Student Engagement and Feedback on Online Learning and Facilitation: A Case Study of the National Open University of Nigeria by Adewale Adesina, Dorothy Ofoha, Olufemi A. Peters, Isaac S. Butswat

“…A majority of students (61.9%) reported participating in the first semester of the 2023 facilitation sessions and expressed satisfaction with their online learning experience, but that more awareness and effective communication strategies are needed to enhance the process. …”
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β-synuclein in cerebrospinal fluid as a potential biomarker for distinguishing human prion diseases from Alzheimer’s and Parkinson’s disease by Bing Xu, Kang Xiao, Xiaoxi Jia, Rundong Cao, Donglin Liang, Ruhan A, Weiwei Zhang, Chunjie Li, Liping Gao, Cao Chen, Qi Shi, Xiaoping Dong

“…Abstract Background β-synuclein (β-syn), mainly expressed in central nerve system, is one of the biomarkers in cerebrospinal fluid (CSF) and blood for synaptic damage, which has been reported to be elevated in CSF and blood of the patients of prion diseases (PrDs). …”
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Proteomic analysis to explore potential mechanism underlying pseudomale sperm defect in Cynoglossus semilaevis by Xihong Li, Lu Li, Zhongkai Cui, Ming Li, Xuexue Sun, Zhijie Li, Zhangfan Chen, Lanqing Ding, Dongdong Xu, Wenteng Xu

“…Totally, 783 differentially expressed proteins (DEPs) including 495 up-regulated and 288 down-regulated proteins were identified in pseudomale group. …”
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Genes Induced by Panax Notoginseng in a Rodent Model of Ischemia-Reperfusion Injury by Lanqing Meng, Qing Huang, Xuebin Li, Ping Liang, Yueyong Li, Xiaohua Huang, Jingjie Zhao, Qiuping Chen, Rong Qiu, Lan Li, Chongdong Jian, Hongfei Yao, Jianmin Huang, Xionglin Tang, Zechen Wang, Zhongheng Wei, Jun Wu, Liuzhi Wei, Qiuju Wei, Qianli Tang, Lu Huang, Jihua Wei, Dinggui Lu, Qunqiang Luo, Kegong Xie, Yang Ouyang, Jian Chen, Genliang Li, Linxue Luo, Linbo He, Chenyi Zhuo, Anding Xu, Lingzhang Meng

“…RNA-Seq was performed to determine the differential expressed genes (DEGs) which were then verified by qPCR. …”
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Differentiation of high risk prostate cancer with a facile urinary exosome detection workflow by Shuai Qiu, Yue Li, Zheng Zhang, Chunchang Li, Haoyu Wang, Ao Chen, Zhi Yan, Yang Liu, Zifei Li, Hua Huang, Yi Liu, Yiqi Seow, Ruibing Chen, Jinhong Guo, Simeng Wen, Jing Tian, Hongtuan Zhang, Ranlu Liu, Gang Han, Baolong Wang, Yong Wang, Yuanjie Niu, HaiFang Yin

“…We discovered that EEF1A1 levels, abundantly expressed on urinary exosomes, positively correlate to urinary exosome counts irrespective of source and collection time and demonstrated that EEF1A1 enables in-assay quantification of urinary exosomes. …”
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SECTM1 acts as an immune-related biomarker of poor prognosis and promotes cancer progression by modulating M2 macrophage polarization in esophageal squamous cell carcinoma by Pengzhou Kong, Pengzhou Kong, Pengzhou Kong, Ye Jiao, Ye Jiao, Meng Sun, Meng Sun, Zhinan Zhou, Zhinan Zhou, Yingying Zhang, Yingying Zhang, Xin Yang, Xin Yang, Jing Ren, Jing Ren, Mengyuan Yang, Mengyuan Yang, Yanyan Dong, Yanyan Dong, Yanyan Dong, Bin Song

“…We established of two ESCC subtypes characterized by high and low immune profiles, and 352 differentially expressed immune genes were identified between the two subtypes. …”
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Paracrine activity of Smurf1-silenced mesenchymal stem cells enhances bone regeneration and reduces bone loss in postmenopausal osteoporosis by Alberto González-González, Itziar Álvarez-Iglesias, Daniel García-Sánchez, Monica Dotta, Ricardo Reyes, Ana Alfonso-Fernández, Alfonso Bolado-Carrancio, Patricia Díaz-Rodríguez, María Isabel Pérez-Núñez, José Carlos Rodríguez-Rey, Jesús Delgado-Calle, Flor M. Pérez-Campo

“…Additionally, in ex vivo human bone cultures, treatment with hCM-Smurf1 significantly reduced RANKL expression without affecting OPG levels, indicating an anti-resorptive effect. …”
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Klebsiella pneumoniae-derived extracellular vesicles impair endothelial function by inhibiting SIRT1 by Xinxin Li, Jinghua Cui, Zanbo Ding, Ziyan Tian, Yiming Kong, Linghai Li, Yang Liu, Wen Zhao, Xueying Chen, Han Guo, Zhengshuo Cui, Xinwei Li, Jing Yuan, Huina Zhang

“…Methods K.pn EVs were purified from the bacterial suspension using ultracentrifugation and characterized by transmission electron microscopy nanoparticle tracking analysis, and EV marker expression. Endothelium-dependent relaxation was measured using a wire myograph after in vivo or ex vivo treatment with K.pn EVs. …”
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Cerebral microvascular density, blood-brain barrier permeability, and support for neuroinflammation indicate early aging in a Marfan syndrome mouse model by Tala Curry-Koski, Tala Curry-Koski, Liam P. Curtin, Mitra Esfandiarei, Mitra Esfandiarei, Mitra Esfandiarei, Theresa Currier Thomas, Theresa Currier Thomas, Theresa Currier Thomas, Theresa Currier Thomas

“…Despite these significant clinical observations, investigation into cerebrovascular dysfunction and neuropathology in MFS remains limited.MethodsUsing 6M-control (C57BL/6) and 6M-MFS (Fbn1C1041G/+) and healthy 12M-control male and female mice, we test the hypothesis that abnormal Fbn1 protein expression is associated with altered cerebral microvascular density, BBB permeability, and neuroinflammation in the PCA-perfused hippocampus, all indicative of a pre-mature aging brain phenotype. …”
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Monitoring of environmental gases and calf health in Italian Mediterranean buffalo herd by Simona Leonetti, Monica Ambrosio, Mirko Scamardella, Chiara Caso, Angelo Coletta

“…Finally, as regards the state of cleanliness of the litter, rather than in the calf score, the state of cleanliness of the litters was considered in the data collection regarding the management of the calf house, expressing a judgment such as: “The litter is: kept in good condition; fairly well kept; it is not kept in good condition”. …”
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Prognostic Impact of Adjuvant Immunotherapy in Patients With Resectable NSCLC After Neoadjuvant Chemoimmunotherapy: A Brief Report by Yichen Dong, MD, Long Xu, MD, Jialiang Wen, MD, Haojie Si, MD, Juemin Yu, MD, Tao Chen, MD, Huikang Xie, MD, Xinjian Li, MD, Minglei Yang, MD, Junqiang Fan, MD, Junqi Wu, MD, Yunlang She, MD, Deping Zhao, MD, Chang Chen, MD, PhD

“…Subgroup analyses found that patients with a squamous histologic type, positive PD-L1 expression, and those with a major pathologic response particularly benefited from adjuvant immunotherapy. …”
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Transcriptomic signatures of cold acclimated adipocytes reveal CXCL12 as a Brown autocrine and paracrine chemokine by Marina Agueda-Oyarzabal, Marie S. Isidor, Kaja Plucińska, Lars R. Ingerslev, Oksana Dmytriyeva, Patricia S.S. Petersen, Sara Laftih, Axel B. Pontoppidan, Jo B. Henningsen, Kaja Rupar, Erin L. Brown, Thue W. Schwartz, Romain Barrès, Zachary Gerhart-Hines, Camilla C. Schéele, Brice Emanuelli

“…Among 858 cold-sensitive transcripts in BAT adipocytes were 210 secreted factor-encoding genes, and Cxcl12 was the top brown adipocyte-enriched cytokine. Cxcl12 mRNA expression analysis by RT-qPCR and fluorescence in situ hybridization specified Cxcl12 distribution in various cell types, and indicated its enrichment in cold-acclimated brown adipocytes. …”
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Blue elixir of youth and wellness: The multicomponent-multitarget paradigm of Curcuma caesia Roxb by Sabrina Burattini, Federico Gianfanti, Francesco Onesimo, Sabrina Donati Zeppa, Barbara Canonico, Mariele Montanari, Giovanna Panza, Daniele Lopez, Giosuè Annibalini, Fabiana Fanelli, Roberta Saltarelli, Michele Mari, Michele Retini, Riham Osman, Aghna Mariam, Matteo Micucci, Giovanni Caprioli, Agnese Santanatoglia, Deborah Agostini, Michela Battistelli

“…Additionally, CCRE decreased UVB-induced IL-6 expression and IKK phosphorylation, which play a crucial role in inflammaging. …”
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Intrinsic subtype and immunity score in identification of triple-negative breast cancer at low risk by Xinyu Ren, Tong Zhou, Yu Song, Huanwen Wu, Jeff Chou, Lance D. Miller, Zhiyong Liang, Songjie Shen

“…Intrinsic subtypes (basal-like, immune-enhanced, human epidermal growth factor receptor-2 [HER2]-enriched and luminal A/B) and tumor environmental immunity were evaluated using expression profiles of a 72-gene panel. Association of intrinsic subtype and immunity score with distant metastasis-free survival (DMFS) and overall survival (OS) was analyzed. …”
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SSL5-AnxA5 Fusion Protein Constructed Based on Human Atherosclerotic Plaque scRNA-Seq Data Preventing the Binding of Apoptotic Endothelial Cells, Platelets, and Inflammatory Cells by Yifei Zhao, Xingyu He, Teng Hu, Tianli Xia, Fangyang Huang, Changming Li, Yiming Li, Fei Chen, Mao Chen, Jun Ma, Yong Peng

“…<b>Methods:</b> The scRNA-seq data were extracted from the Gene Expression Omnibus (GEO) database. Single-cell transcriptome analysis results and cell–cell communication were analyzed to identify the ACS and SAP cell clusters and elucidate the intercellular communication differences. …”
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Evaluation of the cardiotoxicity of Echinochrome A using human induced pluripotent stem cell-derived cardiac organoids by Su-Jin Lee, Eunji Kim, Yeeun Jeong, Jae Boum Youm, Hyoung Kyu Kim, Jin Han, Elena A. Vasileva, Natalia P. Mishchenko, Sergey A. Fedoreyev, Valentin A. Stonik, Sung Joon Kim, Hyang-Ae Lee

“…The hCOs, characterized by expression of key cardiac markers (cTnT) and functional ion channels (Cav1.2, Nav1.5, hERG), exhibited structural and electrophysiological properties reflective of human cardiac physiology. …”
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Enhancement of Doxorubicin Efficacy by Bacopaside II in Triple-Negative Breast Cancer Cells by Sima Kianpour Rad, Kenny K. L. Yeo, Runhao Li, Fangmeinuo Wu, Saifei Liu, Saeed Nourmohammadi, William M. Murphy, Yoko Tomita, Timothy J. Price, Wendy V. Ingman, Amanda R. Townsend, Eric Smith

“…ATP-binding cassette (ABC) transporter expression (<i>ABCB1</i>, <i>ABCC1</i>, <i>ABCC3</i>, and <i>ABCG2</i>) was analyzed for correlations with drug sensitivity. …”
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The AMD-associated genetic polymorphism CFH Y402H confers vulnerability to Hydroquinone-induced stress in iPSC-RPE cells by Angela Armento, Inga Sonntag, Ana-Cristina Almansa-Garcia, Merve Sen, Sylvia Bolz, Blanca Arango-Gonzalez, Ellen Kilger, Ruchi Sharma, Kapil Bharti, Rosario Fernandez-Godino, Berta de la Cerda, Simon J. Clark, Simon J. Clark, Simon J. Clark, Marius Ueffing

“…There has been, and continues to be, much discussion around the functional consequences of this Y402H polymorphism, whether the soluble FH protein confers its risk association, or if the cells expressing the protein themselves are affected by the genetic alteration. …”
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The SERPINB4 gene mutation identified in twin patients with Crohn’s disease impaires the intestinal epithelial cell functions by Xiao-Mei Ouyang, Jun-Hui Lin, Ying Lin, Xian-Ling Zhao, Ya‐Ni Huo, Lai-Ying Liang, Yong-Dong Huang, Gui-Jing Xie, Peng Mi, Zhen-Yu Ye, Bayasi Guleng

“…Transcriptome sequencing revealed that the expression of genes encoding proinflammatory proteins (IL1B, IL6, IL17, IL24, CCL2, and CXCR2) and key proteins in the immune response (S100A9, MMP3, and MYC) was significantly upregulated during SERPINB4 mutant-induced apoptosis. …”
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Multi-omics elucidates the kidney damage caused by aquatic Cu via the gut-kidney axis in ducks by Mingcai Duan, Jinyu Liu, Zhaoxia Cai, Li Chen, Yong Tian, Wenwu Xu, Tao Zeng, Tiantian Gu, Lizhi Lu

“…Transcriptomic analysis of kidney tissue reveals that following Cu exposure, 30 genes show significant differential expression. GO and KEGG enrichment analyses were most involved in Interleukin-1 Receptor Activity, Taurine and hypotaurine metabolism, Nitrogen metabolism, and Proximal tubule bicarbonate reclamation. …”
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