Generation of IgM+ B cell-deficient Atlantic salmon (Salmo salar) by CRISPR/Cas9-mediated IgM knockout by Mari Raudstein, Ma. Michelle D. Peñaranda, Erik Kjærner-Semb, Søren Grove, H. Craig Morton, Rolf Brudvik Edvardsen

“…Gene expression analyses demonstrated significantly reduced membrane-bound IgM mRNA levels in head kidney and spleen tissues. Flow cytometry revealed a 78% reduction in IgM+ B cells in peripheral blood, and Western blot analyses showed decreased IgM protein levels in serum. …”
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Relationship between Corneal Temperature and Intraocular Pressure in Healthy Individuals: A Clinical Thermographic Analysis by Claudia Fabiani, Roberto Li Voti, Dario Rusciano, Maria Giulia Mutolo, Nicola Pescosolido

“…It can be speculated that both oxidative stress and sympathetic nerve fiber stimulation by temperature oscillations may affect the regulation of AH vortex flow and turnover, thus influencing IOP values.…”
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Circulating Interleukin-6 (but Not Other Immune Mediators) Associates with Criteria for Fried’s Frailty among Very Old Adults by Gilberto Santos Morais Junior, Diego Ignacio Valenzuela Perez, Audrey Cecília Tonet-Furioso, Lucy Gomes, Karla Helena Coelho Vilaça, Vicente Paulo Alves, Clayton Franco Moraes, Otávio Toledo Nóbrega

“…Clinical and biochemical data along with physical and cognitive assessments encompassing dual-energy X-ray scans and hand dynamometry were adopted to investigate frailty criteria, while circulating immune mediators (IFNγ, IL-2, IL-4, IL-6, IL-10, and TNFα) were assessed using high-throughput flow cytometry. Results. Preliminarily, IL-6 correlated positively with waist-to-hip ratio and C-reactive protein and negatively with glycemia. …”
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UHPLC-UV method validation for simultaneous quantification of vitexin and isovitexin from Santalum album L. leaves by Thi Kim Phung Phan, Minh Quan Le, Kien Duc Ngo

“…The quantification procedure was performed on a Hypersil GOLD aQ Column (3 μm; 150 × 2.1 mm) at 35°C, with a mobile phase of acetonitrile (A) and and water with 0.1% formic acid (B), a flow rate of 0.3 mL/min, a detection wavelength of 336 nm, and an injection volume of 3 µL. …”
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Multi-faceted examination of a deepwater seamount reveals ecological patterns among coral and sponge communities in the equatorial Pacific by Brian RC Kennedy, Steven Auscavitch, Timothy M. Shank, Constance Sartor, Anameere Tennaba, Alexis M. Weinnig, Randi D. Rotjan

“…To help visualize the influence of current flow, oxygen, depth, and substrate type on the zonation of seamounts, we created an Alexander Von Humboldt-style infographic to illustrate the observed biodiversity patterns. …”
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Complement C5a Alters the Membrane Potential of Neutrophils during Hemorrhagic Shock by David A. C. Messerer, Stephanie Denk, Karl J. Föhr, Rebecca Halbgebauer, Christian K. Braun, Felix Hönes, Julia Harant, Michael Fauler, Manfred Frick, Benedikt L. Nußbaum, Peter Radermacher, Sebastian Hafner, Markus S. Huber-Lang

“…Electrophysiological changes in cellular and mitochondrial membrane potential and intracellular pH of PMN from human healthy volunteers were determined by flow cytometry after exposure to C5a. Furthermore, PMN from male Bretoncelles-Meishan-Willebrand cross-bred pigs before and three hours after severe hemorrhagic shock were analyzed for their electrophysiological response. …”
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Qufeng epimedium decoction alleviates rheumatoid arthritis through CYLD-antagonized NF-kB activation by deubiquitinating Sirt1 by Zhiming Wu, Peng Zhang, Wenyan Huang, Yifen Zhou, Zhengliu Cao, Chunhong Wu

“…Methods: The RA animal model was established to investigate the anti-RA effect and regulatory effect on fibroblast-like synoviocytes (FLS) pyroptosis, qRT-PCR, Western blot, flow cytometry, histology staining, and ELISA were utilized to confirm the gene and protein expressions. …”
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Bat Cave Vulnerability Index 3.0 (BCVI-S): An integrative and scalable tool to prioritise bat caves for conservation by Krizler C. Tanalgo, Alice Catherine Hughes

“…Given the challenge of assessing many taxa, bats are ideal ecological indicators of caves and subterranean ecosystems, because they serve as keystone species that facilitate energy flow to other life forms inside caves. We calibrated the Bat Cave Vulnerability Index 3.0 (BCVI-S) and its indicators to optimise the scalability and applicability of the index in wider contexts by improving the calculations of Biotic Potential and the use of more standardised remotely sensed data to obtain Biotic Vulnerability based on landscape features. …”
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Evaluation of the Effect of Quince Seed Extract On Physical and Sensorial Properties of Gluten-Free Cake Batter Formulations by Sinem Argun, Ceren Cokeker, Emrah Kırtıl

“…All cake batters displayed a pseudoplastic flow behavior with apparent viscosities and shear thinning behavior increasing substantially with increasing QSE concentrations. …”
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Circulating Th17, Th22, and Th1 Cells Are Elevated in the Guillain-Barré Syndrome and Downregulated by IVIg Treatments by Shujuan Li, Tao Jin, Hong-Liang Zhang, Hong Yu, Fanhua Meng, Hernan Concha Quezada, Jie Zhu

“…Here, we detected the frequency of Th1, Th17, and Th22 cells by using 4-color flow cytometry and we detected the plasma levels of IL-17 and IL-22 by ELISA in GBS patients, relapsing-remitting multiple sclerosis patients at the acute phase of relapse, viral encephalitis or meningitis patients and healthy controls. …”
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Effects of radiation and manganese oxide nanoparticles on human glioblastoma cell line U-87 MG glycolysis by N. B. Illarionova, D. V. Petrovski, I. A. Razumov, E. L. Zavyalov

“…Their count was performed using flow cytometry. We showed that the glycolysis of U-87 MG glioma cells was significantly reduced when incubated for 48 hours with manganese oxide NPs. …”
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Increased Death of Peripheral Blood Mononuclear Cells after TLR4 Inhibition in Sepsis Is Not via TNF/TNF Receptor-Mediated Apoptotic Pathway by Chien-Ming Chu, Li-Chung Chiu, Chung-Chieh Yu, Li-Pang Chuang, Kuo-Chin Kao, Li-Fu Li, Huang-Pin Wu

“…The cell death of PBMCs was detected using a flow cytofluorimeter. Results. After TLR4 inhibition, Bcl2 to Bax ratio elevated both in LPS and HMGB1-stimulated PBMCs. …”
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Analysis of the Impact of the Russia–Ukraine Conflict on Global Liquefied Natural Gas Shipping Network by Ranxuan Ke, Xiaoran Wang, Peng Peng

“…The research indicated that: (1) The alteration in trade relations instigated by the Russia–Ukraine conflict modified global LNG flows, resulting in a fragmented overall network structure and diminished transportation efficiency. …”
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Numerical investigation of segmental baffle design in shell and tube heat exchangers with varying inclination angles and spacing by A. M. Mohammadzadeh, Bahram Jafari, Khashayar Hosseinzadeh, E. Paikar

“…This research uses a three-dimensional computational fluid dynamics (CFD) simulation to model turbulent fluid flow and evaluate the performance of different baffle angle configurations. …”
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Forward model emulator for atmospheric radiative transfer using Gaussian processes and cross validation by O. Lamminpää, J. Susiluoto, J. Hobbs, J. McDuffie, A. Braverman, H. Owhadi

“…Our approach is based on Gaussian process (GP) regression, leveraging recent research on kernel flows and cross validation to efficiently learn the kernel function in the GP. …”
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A sustainable and reliable model for iron closed-loop supply chain network design with considering risk and lateral transmision and developed multi-objective decision-making method by Hamid Saffari, Morteza Abbasi, Jafar Gheidar-Kheljani

“…Also, the amount of cost required for allocation to reliable and unreliable facilities and also creating a suitable Pareto space for deciding on the optimal choice of facilities, capacity and flow between them and iron and steel production technology, according to sustainability and social responsibility indicators, are other research findings.Originality/Value: In this study, for the first time, the design of a robust, sustainable, and resilient network of iron and steel under different risks has been studied. …”
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Two-colour QCD phases and the topology at low temperature and high density by Kei Iida, Etsuko Itou, Tong-Gyu Lee

“…Finally, at temperature of order 0.45Tc, where Tc is the chiral transition temperature at zero chemical potential, the topological susceptibility is calculated from a gradient-flow method and found to be almost constant for all the values of μ ranging from the hadronic to BCS phase. …”
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Statin drugs enhance responses to immune checkpoint blockade in head and neck cancer models by Chrystal Paulos, Gregory B Lesinski, Zachary S Buchwald, Nabil F Saba, Vikash Kansal, Andre J Burnham, Brendan L C Kinney, Nicole C Schmitt

“…In mice, daily oral simvastatin or lovastatin enhanced tumor control and extended survival when combined with PD-1 blockade, with rejection of MOC1 tumors in 30% of mice treated with lovastatin plus anti-PD-1. Results from flow cytometry of tumors and tumor-draining lymph nodes suggested T cell activation and shifts from M2 to M1 macrophage predominance as potential mechanisms of combination therapy.Conclusions These results suggest that statins deserve further study as well-tolerated, inexpensive drugs that may enhance responses to PD-1 checkpoint blockade and other immunotherapies for HNSCC.…”
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Simulation Results of a Thermal Power Dispatch System from a Generic Pressurized Water Reactor in Normal and Abnormal Operating Conditions by Stephen Hancock, Dylan Jurski, Thomas A. Ulrich, Roger Lew, Jisuk Kim, Olugbenga Gideon

“…Standard malfunctions were included in the simulation design, including steam line breaks at various system locations and flow interruptions in the hydrogen plant due to multiple faults, reflecting anticipated operational challenges. …”
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Improving paraffin precipitate inhibition using glycine and Palm-based Methyl Ester Sulfonate (MES) eco-friendly inhibitors. by Nazliah Binti Surpina, Mysara Eissa Mohyaldinn, Abdullah Abduljabbar, Mohammed Abdalla Ayoub

“…While PPT results were insignificant, MES is recommended as a flow improver rather than a pour point depressant. …”
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