The anti-cancerous mechanism of licochalcone A on human hepatoma cell HepG2 based on the miRNA omics by Jun Wang, Xiuxiu Zhang, Zhijing Ni, Elnur Elam, Kiran Thakur, Kexin Li, Chuyan Wang, Jianguo Zhang, Zhaojun Wei

“…To explore the function of licochalcone A as an anticancer phytochemical on HepG2 cells and investigate its potential mechanisms, we analyzed the microRNAs (miRNAs) expression profile of HepG2 cells in response to licochalcone A (70 μmol/L) in vitro. 102 dysregulated miRNAs were detected, and SP1 was expected as the transcription factor that regulates the functions of most screened miRNAs. …”
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Small extracellular vesicles derived from sequential stimulation of canine adipose-derived mesenchymal stem cells enhance anti-inflammatory activity by Saranyou Oontawee, Parkpoom Siriarchavatana, Watchareewan Rodprasert, Irma Padeta, Yudith Violetta Pamulang, Poorichaya Somparn, Trairak Pisitkun, Boondarika Nambooppha, Nattawooti Sthitmatee, Daneeya Na Nan, Thanaphum Osathanon, Hiroshi Egusa, Chenphop Sawangmake

“…Stimulated cAD-MSCs were evaluated for morphological changes, cell viability, and gene expressions. Conditioned media were collected and purified for sEV isolation on Day1, Day2, and Day3. …”
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Hypothermia regulates mitophagy and apoptosis via PINK1/Parkin-VDAC 3 signaling pathway during oxygen-glucose deprivation/recovery injury by Luying Zhang, Song Yang, Hao Cui, Chenchen Hang, Xingsheng Wang, Le An, Zhenyu Shan, Zhen Liang, Rui Shao, Ziren Tang

“…Mitochondrial membrane potential (MMP) and mitochondrial permeability transition pore (mPTP) opening were determined respectively by JC-1 staining and BBcellProbe M61 staining using a flow cytometer. Expression of mitophagy-related proteins (Cleaved PINK1, Parkin, SQSTM1/p62, Beclin-1, LC3B II/LC3B I), apoptosis-related proteins (Bcl-2, Cytochrome C, caspase-3, cleaved caspase3) and VDAC3 were assessed using western blot analysis and quantitative real-time PCR. …”
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METTL16 is Required for Meiotic Sex Chromosome Inactivation and DSB Formation and Recombination during Male Meiosis by Lisha Yin, Nan Jiang, Wenjing Xiong, Shiyu Yang, Jin Zhang, Mengneng Xiong, Kuan Liu, Yuting Zhang, Xinxin Xiong, Yiqian Gui, Huihui Gao, Tao Li, Yi Li, Xiaoli Wang, Youzhi Zhang, Fengli Wang, Shuiqiao Yuan

“…., Ubr2) to regulate the expression of critical meiotic regulators. Collectively, this study identified METTL16 as a key regulator of male meiosis and demonstrated that it modulates meiosis by interacting with MSCI‐related factors and regulating m6A levels and translational efficiency (TE) of meiosis‐related genes.…”
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The Plant-Derived Bauhinia bauhinioides Kallikrein Proteinase Inhibitor (rBbKI) Attenuates Elastase-Induced Emphysema in Mice by Bruno Tadeu Martins-Olivera, Rafael Almeida-Reis, Osmar Aparecido Theodoro-Júnior, Leandro Vilela Oliva, Natalia Neto dos Santos Nunes, Clarice Rosa Olivo, Marlon Vilela de Brito, Carla Máximo Prado, Edna Aparecida Leick, Mílton de Arruda Martins, Maria Luiza Vilela Oliva, Renato Fraga Righetti, Iolanda de Fátima Lopes Calvo Tibério

“…In addition to decreasing mechanical alterations and alveolar septum disruption, rBbKI reduced the number of cells in the BALF and decreased the cellular expression of TNF-α, MMP-9, MMP-12, TIMP-1, eNOS, and iNOS in airways and alveolar walls compared with the ELA group. rBbKI decreased the volume proportion of 8-iso-PGF2α, collagen, and elastic fibers in the airways and alveolar walls compared with the ELA group. …”
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Efficacy and Safety of Bintrafusp Alfa, a Bifunctional Fusion Protein Targeting Transforming Growth Factor-β and Programmed Death-Ligand 1, Plus Chemotherapy in Patients With Stage... by Christian Rolfo, MD, PhD, Laurent Greillier, MD, PhD, Remi Veillon, MD, PhD, Firas Badin, MD, MBA, Francois Ghiringhelli, MD, PhD, Nicolas Isambert, MD, PhD, Astrid Paulus, MD, PhD, Surendra Pal Chaudhary, MD, Yulia Vugmeyster, PhD, Masashi Sato, MSc, Sandrine Hiret, MD, PhD

“…This study evaluated the safety and efficacy of bintrafusp alfa with chemotherapy in patients with stage IV NSCLC regardless of the programmed death-ligand 1 (PD-L1) expression status. Methods: In this open-label, phase 1b/2 study (NCT03840915), eligible patients were assigned to one of four cohorts. …”
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Polyethylene microplastic exposure adversely affects oocyte quality in human and mouse by Qiaoling Wang, Fengli Chi, Yingdong Liu, Qiurong Chang, Siyu Chen, Pengcheng Kong, Wanli Yang, Wenqiang Liu, Xiaoming Teng, Yan Zhao, Yi Guo

“…In conclusion, PE exposure impairs oocyte quality possibly by disrupting follicular fluid metabolism, elevating inflammation-related gene expression, and increasing ROS production in oocytes.…”
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Temperature-dependent toxicity and mechanisms of florfenicol on the embryonic development of marine medaka (Oryzias melastigma) by Xingying Guo, Xu Gong, Jinhui Wang, Tan-Duc Nguyen, Syed Shabi Ul Hassan Kazmi, Jiezhang Mo, Feng Hua, Wenhua Liu, Zhen Wang

“…For genes related to DNA damage and apoptosis (pi3k, akt, foxo1, tp53, bcl-2, bax, apaf-1, caspase 9, and 3), alterations in gene expression were evident for all florfenicol treatments at both 20 °C and 30 °C. …”
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Cytokine Kinetics in Febrile Neutropenic Children: Insights on the Usefulness as Sepsis Biomarkers, Influence of Filgrastim, and Behavior of the IL-23/IL-17 Pathway by Orlei Ribeiro de Araujo, Reinaldo Salomão, Milena Karina Coló Brunialti, Dafne Cardoso Bourguignon da Silva, Andreza Almeida Senerchia, Fabianne Altruda de Moraes Costa Carlesse, Antonio Sergio Petrilli

“…Filgrastim upregulates expression of these markers, and we observed deficiency in the IL-23-IL-17 axis accompanying sepsis.…”
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PD-1 cis-targeted IL-2v in combination with radiotherapy inhibits lung cancer growth and remodels the immune microenvironment by Pedro J Romero, Sara Labiano, Gregory Verdeil, Christian Klein, Pablo Umana, Tania Wyss, Christine Trumpfheller, Marie-Catherine Vozenin, Céline Godfroid, Jackeline Romero, Vincent Roh, Genrich V Tolstonog, Chia-Hsien Chuang, Andrea Kelemen, Laura Codarri Deak

“…The addition of muFAP-CD40 to the combination treatment further increased infiltrating CD8+ T cells, expressing high levels of effector molecules, both in the periphery and core tumor regions. …”
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Insights from the single-cell level: lineage trajectory and somatic-germline interactions during spermatogenesis in dwarf surfclam Mulinia lateralis by Yajuan Li, Huilan Wei, Xiaoting Dai, Lijing Zhang, Liangjie Liu, Xiaomei Chen, Tian Liu, Ya Shu, Yaxin Yang, Shi Wang, Zhenmin Bao, Lingling Zhang

“…Pseudotime analysis indicates that gene expression, translation, ATP metabolic process, and microtubule-based process are involved in the transition of germ cell types. …”
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Edaravone dexborneol alleviates pericyte-mediated fibrosis depositing extracellular matrix through TGF-β1/IL-11 in cerebral small vessel disease by Qingrui Duan, Zhiyang Liu, Yuxuan Xing, Haifeng Huang, Lin Zhu, Jiaxuan Liu, Peikun He, Guixian Ma, Yuhu Zhang, Kun Nie, Yuyuan Gao, Lijuan Wang

“…EDB could improve symptoms and the underlying pathogenesis of CCH mice and decrease the expression of the fibrous profiles of pericytes and ECM proteins, which may be regulated by TGF-β1/ IL-11. …”
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Hydrogel loaded with cerium-manganese nanoparticles and nerve growth factor enhances spinal cord injury repair by modulating immune microenvironment and promoting neuronal regenera... by Zhaoyang Gong, Zhenhao Chen, Dachuan Li, Xiao Lu, Jianwei Wu, Hanqiu Sun, Ximeng Wang, Siyang Liu, Xinlei Xia, Feizhou Lu, Jianyuan Jiang, Chi Sun, Hongli Wang, Feng Zeng, Xiaosheng Ma

“…Notably, the composite significantly upregulated the expression of nerve growth-associated proteins, reduced inflammatory cytokines, scavenged reactive oxygen species (ROS), and promoted M2 macrophage polarization by inhibiting the cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) signaling pathway. …”
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Mediação em centros e museus de ciência no México: um estudo sobre os atores sociais que atuam com os visitantes by Luisa Massarani, Marcela Vitor Alvaro, Alba Patricia Macías-Nestor, Elaine Reynoso-Haynes, María del Carmen Sánchez-Mora, Jessica Norberto Rocha, Willian Vieira de Abreu, Patricia Castellanos Pineda

“…Para os mediadores mexicanos que participaram deste estudo, suas ações não devem se limitar a explicação de conceitos; a maioria deles expressou considerar imprescindível que um bom mediador associe os conteúdos do museu com a vida cotidiana (135; 75,4%) e faça perguntas que provoquem reflexão (121; 67,6%). …”
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The Clinical Characteristics, Patterns of Recurrence, and Long-Term Survival Outcomes of Dual-Phenotype Hepatocellular Carcinoma After Curative Liver Resection by Yu ZC, Fang ZK, Yu Y, Liu SY, Wang KD, Shi ZJ, Jin LM, Huang XK, Lu Y, Shen GL, Liu JW, Huang DS, Zhang CW, Liang L

“…Zi-Chen Yu,1,2 Zheng-Kang Fang,1,2 Yang Yu,3 Si-Yu Liu,4 Kai-Di Wang,1,5 Zhe-Jin Shi,1,5 Li-Ming Jin,1 Xiao-Kun Huang,1,2 Yi Lu,1 Guo-Liang Shen,1 Jun-Wei Liu,1 Dong-Sheng Huang,1 Cheng-Wu Zhang,1 Lei Liang1 1Department of Hepatobiliary & Pancreatic Surgery and Minimally Invasive Surgery, General Surgery, Cancer Center, Zhejiang Provincial People’s Hospital, Affiliated People’s Hospital, Hangzhou Medical College, Hangzhou, Zhejiang, People’s Republic of China; 2Department of Postgraduate Training Base Alliance of Wenzhou Medical University, Wenzhou, Zhejiang, People’s Republic of China; 3Department of Urology, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai, People’s Republic of China; 4Department of Laboratory Medicine, The Key Laboratory of Imaging Diagnosis and Minimally Invasive Interventional Research of Zhejiang Province, Zhejiang University Lishui Hospital, Lishui, Zhejiang, People’s Republic of China; 5Department of the second School of Clinical Medicine, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, People’s Republic of ChinaCorrespondence: Lei Liang, General Surgery, Cancer Center, Department of Hepatobiliary and Pancreatic Surgery and Minimal Invasive Surgery, Zhejiang Provincial People’s Hospital, Affiliated People’s Hospital, Hangzhou Medical College, Hangzhou, Zhejiang, 310014, People’s Republic of China, Email liangl1992@hotmail.comBackground & Aims: Dual-phenotype hepatocellular carcinoma (DPHCC) is discernible from classical HCC (CHCC) in its morphology and is characterized by the co-expression of both CHCC and cholangiocyte markers. This study aimed to clarify the difference between DPHCC and CHCC after surgery.Methods: Patients with HCC after surgery were collected. …”
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Hyperbaric oxygen potentiates platelet-rich plasma composition and accelerates bone healing by Wen-Shuo Chang, Chien-Cheng Huang, Tzu-Hao Chen, Ssu-Han Chao, Cheng-Hsien Lin, Ching-Ping Chang, Chi-Sheng Chien

“…Bone healing was assessed weekly by X-ray, while histological and immunofluorescence analyses evaluated inflammatory status, osteoprotegerin (OPG), receptor activator of nuclear factor kappa-B ligand (RANKL) expression, and the presence of osteoblasts, osteoclasts, and osteocytes during healing. …”
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Absent in melanoma 2: a potent suppressor of retinal pigment epithelial-mesenchymal transition and experimental proliferative vitreoretinopathy by Yu Chen, Mingyuan Jiang, Liping Li, Shanshan Yang, Zuimeng Liu, Shiwen Lin, Wanxiao Wang, Jinyang Li, Feng Chen, Qiang Hou, Xiaoyin Ma, Ling Hou

“…Consequently, Aim2-deficient mice showed morphological changes and increased FN expression in RPE cells under physiological conditions, whereas AIM2 overexpression in phRPE cells inhibited EMT. …”
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Associations between skeletal muscle phenotype, positional role, and on‐ice performance in elite male ice hockey players by Jeppe F. Vigh‐Larsen, Hallur Thorsteinsson, Martin Thomassen, Jeppe Panduro, Bjørn Fristrup, Morten B. Randers, Jens L. Olesen, Peter Krustrup, Kristian Overgaard, Lars Nybo, Magni Mohr

“…Resting thigh muscle biopsies were analyzed for metabolic enzyme activity and protein expression linked to performance. On‐ice intermittent exercise capacity, repeated sprint ability, and maximal isometric knee‐extensor torque were also assessed. …”
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Exposure to nano-polystyrene during pregnancy leads to Alzheimer's disease-related pathological changes in adult offspring by Fengxu Wang, Jin Wang, Na Luo, Yonghua Luo, Zhengnan Gao, Ye Cui, Mengna Jiang, Zhaoping Shen, Jing Xiao, Peng Xue, Xiaoke Wang, Shali Yu, Qiyun Wu, Xinyuan Zhao

“…Furthermore, the phosphorylation levels of tau proteins at ser396 and ser199 were dramatically enhanced in the PS-NPs exposed group. Furthermore, the expression of Aβ protein was markedly increased, consistent with typical AD pathological features. …”
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Breeding and genetic aspects of creating productive forms of fast-developing spring bread wheat by B. V. Rigin, E. V. Zuev, V. A. Tyunin, E. R. Shreyder, Z. S. Pyzhenkova, I. I. Matvienko

“…The spikelet multi-seededness in productive 'Rifor' hybrids is determined by two or three genes and their expression depends on the environment. The selected ultra-early 'Rifor' lines have the number of grains per spike 1,5-2 times higher than in the parent line Rico, but the mass of grain per spike is below that of standard varieties. …”
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