Role of hepatocyte RIPK1 in maintaining liver homeostasis during metabolic challenges by Weigao Zhang, Hu Liu, Danyang Zhang, Yuguo Yi, Liang Tao, Yunfeng Zhu, Shuxian Huang, Xunan Zhao, Qianchao Shao, Peiqi Li, Yiwen Weng, Wei Lu, Jianfa Zhang, Haibing Zhang, Yuxin Chen, Dan Weng

“…Transcriptomic analysis revealed that short-term fasting oriented the hepatic microenvironment into an inflammatory state in Ripk1-hepKO mice, with up-regulated expression of inflammation and immune cell recruitment-associated genes. …”
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Targeting NANOS1 in triple-negative breast cancer: synergistic effects of digoxin and PD-1 inhibitors in modulating the tumor immune microenvironment by Tangyi Wang, Yadian Lei, Jingwei Sun, Li Wang, Yuxin Lin, Zhijing Wu, Shoude Zhang, Chengzhu Cao, Chengzhu Cao, Chengzhu Cao, Haiyan Wang, Haiyan Wang, Haiyan Wang

“…Survival analysis showed lower NANOS1 expression correlated with better prognosis. Immunoinfiltration analysis indicated a negative correlation between NANOS1 levels and activated NK cells. …”
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Novel role of FTO in regulation of gut–brain communication via Desulfovibrio fairfieldensis-produced hydrogen sulfide under arsenic exposure by Ruonan Chen, Xiaoqin Chai, Yunxiao Zhang, Tianxiu Zhou, Yinyin Xia, Xuejun Jiang, Bo Lv, Jun Zhang, Lixiao Zhou, Xin Tian, Ruonan Wang, Lejiao Mao, Feng Zhao, Hongyang Zhang, Jun Hu, Jingfu Qiu, Zhen Zou, Chengzhi Chen

“…Also, this administration resulted in the enhancement of m6A modification and the reduction of FTO expression in the intestine. By using both CRISPR/Cas9-based FTO knock-in strategy and adeno-associated virus (AAV)-mediated overexpression of FTO in the intestine, we established for the first time that up-regulation of FTO remarkably ameliorated arsenic-induced disruption of intestinal barriers and altered microenvironment of mice. …”
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Wild grasses as the reservoirs of infection of rust species for winter soft wheat in the Northern CaucasusWild grasses as the reservoirs of infection of rust species for winter sof... by E. I. Gultyaeva, L. A. Bespalova, I. B. Ablova, E. L. Shaydayuk, Zh. N. Khudokormova, D. R. Yakovleva, Yu. A. Titova

“…Depending on weather conditions, the symptom expression is noted from late September to December and then from late February to May–June. …”
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Soluble and cell-based markers of immune checkpoint inhibitor-associated nephritis by Sandra M Herrmann, Riley Fadden, Ryan J Sullivan, Kerry L Reynolds, Harish Seethapathy, Meghan E Sise, Shruti Gupta, Alexandra-Chloe Villani, Qiyu Wang, Daiana Moreno, Destiny Harden, R Neal Smith, Ivy A Rosales, Robert B Colvin, Sarah Chute, Lynn D Cornell, Nancy J Yang, Sara Barmettler, Sophia Wells, Jocelyn Farmer

“…By peripheral blood flow cytometry analysis, lower absolute CD8+T cells, CD45RA+CD8+ T cells, memory CD27+B cells, and expansion of plasmablasts were prominent features of ICI-nephritis compared with ICI-treated controls. Gene expressions for IL2RA, IL-2 signaling, and T cell receptor signaling in the kidney tissue with ICI-nephritis were significantly higher compared with controls.Conclusion Elevated sIL-2R level and flow cytometric markers of both B and T cell dysregulation may aid the diagnosis of ICI-nephritis.…”
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Transcriptomic Profiling and Tumor Microenvironment Classification Reveal Unique and Dynamic Immune Biology in HIV-Associated Kaposi Sarcoma by Jihua Yang, Ayse Ece Cali Daylan, Aleksei Shevkoplias, Ekaterina Postovalova, Meng Wang, Andrey Tyshevich, Matthew Lee, Hiba Narvel, Ksenia Zornikova, Nara Shin, Nikita Kotlov, Luca Paoluzzi, Changcheng Zhu, Balazs Halmos, Xingxing Zang, Haiying Cheng

“…Notably, HIV-negative KS samples showed alterations in NK cell-mediated immunity and cytotoxic response pathways, whereas HIV-positive samples exhibited changes in growth regulation and protein kinase activity pathways at the time of initial diagnosis. The gene expression of immune checkpoints, including CD274 (PD-L1) and PDCD1LC2 (PD-L2), was comparable between HIV-positive and HIV-negative KS samples at diagnosis. …”
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Maternal exposure to deltamethrin during pregnancy and lactation impairs hippocampal learning and memory function of male offspring by ferroptosis by Shasha Huang, Wanqi Li, Dengke Wang, Huiwen Feng, Bo Wang, Xinyu Dong, Wei Zhao, Dan Liu, Yuan Wang

“…Moreover, maternal exposure to DM increased the expression of ferrous ion, malondialdehyde (MDA) and prostaglandin-endoperoxide synthase 2 (PTGS2) protein, and decreased the glutathione (GSH) level in the hippocampus, which was contributed to ferroptosis by p53-mediated solute carrier family 7 member 11 (SLC7A11)/glutathione peroxidase 4 (GPX4) axis in the male offspring. …”
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Immunogenicity and safety of a live-attenuated SARS-CoV-2 vaccine candidate based on multiple attenuation mechanisms by Mie Suzuki Okutani, Shinya Okamura, Tang Gis, Hitomi Sasaki, Suni Lee, Akiho Kashiwabara, Simon Goto, Mai Matsumoto, Mayuko Yamawaki, Toshiaki Miyazaki, Tatsuya Nakagawa, Masahito Ikawa, Wataru Kamitani, Shiro Takekawa, Koichi Yamanishi, Hirotaka Ebina

“…Furthermore, we confirmed the safety of BK2102 using transgenic (Tg) mice expressing human ACE2 (hACE2) that are highly susceptible to SARS-CoV-2. …”
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Phenotype of Peripheral NK Cells in Latent, Active, and Meningeal Tuberculosis by José Alberto Choreño-Parra, Luis Armando Jiménez-Álvarez, Ellis Daniela Maldonado-Díaz, Graciela Cárdenas, Luis Alejandro Fernández-Lopez, José Luis Soto-Hernandez, Marcela Muñoz-Torrico, Gustavo Ramírez-Martínez, Alfredo Cruz-Lagunas, Armando Vega-López, María Lilia Domínguez-López, Carlos Sánchez-Garibay, Parménides Guadarrama-Ortíz, Silvia Giono, Luis Antonio Jiménez-Zamudio, Shabaana A. Khader, Ethel A. García-Latorre, Citlaltepetl Salinas-Lara, Joaquín Zúñiga

“…We found that NK cells from TBM patients showed lower absolute frequencies, higher CD69 expression, and poor expansion of the CD45RO+ memory-like subpopulation upon Mtb exposure in vitro compared to LTBI individuals. …”
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Exosomal mir-126-3p derived from endothelial cells induces ion channel dysfunction by targeting RGS3 signaling in cardiomyocytes: a novel mechanism in Takotsubo cardiomyopathy by Xuehui Fan, Guoqiang Yang, Yinuo Wang, Haojie Shi, Katja Nitschke, Katherine Sattler, Mohammad Abumayyaleh, Lukas Cyganek, Philipp Nuhn, Thomas Worst, Bin Liao, Gergana Dobreva, Daniel Duerschmied, Xiaobo Zhou, Ibrahim El-Battrawy, Ibrahim Akin

“…Western blot and qPCR analyses confirmed that miR-126-3p-mimic reduced RGS3 expression in both HCMECs and hiPSC-CMs, indicating miR-126-3p inhibits RGS3 signaling. …”
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Platelet‐derived apoptotic vesicles ameliorate nonalcoholic fatty liver disease by regulating lipid metabolism via apolipoprotein A‐II by Yuhe Jiang, Yike Liao, Zeying Wang, Lei Zhu, Yunsong Liu, Ping Zhang, Yuan Zhu, Wenyue Li, Yongsheng Zhou, Xiao Zhang

“…Owing to the higher expression of APOA2, PLT‐apoVs showed better therapeutic effects than MSC‐apoVs. …”
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Effect of the gut core microbiota Cetobacterium on the growth, physiology, and nutritional metabolism of Nile tilapia (Oreochromis niloticus) by Miao Wang, Chenglin Tang, Ziyue Zhang, Zijian Fan, Lijin Jiang, Zhigang Liu, Jianmeng Cao, Zhang Wang, Maixin Lu, Mengmeng Yi, Xiaoli Ke

“…Furthermore, analysis of the liver transcriptome and RT-qPCR data indicated a decrease of genes expression of fatty acid synthesis (SREB 1c, ACCα, and FAS) with NK01 supplementation feeding group. …”
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Osteopenia Metabolomic Biomarkers for Early Warning of Osteoporosis by Jie Wang, Dandan Yan, Suna Wang, Aihua Zhao, Xuhong Hou, Xiaojiao Zheng, Jingyi Guo, Li Shen, Yuqian Bao, Wei Jia, Xiangtian Yu, Cheng Hu, Zhenlin Zhang

“…We conducted individual edge network analysis (iENA) combined with a random forest to detect metabolomic biomarkers for the early warning of osteoporosis. Weighted Gene Co-Expression Network Analysis (WGCNA) and mediation analysis were used to explore the clinical impacts of metabolomic biomarkers. …”
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Varroa Volatiles Offer Chemical Cues to Honey Bees for Initial Parasitic Recognition by Qinglong Zhao, Xinning Wang, Ahsan Mustafa, Ying Wang, Hongfang Wang, Xuepeng Chi, Baohua Xu, Zhenguo Liu

“…After exposure to ethyl palmitate for 1 h, the relative expression levels of <i>AcerCSP1</i> and <i>AcerOBP21</i> in <i>A. c. cerana</i> significantly increased, as well as the level of <i>AmelCSP1</i> in <i>A. m. ligustica</i>, while <i>AmelOBP8</i> showed no significant changes. …”
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Pathogenic variants of TUBB8 cause oocyte spindle defects by disrupting with EB1/CAKP5 interactions and potential treatment targeting microtubule acetylation through HDAC6 inhibiti... by Hui Luo, Jianhua Chen, Cao Li, Tian Wu, Siyue Yin, Guangping Yang, Yipin Wang, Zhihan Guo, Saifei Hu, Yanni He, Yingnan Wang, Yao Chen, Youqiang Su, Congxiu Miao, Yun Qian, Ruizhi Feng

“…Live‐cell imaging and immunofluorescence were utilised to track the dynamic expression of microtubule plus end‐tracking proteins in vivo and analysed microtubule nucleation or spindle assembly in vitro, respectively. …”
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Efficacy of first-line chemotherapy combined with immunotherapy or anti-angiogenic therapy in advanced KRAS-mutant non-small cell lung cancer by Huiping Qiang, Yue Wang, Yao Zhang, Jingwen Li, Lincheng Zhang, Huawei Du, Xuxinyi Ling, Shuhui Cao, Yan Zhou, Runbo Zhong, Hua Zhong

“…Their clinical baseline characteristics, first-line treatment strategy, whether combined TP53 or STK11 mutation, PD-L1 expression level, etc. were evaluated. The correlation between these factors and progression-free survival (PFS) and overall survival (OS) were analyzed. …”
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Neoantigen-specific stimulation of tumor-infiltrating lymphocytes enables effective TCR isolation and expansion while preserving stem-like memory phenotypes by Yong Li, Steven A Rosenberg, Paul F Robbins, Zhiya Yu, Maria Parkhurst, Billel Gasmi, Nikolaos Zacharakis, Todd D Prickett, Jared J Gartner, Sri Krishna, Stephanie L Goff, Satyajit Ray, Frank J Lowery, Sivasish Sindiri, Alakesh Bera, Noam Levin, Sanghyun P Kim, Charles A Marquardt, Nolan R Vale, Lior Levy, Tiffany Benzine, Robert V Masi, Rafiqul Islam

“…Single-cell transcriptome analysis revealed that neoantigenic stimulation increased neoantigen-reactive TILs with stem-like memory phenotypes expressing IL-7R, CD62L, and KLF2. Furthermore, neoantigenic stimulation improved the in vivo antitumor efficacy of TILs relative to the conventional OKT3-induced rapid TIL expansion in p53-mutated or KRAS-mutated xenograft mouse models.Conclusions Taken together, neoantigenic stimulation of TILs selectively expands neoantigen-reactive TILs by frequencies and by their clonal repertoire. …”
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Exploring the potential of an oyster (Saccostrea mordax) in oligotrophic seas: Effects of starvation on physiological and molecular regulation by Shuming Guo, Liqiang Zhao, Yuexin Zhao, Zohaib Noor, Xiaogang Yang, Shiwei Fu, Zhen Zhao, Weitao Wan, Jinkuan Wei, Yanping Qin, Haitao Ma, Ziniu Yu, Jun Li, Yuehuan Zhang

“…The integration of transcriptomics analyses indicates that the metabolic capacity of oysters decreases under prolonged starvation, and the expression of genes in the solute carrier family that contribute to nutrient transport was down-regulated. …”
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Automated multidimensional deep learning platform for referable diabetic retinopathy detection: a multicentre, retrospective study by Chi Pui Pang, Weiqi Chen, Mingzhi Zhang, Tsz Kin Ng, Yi Zheng, Guihua Zhang, Jian-Wei Lin, Ji Wang, Jie Ji, Ling-Ping Cen, Peiwen Xie, Yongqun Xiong, Hanfu Wu, Dongjie Li

“…The performance of the DL was compared with DR experts.Setting DR screening programmes from three hospitals and the Lifeline Express Diabetic Retinopathy Screening Program in China.Participants 83 465 images of 39 836 eyes from 21 716 patients were annotated, of which 53 211 images were used as the development set and 30 254 images were used as the external validation set, split based on centre and period.Main outcomes Accuracy, F1 score, sensitivity, specificity, area under the receiver operating characteristic curve (AUROC), area under the precision-recall curve (AUPRC), Cohen’s unweighted κ and Gwet’s AC1 were calculated to evaluate the performance of the DL algorithm.Results In the external validation set, the five classifiers achieved an accuracy of 0.915–0.980, F1 score of 0.682–0.966, sensitivity of 0.917–0.978, specificity of 0.907–0.981, AUROC of 0.9639–0.9944 and AUPRC of 0.7504–0.9949. …”
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Implementasi Metode Naive Bayes untuk Mendeteksi Stres Siswa Berdasarkan Tweet pada Sistem Monitoring Stres by Diva Fardiana Risa, Fajar Pradana, Fitra Abdurrachman Bachtiar

“…When a person experiences anxiety disorders, especially stress, he will tend to take actions that can express his anxiety on social media. This activity is called Self Disclosure. …”
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