Phosphorylation of Arabidopsis NRT1.1 regulates plant stomatal aperture and drought resistance in low nitrate condition by Yuchen Kou, Bodan Su, Shunyao Yang, Wei Gong, Xi Zhang, Xiaoyi Shan

“…Results In this study, we observed that seedlings expressing the phosphorylated form of NRT1.1 (NRT1.1T101D, T101D) exhibited increased drought tolerance compared to dephosphorylated NRT1.1 (NRT1.1T101A, T101A) mutants under low nitrate (LN) condition, characterized by decreased stomatal aperture and water loss. …”
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Heat-not-burn technology affects plasma testosterone levels and markers of inflammation, oxidative stress in the testes of rats by Silvia Granata, Camilla Morosini, Maria Chiara Valerii, Ivan Fagiolino, Stefano Sangiorgi, Severino Ghini, Enzo Spisni, Fabio Vivarelli, Lucy C. Fairclough, Moreno Paolini, Donatella Canistro

“…Although the levels of toxicants in the mainstream are significantly lower than those observed in tobacco smoke, some recent studies have raised concerns about potential health risks associated with their use, particularly regarding their effects on male gonadal function, which remain largely unexplored.MethodsAdult male Sprague-Dawley rats were exposed, whole body, 5 days/week for 4 weeks to HnB mainstream.ResultsThe expression of the cell cycle regulators Bax/Bcl-2 ratio is not affected, along with no changes in p-38. …”
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Cardiorenal Involvement in Metabolic Syndrome Induced by Cola Drinking in Rats: Proinflammatory Cytokines and Impaired Antioxidative Protection by Matilde Otero-Losada, Hernán Gómez Llambí, Graciela Ottaviano, Gabriel Cao, Angélica Müller, Francisco Azzato, Giuseppe Ambrosio, José Milei

“…Compared with W, C rats showed (I) overweight (+8%, p<0.05), hyperglycemia (+11%, p<0.05), hypertriglyceridemia (2-fold, p<0.001), higher AIP (2-fold, p<0.01), and lower Q10 level (−55%, p<0.05); (II) increased LV diastolic diameter (+9%, p<0.05) and volume (systolic +24%, p<0.05), posterior wall thinning (−8%, p<0.05), and larger cardiac output (+24%, p<0.05); (III) glomerulosclerosis (+21%, p<0.05), histopathology (+13%, p<0.05), higher tubular expression of IL-6 (7-fold, p<0.001), and TNFα (4-fold, p<0.001). …”
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MCU complex: Exploring emerging targets and mechanisms of mitochondrial physiology and pathology by Jin Wang, Jinyong Jiang, Haoliang Hu, Linxi Chen

“…We also studied the functional expression of the MCU complex in Drosophila, Zebrafish, and Caenorhabditis elegans. …”
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CD137 Signaling Promotes Endothelial Apoptosis by Inhibiting Nrf2 Pathway, and Upregulating NF-κB Pathway by Tianxin Geng, Yang Yan, Yue Zhang, Liangjie Xu, Guangyao Zang, Jin Chuan Yan

“…Confocal microscopy and Western blot analysis showed that the nuclear translocation of Nrf2 in the agonist-CD137 group was significantly inhibited, and the expression of its downstream antioxidant enzymes was also decreased when compared with control. …”
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MCT1 lactate transporter blockade re-invigorates anti-tumor immunity through metabolic rewiring of dendritic cells in melanoma by Camille Niveau, Mélanie Cettour-Cave, Stéphane Mouret, Eleonora Sosa Cuevas, Mylene Pezet, Benoît Roubinet, Hugo Gil, Florence De Fraipont, Ludovic Landemarre, Julie Charles, Philippe Saas, Caroline Aspord

“…We demonstrate that circulating and tumor-infiltrating DC subsets from melanoma patients are characterized by altered metabolism, which is linked to their activation status and profile of immune checkpoint expression. This altered metabolism influences their function and affects patient clinical outcome. …”
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Analysis of five cases showing false-high Hemoglobin A1c due to reduced catalase activity by Kenji Hara, Atsushi Ujiie, Shiori Suzuki, Takenori Okumura, Mio Kubo, Hiroyuki Shinozaki, Mototaka Yamauchi, Takafumi Tsuchiya, Kohzo Takebayashi, Nobuyuki Shimozawa, Masafumi Koga, Koshi Hashimoto

“…Catalase activity and gene mutations were examined in the five cases and an immunohistological analysis was performed to assess the expression of catalase. Cases #1 and 2 were diagnosed as acatalasemia and cases #3, 4, and 5 as hypocatalasemia based on compound heterozygous SNP and heterozygous splicing mutations in the catalase gene. …”
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A Circulating Subpopulation of Monocytic Myeloid-Derived Suppressor Cells as an Independent Prognostic/Predictive Factor in Untreated Non-Small Lung Cancer Patients by Eleni-Kyriaki Vetsika, Filippos Koinis, Marianthi Gioulbasani, Despoina Aggouraki, Anna Koutoulaki, Eirini Skalidaki, Dimitris Mavroudis, Vassilis Georgoulias, Athanasios Kotsakis

“…Two monocytic [CD14+CD15−CD11b+CD33+HLA-DR−Lin− and CD14+CD15+CD11b+CD33+HLA-DR−Lin−] and a granulocytic [CD14−CD15+CD11b+CD33+HLA-DR−Lin−] subpopulations of MDSCs were identified, expressing inducible nitric oxide synthase, and reactive oxygen species, respectively. …”
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Effect of custom-designed transfection buffer on delivery of genome modification components into primary cells of buffalo, cattle, goats, and sheep by Shreya Malhotra, Priyanka Singh, Aseem Tara, Bosco Jose, Devika Gautam, Ram Prasad, Gaurav Tripathi, Sacchinandan De, Naresh L. Selokar

“…To achieve the aims of this study, t primary somatic cells from skin biopsies were established and were transfected with a Venus-expression vector (pAcGFPs-Venus). We noticed that transfection rates of pAcGFPs-Venus were 22.51%, 17.56%, 22.81%, and 16.16% for buffalo, cattle, goats, and sheep cells, respectively. …”
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Targeted microbiome metabolomics reveals flaxseed oil supplementation regulated the gut microbiota and farnesoid X receptor pathway in high-fat diet mice by Chen Yang, Zhenxia Xu, Qingde Huang, Xu Wang, Fenghong Huang, Qianchun Deng

“…Further analysis revealed that FSO activated FXR in the liver and regulated downstream gene expression (SHP, SREBP-1c, and CPT-1a), which promoted lipolysis and inhibited lipogenesis. …”
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Thiacloprid exposure disrupts the gut-liver axis and induces liver dysfunction in the Reeves' turtles (Mauremys reevesii) by Shuqin Lin, Yunjuan Xiao, Siyu Li, Liyan Tang, Haitao Shi, Meiling Hong, Li Ding

“…Metabolomic and transcriptomic analyses indicated that metabolic dysregulation and differences in gene expression were concentrated in amino acid metabolism and lipid metabolism, ultimately resulting in severe liver damage and steatosis. …”
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CircCCT2/miR-146a-5p/IRAK1 axis promotes the development of head and neck squamous cell carcinoma by Long He, Lanruo Li, Liting Zhao, Xiaoya Guan, Yujia Guo, Qi Han, Huina Guo, Hongliang Liu, Chunming Zhang

“…Circular RNAs (circRNAs) have become key regulators of human malignancies, but further studies are necessary to fully understand their functions and possible causes in HNSCC. Methods CircCCT2 expression levels in HNSCC tissues and cells were measured via qPCR. …”
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Pioglitazone Metformin Complex Improves Polycystic Ovary Syndrome Comorbid Psychological Distress via Inhibiting NLRP3 Inflammasome Activation: A Prospective Clinical Study by Qing-jun Guo, Jing Shan, Yi-feng Xu, Yan-yan Hu, Cui-lan Huo, Jing-yun Song, Chao-qun Wang, Hui Zhou, Chao-qin Yu, Qin Huang

“…PM significantly decreased the expression of NRPL3 and caspase-1. Patients in the PM group experienced a significant reduction in IL-1β (from 98.42±14.38 to 71.76±13.66, p=0.02), IL-6 (from 87.51±8.74 to 71.98±15.87, p=0.02), and TNF-α (from 395.33±88.55 to 281.98±85.69, p=0.04). …”
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Ginger protects against vein graft remodeling by precisely modulating ferroptotic stress in vascular smooth muscle cell dedifferentiation by Xiaoyu Yu, Weiwei Wu, Jingjun Hao, Yuxin Zhou, Deyang Yu, Wei Ding, Xuejuan Zhang, Gaoli Liu, Jianxun Wang

“…Furthermore, both ingredients co-target peroxisome proliferator-activated receptor gamma (PPARγ), decreasing PPARγ-mediated nicotinamide adenine dinucleotide phosphate (NADPH) oxidase 1 (Nox1) expression. Nox1 promotes intracellular reactive oxygen species (ROS) production and directly induces VSMC dedifferentiation. …”
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Annexin V+ Microvesicles in Children and Adolescents with Type 1 Diabetes: A Prospective Cohort Study by Vibeke Bratseth, Hanna D. Margeirsdottir, Gemma Chiva-Blanch, Martin Heier, Svein Solheim, Harald Arnesen, Knut Dahl-Jørgensen, Ingebjørg Seljeflot

“…In the type 1 diabetes group, the total AV+, tissue factor-expressing AV+/CD142+, neutrophil-derived AV+/CD15+ and AV+/CD45+/CD15+, and endothelial-derived AV+/CD309+ and CD309+/CD34+ cMVs were inversely correlated with HbA1c (r=‐0.437, r=‐0.515, r=‐0.575, r=‐0.529, r=‐0.416, and r=‐0.445, respectively; all p≤0.01), however, only at inclusion. …”
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Infiltration of Blood-Derived Macrophages Contributes to the Development of Diabetic Neuropathy by Jing-Jing Sun, Lin Tang, Xiao-Pei Zhao, Jun-Mei Xu, Yang Xiao, Hui Li

“…The depletion of MDMs by clodronate liposomes alleviated diabetes-induced tactile allodynia (P<0.05) and reduced the infiltration of MDMs (P<0.001) as well as the expression of IL-1β and TNF-α in the spinal cord (P<0.05). …”
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Investigation of calonysterone and 20-hydroxyecdysone effects in high-fat, high-sugar diet-induced obesity rat model by Alaa AM. Osman, Dávid Laczkó, Máté Vágvölgyi, Adrienn Seres-Bokor, Anita Sztojkov-Ivanov, Kata Kira Kemény, Attila Hunyadi, Eszter Ducza

“…Our ongoing research examines the impacts of 20E and calonysterone (CAL) in an animal model with a diet high in fats and sugars (HFHSD).Glucose tolerance tests assessed prediabetic status and RT-PCR and Western blot analysis determined interleukin-6 (IL6) expression. The concertations of superoxide dismutase, catalase, adiponectin, leptin, and IL-6 were quantified by ELISA. …”
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On writing about illness in Kuwait. A discussion with Shahd Al Shammari by Marion Breteau

“…It can also become a “politics of love” when expressing pain and anger is less important than raising awareness and inviting to solidarity through writing and teaching. …”
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Malvidin-3-galactoside from blueberry suppresses the growth and metastasis potential of hepatocellular carcinoma cell Huh-7 by regulating apoptosis and metastases pathways by Jie Lin, Jinlong Tian, Chi Shu, Zhen Cheng, Yunen Liu, Weisheng Wang, Ruihai Liu, Bin Li, Yuehua Wang

“…Regarding the metastasis potential, Mv-3-gal suppressed the migration and invasion potential of Huh-7 cells by regulating MMPs expression. Taken together, the findings highlighted the anti-HCC potent of Mv-3-gal, which was associated with the inhibition of Akt/PTEN, MAPK and MMP pathways.…”
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Incorporating insulin into alginate-chitosan 3D-printed scaffolds: A comprehensive study on structure, mechanics, and biocompatibility for cartilage tissue engineering by Afsaneh Jahani, Mohammad Sadegh Nourbakhsh, Ali Moradi, Marzieh Mohammadi, Lobat Tayebi

“…The findings revealed that the incorporation of a 20 (μg/ml) dose of Insulin into Alg-Cs 3D-printed scaffolds significantly enhanced the expression of these markers, indicating improved chondrogenic potential for cartilage regeneration. …”
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