Cultivatable Bacteriota of Chronic Wound of Patients with Diabetic Foot Syndrome with Critical Limb Ischemia Based on Wound Biopsy in Peri-Revascularization Period by Syedah Sarah Hussaini, Fran Dominic Grubesa, Mateusz Gajda, Martyna Schönborn, Katarzyna Bogucka, Mikołaj Maga, Paweł Maga, Jadwiga Wójkowska-Mach

“…Optimizing clinical environments (enhancing blood flow and controlling inflammation) in general over focusing singularly on microbiota composition or revascularization seems to be crucial and arguably outweighed the impact of microbial change alone; in particular, reperfusion may increase the conditions to bacterial growth at the first stage.…”
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Increased CCR7loPD-1hiCXCR5+CD4+ T Cells in Peripheral Blood Mononuclear Cells Are Correlated with Immune Activation in Patients with Chronic HBV Infection by Ya-Xin Huang, Qi-Yi Zhao, Li-Li Wu, Dong-Ying Xie, Zhi-Liang Gao, Hong Deng

“…The expression of CD4, CXCR5, PD-1, and CCR7 was detected in T cells from peripheral blood by flow cytometry. The frequency of the CCR7loPD-1hi T subset was significantly higher in the patients than in the healthy controls (14.92±4.87% vs 12.23±2.95%, p=0.018). …”
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Oncolytic peptide LTX-315 induces anti-pancreatic cancer immunity by targeting the ATP11B-PD-L1 axis by Meng Wang, Xing Huang, Tingbo Liang, Tianyu Tang, Gang Zhang, Minghao Lu, Zhengtao Hong, Junming Huang, Xiao Zhi

“…This study aimed to demonstrate the potential effect and mechanism of LTX-315 in PD-L1 inhibition-induced anti-pancreatic cancer immunity.Methods Both immunodeficient and immunocompetent mouse models were used to evaluate the therapeutic efficacy of monotherapy and combination therapy. Flow cytometry and immunohistochemistry were used to assess the immune microenvironment. …”
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Notch Signaling Pathway Promotes Th17 Cell Differentiation and Participates in Thyroid Autoimmune Injury in Experimental Autoimmune Thyroiditis Mice by Hao Liu, Yiwen Li, Yujiao Zhu, Lei Ma, Haibo Xue

“…HE staining, IHC staining, flow cytometry, RT-qPCR, and ELISA were used to evaluate the degrees of thyroiditis, detect the percentage of Th17 cells and measure the expression of retinoic acid-related orphan receptor gamma t (RORγt), interleukin-17A (IL-17A), and the main components of the Notch signaling pathway. …”
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Experiences of and response to the COVID-19 pandemic at private retail pharmacies in Kenya: a mixed-methods study by Edwine Barasa, Sassy Molyneux, Jacinta Nzinga, Audrey Mumbi, Peter Mwangi Mugo, Daniella Munene, Collins Jaguga, Davy Odhiambo, Elizabeth Ominde-Ogaja, Mercy Maina, Michael Mungoma, Nadia Butt, Sultani Matendechero, Titus Kahiga, Eric Sedah, Sylvia Opanga

“…The initial weeks of the pandemic were characterised by fear and panic among service providers and a surge in client flow. Subsequently, 65 (60%) of 108 pharmacies experienced a dip in demand to below prepandemic levels and 34 (31%) reported challenges with unavailability, high price and poor quality of products. …”
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Optimal multiobjective design of an autonomous hybrid renewable energy system in the Adrar Region, Algeria by Abderrahmane Mehallou, Benalia M’hamdi, Abderrahmane Amari, Madjid Teguar, Abdelaziz Rabehi, Mawloud Guermoui, Amal H. Alharbi, El-Sayed M. El-kenawy, Doaa Sami Khafaga

“…To achieve this, the proposed methods are combined with energy management strategy (EMS) rules that coordinate energy flows between the various system components. The findings reveal that the MOPSO method has the most efficient hybrid renewable configuration with an annual generation cost of electricity (COE) of 0.2520 $/kWh and loss of power supply probability (LPSP) of 9.164%, which dominates the performance of MOALO (COE of 0.1625$/kWh and LPSP of 8.4872%), MOGA (COE of 0.1577$/kWh and LPSP of 10%), and MODA (COE of 0.02425$/kWh and LPSP of 7.8649%). …”
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Understanding North Korea: Rimjin-gang Citizen Journalists Out to Cure the “Sick Man of Asia”? by Suzy Kim

“…Not only does it claim to be "the first magazine about North Korea written by North Koreans" (The Nation), but it is also touted as "the first publication to create a channel for two-way communication between the divided Korean people," as exemplified by the title "Rimjin-gang" - the name of the river that flows across the De-Militarized Zone dividing the north and south.This article is an attempt to engage in a critical conversation about how we know what we know about North Korea, reflecting upon the historical and structural conditions that configure the way North Korea is known and understood by those outside North Korea.…”
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Disturbed shear stress promotes atherosclerosis through TRIM21‐regulated MAPK6 degradation and consequent endothelial inflammation by Feng Wang, Shu‐Yu Wang, Yue Gu, Shuai Luo, Ai‐Qun Chen, Chao‐Hua Kong, Wen‐Ying Zhou, Li‐Guo Wang, Zhi‐Mei Wang, Guang‐Feng Zuo, Xiao‐Fei Gao, Jun‐Jie Zhang, Shao‐Liang Chen

“…Conclusions Our study illuminates the advantages of MAPK6 in decelerating plaque progression, highlighting the potential of safeguarding MAPK6 as a novel therapeutic strategy against atherosclerosis. Key points Disturbed flow activates the ubiquitin‒proteasome degradation pathway of MAPK6 in endothelial cells, which is contingent on the binding of the ubiquitin ligase TRIM21 to MAPK6. …”
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« L’épopée » des clandestins by Zied Hadfi

“…Kicking in, sometimes, suicidal adventures where they are told that the land flowing with milk and honey, starting from the countries that are still not at the status of "International beggars ». …”
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miR-223-3p Inhibits Antigen Endocytosis and Presentation and Promotes the Tolerogenic Potential of Dendritic Cells through Targeting Mannose Receptor Signaling and Rhob by Hao-Cheng Tang, Yin-Yan Lai, Jing Zheng, Hong-Yan Jiang, Geng Xu

“…FITC-OVA antigen uptake and cell surface markers in bone marrow-derived DCs (BMDCs) were analyzed by flow cytometry. BMDCs were transfected with the miR-223-3p mimic or inhibitor. …”
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Low-Dose Irradiation Differentially Impacts Macrophage Phenotype in Dependence of Fibroblast-Like Synoviocytes and Radiation Dose by Lisa Deloch, Jana Fuchs, Michael Rückert, Rainer Fietkau, Benjamin Frey, Udo S. Gaipl

“…For this, the bone marrow of hTNF-α tg mice was differentiated either with cytokines to obtain key macrophage phenotypes (M0, M1, and M2) or with supernatants (SN) of untreated or irradiated FLS. Flow cytometry analyses were used to analyse the impact of radiation (0.1, 0.5, 1.0, and 2.0 Gy) on the phenotype of macrophages in the presence or absence of SN of FLS. …”
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Relationships between muscle strength, lung function, and cognitive function in Chinese middle-aged and older adults: A study based on the China health and retirement longitudinal... by Yixuan Ma, Xinze Wu, Yinjiao Zhao, Weihao Hong, Yisheng Luan, Peiyu Song, Bing Zhang

“…Muscle strength was measured using normalized grip strength and chair-standing time, while lung function was evaluated using peak expiratory flow (PEF). Results: Total cognitive function scores exhibited significant correlations with grip strength, chair-standing time, and PEF. …”
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Supercritical CO2 Brayton Cycle Design for Small Modular Reactor with a Thermodynamic Analysis Solver by Pan Wu, Chuntian Gao, Yanping Huang, Dan Zhang, Jianqiang Shan

“…Increasing the dry cooling flow rate will be helpful to decrease the compressor inlet temperature. …”
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Inspiratory muscle training facilitates liberation from mechanical ventilation in subacute critically ill patients—a randomized controlled trial by Shu-Jane Wang, Tien-Pei Fang, Tien-Pei Fang, Daniel D. Rowley, Nan-Wei Liu, Jui-O Chen, Jui-Fang Liu, Jui-Fang Liu, Hui-Ling Lin, Hui-Ling Lin, Hui-Ling Lin

“…IMT intervention significantly reduced rapid shallow breathing index and improved respiratory muscle strength, with greater maximum inspiratory pressure (p < 0.01), maximum expiratory pressure (p = 0.03), and peak expiratory flow (p = 0.01). A moderate positive correlation was observed between IMT and increased creatinine levels (rs = 0.54, p = 0.01), whereas the no-IMT group showed a reduction.ConclusionIMT significantly shortened ventilator duration and improved respiratory muscle strength. …”
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Influence of injection technique, drug formulation and tumor microenvironment on intratumoral immunotherapy delivery and efficacy by Patrick Hwu, Ravi Murthy, Aung Naing, Sapna P. Patel, Simon Young, Alda Tam, Nina M. Muñoz, Malea Williams, Katherine Dixon, Crystal Dupuis, Amanda McWatters, Rony Avritscher, Soraya Zorro Manrique, Kevin McHugh, David Leach, Jeffrey D. Hartgerink, Punit Prakash, Rahul A. Sheth

“…The influence of varying needle design and hydrogel-based drug delivery on the immune response to a stimulator of interferon genes (STING) agonist was evaluated using flow cytometry and single cell RNA sequencing. We also evaluated the influence of tumor stiffness on drug injection distribution.Results Variations in needle design, specifically with the use of a multiside hole needle, led to approximately threefold improvements in intratumoral drug deposition relative to conventional end-hole needles. …”
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Isolation and Characterization of an Adult Stem Cell Population from Human Epidural Fat by Nedaa Al-Jezani, Roger Cho, Anand O. Masson, Brian Lenehan, Roman Krawetz, Frank G. Lyons

“…The cell surface receptor profile of HEF cells was examined by flow cytometry. HEF cells were also assayed through the collagen contraction assay. …”
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Mechanisms of N₂O Emission in Drip-Irrigated Saline Soils: Unraveling the Role of Soil Moisture Variation in Nitrification and Denitrification by Fang Zhang, Zhi Qu, Qian Zhao, Zixuan Xi, Zhen Liu

“…Therefore, during drip irrigation, the position and flow rate of the dripper should be controlled to reduce soil water saturation areas, maintain soil aeration, control soil moisture content below field holding capacity, promote the nitrification process, reduce N₂O emissions, and improve soil nitrogen use efficiency. …”
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Law enforcement activity, globalization and social morality in frameworks of mass culture (On the Example of Modern European TV-Shows on Law-Enforcements. Part 2) by Vovk V.

“…Special attention is paid to analyzing the sociocultural context within which modern mass culture exists (globalization, the spread of democracy, unification and standardization of life, compression of time, significant information flow, clip type of thinking, etc.). Results: the article shows that the spread of mass culture is due to the development of technologies and a change in the type of communication: from verbal-written to audiovisual (which only confirms the fundamental importance of sight and visual metaphor within European culture). …”
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Mitigating effects of Jiawei Chaihu Shugan decoction on necroptosis and inflammation of hippocampal neurons in epileptic mice by Qin Wang, Baijun Qin, Han Yu, Jiawei Zeng, Jingjing Fan, Qiong Wu, Rong Zeng, Haichun Yu, Xian Zhang, Mingfen Li, Yanying Zhou, Limei Diao

“…Viability and apoptosis were assessed via CCK-8 and flow cytometry. In vivo, 60 C57BL/6J mice were divided into six groups: control, model, carbamazepine, JWCHSGD, JWCHSGD + Sh Circ_Csnk1g3, and JWCHSGD + Sh NC. …”
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Pyrolysis-catalytic gasification of plastic waste for hydrogen-rich syngas production with hybrid-functional Ni-CaOCa2SiO4 catalyst by Tian Heng Qin, Guozhao Ji, Boyu Qu, Alan J McCue, Shaoliang Guan, Jos Derksen, Ye Shui Zhang

“…The different parameters (Ni loading, temperature, N2 flow rate and feedstock-to-catalyst ratio) have been investigated to optimise the H2 production. …”
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