immunofluorescence microscopy. …”
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Reinforcing cancer immunotherapy with engineered porous hollow mycobacterium tuberculosis loaded with tumor neoantigens by Jie Jiang, Feng-Ying Huang, Shu-Zhen Dai, Guang-Hong Tan, Ming-Hui Chen, Hengyu Chen, Wu-Ping Zheng, Ri-Hong Wu, Weijing Xie

“…Fluorescence microscopy was used to detect PAMPs and adhesion molecules on phMTb as well as to observe its role in aiding dendritic cells (DCs) in antigen uptake into endosomes or lysosomes. Flow cytometry was used to assess the retention of PAMPs and adhesion molecules on phMTb, investigate antigen uptake by DCs, evaluate their activation and maturation status, examine the presentation of tumor neoantigens, and analyze immune cells in draining lymph nodes and tumor tissues. …”
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A novel TID + IDN controller tuned with coatis optimization algorithm under deregulated hybrid power system by Geetanjali Dei, Deepak Kumar Gupta, Binod Kumar Sahu, Mohit Bajaj, Vojtech Blazek, Lukas Prokop

“…Additionally, the study examines the system’s behaviour when incorporating energy storage units such as Redox Flow Batteries (RFB). Furthermore, the research investigates the system under various power transactions in a deregulated environment, considering generation rate constraints and governor dead bands. …”
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Exosomal miR-122 derived from M2 macrophages induces osteogenic differentiation of bone marrow mesenchymal stem cells in the treatment of alcoholic osteonecrosis of the femoral hea... by Guoping Le, Riyou Wen, Huaixi Fang, Zhifa Huang, Yong Wang, Hanwen Luo

“…M2 macrophages were identified by flow cytometry, and the isolated exosomes were characterized by transmission electron microscopy (TEM) and Nanoparticle Tracking Analysis (NTA). …”
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