Electroacupuncture Pretreatment Elicits Tolerance to Cerebral Ischemia/Reperfusion through Inhibition of the GluN2B/m-Calpain/p38 MAPK Proapoptotic Pathway by Bao-yu Zhang, Guan-ran Wang, Wen-hua Ning, Jian Liu, Sha Yang, Yan Shen, Yang Wang, Meng-xiong Zhao, Li Li

“…Model preparation for MCAO/R or sham MCAO/R started 2 hours after the last pretreatment. 24 hours after model preparation, the Garcia neurobehavioral scoring criteria was used for the evaluation of neurological deficits, TTC for the measurement of infarct volume, TUNEL staining for determination of neural cell apoptosis at hippocampal CA1 area, and WB and double immunofluorescence staining for expression and the cellular localization of GluN2B and m-calpain and p38 MAPK. …”
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Signalisation multimodale de la gestation chez le macaque japonais (Macaca fuscata) by Cécile Garcia, Lucie Rigaill, Andrew JJ. MacIntosh, James P. Higham, Sandra Winters, Keiko Shimizu, Keiko Mouri, Takeshi Furuichi

“…Le rôle des multiples signaux sexuels dans l’indication du timing de l’ovulation des femelles ainsi que la discrimination de ce timing par les mâles ont été particulièrement bien étudiés chez les primates. Cependant, l’expression de signaux relatifs à la gestation et la façon dont ces signaux peuvent moduler les décisions d’accouplement post-conception des mâles sont encore peu comprises. …”
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Scent of COVID-19: Whole-Genome Sequencing Analysis Reveals the Role of <i>ACE2</i>, <i>IFI44</i>, and <i>NDUFAF4</i> in Long-Lasting Olfactory Dysfunction by Beatrice Spedicati, Alessandro Pecori, Maria Pina Concas, Aurora Santin, Romina Ruberto, Giuseppe Giovanni Nardone, Andrea D’Alessandro, Giancarlo Tirelli, Paolo Boscolo-Rizzo, Giorgia Girotto

“…Considering the physiological role of the proteins encoded by these genes, it can be hypothesized that a reduced expression of <i>ACE2</i> may be associated with a protracted and severe inflammatory response in the olfactory epithelium, thus worsening patients’ smell abilities. …”
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Inhibition of NLRP3 enhances pro-apoptotic effects of FLT3 inhibition in AML by Helene Sieberer, Michela Luciano, Diana Amend, Constantin Blöchl, Anna Eglseer, Alina Steinkellner, Sebastian Rieser, Ancuela Andosch, Philip Steiner, Laura Hummer, Peter W. Krenn, Hieu-Hoa Dang, Christian G. Huber, Fritz Aberger, Theresa Neuper, Jutta Horejs-Hoeck

“…Moreover, we show that NLRP3 inhibition impairs FLT3 signaling by reducing both FLT3 expression as well as downstream signaling in FLT3 mutant cells. …”
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Spatial proteomics identifies a CRTC-dependent viral signaling pathway that stimulates production of interleukin-11 by Benjamin J. Ravenhill, Marisa Oliveira, George Wood, Ying Di, Joanne Kite, Xinyue Wang, Colin T.R. Davies, Yongxu Lu, Robin Antrobus, Gill Elliott, Nerea Irigoyen, David J. Hughes, Paul A. Lyons, Betty Chung, Georg H.H. Borner, Michael P. Weekes

“…Many of them exhibit subcellular relocalization upon activation, resulting in the expression of interferon and antiviral genes. To comprehensively identify signaling factors, we analyzed protein relocalization on a global scale during viral infection. cAMP-responsive element-binding protein (CREB)-regulated transcription coactivators 2 and 3 (CRTC2/3) exhibited early cytoplasmic-to-nuclear translocation upon infection with multiple viruses in diverse cell types. …”
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Subjective cognition in schizophrenia and bipolar disorder: Investigation of group differences and associations with objective cognition and clinical characteristics using a novel... by Kristoffer Grimstad, Håkon Sørensen, Anja Vaskinn, Christine Mohn, Stine Holmstul Olsen, Ole A. Andreassen, Trine Vik Lagerberg, Ingrid Melle, Merete Glenne Øie, Torill Ueland, Beathe Haatveit

“…Our results suggest that the expression of subjective cognition is associated with different clinical factors in SZ and BD.…”
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Adeno-associated virus-based gene therapy for hemophilia–addressing the gaps by Wolfgang Miesbach, Paul Batty, Pratima Chowdary, Sylvia Fong, Radoslaw Kaczmarek, Frank W.G. Leebeek, Brian Long, Johnny Mahlangu, Mike Makris, Glenn F. Pierce, Steven W. Pipe, Alok Srivastava, Jan Voorberg, Flora Peyvandi

“…This manuscript follows literature reviews and structured discussions by the International Society on Thrombosis and Haemostasis Scientific and Standardization Committee Working Group on Gene Therapy that identified specific areas requiring standardization of methods, including viral vector production, liver function assessment, quantification of factor (F)VIII and FIX expression levels, assessment of antiadeno-associated viral antibodies, and genomic integration detection methods. …”
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The hypertrophic cardiomyopathy-associated A331P actin variant enhances basal contractile activity and elicits resting muscle dysfunction by Matthew H. Doran, Michael J. Rynkiewicz, Evan Despond, Meera C. Viswanathan, Aditi Madan, Kripa Chitre, Axel J. Fenwick, Duncan Sousa, William Lehman, John F. Dawson, Anthony Cammarato

“…Transgenic Drosophila, expressing A331P actin, displayed skeletal muscle hypercontraction and elevated basal myocardial activity. …”
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Prospective and Longitudinal Analysis of Lymphocyte Subpopulations in SARS-CoV-2 Positive and Negative Pneumonia: Potential Role of Decreased Naïve CD8⁺ in COVID-19 Pati... by Makhabbat Bekbossynova, Lyudmila Akhmaltdinova, Kuanysh Dossybayeva, Ainur Tauekelova, Zauresh Smagulova, Tatyana Tsechoeva, Gulsimzhan Turebayeva, Aliya Sailybayeva, Zhanar Kalila, Takhmina Mirashirova, Timur Muratov, Dimitri Poddighe

“…When B and T cells were analyzed according to the expression of memory markers, a significant decrease in naïve CD8⁺ T cells was observed in the SARS-CoV-2-positive pneumonia group during the acute phase. …”
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Dynamic Complement Protein Changes in Aqueous Humor and Plasma of Patients With Retinal Vein Occlusion During Ranibizumab Treatment by Guo T, Zhao Y, Liang S, Wang J, Liu H, Zhou Y, Xu H, Chen Z

“…Tingting Guo,1,2,&ast; Yanying Zhao,2,3,&ast; Shengnan Liang,2,3 Jie Wang,2 Hengwei Liu,2 Yufan Zhou,2 Heping Xu,3– 5 Zhongping Chen1– 3,6 1Aier Eye Hospital, Jinan University, Guangzhou, People’s Republic of China; 2Changsha Aier Eye Hospital, Changsha, People’s Republic of China; 3Aier Academy of Ophthalmology, Central South University, Changsha, People’s Republic of China; 4Aier Institute of Optometry and Vision Science, Aier Eye Hospital Group, Changsha, People’s Republic of China; 5The Wellcome-Wolfson Institute for Experimental Medicine, School of Medicine, Dentistry and Biomedical Sciences, Queen’s University Belfast, Belfast, UK; 6School of Stomatology and Ophthalmology, Xianning Medical College, Xianning, People’s Republic of China&ast;These authors contributed equally to this workCorrespondence: Zhongping Chen; Heping Xu, Email chenzhongping@csu.edu.cn; heping.xu@qub.ac.ukPurpose: To assess dynamic changes of complement protein in aqueous humor (AH) and plasma of retinal vein occlusion (RVO) patients during ranibizumab treatment, and to explore the differential expression of complement proteins in branch retinal vein occlusion (BRVO) and central retinal vein occlusion (CRVO).Patients and Methods: This prospective, consecutive case series study collected AH and plasma samples from 27 RVO patients at baseline, 1 and 2 months after ranibizumab treatment, including 19 BRVO and 8 CRVO patients. …”
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Impact of meropenem exposure on fluoroquinolone and carbapenem resistance in Pseudomonas aeruginosa infection in inpatients in a Japanese university hospital: Insights into oprD mu... by Tadanori Yamochi, Kazuhisa Ugajin, Rintaro On, Sho Inoue, Hiromi Ikeda, Toshiko Yamochi, Masafumi Takimoto, Issei Tokimatsu

“…Quantitative PCR was performed to analyze oprD, mexB, mexC, mexE, and mexY expression. DNA sequencing was performed to identify mutations. …”
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Nicotinamide Mononucleotide Restores NAD⁺ Levels to Alleviate LPS-Induced Inflammation via the TLR4/NF-κB/MAPK Signaling Pathway in Mice Granulosa Cells by Mehboob Ahmed, Umair Riaz, Haimiao Lv, Muhammad Amjad, Sohail Ahmed, Shaokat Ali, Muhammad Usman Ghani, Guohua Hua, Liguo Yang

“…On the other hand, NMN supplementation restored the NAD⁺ levels and the NAD⁺/NADH ratio in GCs and significantly reduced the expression of pro-inflammatory markers at both mRNA and protein levels. …”
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DNA Damage Sensor γ-H2AX Is Increased in Preneoplastic Lesions of Hepatocellular Carcinoma by Yasunobu Matsuda, Toshifumi Wakai, Masayuki Kubota, Mami Osawa, Masaaki Takamura, Satoshi Yamagiwa, Yutaka Aoyagi, Ayumi Sanpei, Shun Fujimaki

“…All cases with chronic liver disease showed increased levels of γ-H2AX expression. In 40 (69.9%) of 58 cases with HCC, the labeling index (LI) of γ-H2AX was above 50% and was inversely correlated with the histological grade. …”
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Development of a stable eukaryotic strain producing fully human monoclonal antibody on the basis of the human antibody against ectromelia virus by A. L. Matveev, Ya. A. Khlusevich, I. K. Baykov, I. V.  Babkin, E. P. Goncharova, V. V. Morozova, N. V. Tikunova

“…The aim of this study was the development of an original expression system, using gene targeting to integrate the gene encoding the fully­human antibody into the transcriptionally active region of the genome of eukaryotic suspension cells CHO­S. …”
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A Comparison of Classification Algorithms for Predicting Dis-tinctive Characteristics in Fine Aroma Cocoa Flowers Using WE-KA Modeler by Daniel Tineo, Yuriko S. Murillo, Mercedes Marín, Darwin Gomez, Victor H. Taboada, Malluri Goñas, Lenin Quiñones Huatangari

“… The expression of crop functional traits is influenced by environmental and management conditions, which in turn is reflected in genetic diversity. …”
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Puerarin Ameliorates the Ferroptosis in Diabetic Liver Injure Through the JAK2/STAT3 Pathway Inhibition Based on Network Pharmacology and Experimental Validation by Fan X, Liu S, Yu J, Hua J, Feng Y, Wang Z, Shen Y, Lan W, Wang J

“…Western blotting results showed that Puerarin could regulate the expression of proteins related to JAK2/STAT3 pathway and ferroptosis pathway. …”
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Combined mutations in nonstructural protein 14, envelope, and membrane proteins mitigate the neuropathogenicity of SARS-CoV-2 Omicron BA.1 in K18-hACE2 mice by Kotou Sangare, Shufeng Liu, Prabhuanand Selvaraj, Charles B. Stauft, Matthew F. Starost, Tony T. Wang

“…Given that Nsp14(I42V), E(T9I), M(Q19E/A63T) are well preserved in subsequent omicron subvariants, including currently circulating variants, our findings highlight the importance of understanding how non-spike mutations affect the pathogenicity of SARS-CoV-2 variants.IMPORTANCEInoculation of transgenic mice expressing human angiotensin-converting enzyme 2 (hACE2) with SARS-CoV-2 often leads to a fatal brain infection. …”
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Scutellarin Prevents Angiogenesis in Diabetic Retinopathy by Downregulating VEGF/ERK/FAK/Src Pathway Signaling by Lingli Long, Yubin Li, Shuang Yu, Xiang Li, Yue Hu, Tengfei Long, Liqin Wang, Wenwen Li, Xiaoxin Ye, Zunfu Ke, Haipeng Xiao

“…These results suggested that SCU can be an oral drug to alleviate microvascular dysfunction of DR and exerts its antiangiogenic effects by inhibiting the expression of the crosstalk of VEGF, p-ERK, p-FAK, and p-Src.…”
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New prognostic model in patients with advanced urothelial carcinoma treated with second-line immune checkpoint inhibitors by Ernest Choy, Yohann Loriot, Fabio Calabrò, Cora N Sternberg, Guillermo De Velasco, Daniel Castellano, Roubini Zakopoulou, Aristotelis Bamias, Nicholas James, Axel Merseburger, F Lopez-Rios, Mario Kramer, Kimon Tzannis

“…Inclusion of PD-L1 expression did not improve the model.Conclusions We developed and internally validated a prognostic model for patients with aUC receiving postplatinum immunotherapy. …”
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The role of natural exosomes from SHED-MSC in immunoregulation of M0/M1 polarized macrophage cells by Ali Fallah, Abasalt Hosseinzadeh Colagar, Ayyoob Khosravi, Ayyoob Khosravi, Azadeh Mohammad-Hasani, Azadeh Mohammad-Hasani, Mohsen Saeidi, Mohsen Saeidi

“…This study focused on exosomes derived from stem cells of human exfoliated deciduous teeth (SHED-MSC-EXOs) and their role in managing the balance of immunoregulatory macromolecules that play a role in the underlying immunoregulatory mechanisms in THP-1-derived M0/M1 macrophage cells.MethodsFlow cytometry confirmed the expression of CD14, CD68, CD80, and CD86 markers in these macrophages. …”
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