Targeting NF-kappaB-inducing kinase shapes B-cell homeostasis in myasthenia gravis by Xiaoyu Huang, Zhouao Zhang, Zhouyi Wang, Tiancheng Luo, Mingjin Yang, Xinyan Guo, Xue Du, Tianyu Ma, Yong Zhang

“…The hallmark gene NF-kappaB-inducing kinase (NIK/MAP3K14) associated with NF-κB and TNF signaling was identified, and the expression levels of NIK in CD19+B cells, CD4+T cells and serum from new-onset MG patients and controls were validated by flow cytometry, qPCR and ELISA. In vitro and in vivo, the effects of NIK inhibitor (B022) on the function of CD19+B cells and CD4+T cells were detected under the MG PBMCs, sorted B cells and experimental autoimmune MG (EAMG) rat model, respectively. …”
Get full text

PD-L1 positive platelets mediate resistance to immune checkpoint inhibitors in patients with colorectal cancer by Jiacheng Li, Jia Liu, Shifeng Yang, Yu Xia, Qingzhe Meng, Biying Sun, Yansong Liu, Bin Zhao, Jiaqi Jin, Hui Xu, Lihong Wang, Pengxia Zhang, Zhuoxin Cheng

“…Methods The functional depletion effects of PD-L1 positive platelets on TME and immune cells were measured via western blotting, immunofluorescence staining, qRT-PCR, ELISpot and flow cytometry. In vivo, CD274 knockout (KO), CD8a KO, platelet-specific KO (PF4-Cre-Hsp90b1flox/flox) mouse models and a subcutaneous tumour model treated with aspirin and PD-L1 mAb were established in C57BL/6 N mice. …”
Get full text

The Diagnostic Capability of Swept Source OCT Angiography in Treatment-Naive Exudative Neovascular Age-Related Macular Degeneration by Daniel Ahmed, Martin Stattin, Anna-Maria Haas, Stefan Kickinger, Maximilian Gabriel, Alexandra Graf, Katharina Krepler, Siamak Ansari-Shahrezaei

“…In 23 of these 32 eyes (71.9%), abnormal flow in cross-sectional SS-OCTA B-scans was identified, giving an overall SS-OCTA sensitivity of 94.3%. …”
Get full text

Magnetic Resonance Imaging of Contrast-Induced Acute Renal Injury and Related Pathological Alterations In Vivo by Yanfei Li, Dafa Shi, Haoran Zhang, Xiang Yao, Ke Ren

“…The definitive mechanisms of CI-AKI include contrast medium (CM) nephrotoxicity and CM disturbances in renal blood flow, but how the immune system responds to CM has rarely been mentioned in previous studies, and different cell death pathways have not been clearly distinguished. …”
Get full text

Strongly Coupled 𝒫𝒯-Symmetric Models in Holography by Daniel Areán, David Garcia-Fariña, Karl Landsteiner

“…Remarkably, these non-Hermitian lattices flow to a <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi mathvariant="script">PT</mi></semantics></math></inline-formula>-symmetric fixed point in the IR.…”
Get full text

The Promising Effect of Tocilizumab on Chronic Antibody-Mediated Rejection (cAMR) of Kidney Transplant by Łukasz Świątek, Miłosz Miedziaszczyk, Dominik Lewandowski, Filip Robakowski, Piotr Tyburski, Marta Jakubowska, Marek Karczewski, Ilona Idasiak-Piechocka

“…<b>Methods</b>: A systematic review was performed using the PRISMA 2020 Checklist and Flow diagram. A systematic review protocol was registered in PROSPERO: CRD42024510996. …”
Get full text

Mapping evidence on cryptococcal antigen infection among HIV-infected persons in sub-Saharan Africa- A scoping review protocol. by Khululiwe Dlamini, Boitumelo Moetlhoa, Astrid Turner, Kuhlula Maluleke, Tivani Mashamba-Thompson

“…Point-of-care testing has high sensitivity and specificity as seen with the cryptococcal antigen lateral flow assay screening to expedite treatment. The aim of the study is to map and translate evidence on cryptococcal antigen infection among HIV-infected persons in sub-Saharan Africa.…”
Get full text

Vδ2 T-cells response in people with Mpox infection: a three-month longitudinal assessment by Eleonora Cimini, Eleonora Tartaglia, Francesco Messina, Andrea Coppola, Valentina Mazzotta, Massimo Tempestilli, Giulia Matusali, Stefania Notari, Annalisa Mondi, Gianluca Prota, Alessandra Oliva, Carla Fontana, Enrico Girardi, Fabrizio Maggi, Andrea Antinori

“…Vδ2 T-cell profiles (CD45RA/CCR7), activation/exhaustion markers (CD38/HLA-DR/CD57/PD-1/TIM-3), cytokine production (IFN-γ/TNF-α) and CD107a expression were assessed by multiparametric flow cytometry. Ten healthy donors (HD) were used as a control group. …”
Get full text

Jianpi Yiqi Busui prescription alleviates myasthenia gravis by regulating Th17 through the TAK1/P38 MAPK/eIF-4E signaling pathway by Zhuming Chen, Jing Lu, Tianying Chang, Dongmei Zhang, Yibin Zhang, Miao Liu, Tong Wu, Peng Xv, Jian Wang

“…Changes in body weight, forelimb grip strength, Lennon clinical score, and antifatigue ability of EAMG model rats were recorded to evaluate the effectiveness of JYBP. Flow cytometry was utilized to count Th17, Th1, Th2, and Treg cells in lymphocytes. …”
Get full text

MFG-E8 Exerts Neuroprotection in Neural Stem Cells Induced by Anesthetic Sevoflurane via Regulating the PI3K/AKT Pathways by Minmin Cai, Liufang Sheng

“…The cell viability and apoptosis affected by rhMFG-E8 were tested by MTT and flow cytometry analysis, respectively. Then, the mRNA expression of MFG-E8 was detected by qRT-PCR. …”
Get full text

Adipose-derived stem cells promote the recovery of intestinal barrier function by inhibiting the p38 MAPK signaling pathway by Mei Yang, Wangbin Xu, Chaofu Yue, Rong Li, Xian Huang, Yongjun Yan, Qinyong Yan, Shisheng Liu, Yuan Liu, Qiaolin Li

“…In addition, cell viability and apoptosis was determined by a CCK-8 kit and flow cytometry, respectively. Cell permeability was assayed by the transepithelial electrical resistance value and FITC-dextran concentration. …”
Get full text

Preliminary Study by Differential Scanning Calorimetric Analysis of Red Blood Cells in Peripheral Artery Disease Patients Treated with Cilostazol: Correlation with Improvements in... by Dénes Lőrinczy, Dorottya Szabó, László Benkő

“…<b>Objective</b>: Peripheral artery disease (PAD) is a prevalent vascular condition characterized by arterial narrowing, which impairs blood flow and manifests as intermittent claudication, a pain or cramping sensation induced by physical activity or ambulation. …”
Get full text

Investigation of Flooding due to Dam Failure: A Case Study of Darlık Dam by Ezgi Selen Tilav, Sezar Gülbaz

“…Within the scope of the study, flood flow, water velocity and water depth were calculated in case of possible dam failure. …”
Get full text

A quality improvement initiative to improve growth monitoring of children attending immunization clinic in an urban primary health centre in Delhi by Ravneet Kaur, Udita Singh, K. Srinath, Anjusha Ranjith, Baridalyne Nongkynrih

“…The baseline coverage of weight-for-age growth chart plotting in the immunization clinic was 31%. The process flow was mapped, and critical gaps identified by root cause analysis (Fish Bone technique). …”
Get full text

Sickle Cell Anemia Patients Display an Intricate Cellular and Serum Biomarker Network Highlighted by TCD4+CD69+ Lymphocytes, IL-17/MIP-1β, IL-12/VEGF, and IL-10/IP-10 Axis by Nadja Pinto Garcia, Alexander Leonardo S. Júnior, Geyse Adriana S. Soares, Thainá Cristina C. Costa, Alicia Patrine C. dos Santos, Allyson Guimarães Costa, Andréa Monteiro Tarragô, Rejane Nina Martins, Flávia do Carmo Leão Pontes, Emerson Garcia de Almeida, Erich Vinícius de Paula, Olindo Assis Martins-Filho, Adriana Malheiro

“…Immunophenotyping of cells, Toll-like receptors, and adhesion molecules were performed from peripheral blood samples of SCA patients and healthy donors by flow cytometry and cytokine/chemokine/growth factor measurement by the Luminex technique performed from the serum of the same subjects. …”
Get full text

Anti-Inflammatory and Healing Activity of the Hydroalcoholic Fruit Extract of Solanum diploconos (Mart.) Bohs by Larissa Benvenutti, Roberta Nunes, Ivonilce Venturi, Silvia Aparecida Ramos, Milena Fronza Broering, Fernanda Capitanio Goldoni, Sarah Eskelsen Pavan, Maria Verônica Dávila Pastor, Angela Malheiros, Nara Lins Meira Quintão, Elizabeth Soares Fernandes, José Roberto Santin

“…In vitro fMLP-induced chemotaxis, LPS-induced inflammatory mediator levels (cytokines by ELISA and NO release by Griess reaction), and adhesion molecule expression (CD62L, CD49d, and CD18, by flow-cytometry) were assessed in neutrophils treated with different concentrations of the extract. …”
Get full text

Potential of Hydrogen Fuel Cell Aircraft for Commercial Applications with Advanced Airframe and Propulsion Technologies by Stanislav Karpuk, Yannik Freund, Richard Hanke-Rauschenbach

“…Future technologies considered in the present work include laminar flow control, active load alleviation, new materials and structures, ultra-high bypass ratio turbofan engines, more efficient thermal management systems, and superconducting electric motors. …”
Get full text

Three Years of High Time-resolution Air Pollution Monitoring in the Complex Multi-source Harbor of New York and New Jersey by Gayle Hagler, Dan Birkett, Ronald C. Henry, Richard E. Peltier

“…The study area includes an international airport, interstate highway, port terminals and shipping lanes, and industrial sources, as well as typical urban emissions of a megacity. Because air flow travel time from sources to the monitor were usually much less than one hour, minute-by-minute, high-precision data were collected for three years (2013–2015) for sulfur dioxide (SO2), carbon monoxide (CO), oxides of nitrogen (NO, NO2), black carbon (BC), fine particulate matter (PM2.5), and meteorology (wind speed, wind direction, temperature, humidity). …”
Get full text

Development and Application of Well-Test Model after Injection Biological Nanomaterials by Qing Feng, Ping Gao, Xianchao Chen, Jiang Li, Jingchao Zhou, Feng Qian, Shengsheng Li, Yanni Sun, Yingying Wang

“…In this paper, the influence of the injection amount of bionanomaterials on the permeability is studied, and the rule of the permeability around the injection well with the distance from the bottom of the well is analyzed. On this basis, the flow mathematical model of three zones (inner zone, transition zone, and outer zone) of bionanosolution is established. …”
Get full text

Embodied climate impacts in urban development: a neighbourhood case study by Simon Sjökvist, Nicolas Francart, Maria Balouktsi, Harpa Birgisdottir

“…Urban planning significantly influences material flows, with a substantial portion of construction occurring in planned urban development areas (UDAs), such as 76% in Copenhagen, Denmark. …”
Get full text