Incorporation of recombinant proteins into extracellular vesicles by Lactococcus cremoris by Tina Vida Plavec, Kristina Žagar Soderžnik, Giulia Della Pelle, Špela Zupančič, Robert Vidmar, Aleš Berlec

“…The presence of vesicular structures (50–200 nm) in the samples was confirmed by transmission electron microscopy and by flow cytometry using membrane-specific stain. Mass spectrometry analyses confirmed the presence of recombinant proteins in the EVs fraction, with amounts ranging from 13.17 to 100%, highlighting their significant incorporation into the vesicles, together with intrinsic L. cremoris NZ9000 proteins that were either more abundant in the cytoplasm (ribosomal proteins, metabolic enzymes) or present in the membrane. …”
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Dysregulated T Cell Activation and Aberrant Cytokine Expression Profile in Systemic Lupus Erythematosus by Haiyan Zhou, Bojiang Li, Jing Li, Tongqian Wu, Xiaoqian Jin, Rui Yuan, Ping Shi, Yan Zhou, Long Li, Fang Yu

“…The expression of HLA-DR and costimulatory molecules on T cells was evaluated by flow cytometry. Concentrations of serum C-reactive protein, erythrocyte sedimentation rate, anti-double-stranded DNA (anti-dsDNA) antibody, total lgG, complement 3, and complement 4 were measured. …”
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Restoration of Sestrin 3 Expression Mitigates Cardiac Oxidative Damage in Ischemia–Reperfusion Injury Model by Mina Park, Sunghye Cho, Dongtak Jeong

“…Cardiac ischemia–reperfusion injury (IRI) occurs when blood flow is restored to the myocardium after a period of ischemia, leading to oxidative stress and subsequent myocardial cell damage, primarily due to the accumulation of reactive oxygen species (ROS). …”
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Effects of Ginsenoside Rg1 Regulating Wnt/β-Catenin Signaling on Neural Stem Cells to Delay Brain Senescence by Yue Xiang, Shun-he Wang, Lu Wang, Zi-ling Wang, Hui Yao, Lin-bo Chen, Ya-ping Wang

“…The following indicators were detected: (1) NSC culture identification, (2) the effect of LiCl on the proliferation and survival rate of NSCs, (3) the effect of ginsenoside Rg1 on the proliferation and survival of NSCs, (4) the growth of NSCs in each group observed by an optical microscope, (5) the cell cycle of each group detected by flow cytometry, (6) the proliferative ability of each group detected by BrdU, (7) the fluorescence intensity of Nestin and Sox2 of NSCs in each group observed by a fluorescence microscope, (8) the positive rate of senescence staining analyzed by SA-β-Gal staining, (9) the localization of β-catenin in NSCs observed by laser confocal microscopy, and (10) the changes of the Wnt/β-catenin pathway-related proteins in each group detected by Western blotting. …”
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CD3+CD8+CD28− T Lymphocytes in Patients with Lupus Nephritis by Marcelina Żabińska, Magdalena Krajewska, Katarzyna Kościelska-Kasprzak, Marian Klinger

“…We determined absolute count of CD3+CD8+CD28- and CD3+CD8+CD28-Foxp3+ subpopulations by flow cytometry. We observed a statistically significant increase in absolute count and percentage of CD3+CD8+CD28- in SLE patients compared to HC (p<0.001). …”
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Situation of Pediatric Patients with Testicular Torsion in Times of COVID-19 by Marta Komarowska, Małgorzata Kowalska, Kamil Grubczak, Alicja Pawelczyk, Adam Hermanowicz, Wojciech Debek, Ewa Matuszczak

“…In the 2019 group, Doppler ultrasound showed absent blood flow in the affected testicle in 62% vs. 80% in 2020. …”
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Erythropoietin Activates Autophagy to Regulate Apoptosis and Angiogenesis of Periodontal Ligament Stem Cells via the Akt/ERK1/2/BAD Signaling Pathway under Inflammatory Microenviro... by Denghao Huang, Jie Lei, Xingrui Li, Zhonghao Jiang, Maoxuan Luo, Yao Xiao

“…Annexin V-PI-FITC was used for cell apoptosis through flow cytometry. Matrigel plug was adopted to measure the angiogenic capacity in vitro. …”
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The Long Noncoding RNA LOXL1-AS1 Promotes the Proliferation, Migration, and Invasion in Hepatocellular Carcinoma by Jiang Liu, Chengtong Zhai, Degan Liu, Jianhua Liu

“…MTT assay, colony formation assay, flow cytometry analysis, transwell assays, and lentivirus-mediated RNA interference (RNAi) technology were used to evaluate cell proliferation and migration. …”
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Design and fabrication of novel microfluidic-based droplets for drug screening on a chronic myeloid leukemia cell line. by Niloofar Jaafari, Amir Asri Kojabad, Rima Manafi Shabestari, Majid Safa

“…Apoptosis was measured using Annexin/PI staining by flow cytometry.<h4>Results</h4>Significant increased apoptosis was seen in K562 cells treated with imatinib in the microfluidic device compared to cells treated with imatinib in 24- and 96-well plates. …”
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The Possible Role of Neutrophils in the Induction of Osteoclastogenesis by Carolyn G. J. Moonen, Teun J. de Vries, Patrick Rijkschroeff, Patrice E. Poubelle, Elena A. Nicu, Bruno G. Loos

“…RANKL expression on stimulated cPMNs, as assessed by flow cytometry and immunohistochemistry, was limited (6.48±0.72%, mean expression±SEM) after 24 and 48 hours of stimulation with LPS. …”
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Ecology through the eyes of a non-ecologist by Janusz Uchmański

“…In ecology, however, there is an alternative way to describe the dynamics of ecological systems, i.e. through the circulation of matter in them and the flow of energy through them. It allows the use of traditional difference and differential equations in the formulation of mathematical models, which has proven itself in practical applications many times.…”
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Study protocol for an observational cohort study of heat stress impacts in pregnancy in The Gambia, West Africa [version 2; peer review: 1 approved, 2 approved with reservations] by Jane E Hirst, Ana Bonell, Abdul Sesay, Leonidas G. Ioannou, Bubacarr Bah, Kris A. Murray, Andy Haines, Sophie E. Moore, David Jeffries, Neil S Maxwell, Ana Vicero-Cabrera, Apolline Saucy, Cally Tan, Emmanuel Okoh, Dorothy Watters, Bakary Sonko, Williams Oluwatosin Adefila, Yahaya Idris, Mam Leigh-Nabou, Jarra Manneh, Andreas Flouris, Sainabou Bojang, Amanda N Sferruzzi-Perri, Andrew Prentice

“…At the end of the week, an ultrasound scan will be performed to evaluate fetal size and placental blood flow. At delivery, birth outcomes will be recorded and maternal, placental and cord samples taken for epigenetic, biochemical and histological evaluation. …”
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To label or not? A choice experiment testing whether labelled green bonds matter to retail investors by Vasundhara Saravade, Olaf Weber, Adam Vitalis

“…Abstract Green bonds are an important sustainable finance tool that can help reorient financial flows and influence public policy in addressing climate action across the global financial markets. …”
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MicroRNA-541-3p/Rac2 signaling bridges radiation-induced lung injury and repair by Jiandong Zhang, Lei Ma, Limin He, Quanxiao Xu, Yan Ding, Lidong Wang

“…Methods: Mouse alveolar epithelial cells were transfected with a vector expressing microRNA-541-3p to regulate expression of target genes. Flow cytometry, polymerase chain reaction, and western blotting were used to analyze cell necroptosis, target gene expression, and target protein expression, respectively. …”
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New Leakage Model for Naturally Fractured Formations Considering the Effects of Dual-System Hydro-Mechanical Coupling by Hao Peng, Qian Li, JiaJia Gao, Hu Yin, Jun Wang

“…The dual-system between the formation and fracture is controlled by the flowing net pressure inside the fracture, which determines the dynamic width of the natural fracture and leak-off velocity. …”
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Pharmacological Inhibition of Glutaminase 1 Normalized the Metabolic State and CD4+ T Cell Response in Sjogren’s Syndrome by Jiayao Fu, Yiping Pu, Baoli Wang, Hui Li, Xiujuan Yang, Lisong Xie, Huan Shi, Zhijun Wang, Junhao Yin, Tianle Zhan, Yanxiong Shao, Changyu Chen, Qi Luo, Jiabao Xu, Zirui Zong, Xindi Wei, Wanwen Xiao, Chuangqi Yu, Lingyan Zheng

“…In vivo, we injected different doses of BPTES into SS-like NOD/Ltj mice and found that 10 mg/kg BPTES significantly restored the salivary flow rate. Histological and qRT–PCR analyses showed that this concentration of BPTES attenuated lymphocytic infiltration and the numbers of PCNA-positive cells and CD4+ T cells. …”
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Carotid-cavernous fistula as an ophthalmic disease requiring interdisciplinary treatment by Wojciech Luboń, Małgorzata Luboń, Ewa Mrukwa-Kominek

“…Doppler US showed dilated superior ophthalmic vein and increased arterialized vascular flow. Due to a strong suspicion of CCF, the patient was referred to the Department of Radiology for digital subtraction angiography (DSA). …”
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YTHDF2 mediates the protective effects of MG53 on myocardial infarction injury via recognizing the m6A modification of MG53 by Zhaojie Li, Kai Li, Jianqiang Zhao

“…Cell apoptosis was assessed by flow cytometry and TUNEL assay. Results The m6A levels of MG53 were increased in H/R-induced H9c2 cells and the myocardium of I/R rats. …”
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Patterns of Diversity of the Rissoidae (Mollusca: Gastropoda) in the Atlantic and the Mediterranean Region by Sérgio P. Ávila, Jeroen Goud, António M. de Frias Martins

“…The results of the analysis of the probable directions of faunal flows support the patterns found by both the Parsimony Analysis of Endemicity and the geographical distribution. …”
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What is the influence of single-entry models on access to elective surgical procedures? A systematic review by Deborah A Marshall, Zaheed Damani, Barbara Conner-Spady, Tina Nash, Henry Tom Stelfox, Tom W Noseworthy

“…Background Single-entry models (SEMs) for the management of patients awaiting elective surgical services are designed to increase access and flow through the system of care. We assessed scope of use and influence of SEMs on access (waiting times/throughput) and patient-centredness (patient/provider acceptability).Methods Systematic review of articles published in 6 relevant electronic databases included studies from database inception to July 2016. …”
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