Molecular mechanism underlying cardioprotective effect of dehydroepiandrosterone on endoplasmic reticulum stress induced apoptosis in human vascular smooth muscle cells and human u... by Ye Zhu, Junxiu Wei, Xin Yang, Wei Zhu

“…DHEA was utilized to inhibit the apoptotic effects of DTT, while estrogen receptor (ER) antagonists ICI 182,780 and G15, the androgen receptor (AR) antagonist flutamide and the aromatase inhibitor letrozole were used to identify the receptors activated during DHEA treatment in HVSMCs and HUVECs. Flow cytometry assessed the apoptotic rate, and Western blotting analysis evaluated the expression levels of ERS-related proteins GRP78 and PERK, and the apoptotic protein marker CHOP. …”
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circHUWE1 Exerts an Oncogenic Role in Inducing DDP-Resistant NSCLC Progression Depending on the Regulation of miR-34a-5p/TNFAIP8 by Xueliang Yang, Quan Sun, Yongming Song, Wenli Li

“…Cell apoptosis and cell cycle distribution were determined by flow cytometry. Migrated and invaded cell numbers were examined by transwell assay. …”
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Synthesized 18-Lead Electrocardiogram in Diagnosing Posterior Stemi-Equivalent Acute Coronary Syndrome in Patients with NSTEMI by Tomoki Horie, Rikuta Hamaya, Tomoyo Sugiyama, Hidenori Hirano, Masahiro Hoshino, Yoshihisa Kanaji, Tetsumin Lee, Taishi Yonetsu, Tetsuo Sasano, Tsunekazu Kakuta

“…LCx-culprit ACS was defined as thrombolysis in myocardial infarction (TIMI) 0–1 flow. The association between sV7-9 STE and myocardial damage was also assessed. …”
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Differentiation Potential of Early- and Late-Passage Adipose-Derived Mesenchymal Stem Cells Cultured under Hypoxia and Normoxia by Ashley G. Zhao, Kiran Shah, Julien Freitag, Brett Cromer, Huseyin Sumer

“…Furthermore, expression of these genes reduced with passage number, with the exception of PPARG, an adipose differentiation marker, possibly due to the adipose origin of the MSCs. We confirmed by flow cytometry the presence of cell surface markers CD105, CD73, and CD90 and lack of expression of CD45, CD34, CD14, and CD19 across all conditions. …”
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ITGA4 as a potential prognostic and immunotherapeutic biomarker in human cancer and its clinical significance in gastric cancer: an integrated analysis and validation by Jiaxing Zhang, Jiaxing Zhang, Gang Wang, Jie Liu, Futian Tang, Song Wang, Yumin Li, Yumin Li

“…However, its role across different cancer types is not well understood.MethodsUtilizing multi-omics data, we comprehensively evaluated ITGA4’s expression, clinical relevance, diagnostic and prognostic value, functions, mutations, and methylation status, along with its impact on immunity, mismatch repair (MMR), heterogeneity, stemness, immunotherapy responsiveness, and drug resistance in pan-cancer, with partial validation in gastric cancer (GC) using transcriptomic analysis, single-cell data, western blot (WB), wound-healing assay, flow cytometry and immunohistochemistry (IHC). We further investigated its correlation with clinicopathology and serological markers on tissues from 80 GC patients.ResultsITGA4 expression was generally low in normal tissues but varied significantly across tumor types, with higher levels in advanced stages and grades. …”
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How effective is the addition of specific exercise therapy for patients after anterior cruciate ligament surgery? A systematic review and meta-analysis by Hao Zhou, Jia Qian, Yu-Mei Xing, Long Cui, Yi-Feng Bu

“…Study quality was assessed using the Cochrane Risk-of-Bias tool.Study selectionA total of 11 randomized controlled trials (whole-body vibration training = 4, core-stability training = 2, strength training = 3, blood flow restriction training = 1, and aquatic training = 1) involving 552 anterior cruciate ligament surgery patients were included.Data extractionTwo researchers individually screened the key information for each eligible study and evaluated the quality of the studies. …”
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Cytotoxicity Potential of Endophytic Fungi Extracts from Terminalia catappa against Human Cervical Cancer Cells by Michèle Stella Majoumouo, Marius Belmondo Tincho, Rufin Marie Kouipou Toghueo, Thureyah Morris, Donavon Charles Hiss, Fabrice Fekam Boyom, Chitra Mandal

“…Furthermore, apoptosis was determined using the Annexin V/propidium iodide (PI) flow cytometry assay. Endophyte extracts N2, N7, N8, N97, N169, and N233 were obtained from Trichoderma sp, Phoma sp, Phomopsis phyllanticola, Fusarium oxyporum, Collectotrichum sp, and Cryptococcus flavescens, respectively. …”
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CircBAZ1B stimulates myocardial ischemia/reperfusion injury (MI/RI) by modulating miR-1252-5p/ATF3-mediated ferroptosis by Ruili Wei, Tianxiao Yang, Weihong Li, Xiqian Wang

“…Cell viability, apoptosis, lipid reactive oxygen species (ROS) levels and iron content were determined via Cell Counting Kit-8 (CCK-8) and flow cytometric assays. Gene and ferroptosis-related protein expression levels were verified by qRT‒PCR and Western blotting. …”
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Th9 Cells in Peripheral Blood Increased in Patients with Immune-Related Pancytopenia by Qing Shao, Yangyang Wang, Zhaoyun Liu, Hui Liu, Yihao Wang, Yang Zhao, Lijuan Li, Rong Fu

“…The percentage of Th9 cells was detected by flow cytometry (FCM) and ELISA. CD4+ lymphocytes were sorted by magnetic beads, and the mRNA expression levels of Th9 cells related transcription factors PU.1 and BATF were detected by RT-PCR. …”
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Alpha-synuclein pathology enhances peripheral and CNS immune responses to bacterial endotoxins by Anna-Sophia Hartke, Cara S. Schreiber, Kristina Lau, Ivo Wiesweg, Inken Waltl, Ulrich Kalinke, Franziska Richter, Christopher Käufer

“…To investigate this, we created a double-hit model based on the Thy1-aSyn mouse line (line 61), an established aSyn-overexpression model of PD, exposed to a single intraperitoneal injection of LPS at a dose of 0.8 mg/kg (equivalent to approximately 1,200,000 EU/kg). Clinical parameters, flow cytometry of blood and immune cells in the brain, brain immunohistology and motor behavior were evaluated over time. …”
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Age‐related longitudinal change in cardiac structure and function in adults at increased cardiovascular risk by Fei Fei Gong, Jennifer M. Coller, Michele McGrady, Umberto Boffa, Louise Shiel, Danny Liew, Simon Stewart, Alice J. Owen, Henry Krum, Christopher M. Reid, David L. Prior, Duncan J. Campbell

“…Age was associated with longitudinal increase in left ventricular (LV) mass index (LVMI); decrease in LV volumes; increase in LV ejection fraction; decrease in mitral annular systolic velocity; decrease in diastolic function (decreased mitral early diastolic annular velocity (e′); and increase in left atrial volume index, mitral peak early diastolic flow velocity (E)/e′ ratio, and tricuspid regurgitant velocity (TRVmax) in men and women, except for TRVmax in men). …”
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Cell culture expansion media choice affects secretory, protective and immuno-modulatory features of adipose mesenchymal stromal cell-derived secretomes for orthopaedic applications by Enrico Ragni, Andrea Papait, Michela Maria Taiana, Paola De Luca, Giulio Grieco, Elsa Vertua, Pietro Romele, Cecilia Colombo, Antonietta Rosa Silini, Ornella Parolini, Laura de Girolamo

“…Methods: Immunophenotype and secretory ability at soluble protein and EV-related levels, including embedded miRNAs, were analysed in the secretomes by means of flow cytometry, nanoparticle tracking analysis, high throughput ELISA and qRT-PCR arrays. …”
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Mapping factors influencing initiation of antiretroviral treatment among adolescents living with HIV/AIDS in sub-Saharan Africa: A scoping review protocol. by Palesa Grace Likoti, Desmond Kuupiel, Nelisiwe Khuzwayo

“…The selection process will be documented by following and using the PRISMA flow diagram (Fig 1). A thematic content analysis will present a narrative account of the extracted data.…”
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The Use of Trichostatin A during Pluripotent Stem Cell Generation Does Not Affect MHC Expression Level by Sara Farahi, Sara Hosseini, Hossein Ghanbarian, Seyed Mahmoud Hashemi, Mohammad Salehi, Samaneh Hosseini

“…The presence of MHC molecules on the surface of pluripotent stem cells was also checked by flow cytometry technique. Significant increase of pluripotency markers, oct4, nanog, sox2, and klf4, was observed in 100 nM TSA-treated samples. 100 nM TSA induced significant upregulation of H2db in generated iPSCs. …”
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Empagliflozin-Metformin Combination Has Antioxidative and Anti-Inflammatory Properties that Correlate with Vascular Protection in Adults with Type 1 Diabetes by Miodrag Janić, Matej Cankar, Jan Šmid, Alenka France Štiglic, Aleš Jerin, Mišo Šabovič, Andrej Janež, Mojca Lunder

“…., it improved endothelial function (measured by flow-mediated dilation and reactive hyperaemia index) and reduced arterial stiffness (measured as pulse wave velocity and local carotid artery stiffness) in adults with type 1 diabetes. …”
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Development of a TCR-like antibody and chimeric antigen receptor against NY-ESO-1/HLA-A2 for cancer immunotherapy by Xin Liu, Yang Du, Ningyan Zhang, Zhiqiang An, Wei Xiong, Yixiang Xu, Bingnan Yin, Yuqian Huang, Junjun Chu, Changsheng Xing, Chen Qian, Tianhao Duan, Helen Y Wang, John S. Yu, Rongfu Wang

“…The specificity and affinity of those antibodies were characterized by flow cytometry, ELISA, biolayer interferometry, and confocal imaging. …”
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One-Step Solid Extraction for Simultaneous Determination of Eleven Commonly Used Anticancer Drugs and One Active Metabolite in Human Plasma by HPLC-MS/MS by Shouhong Gao, Zhengbo Tao, Jingya Zhou, Zhipeng Wang, Yunlei Yun, Mingming Li, Feng Zhang, Wansheng Chen, Yejun Miao

“…Chromatographic separation was achieved on an Atlantis T3-C18 column (2.1 × 100 mm, 3.0 μm) with gradient elution using a mobile phase consisting of acetonitrile and 10 mM ammonium acetate plus 0.1% formic acid in water, and the flow rate was 0.25 mL/min. The Agilent G6410A triple quadrupole liquid chromatography-mass spectrometry system was operated under the multiple reaction monitoring mode with an electrospray ionization in the positive mode. …”
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Therapeutic Effect of Gypenosides on Antioxidant Stress Injury in Orbital Fibroblasts of Graves’ Orbitopathy by Chao Ma, Haoyu Li, Wei Liu, Shuwen Lu, Xian Li, Jinyuan Chen, Kaijun Li, Wenzhan Wang

“…ROS and SOD test suites were employed to detect the oxidative stress level in OFs. Flow cytometry apoptosis detection, TUNEL detection, and lactate dehydrogenase detection were used to analyze the level of apoptosis. …”
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Research on leakage and diffusion behavior of hydrogen doped natural gas in integrated pipeline corridors based on data drive by Xiaolong Gong, Hua Li, Cheng Li, Mingyue Kou, Lingxu Kong, Hongcheng Liu

“…In the case of emergency, the reasonable design of Distance between inlet and outlet and air flow configuration can help to quickly discharge harmful gases, ensure the safe evacuation and equipment maintenance in the pipe corridor, and have guiding significance for the ventilation design of the pipe corridor.…”
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Soil phosphorus mobilization as affected by long-term organic fertilization in a tropical Regosol by Kalline De Almeida Alves Carneiro, Tancredo Augusto Feitosa De Souza, Gislaine Dos Santos Nascimento, Vânia Da Silva Fraga, Bruno de Oliveira Dias

“…This study aimed to evaluate the PM in a tropical Regosol by using soil columns (10 cm depth) during steady saturated water flow and PM was correlated with Fe and Al oxides. …”
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