Loop33 × 123 CAR-T targeting CD33 and CD123 against immune escape in acute myeloid leukemia by Haotian Ma, Zhifeng Yan, Runxia Gu, Yingxi Xu, Shaowei Qiu, Haiyan Xing, Kejing Tang, Zheng Tian, Qing Rao, Min Wang, Jianxiang Wang

“…Degranulation and cytokine secretion levels were detected by flow cytometry. Cell-derived xenograft models were established using wild-type Molm 13 cell lines, or a mixture of Molm 13-KO33 and Molm 13-KO123 cells as an ideal model of immune escape. …”
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РЕЄСТРАЦІЯ ПРАВА ІНТЕЛЕКТУАЛЬНОЇ ВЛАСНОСТІ НА ОСНОВІ ТЕХНОЛОГІЇ БЛОКЧЕЙН by Максим Вінник, Максим Полторацький, Ольга Коннова, Наталія Кушнір, Андрій Богданович

“…Нами було реалізовано смарт-контракт, який керує процесом створення та передачі  NFT з використанням мови Cadence на блокчейні Flow. Крім того, було реалізовано ключові функціональні вимоги до системи, а саме: реєстрація авторів та експертів (рецензентів), додання ключових слів для відбору експертів (рецензентів), пошук відповідних експертів за ключовими словами, реалізація функцій оплати рецензентам за виконану роботу. …”
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Human development II: We Need an Integrated Theory for Matter, Life and Consciousness to Understand Life and Healing by Sören Ventegodt, Tyge Dahl Hermansen, Maj Lyck Nielsen, Birgitte Clausen, Joav Merrick

“…The problems we address are all connected to the information flow in the functioning, living organism: function of the brain and consciousness, the regulations of the immune system and cell growth, the dynamics of health and disease. …”
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Genetic differentiation and historical dynamics of the endemic species Rheum pumilum on the Qinghai-Tibetan Plateau inferred from phylogeography implications by Shuliang Liu, Jianxin Gao, Bo Xiao, Wenjie Guo, Qiushi Yu, Ailan Wang, Weiwei Li

“…The total genetic diversity of Rh. pumilum is remarkably high (Ht = 0.910), with the majority of genetic variation primarily occurred among populations (84.5%) with limited gene flow, indicating geographic isolation influenced by diverse habitats of plateau. …”
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Preparation of noninfectious scRNAseq samples from SARS-CoV-2-infected epithelial cells. by Raven M Osborn, Justin Leach, Michelle Zanche, John M Ashton, ChinYi Chu, Juilee Thakar, Stephen Dewhurst, Sonia Rosenberger, Martin Pavelka, Gloria S Pryhuber, Thomas J Mariani, Christopher S Anderson

“…Fixed cells were tested for the presence of infectious SARS-CoV-2 virions using the Tissue Culture Infectious Dose Assay (TCID50), and also tested for viability using flow cytometry. We then tested the dissociation and methanol-acetone inactivation method on primary human lung epithelial cells that had been differentiated on an air-liquid interface. …”
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Selective expression and significance of ACKR2 in lung aerocytes by Alberto Mantovani, Gianluigi Condorelli, Raffaella Bonecchi, Massimo Locati, Marina Sironi, Roberta Carriero, Francesca Albano, Valeria Mollica Poeta, Lucia Zotti, Alessandra Castagna, Arianna Felicetta, Alessandro Mesaglio, Elisa Zaghen, Arianna Capucetti, Rachele Di Donato, Mattia Laffranchi, Matteo Massara, Silvano Sozzani

“…This study aims to explore the specific cell types expressing ACKR2 and their relative contributions to the protection against lung metastasis.Methods ACKR2 expression was studied by the generation of an inducible and conditional knockout (KO) mouse expressing two reporter genes, luciferase and TdTomato visible by In Vivo Imaging System, flow cytometry and immunofluorescence. Gene expression in lung endothelial cells (ECs) was investigated by RNA sequencing analysis. …”
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Effects of bronchoscopic alveolar lavage–assisted mechanical ventilation on postoperative pulmonary infection and inflammatory factors in patients undergoing lung cancer surgery by Hui Zhu, Haiyang Lu

“…Maximum minute ventilation, total lung capacity, forced expiratory volume in 1 second, and maximum mid-expiratory flow were substantially enhanced following each treatment. …”
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Hypothermia regulates mitophagy and apoptosis via PINK1/Parkin-VDAC 3 signaling pathway during oxygen-glucose deprivation/recovery injury by Luying Zhang, Song Yang, Hao Cui, Chenchen Hang, Xingsheng Wang, Le An, Zhenyu Shan, Zhen Liang, Rui Shao, Ziren Tang

“…Mitochondrial membrane potential (MMP) and mitochondrial permeability transition pore (mPTP) opening were determined respectively by JC-1 staining and BBcellProbe M61 staining using a flow cytometer. Expression of mitophagy-related proteins (Cleaved PINK1, Parkin, SQSTM1/p62, Beclin-1, LC3B II/LC3B I), apoptosis-related proteins (Bcl-2, Cytochrome C, caspase-3, cleaved caspase3) and VDAC3 were assessed using western blot analysis and quantitative real-time PCR. …”
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The Influence of Coupled Thermomechanical Processes on the Pressure and Temperature due to Cold Water Injection into Multiple Fracture Zones in Deep Rock Formation by Bruno Figueiredo, Chin-Fu Tsang, Auli Niemi

“…Changes in temperature were found not to be affected significantly by the thermomechanical and hydromechanical effects, but by the flow rate in the fracture zones. A sensitivity analysis was conducted to study the influence of the number, the initial permeability, the elastic modulus and the residual porosity of the fracture zones, and the elastic modulus of the confining intact rock, on the simulation results. …”
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Amantadine modulates novel macrophage phenotypes to enhance neural repair following spinal cord injury by Shijie Yang, Beibei Yu, Qing Zhang, Yongfeng Zhang, Longhui Fu, Bisheng Zhou, Haining Wu, Jianzhong Li, Shouping Gong

“…Subsequently, immunofluorescence staining, flow cytometry, and western blotting were performed to validate the identified effects of amantadine on macrophage differentiation and inflammation. …”
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Impact of Renewable Energy Sources on the Electricity Generation Cost by V. A. Liubchyk

“…The study involves scenario modeling based on the construction of energy chains that determine the flow of energy from the extraction and import of energy resources through technologies for transforming energy forms, transmission and distribution to the final consumer. …”
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Displacement of hydrocarbon liquids by water using the models of zonally heterogeneous deformable formations by Marat Ya. Khabibullin, Rustem I. Suleymanov, Razifa R. Stepanova, Alina Az. Gizzatullina, Arsen M. Khabibullin

“…In this paper, the averaging method and the Khristianovich function will be used to linearize the equations. To predict the well flow rate, an algorithm it is necessary to determine the noted reservoir parameters at any time. …”
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Murine DX5+NKT Cells Display Their Cytotoxic and Proapoptotic Potentials against Colitis-Inducing CD4+CD62Lhigh T Cells through Fas Ligand by Jens M. Werner, Michael Damian, Stefan A. Farkas, Hans J. Schlitt, Edward K. Geissler, Matthias Hornung

“…After polyclonal stimulation with anti-CD3, anti-CD28, and IL-2, proliferation of CD4+CD62Lhigh cells was assessed using a CFSE assay and activity of proapoptotic caspase-3 was determined by intracellular staining and flow cytometry. Extrinsic apoptotic pathway was blocked using an unconjugated antibody against FasL, and activation of caspase-3 was measured. …”
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Optimized Method to Generate Well-Characterized Macrophages from Induced Pluripotent Stem Cells by Qimin Hai, Peter Bazeley, Juying Han, Gregory Brubaker, Jennifer Powers, Claudia M. Diaz-Montero, Jonathan D. Smith

“…Using up to five independent iPSC donors, we performed several assays for macrophage functions and polarization, such as marker protein staining by flow cytometry, lipoprotein uptake, phagocytosis, cytokine release, inflammasome activation, and the effects of M1-like and M2-like polarization. …”
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Identification and validation of the important role of KIF11 in the development and progression of endometrial cancer by Biying Wang, Lunmin Bao, Xiaoduo Li, Guang Sun, Wu Yang, Nanzi Xie, Ling Lei, Wei Chen, Hailong Zhang, Man Chen, Xing Zhao, Xiufang Wan, Rui Yuan, Hongmei Jiang

“…KIF11 knockdown had inhibitory effects on cell proliferation, migration and invasion. Flow cytometry analysis revealed that KIF11 knockdown induced G2/M phase arrest and promoted apoptosis in EC cells. …”
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Combining theory and usability testing to inform optimization and implementation of an online primary care depression management tool by Nicola McCleary, Justin Presseau, Isabelle Perkins, Brittany Mutsaers, Claire E. Kendall, Janet Yamada, Katharine Gillis, Douglas Green

“…Usability issues were summarised within five themes: Optimizing content and flow to align with issues faced in practice, Enhancing the most useful algorithm components, Interactivity of the algorithm and embedded tools, Clarity of presence, purpose, or function of components, and Navigational challenges and functionality of links. …”
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CX3CR1+ age-associated CD4+ T cells contribute to synovial inflammation in late-onset rheumatoid arthritis by Mitsuhiro Akiyama, Sohma Wakasugi, Keiko Yoshimoto, Koichi Saito, Sho Ishigaki, Risa Inukai, Yoshiyuki Matsuno, Waleed Alshehri, Yasushi Kondo, Yuko Kaneko

“…Methods Fresh peripheral blood samples were collected from 78 treatment-naïve active RA patients, 12 with difficult-to-treat RA, and 16 healthy controls. Flow cytometry was employed to measure the proportions of CX3CR1+cytotoxic CD4+ and CD8+ T cells in these samples. …”
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Integration of artificial intelligence and advanced optimization techniques for continuous gas lift under restricted gas supply: A case study by Leila Zeinolabedini, Forough Ameli, Abdolhossein Hemmati-Sarapardeh

“…In the oil industry, gas lift is essential for facilitating fluid flow toward the production unit. However, the challenge lies in balancing gas availability constraints to achieve maximum efficiency in an oil field. …”
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Ultrasonographic evaluation of joint involvement in rheumatoid arthritis: Comparison with conventional radiography and correlation with disease activity parameters by Renu Saigal, Laxmikant Goyal, Hariram Maharia, Meenakshi Sharma, Abhishek Agrawal

“…After X-ray imaging (posterior-anterior view) of both hand joints, PD and gray scale US examination of 14 joints of both hands was performed and mean cumulative flow signal score (CFS) was calculated. Disease activity score (DAS28) was also calculated for each patient. …”
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Characterization of Local Nano-Heat Transfer Fluids for Solar Thermal Collection by Kawira Millien

“…The heat capacity of the steam in the heat exchanger was 93.7% dry and had a thermal capacity of 5.71 × 103 kJ. The heat rate of flow of the oil-based copper nano-heat transfer fluids was an average of 72.7 Js−1·kg−1 to 89.1 Js−1·kg−1. …”
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