Correlation of precisely fabricated geometric characteristics of DNA-origami nanostructures with their cellular entry in human lens epithelial cells by Yexuan Guo, Tianze Xiong, Hong Yan, Rui Xue Zhang

“…Atomic force microscopy and agarose gel electrophoresis showed that four DONs self-assembled within 3.5h with up to 59% yield and exhibited structural intactness in cell culture medium for 4 h. Flow cytometry analysis of four Cy5-labelled DONs in hLECs HLE-B3 found time-dependent cellular uptake over 2 h, among which the octahedron and triangle had higher cellular accumulation than the rod and ring. …”
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Enhancing Cervical Cancer Classification: Through a Hybrid Deep Learning Approach Integrating DenseNet201 and InceptionV3 by Abhiram Sharma, R. Parvathi

“…The experimental setup utilizes Python, TensorFlow, and Keras within a GPU-enabled environment to handle computational demands effectively. …”
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Enucleated bone marrow-derived mesenchymal stromal cells regulate immune microenvironment and promote testosterone production through efferocytosis by Lu Sun, Jiayu Huang, Xuezi Wang, Peng Huang, Baolin Dong, Zehang Liang, Jiahong Wu, Jiancheng Wang

“…The organelles and cellular functions of enucleated BMSCs were analyzed by immunofluorescence staining and flow cytometry. Extracellular vesicles (EVs) were isolated by ultracentrifugation and characterized. …”
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DKK3 promotes renal fibrosis by increasing MFF-mediated mitochondrial dysfunction in Wnt/β-catenin pathway-dependent manner by Jianling Song, Yanxia Chen, Yan Chen, Minzi Qiu, Wenliu Xiang, Ben Ke, Xiangdong Fang

“…Oxidative stress and mitochondria function were also evaluated. CCK-8 and flow cytometry were utilized to assess cell viability and apoptosis. …”
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Humoral and Cellular Immune Regulation in Albino Rat Exposed to Glyphosate and Aluminum Phosphide by Ujuamala Ezeani, Onyemaechi Afonne, Michael Ezeani

“…This assessment was conducted using flow cytometry (Cyflow).Results: The results indicated that the pre-exposure stage exhibited a CD4+ to CD8+ ratio of 2:1. …”
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The composition of peripheral immunocompetent cell subpopulations and cytokine content in the brain structures of mutant Disc1-Q31L mice by M. M. Gevorgyan, S. Ya. Zhanaeva, E. L. Alperina, T. V. Lipina, G. V. Idova

“…This is the first study to examine the immune parameters in Disc1-Q31L mice with a point mutation in the second exon of the DISC1 gene compared to mice of the C57BL/6NCrl strain (WT, wild type). A flow cytometry assay has shown that intact Disc1-Q31L mice differ from the WT strain by an increase in the percentage of CD3+ T cells, CD3+CD4+ Т helper cells and CD3+CD4+CD25+ T regulatory cells and a decrease in CD3+CD8+ T cytotoxic/suppressor cells in the peripheral blood. …”
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Inotodiol induces hepatocellular carcinoma apoptosis by activation of MAPK/ERK pathway. by Yushuang Xing, Di Jia, Xinping Zhu, Jialu Yang, Zhipeng Gao, Nana Meng, Haohao Xu, Mengxiao Wang, Shijun Chang, Mingqian Zhao, Shanbo Zhang, Zichen Mu, Qiang Tang, Weiming Zhao

“…The CCK8 assay was utilized to assess cell viability, the scratch assay was utilized to detect migration ability, the clone formation assay was utilized to detect clonogenic ability, and flow cytometry was utilized to analyze apoptosis and cell cycle. …”
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Developing Ecological Landscape Design Proposals Against Flood Susceptibility in Urban Areas: Antalya, Kemer Example by Onur Aksoy, Elif Altaş, Kamil Erken

“…With the proposed ecological landscape design approaches, the water flow will be regulated with plant-based applications, while the groundwater will be fed by increasing open green areas and permeable areas. …”
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Measurable residual disease as predictor of post–day +100 relapses after allografting in adult AML by Naveed Ali, Megan Othus, Eduardo Rodríguez-Arbolí, Corentin Orvain, Filippo Milano, Brenda M. Sandmaier, Chris Davis, Ryan S. Basom, Frederick R. Appelbaum, Roland B. Walter

“…Abstract: Measurable residual disease (MRD) by multiparametric flow cytometry (MFC) before allogeneic hematopoietic cell transplantation (HCT) identifies patients at high risk of acute myeloid leukemia (AML) relapse, often occurring early after allografting. …”
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Evaluation of diastole by echocardiography for detecting early cardiac dysfunction: an outcome study by Tatiana Kuznetsova, Nicholas Cauwenberghs, František Sabovčik, Yukari Kobayashi, Francois Haddad

“…We measured left heart dimensions, strain, tricuspid regurgitation, transmitral blood flow, and mitral annular tissue velocities using conventional echocardiography and Doppler imaging. …”
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Design and Optimization of a Hybrid Gas Generator for Hydrogen Peroxide Tank Pressurization by Mykhailo Vorobei, Mykola Bondarenko

“…Ceramic coatings and heat-resistant materials reduce erosion, and liquid oxygen flow control optimizes combustion. Aluminum addition to the fuel boosts specific impulse by 25 seconds. …”
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Overexpression of PAX8-AS1 Inhibits Malignant Phenotypes of Papillary Thyroid Carcinoma Cells via miR-96-5p/PKN2 Axis by Ping Zhou, Tongdao Xu, Hao Hu, Fei Hua

“…Cell proliferation and apoptosis capacities were assessed by MTT and flow cytometry, respectively. EdU assay was used to detect cell proliferation. …”
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A Circulating Subpopulation of Monocytic Myeloid-Derived Suppressor Cells as an Independent Prognostic/Predictive Factor in Untreated Non-Small Lung Cancer Patients by Eleni-Kyriaki Vetsika, Filippos Koinis, Marianthi Gioulbasani, Despoina Aggouraki, Anna Koutoulaki, Eirini Skalidaki, Dimitris Mavroudis, Vassilis Georgoulias, Athanasios Kotsakis

“…The percentages and correlation between MDSCs and distinct immune cells in the peripheral blood of 110 chemotherapy-naive patients before treatment and healthy controls were investigated using flow cytometry. Two monocytic [CD14+CD15−CD11b+CD33+HLA-DR−Lin− and CD14+CD15+CD11b+CD33+HLA-DR−Lin−] and a granulocytic [CD14−CD15+CD11b+CD33+HLA-DR−Lin−] subpopulations of MDSCs were identified, expressing inducible nitric oxide synthase, and reactive oxygen species, respectively. …”
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In vitro antitumor effects of methanolic extracts of three Ganoderema mushrooms by Elshahat A. Toson, Amira A. El-Fallal, Marwa A. Oransa, Hoda M. El-Gharabawy

“…In this regard, Sulfo-rhodamine B staining and flow cytometry were used. Hepatocellular carcinoma (HepG2) and breast ductal carcinoma (T-47D) cell lines were used as cancer models, while mouse normal liver (BNL) and oral epithelial cell (OEC) lines were used as respective controls. …”
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Evaluating the Impact Performance of Environmentally Friendly Asphalt Concrete Slabs Containing Different Proportions of Recycled Concrete Aggregate and Corrugated Steel Fibers by Walid Mansour, Diaa Ashraf, Ali Basha

“…To ensure the improvement of the asphalt mixtures' properties in terms of Marshall stability, flow, bulk density, air void ratio, and splitting tensile strength, steel fibers were added at a volume fraction of 1.0%. …”
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Periodontitis promotes tumor growth and immune evasion via PD-1/PD-L1 by Suli Wang, Fujiao Nie, Qiuyue Yin, Haoyang Tian, Pizhang Gong, Jinhong Ju, Jiayi Liu, Pishan Yang, Chengzhe Yang

“…Tumor volume and weight were recorded and the levels of relevant immune-suppressive cells and T cells were detected by flow cytometry or immunofluorescence. THP-1 cells were stimulated using conditioned media of LPS-stimulated Cal-27 cells and PD-L1 expression was measured by quantitative real-time PCR, western blotting and immunofluorescence. …”
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A systematic review of the transformation of Lake Beseka from brine to nearly fresh lake water by Yosef Abebe, Taye Alemayehu, Bizuneh Asfaw Abebe, Tena Alamirew, Beteley Teckola, Esayas Alemayehu

“…It is crucial to take into account options to lessen the effects of the adjacent Awash River by upgrading infrastructure and changing the lake's natural flow in order to address the issues with average water quantity. …”
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Application of the OMERACT Grey-scale Ultrasound Scoring System for salivary glands in a single-centre cohort of patients with suspected Sjögren’s syndrome by Lene Terslev, Simon Krabbe, Viktoria Fana, Uffe M Dohn

“…All underwent grey-scale ultrasound of the parotid and submandibular glands prior to clinical examination, Schirmer’s test, unstimulated salivary flow, blood samples including autoantibody analysis. …”
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Grouting Design of Rich Water Tunnels and the Calculation of Distance between Annular Blind Pipes by Helin Fu, Pengtao An, Kai Li, Guowen Cheng, Jie Li, Xiaohui Yu

“…On the other hand, the stratum grouting is used to control the discharge flow of groundwater. In the drainage system, it is important to determine the distance between the annular blind pipes, but there is no clear calculation formula, which leads to the designer often relying on experience. …”
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Long non-coding RNA NEAT1 promotes ovarian granulosa cell proliferation and cell cycle progression via the miR-29a-3p/IGF1 axis by Lina He, Jie Lin, Zhengwen Qin, Qing Xu, Li Hao, Yanhong Fu, Xu Ran, Wei Chen

“…The effects of NEAT1 overexpression or silencing on cell proliferation and cell cycle were evaluated using CCK-8 assays and flow cytometry. The interaction between NEAT1, miR-29a-3p, and IGF1 was examined using dual-luciferase reporter assays, qRT-PCR, and Western blot analysis. …”
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