El desarrollo de las capacidades locales de acción a partir de la implementación de estrategias participativas. estudio de caso en el preriurbano de Bahia Blanca by María Prieto, María Amalia Lorda

“…Las ciudades son en estos momentos los territorios en los cuales las transformaciones se expresan con una dinámica constante. Los procesos se aceleran, se multiplican los “encuentros”, y la ciudad se expande a través de los espacios periurbanos que cada una genera. …”
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NUDT21 regulates lysyl oxidase-like 2(LOXL2) to influence ECM protein cross-linking in silica-induced pulmonary fibrosis by Lan Peng, Wenqing Sun, Demin Cheng, Xinying Jia, Wenxiu Lian, Ziwei Li, Haojie Xiong, Ting Wang, Yi Liu, Chunhui Ni

“…Furthermore, we discovered that NUDT21, the LOXL2 upstream regulatory mechanism of LOXL2, alters LOXL2's 3′UTR usage by substituting alternative polyadenylation (APA), thereby modulating LOXL2 expression. By injecting LOXL2 siRNA-loaded liposomes into the tail vein of mice in the silica dust-treated mouse pulmonary fibrosis model, the severity of lung fibrosis was significantly reduced. …”
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Cellular and immune landscape of chronic liver diseases: insights from immunophenotyping by Shaghayegh Soleimani, Shaghayegh Soleimani, Ozgur Albayrak, Kayra Somay, Hong Yang, Buket Yigit, Burge Ulukan, Dila Atak, Murat Akyildiz, Metehan Gursoy, Elif Demirtas, Adil Mardinoglu, Adil Mardinoglu, Atay Vural, Atay Vural, Murat Dayangac, Mujdat Zeybel

“…Additionally, chronic liver disease patients showed higher levels of CD57+ T cells, suggestive of T cell differentiation. Differential expression analysis identified several genes associated with immune regulation, including downregulation of CD27 and upregulation of granzyme B in ARLD, consistent with a highly differentiated phenotype. …”
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Experimental Validation of Antiobesogenic and Osteoprotective Efficacy of Ginsenoside CK via Targeting Lipid and Atherosclerosis Pathways by Md. Niaj Morshed, Reshmi Akter, Imran Mahmud, Ah-Yeong Gwon, Jin Woo Jeang, Yeong-Geun Lee, Dae Won Park, Deok Chun Yang, Yeon Ju Kim, Se-Chan Kang

“…Using 3T3-L1 adipocytes in vitro assessments, CK could effectively decrease intracellular lipid accumulation, inhibit α-glucosidase enzyme, increase 2-NBDG glucose uptake, reduce inflammation-associated cytokines (<i>TNFα</i>, and <i>IL-6</i>), adipogenic regulatory genes (<i>PPARγ</i>, <i>FAS</i>, <i>C/EBPα</i>), lipogenic gene <i>LPL</i>, and increase the expression of thermogenic gene <i>UCP1</i>. Additionally, CK treatment induced osteoblast development in MC3T3-E1 cells as shown by increased mineralization and calcium distribution, collagen content, alkaline phosphatase activity, and decreased inflammatory cytokines <i>TNFα</i>, and <i>IL-6</i> and increased the regulated expressions of osteogenic genes including <i>Runx2</i>, <i>ALP</i>, <i>BGLAP</i>, <i>OCN</i>, and <i>Col1a1</i>. …”
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Prevalence of AZFс Y chromosome microdeletions and association with spermatogenesis in Russian men from the general population by L. V. Osadchuk, G. V. Vasiliev, M. K. Ivanov, M. A. Prasolova, M. A. Kleshchev, A. V. Osadchuk

“…The Y chromosome contains a set of genes with testis-specific expression that are responsible for the development of testes and spermatogenesis, and it is the most important target in the search for genetic causes of male infertility. …”
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Hyperosmotic Stress Induces a Specific Pattern for Stress Granule Formation in Human-Induced Pluripotent Stem Cells by Salam Salloum-Asfar, Rudolf Engelke, Hanaa Mousa, Neha Goswami, I. Richard Thompson, Freshteh Palangi, Kamal Kamal, Muna N. Al-Noubi, Frank Schmidt, Sara A. Abdulla, Mohamed M. Emara

“…We have also unveiled a reduced expression of tubulin that may protect cells against hyperosmolarity stress while inhibiting SG formation without affecting stem cell self-renewal and pluripotency. …”
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mTORC2 Activation Mediated by Mesenchymal Stem Cell-Secreted Hepatocyte Growth Factors for the Recovery of Lipopolysaccharide-Induced Vascular Endothelial Barrier by Shan-Shan Meng, Feng-Mei Guo, Li-Li Huang, Ying-Zi Huang, Jian-Feng Xie, Cong-Shan Yang, Hai-Bo Qiu, Yi Yang

“…Raptor and rictor knockdown in LPS-induced PMEVECs with stimulation of HGF increased apoptosis ratio, activated Cleaved-Caspase-3 expression, and downregulated cell proliferation. Moreover, mTORC2/Akt but not mTORC2/PKC had significance on HGF endothelial protective effects. …”
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HPK1 kinase inhibitor: a sufficient approach to target HPK1 to modulate T cell activation in cancer immunotherapy compared with degraders by Qin Wang, Xinyi Zhu, Jing Li, Sanjia Xu, Ali Wang, Xinwen Zhang, Xingxing Wang, Xiaopeng Cai, Haimei Xing, Ye Liu, Xuesong Liu, Zhiwei Wang, Lai Wang, Lai Wang, Xi Yuan

“…The activation of HPK1-null cells, HPK1-wildtype cells and HPK1-kinase-inactive cells was compared through ectopic expression of HPK1 in HPK1 knockout cells or direct HPK1 mutation. …”
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Shengxue Busui Decoction activates the PI3K/Akt and VEGF pathways, enhancing vascular function and inhibiting osteocyte apoptosis to combat steroid-induced femoral head necrosis by Manting Liu, Jiexiang Ye, Runtian Wu, Dongqiang Luo, Tao Huang, Dandan Dai, Kexin Wang, Yanping Du, Junwen Ou

“…In vitro, SBD inhibited osteoblast apoptosis, promoted PI3K/Akt and VEGF pathway expression, and enhanced osteoblast differentiation and mineralization.ConclusionThis study integrates network pharmacology with experimental validation, showing that SBD protects against SONFH by inhibiting osteoblast apoptosis via PI3K/Akt and VEGFR pathways. …”
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NLRX1 limits inflammatory neurodegeneration in the anterior visual pathway by Alexander J. Gill, Matthew D. Smith, Danny Galleguillos, Thomas Garton, Jackson W. Mace, Sachin P. Gadani, Swati Kumar, Aayush Pokharel, Krista Solem, Saahith Potluri, Omar Hussein, Giuliana Sardi Rogines, Arihant Singh, Annatje Clark, Peter A. Calabresi, Marjan Gharagozloo

“…The novel pharmacologic NLRX1 activators NX-13 and LABP-66 decreased LPS-mediated gene expression of inflammatory cytokines and chemokines in mixed glial cultures. …”
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Gravity-based microfiltration reveals unexpected prevalence of circulating tumor cell clusters in ovarian and colorectal cancer by Anne Meunier, Javier Alejandro Hernández-Castro, Nicholas Chahley, Laudine Communal, Sara Kheireddine, Newsha Koushki, Nadia Davoudvandi, Sara Al Habyan, Benjamin Péant, Anthoula Lazaris, Andy Ng, Teodor Veres, Luke McCaffrey, Diane Provencher, Peter Metrakos, Anne-Marie Mes-Masson, David Juncker

“…We perform GµF of blood from orthotopic ovarian cancer mouse models and characterize the morphological features of scCTCs and cCTCs, and the expression of molecular markers for aggressiveness. …”
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Cortical AAV-CNTF Gene Therapy Combined with Intraspinal Mesenchymal Precursor Cell Transplantation Promotes Functional and Morphological Outcomes after Spinal Cord Injury in Adult... by Stuart I. Hodgetts, Jun Han Yoon, Alysia Fogliani, Emmanuel A. Akinpelu, Danii Baron-Heeris, Imke G. J. Houwers, Lachlan P. G. Wheeler, Bernadette T. Majda, Sreya Santhakumar, Sarah J. Lovett, Emma Duce, Margaret A. Pollett, Tylie M. Wiseman, Brooke Fehily, Alan R. Harvey

“…Cortical neurons were transduced with a bicistronic adeno-associated viral vector (AAV1) expressing a secretory form of CNTF coupled to mCHERRY (AAV-CNTFmCherry) or with control AAV only (AAV-GFP) two weeks prior to SCI. …”
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Complete pathway elucidation of echinacoside in Cistanche tubulosa and de novo biosynthesis of phenylethanoid glycosides by Wenqian Huang, Yaru Yan, Weisheng Tian, Xiaoxue Cui, Yingxia Wang, Yuelin Song, Ting Mo, Xiping Xu, Saijing Zhao, Yuyu Liu, Xiaohui Wang, Juan Wang, Yong Jiang, Jun Li, She-po Shi, Xiao Liu, Pengfei Tu

“…Furthermore, the de novo synthesis of 23 PhG derivatives is achieved via the heterologous expression of different combinations of the functional genes in tobacco. …”
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Xi Lei San Attenuates Dextran Sulfate Sodium-Induced Colitis in Rats and TNF-α-Stimulated Colitis in CACO2 Cells: Involvement of the NLRP3 Inflammasome and Autophagy by Zhang Tao, Xiaoqing Zhou, Yan Zhang, Wenfeng Pu, Yi Yang, Fuxia Wei, Qian Zhou, Lin Zhang, Zhonghan Du, Ji Wu

“…A western blot analysis was performed to determine the expression levels of inflammasome-related proteins and autophagy-related proteins, ELISA was performed to analyze IL-1β, IL-18, and IL-33 concentrations, and flow cytometry was used to monitor cell apoptosis and ROS levels. …”
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Targeting sphingosine 1-phosphate receptor 3 inhibits T-cell exhaustion and regulates recruitment of proinflammatory macrophages to improve antitumor efficacy of CAR-T cells agains... by Weiting Liao, Ge Gao, Xia He, Pei Shu, Qizhi Ma, Benxia Zhang, Diyuan Qin, Yongsheng Wang

“…We have applied RNA sequencing, flow cytometry, ELISA, cellular/molecular immunological technology, and mouse models of solid cancers.Results Our study provided evidence that S1PR3 high expression is positively associated with resistance to programmed cell death protein-1 (PD-1)-based immunotherapy and increased T-cell exhaustion. …”
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Epigenetic regulation in coronary artery disease: from mechanisms to emerging therapies by Rui Gao, Rui Gao, Rui Gao, Meilin Liu, Meilin Liu, Meilin Liu, Haoyi Yang, Haoyi Yang, Haoyi Yang, Yuhan Shen, Yuhan Shen, Yuhan Shen, Ni Xia, Ni Xia, Ni Xia

“…Epigenetics is a field investigating heritable alterations in gene expression without DNA sequence changes, such as DNA methylation, histone modifications, and non-coding RNAs. …”
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Clinical features and genetic analysis of 15 Chinese children with dent disease by Qian Li, Zhenle Yang, Ruixian Zang, Suwen Liu, Lichun Yu, Jing Wang, Cong Wang, Xiaoyuan Wang, Shuzhen Sun

“…Objective  The clinical characteristics, genetic mutation spectrum, treatment strategies and prognoses of 15 children with Dent disease were retrospectively analyzed to improve pediatricians’ awareness of and attention to this disease.Methods  We analyzed the clinical and laboratory data of 15 Chinese children with Dent disease who were diagnosed and treated at our hospital between January 2017 and May 2023 and evaluated the expression of the CLCN5 and OCRL1 genes.Results  All 15 patients were male and complained of proteinuria, and the incidence of low-molecular-weight proteinuria (LMWP) was 100.0% in both Dent disease 1 (DD1) and Dent disease 2 (DD2) patients. …”
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Machine learning based on blood test biomarkers predicts fast progression in advanced NSCLC patients treated with immunotherapy by Jie Yang, Jian-Guo Zhou, Benjamin Frey, Hu Ma, Haitao Wang, Markus Hecht, Rainer Fietkau, Udo Gaipl, Xiaofei Chen, Ada Hang-Heng Wong, Fangya Tan, Si-Si He, Gang Shen, Yun-Jia Wang, Wenzhao Zhong

“…In addition, the absolute difference in median survival between the SVM-predicted FP and non-FP groups was significant in both progression-free survival and overall survival (p&lt;0.001).Conclusion SVM trained using a 4-biomarker panel has good performance in predicting the occurrence of FP regardless of programmed cell death ligand 1 expression, hence providing evidence for decision-making in single-agent atezolizumab immunotherapy for patients with advanced NSCLC.…”
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UHPLC-QTOF-MS/MS profiling, molecular networking, and molecular docking analysis of Gliricidia sepium (Jacq.) Kunth. ex. Walp. stem ethanolic extract and its gastroprotective effec... by Aya A. Wafaey, Seham S. El-Hawary, Osama G. Mohamed, Sahar S. Abdelrahman, Alaa M. Ali, Ahmed A. El-Rashedy, Mohamed F. Abdelhameed, Farid N. Kirollos

“…Additionally, it enhanced superoxide dismutase and inhibitor of nuclear factor kappa B alpha levels, while lowering the expression of inducible nitric oxide synthase. Histopathological examination revealed significant improvements in gastric tissue morphology in GSS-treated groups compared to the control group. …”
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